Since they appeared, wireless technologies have deeply transformed our society. Today, wireless internet access and other wireless applications demandincreasingly more traffic. However, the continuous traffic increase can be unbearableand requires rethinking and redesigning the wireless technologies inmany different aspects. Aiming to respond to the increasing needs of wirelesstraffic, we are witnessing a rapidly evolving wireless technology scenario.This thesis addresses various aspects of the transmitters used in wireless communications.Transmitters present several hardware (HW) impairments thatcreate distortions, polluting the radio spectrum and decreasing the achievabletraffic in the network. Digital platforms are now flexible, robust and cheapenough to enable compensation of HW impairments at the digital base-bandsignal. This has been coined as ’dirty radio’. Dirty radio is expected in future transmitters where HW impairments may arise to reduce transmitter cost or to enhance power efficiency. This thesis covers the software (SW) compensation schemes of dirty radio developed for wireless transmitters. As describedin the thesis, these schemes can be further enhanced with knowledge of thespecific signal transmission or scenarios, e.g., developing cognitive digital compensationschemes. This can be valuable in today’s rapidly evolving scenarioswhere multiple signals may co-exist, sharing the resources at the same radiofrequency (RF) front-end. In the first part, this thesis focuses on the instrumentation challenges andHWimpairments encountered at the transmitter. A synthetic instrument (SI)that performs network analysis is designed to suit the instrumentation needs.Furthermore, how to perform nonlinear network analysis using the developedinstrument is discussed. Two transmitter HW impairments are studied: themeasurement noise and the load impedance mismatch at the transmitter, asis their coupling with the state-of-the-art digital compensation techniques.These two studied impairments are inherent to measurement systems and areexpected in future wireless transmitters. In the second part, the thesis surveys the area of behavioral modeling and digital compensation techniques for wireless transmitters. Emphasis is placed on low computational complexity techniques. The low complexity is motivated by a predicted increase in the number of transmitters deployed in the network, from base stations (BS), access points and hand-held devices. A modeling methodology is developed that allows modeling transmitters to achieve both reduced computational complexity and low modeling error. Finally, the thesis discusses the emerging architectures of multi-channel transmittersand describes their digital compensation techniques. It revises the MIMOVolterra series formulation to address the general modeling problem anddrafts possible solutions to tackle its dimensionality. In the framework of multi-channel transmitters, a technique to compensate nonlinear multi-carrier satellite transponders is presented. This technique is cognitive because it uses the frequency link planning and the pulse-shaping filters of the individual carriers. This technique shows enhanced compensation ability at reduced computational complexity compared to the state-of-the-art techniques and enables the efficient operation of satellite transponders. / <p>QC 20150526</p>

The structure of direct-current microdischarges is investigated using a detailed two-dimensional multi-species continuum model. Microdischarges are directcurrent discharges that operate at a relatively high pressure of about 100 Torr and geometric dimensions in the 10-100 micrometer range. Our motivation for the study of microdischarges comes from a potential application of these devices in microthrusters for small satellite propulsion. The Micro Plasma Thruster (MPT) concept consists of a direct-current microdischarge in a geometry comprising a constant area flow section followed by a diverging exit nozzle. A detailed description of the plasma dynamics inside the MPT including power deposition, ionization, coupling of the plasma phenomena with high-speed flow, and propulsion system performance is reported in this study. A two-dimensional model is developed as part of this study. The model consists of a plasma module coupled to a flow module and is solved on a hybrid unstructured mesh framework. The plasma module provides a self-consistent, multispecies, multi-temperature description of the microdischarge phenomena while the flow module provides a description of the low Reynolds number compressible flow through the system. The plasma module solves conservation equations for plasma species continuity and electron energy, and Poisson’s equation for the self-consistent electric field. The flow module solves mass, bulk gas momentum and energy equations. The coupling of energy from the electrostatic field to the plasma species is modeled by the Joule heating term which appears in the electron and heavy species energy equations. Discretization of the Joule heating term on unstructured meshes requires special attention. We propose a new robust method for the numerical discretization of the Joule heating term on such meshes using a cell-centered, finite volume approach. A prototypical microhollow cathode discharge (MHCD) is studied to guide and validate the modeling effort for theMPT. Computational results for the impedance characteristics as well as electrodynamic and chemical features of the discharge are reported and compared to experimental results. At low current (< 0.1 mA), the plasma activity is localized inside the cylindrical hollow region of the discharge operating in the so-called “abnormal regime”. For larger currents, the discharge expands over the outer flat surface of the cathode and operates in the “normal regime”. Transient relaxation oscillations are predicted in the plasma properties for intermediate discharge currents ranging from 0.1 mA to 0.3 mA; a phenomenon that is reported in experiments. The MPT, in its present configuration, is found to operate as an electrothermal, rather than as an electrostatic thruster. A significant increase in specific impulse, compared to the cold gas micronozzle, is obtained from the power deposition into the expanding gas. For a discharge voltage of 750 V, a power input of 650 mW, and an argon mass flow rate of 5 sccm, the specific impulse of the device is increased by a factor of 1.5 to a value of 74 s. The microdischarge remains mostly confined inside the micronozzle and operates in an abnormal regime. Gas heating, primarily due to ion Joule heating, is found to have a strong influence on the overall discharge behavior. The study provides crucial understanding to aid in the design of direct-current microdischarge based thrusters. / text

Satellite cells are adult stem cells that contribute to hypertrophy and repair in muscles. It is hypothesized that in muscular dystrophy, the satellite cells population is depleted at a very early age, due to repeated muscle damage and repair. Satellite cell transplantation is a potentially useful therapy for muscle diseases, but the lack of an efficient delivery system has hindered its application. The presented work focuses on two specific aims that address the need for more effective cell delivery methods for cell-based therapy. In Specific Aim 1 enhanced tissue culture techniques, such as heat stress, are used to increase cell survival in satellite cell transplantation studies. Also addressed within this specific aim are methods to label and evaluate performance using real-time PCR techniques.Although much work remains to enhancing the viability of in vitro expanded myoblasts derived from satellite cells, a second important hurdle is the systemic delivery of satellite cells to multiple sites (all muscles, in the case of muscular dystrophies). In vitro and in vivo experiments are being undertaken to explore the physiological role of cell signaling systems involved in directed migration and to determine if these chemokine and growth factors can be manipulated to enhance efficacy of cell-based therapies involving skeletal muscle satellite cells. Specific Aim 2 addresses migration of satellite cells to sites of injury and methods to track transplanted cells within the host. Presented here is the use of FAST SPECT II imaging of 111-Indium oxine radiolabeled satellite cells. The long lifetime of 111-indium oxine and the ability to quantify label using FAST SPECT imaging techniques make this technique ideal for in-vivo tracking of transplanted satellite cells for week long studies. Without in-vivo imaging techniques cell fate studies require sequential animal sacrifice with histological sectioning. This not only increases the number of animals used but also adds a significant inter-animal variability to their assessment. The determination of cell fate after transplantation will have a major impact on cell therapy for treatment of muscle disease as well as other stem cell therapies.

Skeletal muscle regeneration is a multifaceted process requiring the spatial and temporal coordination of myogenesis as well as angiogenesis. While these processes are often studied independently, recent evidence from our lab has shown that the resident adult stem cell population within skeletal muscle, called satellite cells, begins secreting soluble growth factors likely to contribute to the proangiogenic response. The overall aim of this study is to investigate the role of pro-angiogenic factors secreted by satellite cells during skeletal muscle regeneration. Results from the study indicate that Hepatocyte Growth Factor (HGF) is a critical protein for the proangiogenic effect of satellite cells. It was also shown that in hypoxic environments, such as those seen in an injury state, it appears that satellite cells decrease their proangiogenic effect if oxygen levels fall below a threshold level. This decrease in pro-angiogenic effect in the hypoxic environment appears to be due to the decrease in HGF expression and protein secretion and is not compensated for by the increase in Vascular Endothelial Growth Factor secretion also seen in the hypoxic response. Furthermore, the regulation of HGF in these hypoxic conditions appears to be in part due to increased levels of hypoxia inducible factor, which are acting on the hypoxia response element site found on the HGF promoter. In the last set of experiments, this injury response was further investigated as the effect of satellite cell mediated angiogenesis was examined in the disease state of muscular dystrophy. Here, we also observed a reduction in angiogenesis from media conditioned by satellite cells from dystrophic muscle compared to healthy muscle. Overall, this study further strengthens the case for satellite cells as important mediators of the angiogenic response in regenerating muscle and may serve as a potential site for therapeutic intervention in the future.

Skeletal muscle regeneration is dependent upon the influences of intrinsic and extrinsic factors that stimulate satellite cells. Regenerating proteins are upregulated at the onset of trauma or inflammation in the pancreas, gastrointestinal tract, liver, neural cells and other tissues. Studies have shown that Reg proteins have a mitogenic, anti-apoptotic and anti-inflammatory function in damaged tissues and is necessary for normal progression of regeneration. As skeletal muscle is also able to regenerate itself at a rapid rate, it seems highly likely that Reg proteins function to promote myogenesis in skeletal muscle regeneration. Therefore, the goal of our research was to characterize the expression of the Reg proteins and receptor in regenerating skeletal muscle and satellite cells, investigate the effect of exogenous Reg protein on myogenesis, and to examine direct Reg protein effect on satellite cell activity. To determine whether Reg proteins participate in skeletal muscle regeneration, mice were injected with marcaine in their tibialis anterior muscles to induce skeletal muscle damage. The gene expression analysis of undamaged and marcaine-damaged tibialis anterior muscles and mice satellite cells showed that Reg I, II, IIIα, IIIγ, IV and EXTL3 genes are present during skeletal muscle regeneration and satellite cells significantly express Reg I, IIIα, IIIγ and EXTL3. As Reg I and IIIα are most prevalent in vivo and in vitro respectively, we advocate these isoforms as the predominant candidates in skeletal muscle regeneration. To determine the effect of exogenous Reg protein on myogenesis, we performed gene expression and muscle morphometry analysis of Reg IIIα or PBS injected tibialis anterior muscles. Interestingly, our results indicate that the addition of Reg IIIα to damaged muscles inhibited myogenesis. To determine the direct effect of Reg protein on myogenic stem cell activity, Reg proteins were added to mice satellite cells and C2C12 cells. Results from these studies were inconclusive due to the failure of known positive and negative controls. Overall, our studies suggest that Reg proteins contribute to skeletal muscle regeneration; however, as an overabundance of Reg IIIα in regenerating tissues may have inhibited myogenesis, it is imperative that other isoforms or lower concentrations be investigated.

Young female rats treated with trenbolone demonstrated an increase in weight gain per day and overall weight increase during the treatment period. Trenbolone treated rats also experienced improved feed efficiency. Muscles removed from the lower hind limb of trenbolone treated rats had a greater DNA to protein ratio than muscles from control animals. However, there was no significant difference in wet muscle weight between trenbolone treated and control muscles. Satellite cells from untreated female rats were not responsive to trenbolone added in vitro. In studies utilizing serum free medium, trenbolone alone, and in the presence of growth factors, could not stimulate proliferation above controls. In similar serum free medium studies, satellite cells from trenbolone treated rats were more responsive to growth factors than cells from control rats.

The Atmospheric Chemistry Experiment (ACE) is a satellite mission for remote sensing of the Earth's atmosphere using the solar occultation technique. The primary instrument on this satellite is the Atmospheric Chemistry Experiment Fourier Transform Spectrometer (ACE-FTS). ACE-FTS retrievals are described with a focus on the creation of <em>a priori</em> temperature and pressure profiles. ACE-FTS measurements are then used to investigate the chemistry, transport and trends of chlorine, fluorine and water in the stratosphere, leading to an improved understanding of processes affecting both stratospheric ozone depletion and global climate change. <br /><br /> Total chlorine (Cl_TOT) in the stratosphere is determined using ACE-FTS measurements of eleven chlorine-containing species, supplemented by both other measurements and models, to determine Cl_TOT as a function of altitude in five latitude zones. All resulting Cl_TOT profiles are nearly linear, with a slight slope. Mean Cl_TOT for 2004 is determined to be 3. 65 ppbv for both the northern and southern midlatitudes (with a precision and estimated accuracy of ??0. 09 and ??0. 13 ppbv, respectively). A slightly lower value of mean Cl_TOT is determined for the tropics and slightly higher values at high latitudes. Total fluorine (F_TOT) in the stratosphere is also determined primarily from ACE-FTS measurements using a similar approach, resulting in stratospheric F_TOT profiles which are nearly linear with mean values ranging from 2. 50 to 2. 59 ppbv for each latitude zone (with a precision of 0. 04-0. 07 ppbv and an estimated accuracy of 0. 15 ppbv). The observed slopes and pattern of latitudinal variation are evidence of the beginning of a decline in global stratospheric chlorine and of the continuing increase in global stratospheric fluorine levels. <br /><br /> The abundance of water in the stratosphere is investigated for the northern hemisphere midlatitudes in 2004 using ACE-FTS measurements. Potential water is determined as [H₂O]+2[CH₄] and from [H₂O] versus [CH₄] correlations, resulting in a value of 7. 14??0. 05 ppmv, which is used to determine a value of 3. 65??0. 15 ppmv for the mean abundance of water entering the stratosphere. Both values are compared directly with historical data from the Atmospheric Trace Molecule Spectroscopy (ATMOS) instrument (1985-1994) and show a negligible change, implying that the increases observed by ATMOS and other long-term measurements from that time period have not continued. <br /><br /> The removal of stratospheric water in the Arctic vortex is investigated using ACE-FTS measurements. Using derived quantities from a meteorological data assimilation, northern hemisphere occultations from early 2004 are classified as vortex, vortex edge or extravortex. [CH₄] versus [N₂O] correlations are used to further classify the extravortex occultations as tropical, subtropical or midlatitude. Comparisons between profiles of [N₂O], [CH₄] and [H₂O] inside and outside the Arctic vortex, give estimates of upper stratospheric and lower mesospheric descent rates, indicating that descent in the winter 2004 Arctic vortex was rapid, with evidence of descent at higher altitudes than in past years. <br /><br /> The dehydration of air in the tropical tropopause layer and mechanisms for the entry of water vapor into the stratosphere are investigated by an analysis of ACE-FTS profiles of temperature, water vapor and [HDO]/[H₂O]. Month-to-month comparisons for 2004 and 2005 reveal a clear pattern of seasonal variation and a correlation between minimum temperature and maximum HDO depletion. Further interpretation indicates that the gradual dehydration mechanism accompanied by lofting of ice particles in the tropical troposphere is the most likely explanation for the observed seasonal variation and the shape of the [HDO]/[H₂O] profiles.

The Earth’s magnetic field is generated by fluid motion of liquid iron in the outer core. Flows at the top of the outer core are believed to be responsible for the secular variation (SV) observed at the surface of the Earth. Modelling of this flow is open to considerable ambiguity, though methods adopting different physical assumptions do lead to similar flow velocity regimes. Some aspects of the ambiguities are investigated in this thesis. The last decade has seen a significant improvement in the capability to observe the global field at high spatial resolution. Several satellite missions have been launched, providing a rich new set of scalar and vector magnetic measurements from which to model the global field in detail. These data complement the existing record of groundbased observatories, which have continuous temporal coverage at a single point. I exploit these new data to model the secular variation (SV) globally and attempt to improve the core flow models that have been constructed to date. Using the approach developed by Mandea and Olsen (2006) I create a set of evenly distributed ‘Virtual Observatories’ (VO), at 400km above the Earth’s surface, encompassing satellite measurements from the CHAMP satellite over seven years (2001-2007), inverting the SV calculated at each VO to infer flow along the core-mantle boundary. Direct comparison of the SV generated by the flow model to the SV at individual VO can be made. Thus, the residual differences can be investigated in detail. Comparisons of residuals from flow models generated from a number of VO datasets provide evidence that they are consistent with internal and external field effects in the satellite data. I also show that the binning and processing of the VO data can induce artefacts, including sectorial banding, into the residuals. By employing the core flows from the inversion of SV data it may be possible to forecast the change of the present magnetic field (as measured) forwards in time for a short time period (e.g. less than five years) within an acceptable error budget. Using simple advection of steady or non-steady flows to forecast magnetic field change gives reasonably good fit to field models such as GRIMM, POMME or xCHAOS (< 50nT root mean square difference after five years). The forecast of the magnetic field change can be improved by optimally assimilating measurements of the field into the forecast from flow models at discrete points in time (e.g. annually). To achieve this, an Ensemble Kalman Filter (EnKF) can be used to the capture non-linearity of the model and delineate the error bounds by means of a Monte Carlo representation of the field evolution over time. In the EnKF model, an ensemble of probable state vectors (Gauss coefficients) evolve over time, driven by SV derived from core flows. The SV is randomly perturbed at each step before addition to the state vectors. The mean of the ensemble is chosen as the most likely state (i.e. field model) and the error associated with the estimate can be gauged from the standard deviation from the mean. I show an implementation of the EnKF for steady and non-steady flows generated from ‘Virtual Observatory’ field models, compared to the field models GRIMM and xCHAOS over the period 2002–2008. Using the EnKF, the maximum difference never exceeds 25nT over the period. This promising approach allows measurements to be included into model predictions to improve the forecast.

Marine turtles are an ancient group of reptiles that have been used by humans as a source of protein for over 7,000 years. In recent decades, acknowledgement of the various threats to marine turtles, including the deleterious impact of historical and contemporary use on many populations, led the International Union for the Conservation of Nature (IUCN) to list all seven extant species of marine turtle on their Red List of Threatened Species. Consequently, marine turtles are often given protected status in the national legislation of countries around the world, despite the existence of ongoing use cultures in communities that live with marine turtles. Conservation strategies are challenged by the migratory nature of marine turtles, which have complex life histories typically involving the use of habitats in the jurisdictions of multiple sovereign states as well as the high seas. As a result, a suite of multi-lateral environmental agreements (MEAs) list marine turtles in the most highly protective categories. Thus, governments of sovereign states that have acceded to the various MEAs are committed to conservation strategies requiring national action and cooperative multi-lateral action, which can conflict with interests of communities with a tradition of marine turtle consumption. In this thesis I provide examples of how contemporary scientific research methods can elucidate the migratory behaviours of marine turtles, and can help define range of populations subject to national conservation action and use. I examine specific examples of how this information can inform national and multi-lateral conservation policies and strategies; how those policies and strategies interact and impact on traditional cultures of marine turtle use in the UK Overseas Territories in the Caribbean; and provide an example of the potential benefits of engaging stakeholders with contemporary research methods. This thesis highlights the utility of a multi-disciplinary approach to research underpinning marine turtle conservation and management, which acknowledges the limitations of MEAs and national government capacity, and which incorporates participation of those communities engaged in marine turtle consumption.

International Telemetering Conference Proceedings / October 17-20, 1994 / Town & Country Hotel and Conference Center, San Diego, California / A comprehensive examination of the market demands for cost reduced satellite telemetry & control stations will be presented. These systems are implemented using flexible, open architecture-based high performance real-time systems. The trend for combining telemetry monitoring of satellite data with closed-loop satellite command and control functions will be presented. This combined functionality opens up the possibilities for completely integrated, reduced cost satellite control systems. The market forces driving the demand for this integrated functionality include the broadening of non-military satellite applications, the widening international deployment of commercial satellites and the accompanying drive toward decentralized satellite control. The major requirements for the telemetry processing and command & control functionality of the integrated, reduced cost satellite control system will be presented. These requirements include: full real-time performance for processing telemetry data; flexible architecture for the incorporation of a wide range of I/O devices; capability of performing real-time, closed-loop control based on conditions in the telemetry data; user friendly development environments for application-specific customization of the system; and low system costs with the capability of indigenous support. The divergent requirements of performance, flexibility and price of these integrated, reduced cost satellite control systems is made possible via the use of open architecture building blocks that include standard VME boards combined with specialized real-time software drivers and user oriented, flexible Graphical User Interface (GUI) software.