Proefschrift Universiteit van Amsterdam. / Met lit. opg. - Met samenvatting in het Nederlands.

Thesis (Ph. D.)--Ohio State University, 2004. / Title from first page of PDF file. Document formatted into pages; contains xviii, 133 p.; also includes graphics (some col.). Includes bibliographical references (p. 121-133). Available online via OhioLINK's ETD Center

Thesis (Ph. D.)--University of Wisconsin--Madison, 1940. / Typescript. Includes abstract and vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 82-84).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1960. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 243-284).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1962. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1965. / Typescript. Vita. "Some biological activities of papain-produced subunits of human 7S gamma globulins" [by] H.F. Deutsch, N.O. Thorpe and H.H. Fudenberg, reprinted from Immunology, vol. 6, no. 6, November 1963, pp. 539-550, inserted between leaves 266 and 267. Description based on print version record. Includes bibliographical references (leaves 267-276).

The protozoan blood-borne parasite Trypanosoma cruzi is the causative agent of Chagas' disease, an enervating and often fatal illness prevalent in South and Central America for which there is no effective treatment. T. cruzi has a cell-surface trans- sialidase which transfers sialic acid from mammalian oligosaccharides to the parasite. This action allows adhesion to and invasion of mammalian cells, subsequently allowing parasitic replication. This protein therefore is exploitable and represents a potential target for the development of chemotherapeutic agents. This thesis describes the purification of recombinant trans-sialidase and the development of a rapid, reliable spectrophotometric coupled assay to measure trans-sialidase activity. It also details the use of three mutually exclusive synthetic oligosaccharide libraries to map substrate recognition for the enzyme. Synthetic fragments of the natural branched oligosaccharide substrates have also been sialylated on a preparative scale, demonstrating the use of trans-sialidase in synthetic oligosaccharide chemistry.

This thesis describes in full the development of a biological method of estimating cholecystokinin (CCK) in human serum by superfusion of rabbit gall-bladder strips, from the early stages of the manual technique, which would estimate approximately twelve samples per day, to the more sophisticated and clinically useful automated technique of today which can estimate up to forty eight sample solutions in duplicate each day. In developing this bioassay for CCK it was necessary to determine whether the gall-bladder preparation was stable for the duration of the experiment, and also whether or not there were other factors influencing the response of the gallbladder strips to a given stimulus. It was also necessary to determine whether the assay was entirely specific for CCK in human serum and to counteract any possible degradation of CCK during processing and storage of serum. In the light of the findings during the above experiments the technique was modified to eliminate errors that would otherwise be made in estimating the CCK content of a serum sample. Since our attempts to set up a radioimmunoassay were unsuccessful a comparison of this bioassay was made with another groups' radioimmunoassay. Although no direct comparison could be made as the two assays measure CCK in totally different units, nevertheless a straight line relationship between the two very different methods was found, and this experiment led directly to the discovery by bioassay of CCK mimicking substances in serum. The action of trypsin on CCK has been clearly demonstrated and its ability to release the C-terminal octa and more probably the dodeca peptide, both of which are considerably more active than the full molecule, is beyond question. It is therefore important that an enzyme inhibitor is added to blood samples to prevent this spontaneous breakdown of more active fragments and prevent false readings of serum cholecystokinin activity. During chromatographic investigations a new molecule in human serum which possessed cholecystokinetic activity but had a molecular weight in excess of 30,000 (c/f 3,900 for normal CCK) was discovered. This molecule appeared to predominate in the fasting state but to decrease in concentration during the response to a meal. The practical use of measuring serum CCK is demonstrated in some clinical trials in which CCK's target organs are removed or the stimulation for its release is modified by various surgical procedures. Some interesting changes are noted.

The literature on biochemical changes in open cardiac surgery with especial reference to plasma protein and amino acids has been reviewed. The changes that take place in plasma proteins during oxygenation of blood of open cardiac surgery have been investigated with emphasis on denaturation. This phenomena has been examined by the assay of blood and plasma sulfhydryl groups by different reagents and also viscosity and turbidity changes. The limitations of the two latter investigations has been discussed. Haemolytic changes in plasma have been examined and the related importance of sulfhydryl groups discussed. Plasma protein changes have been investigated by methods depending on net charge, shape, size, and molecular weight of individual molecules. Although adrenal cortical response to open cardiac surgery has been investigated it has been pertinent to reinvestigate adrenal cortical secretion pari passu with amino acid data from blood and urine samples of patients not only because of their metabolic associations, but also because of the opportunity of comparing surgical patients receiving supplementary haydrocortisone throughout open cardiac surgery with those not supplemented - the latter therefore depending on their own intrinsic secretion during surgical stress. A review of relevant literature has accordingly been presented with emphasis on the difficulties involved in ACTH assay of plasma from surgical patients, and the practical advantages of 17-hydroxycorticostereid assay in lieu of ACTH assays. The plasma and urinary amino acid increase have been examined together in detail comparing not only the increase in each amino acid at each operation but also comparing the increase of an amino acid in all the operations investigated thus obtaining mean values of each amino acid at regular intervals of time throughout each perfusion.