Orientador: Rafael Silva Oliveira / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-20T21:06:58Z (GMT). No. of bitstreams: 1 Candido_HugoGalvao_M.pdf: 2416852 bytes, checksum: e2c495e1dc8a3aa3997d789f5761655e (MD5) Previous issue date: 2012 / Resumo: A disponibilidade de nutrientes tem um papel fundamental na estruturação de comunidades vegetais. Um modelo teórico recentemente proposto por Lambers et al. 2008 postula que em regiões com solos muito antigos e pobres em fósforo e nitrogênio, as espécies dominantes teriam maior proporção de especializações radiculares e menos associações com fungos micorrízicos. Estas regiões foram classificadas como OCBILs (do inglês: old, climatically buffered, infertile landscapes). Este estudo teve como objetivo testar tal modelo e avaliar se comunidades de campos rupestres podem ser categorizadas como OCBILs, avaliar se os campos rupestres podem ser considerados OCBILs mediante uma análise comparativa de um conjunto de caracteres morfofisiológicos das plantas e características físico-químicas do solo, através do estudo de seis comunidades na Cadeia do Espinhaço, Minas Gerais. Selecionamos as dez espécies mais abundantes em cada uma áreas distribuídas ao longo de um transecto de 190 km, totalizando 60 espécies. Avaliamos os atributos foliares (concentração de N, P, análise da massa foliar por unidade de área e razão N:P) e a colonização de raízes por micorrizas. Quimicamente, os solos dos campos rupestres têm baixa disponibilidade de P e N, comparáveis aos solos de outros OCBILs (oeste da Austrália e região do Cabo na África do Sul). A proporção de colonização das raízes das plantas por fungos foi positivamente correlacionada com a [P] do solo de cada comunidade, corroborando o modelo de Lambers em uma escala regional. Espécies de campos rupestres apresentam uma alta razão N:P foliar (média de 43 para todos os campos de estudo), maior que as encontradas nos outros OCBILs, o que sugere uma forte limitação por fósforo. Foram encontradas médias de 183 g m-2 para LMA, 12,46 mg g-1 para o N foliar e de 0,36 mg g-1 para o P foliar. Pelos resultados obtidos propomos que os campos rupestres devem ser classificados também como OCBILs, status que demandaria maior atenção para esses campos ainda pouco estudados e já ameaçados ecologicamente / Abstract: The availability of nutrients plays a key role in structuring plant communities. A theoretical model recently proposed by Lambers et al. (2008) postulates that in regions with very old and poor soils in P and N, the dominant species had a higher proportion of specializations and less root associations with mycorrhizal fungi. These regions were classified as OCBILs (ols, climatically buffered, infertile landscapes). This study aims to test this model and asses whether communities of rupestrian fields might be categorized as OCBILs by analyzing the chemical composition of soil, leaf stoichiometry and % mycorrhizal root colonization by the dominant species in six communities in the Espinhaço Range, Minas Gerais. We selected the ten most abundant species in each of areas distributed along a transect of 190 km, totaling 60 species. We evaluated the leaf attributes (concentration of N, P analysis of leaf mass per unit area and N:P ratio) and colonization of roots by micorrhiza. Chemucally, the soil of the rupestrian fields shows very low availability of P and N, comparable to other soils OCBILs (Western Australia and the Cape region in South Africa). The proportion of root colonization of plants by fungi was positively correlated with [P] soil of each community, supporting the Lambers model's on a regional scale. Species of rupestrian fields have a high N:P ratio leaf (average of 43 for all fields studied), higher than those found in other OCBILs, suggesting a strong limitation by phosphorus. We found average 183 gm.m-2 for LMA, 12,46 mg.g-1 and 0,36 mg.g-1 to P leaf. The results obtained suggests that rupestrian fields should also be classified as OCBILs, status wich would require greater attention to these fields still little studied and already threatened ecologicaly / Mestrado / Ecologia / Mestre em Ecologia

Global environmental changes, especially land-use changes, have profound effects on both ecosystem functioning and biodiversity, having already altered many ecosystem services. These losses emphasize the need to preserve what remains; however when conservation programs are not sufficient, restoring areas that have been destroyed or disturbed can improve conservation efforts and mitigate damages. This work focuses on campos rupestres, Neotropical grasslands found at altitudes, which are part of the Cerrado (Brazilian savannas). They host a great biodiversity with a high level of endemism and, like other mountain ecosystems, provide valuable ecosystem services, such as water purification and recreational services. They have been and still are being impacted by human activities, such as civil engineering construction, quarrying or mining. The first objective of this thesis was to describe the reference ecosystem in order to aim for a clear restoration target and to monitor progress and success. We show that campos rupestres are composed of at least two distinct plant communities (i.e. sandy and stony grasslands), each having a specific composition and structure, hosting a great biodiversity. Several phenological patterns occur among the herbaceous communities: the majority of species flowers and fruits appear during the rainy season but other patterns can be observed. During our 2-year survey, some dominant species belonging to Poaceae, among others, were not observed reproducing, which implies limited chances to disperse on degraded areas. Campo rupestre vegetation is not resilient following a strong disturbance: several years after the disturbance, almost no native species are encountered on the degraded areas, soils are completely altered and seed bank recomposes only with non-target ruderal species. According to the filter model, a local community is a subset of the regional species pool determined by a set of dispersal, abiotic and biotic filters. Acting on the different filters to influence the plant community was the core of our restoration interventions. We then applied three in-situ restoration protocols (hay transfer, species translocation and turf translocation) to restore both kinds of grassland. Hay transfer does not allow the restoration of campo rupestre vegetation because of soil alteration and mainly because of poor seed quality. Indeed, germination studies show that, while some Xyridaceae and Velloziaceae have a high germinability, some dominant Poaceae, Cyperaceae or Asteraceae species have embryoless, unviable or dormant seeds, which makes seeding less efficient. There is no evidence that fire-related cues enhance germination in campos rupestres. Species translocation is successful for only one species, Paspalum erianthum; for the others, root damages probably impede survival. Finally, turf translocation is the most successful method, since numerous species are re-introduced on degraded areas. However due to the low resilience of pristine campos rupestres where turfs are taken from, turf translocation can only be considered in the case of habitat rescue, in circumstances when complete habitat destruction is otherwise unavoidable. Face to the difficulty to restore these peculiar grasslands, the protection and the conservation of campos rupestres must be made a high priority

Les changements globaux, notamment les changements d'usage des terres, modifient profondément le fonctionnement des écosystèmes et la biodiversité et, ont déjà impacté de nombreux services écosystémiques. La disparition de ces écosystèmes souligne la nécessité de préserver les zones intactes, et la restauration des zones détruites ou perturbées peut permettre de venir en appui aux efforts de conservation et minimiser les dommages. Ce travail a pour objet d’étude les campos rupestres, des pelouses néotropicales d’altitude, faisant partie du Cerrado (savane brésilienne), qui recèlent une importante biodiversité et qui, comme bien d'autres écosystèmes de montagne, fournissent de précieux services écosystémiques tels que la filtration de l’eau. Ils ont été, et sont encore, grandement affectés par les activités humaines (les travaux de génie civil, les carrières ou les mines). Le premier objectif de cette thèse était de décrire l'écosystème de référence, afin de définir clairement un objectif de restauration et mesurer les progrès et le succès de la restauration. Nous avons montré que les campos rupestres sont composés d'au moins deux communautés végétales distinctes (une avec un substrat caillouteux et l’autre avec un substrat sableux), chacune ayant une composition en espèces et une structure particulières ainsi qu’une grande biodiversité. La phénologie reproductive varie au sein des communautés herbacées: la majorité des espèces fleurissent et fructifient pendant la saison des pluies, d'autres se reproduisent en revanche durant la saison sèche. Tout au long de nos 2 années de suivis phénologiques, certaines espèces dominantes, notamment des Poaceae, n'ont pas été observées en fleur impliquant une dispersion limitée de ces espèces vers les zones dégradées. Les communautés végétales de campos rupestres ne sont pas résilientes aux fortes perturbations: plusieurs années après, presque aucune des espèces cibles n’ont été trouvées en zones dégradées, les sols ont complètement été modifiés et les banques de graines ne se sont recomposées qu’avec des espèces rudérales non désirées. Selon le modèle des filtres, une communauté résulte d’un pool régional d’espèce sélectionné par un ensemble de filtres : de dispersion, abiotique et biotique. Les interventions de restauration mises en place avaient pour but d’agir sur ces différents filtres afin de diriger la dynamique des communautés végétales. Nous avons mis en place trois protocoles de restauration in-situ (le transfert de foin, la translocation d’espèce et la translocation de plaque de végétation) pour restaurer les deux types de communautés de campos rupestres identifiées. Le transfert de foin n’a pas permis la restauration des communautés végétales de campos rupestres en raison de l’importante altération des sols et, surtout, à cause de la mauvaise qualité des graines. En effet, nos études de germination ont montré que, alors que certaines espèces de Xyridaceae et Velloziaceae germent très bien, certaines espèces dominantes de Poaceae, de Cyperaceae ou d’Asteraceae ont des graines soit vides, soit non viables, soit dormantes ; le semis se révèle alors peu efficace. Nous n’avons pas mis en évidence d’effet positif du feu sur la germination des espèces de campos rupestres. La translocation d'espèces s’avère un succès pour une seule espèce, Paspalum erianthum. Pour les autres, les dommages causés au niveau des racines lors de la translocation limitent probablement leur survie. Enfin la translocation de plaques de végétation s’avère être la méthode la plus efficace permettant à de nombreuses espèces d’être réintroduites en zones dégradées. En raison de la faible résilience des campos rupestres dans lesquels les plaques de végétation ont été prélevées, cette méthode ne peut être envisagée que pour sauver des habitats dans le cas extrême où leur destruction est inévitable. Face à la difficulté de restaurer les campos rupestres, leur protection et leur conservation doit être une priorité / Global environmental changes, especially land-use changes, have profound effects on both ecosystem functioning and biodiversity, having already altered many ecosystem services. These losses emphasize the need to preserve what remains; however when conservation programs are not sufficient, restoring areas that have been destroyed or disturbed can improve conservation efforts and mitigate damages. This work focuses on campos rupestres, Neotropical grasslands found at altitudes, which are part of the Cerrado (Brazilian savannas). They host a great biodiversity with a high level of endemism and, like other mountain ecosystems, provide valuable ecosystem services, such as water purification and recreational services. They have been and still are being impacted by human activities, such as civil engineering construction, quarrying or mining. The first objective of this thesis was to describe the reference ecosystem in order to aim for a clear restoration target and to monitor progress and success. We show that campos rupestres are composed of at least two distinct plant communities (i.e. sandy and stony grasslands), each having a specific composition and structure, hosting a great biodiversity. Several phenological patterns occur among the herbaceous communities: the majority of species flowers and fruits appear during the rainy season but other patterns can be observed. During our 2-year survey, some dominant species belonging to Poaceae, among others, were not observed reproducing, which implies limited chances to disperse on degraded areas. Campo rupestre vegetation is not resilient following a strong disturbance: several years after the disturbance, almost no native species are encountered on the degraded areas, soils are completely altered and seed bank recomposes only with non-target ruderal species. According to the filter model, a local community is a subset of the regional species pool determined by a set of dispersal, abiotic and biotic filters. Acting on the different filters to influence the plant community was the core of our restoration interventions. We then applied three in-situ restoration protocols (hay transfer, species translocation and turf translocation) to restore both kinds of grassland. Hay transfer does not allow the restoration of campo rupestre vegetation because of soil alteration and mainly because of poor seed quality. Indeed, germination studies show that, while some Xyridaceae and Velloziaceae have a high germinability, some dominant Poaceae, Cyperaceae or Asteraceae species have embryoless, unviable or dormant seeds, which makes seeding less efficient. There is no evidence that fire-related cues enhance germination in campos rupestres. Species translocation is successful for only one species, Paspalum erianthum; for the others, root damages probably impede survival. Finally, turf translocation is the most successful method, since numerous species are re-introduced on degraded areas. However due to the low resilience of pristine campos rupestres where turfs are taken from, turf translocation can only be considered in the case of habitat rescue, in circumstances when complete habitat destruction is otherwise unavoidable. Face to the difficulty to restore these peculiar grasslands, the protection and the conservation of campos rupestres must be made a high priority