Call number: LD2668 .T4 1953 E9 / Master of Science

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No description available.

Thesis (M.Com. (Development Theory and Policy))--University of the Witwatersrand, Faculty of Commerce, Law and Management, School of Economic and Business Sciences. / This research paper analyses the Zambian soya bean value chain and serves to explore and investigate to what extent trade theory can be used to explain this value chain. Soya bean production in Zambia has grown vastly within the last four years, owing to this growth, Zambia has instantly become a net exporter of soya bean cake. Most of the soya bean cake produced is consumed within the country. The animal feed industry utilises a large percentage of the soya cake in order to produce animal feed for the poultry sector predominantly. The growth in the soya industry has created various linkages for example the poultry industry which is a growing industry owing to amongst other factors, the growth in the Zambian middle class. This research aims to explore the constraints, challenges and drivers of this value chain. Trade theory is used in order to explain the value chain and capture the limitations of theory in relation to the Zambian soya bean value chain. The research paper uses both a qualitative and quantitative methodology. Interviews were used to source information and quantitative data was collected from various platforms

Digitized by Kansas Correctional Industries

Digitized by Kansas Correctional Industries

Digitized by Kansas Correctional Industries

Digitized by Kansas Correctional Industries

Digitized by Kansas Correctional Industries

Wong, Cheuk Hon. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 69-75). / Abstracts in English and Chinese. / Thesis Committee --- p.ii / Statement --- p.iii / Abstract --- p.iv / 摘要 --- p.V / Acknowledgements --- p.vi / General Abbreviations --- p.viii / Abbreviations of Chemicals --- p.X / List of Figures --- p.xi / List of Tables --- p.xii / Table of Contents --- p.xiii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Food insecurity and abiotic stress --- p.1 / Chapter 1.2 --- Overview of the molecular basis of salt tolerance in plants --- p.4 / Chapter 1.2.1 --- Stress perception --- p.4 / Chapter 1.2.2 --- Signal transduction --- p.5 / Chapter 1.2.2.1 --- ABA dependent pathway --- p.6 / Chapter 1.2.2.2 --- ABA independent pathway --- p.8 / Chapter 1.2.2.3 --- The SOS pathway --- p.9 / Chapter 1.2.3 --- Abiotic stress tolerance mechanisms --- p.9 / Chapter 1.2.3.1 --- Ion transporters --- p.10 / Chapter 1.2.3.2 --- Osmolytes --- p.10 / Chapter 1.2.3.3 --- Antioxidants and ROS scavenging genes --- p.11 / Chapter 1.2.3.4 --- Late Embryogenesis Abundant proteins --- p.12 / Chapter 1.2.3.5 --- "Heat shock factor, heat shock element and heat shock protein" --- p.12 / Chapter 1.2.3.5.1 --- Hsp families --- p.14 / Chapter 1.2.3.5.2 --- Hsp network --- p.18 / Chapter 1.3 --- Pervious studies on GmDNJl --- p.19 / Chapter 1.4 --- "Hypothesis, significances and objectives of this project" --- p.19 / Chapter Chapter 2 --- Materials and Methods --- p.21 / Chapter 2.1 --- Materials --- p.21 / Chapter 2.1.1 --- "Plants, strains, and vectors" --- p.21 / Chapter 2.1.2 --- Chemicals and reagents --- p.24 / Chapter 2.1.3 --- Commercial kits --- p.25 / Chapter 2.1.4 --- Primers --- p.26 / Chapter 2.1.5 --- Equipment and facilities --- p.27 / Chapter 2.1.6 --- Sequencing --- p.27 / Chapter 2.1.7 --- "Buffer, solution and medium" --- p.27 / Chapter 2.1.8 --- Software --- p.28 / Chapter 2.2 --- Methods --- p.29 / Chapter 2.2.1 --- Growth conditions of bacterial and yeast cultures --- p.29 / Chapter 2.2.2 --- Growth conditions and multiple abiotic stress treatments of Glycine max for GmDNJl expression study --- p.29 / Chapter 2.2.2.1 --- "RNA extraction, cDNA synthesis of real-time PCR" --- p.30 / Chapter 2.2.3 --- Growth conditions and multiple abiotic stress treatments of Arabidopsis thaliana for root length essay --- p.30 / Chapter 2.2.3.1 --- Surface sterilization of Arabidopsis thaliana seeds --- p.30 / Chapter 2.2.3.2 --- Abiotic stress treatments of Arabidopsis thaliana for root length essay --- p.31 / Chapter 2.2.4 --- Multiple abiotic stress treatments of Tobacco Bright Yellow-2 (BY2) cell for cell viability assay --- p.31 / Chapter 2.2.4.1 --- Construction of transgenic GmDNJl BY2 cell lines --- p.31 / Chapter 2.2.4.2 --- DNA extraction of transgenic GmDNJl BY2 cell lines by CTAB method --- p.32 / Chapter 2.2.4.3 --- PCR screening of transgenic GmDNJl BY2 cell lines --- p.33 / Chapter 2.2.4.4 --- Gene expression checking of GmDNJl in transgenic BY2 cell lines by real-time PCR --- p.34 / Chapter 2.2.4.4.1 --- RNA extraction of BY2 cell --- p.35 / Chapter 2.2.4.4.2 --- cDNA synthesis of BY2 cell RNA --- p.35 / Chapter 2.2.4.4.3 --- Real-time PCR ofBY2 cell cDNA --- p.36 / Chapter 2.2.4.5 --- Multiple abiotic stress treatments of BY2 cell lines for cell viability assay --- p.37 / Chapter 2.2.5 --- Yeast two-hybrid experiment --- p.37 / Chapter 2.2.5.1 --- Subcloning oi GmDNJl into pGBKT7 --- p.37 / Chapter 2.2.5.2 --- Transformation of pGBKT7 into Y187 --- p.38 / Chapter 2.2.5.3 --- Toxicity test of GAL4 GmDNJl fusion protein in Y187 --- p.38 / Chapter 2.2.5.4 --- Western blot analysis to check the expression of GAL4-GmDNJl in Y187 --- p.39 / Chapter 2.2.5.5 --- Screening of GmDNJl interacting protein partners by yeast mating --- p.39 / Chapter 2.2.5.6 --- Identification of positive interaction partners by retrieving plasmid from diploid yeast --- p.40 / Chapter Chapter 3 --- Results --- p.41 / Chapter 3.1 --- "Expression of GmDNJl was induced under dehydration stress, NaHCOs stress, oxidative stress and heat stress" --- p.41 / Chapter 3.2 --- Ectopic expression of GmDNJl in transgenic Arabidopsis does not confer extra tolerance under multiple stresses in root length essay --- p.44 / Chapter 3.3 --- Construction of transgenic GmDNJl BY2 cell --- p.47 / Chapter 3.3.1 --- PCR screening of GmDNJl in transgenic BY2 cell lines --- p.47 / Chapter 3.3.2 --- Gene expression checking of GmDNJl in transgenic BY2 cell lines by real-time PCR --- p.49 / Chapter 3.3.3 --- Ectopic expression of GmDNJl in transgenic BY2 cell lines do not enhance tolerance under multiple stresses in cell viability essay --- p.51 / Chapter 3.4 --- Yeast two-hybrid experiment --- p.53 / Chapter 3.4.1 --- Subcloning of pGBKTl-GmDNJl --- p.54 / Chapter 3.4.2 --- Toxicity test of GAL4 DNA BD-c-Myc-GmDNJ 1 fusion protein inY187 --- p.54 / Chapter 3.4.3 --- Western blotting showing expression of GAL4 DNA BD -c-Myc-GmDNJl in Y187 --- p.55 / Chapter 3.4.4 --- Screening of GmDNJl putative protein interacting partners by yeast mating --- p.56 / Chapter Chapter 4 --- Discussion --- p.60 / Chapter 4.1 --- "Expression of GmDNJl was induced under dehydration stress, NaHCOs stress, oxidative stress and heat stress" --- p.60 / Chapter 4.2 --- Ectopic expression of GmDNJl in transgenic Arabidopsis and BY2 cells did not confer extra tolerance under multiple stresses in root length essay --- p.63 / Chapter 4.3 --- Yeast two-hybrid experiment --- p.64 / Chapter 4.4 --- Putative function of GmDNJl --- p.65 / Chapter 4.5 --- Future Perspectives --- p.66 / Chapter Chapter 5 --- Conclusion --- p.68 / Chapter Chapter 6 --- References --- p.69 / Chapter Appendix I --- Chemical and reagents --- p.76 / Chapter Appendix II --- "Buffer, solution, gel and medium formulation" --- p.78 / Chapter Appendix III --- Supplementary results --- p.81