The principle aim of this study was to gain an understanding of the conditions and processes governing the occurrence of simultaneous nitrification and denitrification (SND). SND is the process that combines nitrification and denitrification in the same reactor (at the same time) under fully aerobic conditions. From various studies, two main hypotheses, one physical and one biological, have been proposed to explain SND (Simultaneous Nitrification and Denitrification). Significant research has been performed on the biological aspects, whereas relatively little is known about the physical explanation. Therefore, further investigations of physical explanation on SND (Simultaneous Nitrification and Denitrification) are carried out in this thesis. To fulfill this principal objective, two major tasks were preformed: experimental studies and model development. The experimental investigation was conducted using lab scale sequencing batch reactors (SBR). The operating conditions of the reactors were varied corresponding to the aim of each experiment. The influent wastewater was collected from the effluent of an anaerobic pond at an abattoir wastewater treatment plant. The main experimental studies focused on three factors, the effect of soluble organic carbon, floc size and dissolved oxygen (DO) concentrations, on the SND activity. The results revealed that all these factors had a significant influence on the degree of SND achieved. Almost 50% of inorganic nitrogen lost by SND (Simultaneous Nitrification and Denitrification) could be achieved when operating at a soluble COD:TKN ratio of 6. A dramatic increase in SND activity to 85% was found when this ratio reached 10. With a soluble COD:TKN ratio of 15, complete nitrogen removal by SND could be achieved. The effect of dissolved oxygen (DO) was equally strong. SND could completely occur at very low DO concentrations (0.2 mg/L). However, the nitrogen removal in this range was substantially limited by the low nitrification rate. To improve the nitrification rate but still achieve effective denitrification, a DO concentration of around 0.4-0.5 mg/L seems to be an optimal value to maintain a significant degree of SND. In this range, the nitrification rate reached 50% of the rate found at DO of 1.1 mg/L and 60% SND activity was achieved. The effect of bacterial floc size on SND was also quite remarkable. It was found that an SBR operating with a median floc size of 80 mm could achieve 80% SND, whereas the SND activity decreased to only 50% after the median floc size was reduced to 40 mm in the following treatment cycle. A complete nitrogen balance over the whole process was performed to confirm the occurrence of SND in such systems. Under typical operating conditions, it was found that the nitrogen gas was the major nitrogen product of the treatment process (approximately 58% of the total output). 14% of nitrogen was assimilated to biomass whereas 23 % of nitrogen at the end of the process was in the soluble form (organic nitrogen, nitrite, nitrate and ammonium). The mathematical dynamic model was developed to gain a better understanding of SND in the situation that is difficult to investigate experimentally. The overall model structure can be divided into 4 main areas : 1. a ‘micro’ level model for a single floc 2. the reaction rates for a single floc size 3. the reaction rates for the entire reactor considering the floc size distribution 4. a ‘macro’ model for the whole reactor including the operational changes throughout the cycle. It was found that the model can predict the SND behavior well for the system operating under typical influent characteristics (SCOD:TKN of 10). However, poor predictions were found at different levels of SCOD:TKN. Two crucial reasons can be given. Firstly, this model did not include intracellular carbon storage by bacteria. Secondly, many parameters, especially floc and microorganism characteristics (i.e. intra floc biomass distribution, growth and decay of the microorganism, etc.) could not be determined or estimated accurately. However, under normal operating conditions of this study, the model advances the fundamental understanding of SND process in activated sludge system. The simulation results showed that both floc diameter and liquid phase concentration are important factors influencing the internal floc concentrations. It was also predicted that an anoxic microzone, caused by oxygen diffusion limitation, potentially occurs in the floc center. This microzone therefore enhances denitrification activity inside the floc. A number of major conclusions can be drawn from this thesis: 1. SND potentially occurs as a result of physical phenomenon 2. high soluble COD is beneficial to SND activity 3. suitable floc size distribution (with more large flocs) can enhance SND 4. major nitrogenous product of the treatment process is nitrogen gas 5. dissolved oxygen optimization is critical to get good nitrification rate and SND.

Membrane compartmentalisation allows eukaryotic cells to perform complex processes by combining dedicated sets of proteins in the same organelle. To achieve this, the cell must first target the appropriate proteins, primarily synthesised on cytosolic ribosomes, to the correct subcellular location. Components of the secretory pathway/endomembrane system begin this journey via their signal sequence-dependent delivery to the endoplasmic reticulum (ER). These ER targeting signals are hydrophobic, and typically function whilst the protein is being synthesised, via a so-called 'co-translational' pathway. However, some hydrophobic signals can also facilitate post-translational protein targeting to the ER, or initiate regulated protein degradation in the cytosol. Tail-anchored (TA) proteins are transmembrane proteins with a single C-terminal transmembrane domain that functions as both their subcellular targeting signal and membrane anchor. Recent evidence suggests that the canonical TRC40 pathway, through which mammalian TA proteins are delivered to the ER, may not be essential in vivo. In this thesis, I provide functional evidence for the existence of an orthologous SRP-independent (SND) pathway in mammalian cells and identify roles for both the signal recognition particle (SRP)-mediated pathway and presumptive mammalian SND pathway in the biogenesis of TA proteins. I conclude that although TRC40 normally plays a role in TA protein biogenesis, it is not essential, and speculate that these alternative pathways make a significant contribution to the apparent redundancy of the TRC40 pathway in vivo. The soluble components that act upstream of TRC40 during protein biogenesis also play an important role in the recognition and selective degradation of hydrophobic membrane and secretory proteins that mislocalise to the cytosol. I now provide preliminary evidence that TRC40 appears to exhibit dual functionality, having a non-essential role in TA protein delivery, whilst also contributing to protein quality control by acting as a putative holdase. My data suggest that both TRC40 and BAG6 can influence the proteasomal degradation of a novel class of substrates, which I have termed the aberrant short secretory proteins.

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No. of bitstreams: 1 chaucanes_pam_me_ilha.pdf: 4074329 bytes, checksum: b4e87799b76f0a2635d5a1511a22d2f0 (MD5) Previous issue date: 2017-10-20 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Este estudo mostra o funcionamento dos processos de oxidação de matéria orgânica junto com os processos simultâneos de nitrificação e desnitrificação (SND) dentro do reator aeróbio de leito fluidizado de tubos concêntricos com uma terceira câmara para gerar condições anóxicas (necessárias para garantir o processo de desnitrificação). O reator foi operado por um período de 140 dias, durante o qual a remoção de demanda química de oxigênio (DQO) e de nitrogênio total (NT), atingiu 80% e 44% respectivamente, para um tempo de detenção hidráulica (TDH) de 3 horas. A configuração dos diâmetros dos tubos concêntricos resultou em diferentes relações de áreas e, portanto em diferentes vazões para cada câmara. Na operação do reator foram aplicadas duas relações de vazões alterando o nível de esgoto dentro do reator. Os efeitos destas relações de vazões, alterou a concentração de oxigênio dissolvido (OD) entre os compartimentos aeróbio e anóxico do reator e a relação C:N, as quais foram estudadas durante o funcionamento deste sistema. Os resultados indicaram que a relação de vazões 2(4,8: 3,8: 1 para as câmaras 1, 2 e 3, respectivamente) beneficiou as remoções de DQO e NT. A concentração de OD no compartimento aeróbio apresentou valores médios de 3,57 ± 1,24 mg.L -1 , e no topo da câmara 3, a concentração de OD foi de 1,41 ± 0,78 mg.L -1 . As relações C:N que apresentaram maiores eficiências de remoção de NT foram as superiores a 10, sendo a máxima remoção de T na relação C:N de 17. O tempo de retenção celular (TRC) alcançado no reator foi de 3,16 ± 3,07 dias. Neste trabalho de pesquisa foi concluído que a configuração de três câmaras concêntricas para o reator aeróbio de leito fluidizado beneficia a remoção de NT, tornando este reator uma alternativa para o tratamento de águas residuárias domésticas. / This study shows the operation of the organic matter oxidation processes together with the simultaneous processes of nitrification and denitrification (SND) inside the aerobic fluidized bed reactor of concentric tubes with a third chamber to generate anoxic conditions (necessary to guarantee the denitrification process). The reactor operated over a period of 140 days, during which chemical oxygen demand (COD) and total nitrogen (TN) removal reached 80% and 44% respectively, for a 3 hour hydraulic detention time (HDT). The configuration of the diameters of the concentric tubes resulted in different area ratios and therefore in different flow rates for each chamber. In the reactor operation were applied two flow ratios, altering the sewage level inside the reactor. The effects of these flow rates were changing the concentration of dissolved oxygen (DO) between the aerobic and anoxic compartments of the reactor and the C: N ratio, which was studied during the operation of this system. The results indicated that the ratio of flows 2 (4.8: 3.8: 1 for chambers 1, 2 e 3, respectively) benefited the COD and TN removal. The DO concentration in the aerobic compartment presented average values of 3.57 ± 1.24 mg.L-1 , and on top of chamber 3, the DO concentration was 1.41 ± 0.78 mg.L-1 . The C:N ratios that showed higher nitrogen removal efficiencies were higher than 10, with the maximum TN removal at the C:N ratio of 17. The cell retention time reached (CRT) in the reactor was 3.16 ± 3.07 days. In this research, was concluded that three concentric chambers configuration for the aerobic fluidized bed reactor benefits the TN removal, became this reactor an alternative in domestic wastewater treatment.

Orientador: Tsunao Matsumoto / Resumo: Este estudo mostra o funcionamento dos processos de oxidação de matéria orgânica junto com os processos simultâneos de nitrificação e desnitrificação (SND) dentro do reator aeróbio de leito fluidizado de tubos concêntricos com uma terceira câmara para gerar condições anóxicas (necessárias para garantir o processo de desnitrificação). O reator foi operado por um período de 140 dias, durante o qual a remoção de demanda química de oxigênio (DQO) e de nitrogênio total (NT), atingiu 80% e 44% respectivamente, para um tempo de detenção hidráulica (TDH) de 3 horas. A configuração dos diâmetros dos tubos concêntricos resultou em diferentes relações de áreas e, portanto em diferentes vazões para cada câmara. Na operação do reator foram aplicadas duas relações de vazões alterando o nível de esgoto dentro do reator. Os efeitos destas relações de vazões, alterou a concentração de oxigênio dissolvido (OD) entre os compartimentos aeróbio e anóxico do reator e a relação C:N, as quais foram estudadas durante o funcionamento deste sistema. Os resultados indicaram que a relação de vazões 2(4,8: 3,8: 1 para as câmaras 1, 2 e 3, respectivamente) beneficiou as remoções de DQO e NT. A concentração de OD no compartimento aeróbio apresentou valores médios de 3,57 ± 1,24 mg.L -1 , e no topo da câmara 3, a concentração de OD foi de 1,41 ± 0,78 mg.L -1 . As relações C:N que apresentaram maiores eficiências de remoção de NT foram as superiores a 10, sendo a máxima remoção de T na relação C:N de 17. O te... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: This study shows the operation of the organic matter oxidation processes together with the simultaneous processes of nitrification and denitrification (SND) inside the aerobic fluidized bed reactor of concentric tubes with a third chamber to generate anoxic conditions (necessary to guarantee the denitrification process). The reactor operated over a period of 140 days, during which chemical oxygen demand (COD) and total nitrogen (TN) removal reached 80% and 44% respectively, for a 3 hour hydraulic detention time (HDT). The configuration of the diameters of the concentric tubes resulted in different area ratios and therefore in different flow rates for each chamber. In the reactor operation were applied two flow ratios, altering the sewage level inside the reactor. The effects of these flow rates were changing the concentration of dissolved oxygen (DO) between the aerobic and anoxic compartments of the reactor and the C: N ratio, which was studied during the operation of this system. The results indicated that the ratio of flows 2 (4.8: 3.8: 1 for chambers 1, 2 e 3, respectively) benefited the COD and TN removal. The DO concentration in the aerobic compartment presented average values of 3.57 ± 1.24 mg.L-1 , and on top of chamber 3, the DO concentration was 1.41 ± 0.78 mg.L-1 . The C:N ratios that showed higher nitrogen removal efficiencies were higher than 10, with the maximum TN removal at the C:N ratio of 17. The cell retention time reached (CRT) in the reactor was 3.16 ± 3.... (Complete abstract click electronic access below) / Mestre

Cette thèse propose une approche de filtrage originale, SND en abrégé pour Scoring-based Neighborhood Dominance, pour le problème d’isomorphisme de sous- graphe. En raisonnant sur des propriétés de dominance entre sommets basées sur diverses fonctions de score et de voisinage, SND apparait comme un puissant mécanisme de filtrage. Une spécialisation de SND est étudiée, elle est basée sur le nombre de chemins de longueur k comme fonction de score ainsi que trois manières de considérer le voisinage. Avec cette spécialisation, il est montré que SND est plus puissant que LAD et incomparable à SAC (Singleton Arc Consistency). L'étude expérimentale montre que SND atteint dans la plupart des cas les mêmes performances en terme de filtrage que SAC tout en étant plus rapide de plusieurs ordres de grandeurs. Cela permet de résoudre le problème d’isomorphisme de sous-graphe en étant beaucoup plus efficace que MAC et légèrement meilleur que LAD.Un solveur de contraintes est également proposé ainsi qu'une optimisation du processus de propagation de MAC. / This thesis presents anoriginal filtering approach, called SND(Scoring- based Neighborhood Dominance), for the subgraph isomorphism problem. By reasoning on vertex dominance properties based on various scoring and neigh- borhood functions, SND appears to be a filtering mechanism of strong inference potential. For example, the recently proposed method LAD is a particular case of SND. A specialization is studied of SND : by considering the number of k-length paths in graphs and three ways of relating sets of vertices. With this specialization, we prove that SND is stronger than LAD and incomparable to SAC (Single- ton Arc Consistency). Our experimental results show that SND achieves most of the time the same filtering performances as SAC (while being several orders of magnitude faster), which allows one to find subisomorphism functions far more efficiently than MAC, while slightly outperforming LAD.

Groundwater and surface water contamination has been linked in the past to inadequate or failing on-site wastewater treatment and disposal systems. The on-site wastewater systems installed in coastal areas have more potential for inflicting this kind of environmental damage. This work studied the regulatory compliance and environmental protection of the four types of on-site wastewater disposal systems found on the Mississippi Gulf Coast; i.e., vegetative rock filter, subsurface drip irrigation, sand mound, and sprinkler systems, by statistical techniques. Compliance was also evaluated for groundwater samples collected from monitoring wells installed at four corners of a disposal field. This work eventually culminated in formulation of strategy for modifying the aerobic treatment prior to disposal to help reduce nitrogen loading on the discharging environment. Process modeling and simulations were performed to optimize conditions for biological nitrogen reduction in the treatment unit by efficient management of aeration. Two separate proposals were developed, such as either running the aerator unit in a low operating dissolved oxygen concentration or intermittent aeration mode.

O processo de nitrificação e desnitrificação simultâneas (NDS) permite alcançar a remoção combinada de matérias carbonácea e nitrogenada em uma única unidade. O reator de leito estruturado, com biomassa imobilizada e recirculação interna, apresenta características positivas para que estes processos envolvidos ocorram, tais como propiciar a formação de biofilme e evitar a colmatação do leito. Esta configuração tem sido estudada com êxito em reatores em escala de bancada para tratamento de esgoto. Nesta pesquisa foi utilizado um reator de leito estruturado em escala piloto com a finalidade de avaliar sua implantação, eficiência e estabilidade tratando esgoto doméstico em condições reais para futura aplicação em pequenas comunidades, condomínios residenciais entre outros como sistema descentralizado. O reator foi construído em fibra de vidro, de formato cilíndrico, com diâmetro interno de aproximadamente 0,80 m e 2,0 m de altura. O volume total foi de aproximadamente 0,905 m3 e o volume útil de 0,642 m3. A operação foi realizada sob condições de aeração contínua e intermitente e os tempos de detenção hidráulica (TDH) testados foram de 48, 36 e 24 horas. A remoção de DQO manteve-se acima de 90% com TDH de 48 e 36 horas. A melhor eficiência de remoção de nitrogênio total foi de 72,4 ± 6,4%, sob TDH de 48 horas e a aeração intermitente, com 2 horas de aeração e 1 hora não aerada. A concentração de oxigênio dissolvido (OD) média de 2,8 ± 0,5 mg.L-1 na fase aerada e temperatura média de 24,7 ± 1,0 °C. Nesse mesmo período, a eficiência média de remoção de DQO foi de 94 ± 4 %. Apesar das dificuldades apresentadas no controle da aeração, as eficiências das remoções obtidas indicaram que o reator de leito estruturado e aeração intermitente (LEAI) se apresenta como uma alternativa promissora em escala plena, requerendo ajustes para construção e incremento da estabilidade da NDS. / The simultaneous nitrification and denitrification (SND) process allow achieving a combined nitrogen and carbon removal in a single unit. The structured-bed reactor, with immobilized biomass and internal recirculation, presents positive characteristics for occurrence of these involved processes, such as providing biofilm formation and prevent clogging. This configuration have been study successfully in a bench scale for sewage treatment. In this research was used a structured-bed reactor in a pilot scale in order to evaluate its implantation, efficiency and stability treating sewage in real conditions to future application in small communities, residential condominium among others, as decentralized treatment. The reactor was constructed with cylindrical glass fiber modules, with internal diameter of 0.8 m and 2.0 m height. The total volume was about 0.905 m3 and 0.642 m3 working volume. It was worked under continuous and intermittent aeration conditions and the tested hydraulic retention times (HRT) were 48, 36 e 24 hours. The maximum total nitrogen removal was 72.4 ± 6.4% ; the HRT was 48 hours with intermittent aeration, in which 2 hours in aeration and 1-hour nonaeration. An average dissolved oxygen (DO) concentration of 2.8 ± 0.5 mg.L-1 in the aerated time and an average temperature of 24.7 ± 1.0°C. In the same period, the average COD removal was 94 ± 4%. Despite of the difficulties presented in aeration control, the obtained removals indicated the structured-bed reactor subjected to recirculation and intermittent aeration (SBRRIA) can be a promising alternative in full scale, demanding adjustments to improve the reactor construction and the SND stability.

O processo de nitrificação e desnitrificação simultâneas (NDS) permite alcançar a remoção combinada de matérias carbonácea e nitrogenada em uma única unidade. O reator de leito estruturado, com biomassa imobilizada e recirculação interna, apresenta características positivas para que estes processos envolvidos ocorram, tais como propiciar a formação de biofilme e evitar a colmatação do leito. Esta configuração tem sido estudada com êxito em reatores em escala de bancada para tratamento de esgoto. Nesta pesquisa foi utilizado um reator de leito estruturado em escala piloto com a finalidade de avaliar sua implantação, eficiência e estabilidade tratando esgoto doméstico em condições reais para futura aplicação em pequenas comunidades, condomínios residenciais entre outros como sistema descentralizado. O reator foi construído em fibra de vidro, de formato cilíndrico, com diâmetro interno de aproximadamente 0,80 m e 2,0 m de altura. O volume total foi de aproximadamente 0,905 m3 e o volume útil de 0,642 m3. A operação foi realizada sob condições de aeração contínua e intermitente e os tempos de detenção hidráulica (TDH) testados foram de 48, 36 e 24 horas. A remoção de DQO manteve-se acima de 90% com TDH de 48 e 36 horas. A melhor eficiência de remoção de nitrogênio total foi de 72,4 ± 6,4%, sob TDH de 48 horas e a aeração intermitente, com 2 horas de aeração e 1 hora não aerada. A concentração de oxigênio dissolvido (OD) média de 2,8 ± 0,5 mg.L-1 na fase aerada e temperatura média de 24,7 ± 1,0 °C. Nesse mesmo período, a eficiência média de remoção de DQO foi de 94 ± 4 %. Apesar das dificuldades apresentadas no controle da aeração, as eficiências das remoções obtidas indicaram que o reator de leito estruturado e aeração intermitente (LEAI) se apresenta como uma alternativa promissora em escala plena, requerendo ajustes para construção e incremento da estabilidade da NDS. / The simultaneous nitrification and denitrification (SND) process allow achieving a combined nitrogen and carbon removal in a single unit. The structured-bed reactor, with immobilized biomass and internal recirculation, presents positive characteristics for occurrence of these involved processes, such as providing biofilm formation and prevent clogging. This configuration have been study successfully in a bench scale for sewage treatment. In this research was used a structured-bed reactor in a pilot scale in order to evaluate its implantation, efficiency and stability treating sewage in real conditions to future application in small communities, residential condominium among others, as decentralized treatment. The reactor was constructed with cylindrical glass fiber modules, with internal diameter of 0.8 m and 2.0 m height. The total volume was about 0.905 m3 and 0.642 m3 working volume. It was worked under continuous and intermittent aeration conditions and the tested hydraulic retention times (HRT) were 48, 36 e 24 hours. The maximum total nitrogen removal was 72.4 ± 6.4% ; the HRT was 48 hours with intermittent aeration, in which 2 hours in aeration and 1-hour nonaeration. An average dissolved oxygen (DO) concentration of 2.8 ± 0.5 mg.L-1 in the aerated time and an average temperature of 24.7 ± 1.0°C. In the same period, the average COD removal was 94 ± 4%. Despite of the difficulties presented in aeration control, the obtained removals indicated the structured-bed reactor subjected to recirculation and intermittent aeration (SBRRIA) can be a promising alternative in full scale, demanding adjustments to improve the reactor construction and the SND stability.

Esta pesquisa teve por objetivo a produção de elementos para a avaliação da viabilidade de se agregar novas tecnologias a uma estação de tratamento de esgoto sanitário pelo processo de lodo ativado com aeração prolongada em escala real. O estudo foi desenvolvido na ETE Limoeiro da SABESP, localizada no Município de Presidente Prudente/SP. Foi estudado a remoção biológica de nitrogênio via nitrificação e desnitrificação simultânea (NDS), controlando-se a idade do lodo e o sistema de fornecimento de ar por meio de sensores de concentração de oxigênio dissolvido de luminescência (LDO), amônia e nitrato, e de potencial de óxido-redução instalados nos tanques de aeração. No controle do processo foi avaliado a emissão de óxido nitroso e determinado os parâmetros cinéticos da nitrificação e desnitrificação, por meio de testes respirométricos. Foi utilizado a técnica de biologia molecular de FISH para identificação das bactérias nitritantes, nitratantes e desnitrificantes envolvidas no processo da NDS. Em termos de remoção de matéria orgânica, a qualidade do efluente final foi compatível aos processos de lodos ativados tradicionais com OD na faixa de 2,0 mgO2/L, com eficiências entre 85 a 95% de DQO e DBO. Os resultados demostraram que a nitrificação do esgoto ocorreu de forma eficiente, resultando concentrações de amônia no efluente final menores que 3,8 mgNNH3/ L. Os resultados de concentração de nitrato no efluente final do processo de lodos ativados demonstram a expressiva eficiência do processo de desnitrificação, resultando em concentrações de nitrato no efluente final menores que 2,5 mgNNO3/ L. A otimização do sistema de aeração resultou em uma economia média de 34% com o gasto de energia elétrica para aeração do sistema. Conclui-se que o processo de lodos ativados com nitrificação e desnitrificação simultânea de fluxo contínuo é uma alternativa promissora de tratamento, a ser considerada tano nos estudos de concepção para implantação de novas estações como na adaptação de processos de lodos ativados já existentes. / This research aimed at the production of elements for assessing the feasibility of adding new technologies to a sewage treatment plant for sludge process with extended aeration activated in real scale. The study was developed in ETE Limoeiro SABESP, located in the Municipality of President Prudente / SP. Biological nitrogen removal by biological nitrification and simultaneous denitrification was studied (NDS), controlling the sludge age and the system supply air through oxygen concentration sensors dissolved luminescence (LDO), ammonia and nitrates, and the redox potential installed in the aeration tanks. In process control, the emission of nitrous oxide was evaluated and determined the kinetic parameters of nitrification and denitrification, by molecular biology techniques FISH was the identification nitritantes bacteria and denitrifying nitratantes involved in the process of NDS. In terms of removal of organic matter, the effluent quality is compatible to traditional activated sludge processes, with efficiencies from 85 to 95% of COD and BOD. The results showed that the sewer nitrification took place efficiently, resulting in ammonia concentrations in the final effluent smaller than 3.8 NH3 mgN / L. The nitrate concentration results in the final effluent from the activated sludge process demonstrate the significant efficiency of denitrification process, resulting in nitrate concentration in the final effluent smaller than 2.5 mgNNO3 / l. Optimization of the aeration system resulted in an average savings of 34% with the electricity spent aeration system. We conclude that the activated sludge process with nitrification and denitrification simultaneous streaming is a promising alternative treatment to be considered thane in design studies for implementation of new stations as the adaptation of sludge processes already activated.

O fenômeno conhecido como Nitrificação e Desnitrificação Simultânea (SND) significa que em um mesmo reator ocorre simultaneamente a nitrificação e a desnitrificação, sob condições de operações idênticas, podendo ser justificada principalmente pela teoria de microambiente no floco ou biofilme. Assim, em um único reator, sob condições controladas de oxigênio dissolvido (OD) e elevados tempos de residênciacelular épossível que ocorra a nitrificação e a criação de zonas anóxicas no interior dos flocos ou biofilme para a ocorrência da desnitrificação. Neste sentido, a tecnologia MBBR/IFAStem como característicaelevado tempo de residência celular do biofilme formado nos meios suporte presentes no reator. Deste modo, neste estudo avaliou-se a remoção de nitrogênio via SND em um sistema IFAS quando submetido a diferentes concentrações de OD e Tempo de DetençãoHidraulica de 5,5 e 11 horas, tratando efluente sanitário e efluente sintético. Os resultados experimentais demonstraram que pode ser possível desenvolver efetiva SND com concentrações de OD média de 1,0 mg.L-1 e 1,5 mg.L-1. Sendo que, foram obtidas eficiência média de remoção de NTde cerca de 68% e concentrações médias efluente de N-NH4 de aproximadamente 5,0 mg L-1, de N-NO3 inferiores a 4,5 mg L-1 e de N-NO2 em torno de 0,1 mg L-1, e com eficiência média de remoção DQO solúvel acima de 90%, quando empregado efluente sintético. Ademais, por meio da avaliação da emissão de Óxido Nitroso (N2O), foi possível comprovar que a desnitrificação ocorreu de forma efetiva. / The phenomenon known as Simultaneous Nitrification and Denitrification (SND) means that in the same reactor simultaneously occurring nitrification and denitrification under identical operations conditions, and can be mainly explained by the microenvironment theory inside the floc or biofilm. Therefore, in a single reactor under controlled conditions of dissolved oxygen (DO) and high Solids Retention Time (SRT) it is possible that the nitrification happens and the creation of anoxic zones within the flocsor biofilm to the occurrence of the denitrification. In this sense, the MBBR/IFAS technology is characterized by high SRT of the biofilm formed on the carriers present in the reactor. Thereby,this work evaluated the nitrogen removal through SND in a IFAS system when submitted to different DOconcentrations and Hydraulic Retention Time of 5.5 hours and 11.0 hours, treating domestic and synthetic effluents. The experimental results showed that it may be possible to develop effective SND withaverage concentrations of DO of 1.0 mg L-1 and 1.5 mg L-1. It was obtained a TN average removal efficiency about 68%, and an average effluent concentration of NH4-N of approximately 5.0 mg L-1, of NO3-N less than 4.5 mg L-1 and ofNO2-N around 0.1 mg L-1, and an average CODsoluble removal efficiency above 90%, employing synthetic effluent. Furthermore, by evaluating the Nitrous Oxide(N2O) emission, it was possible to prove that the denitrification occurred effectively.