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Intron retention and recognition in the microsporidian encephalitozoon cuniculiLee, Renny 11 1900 (has links)
Microsporidia are unicellular fungi that are intracellular parasites of animals, including
humans. They are both complex and simple, armed with a sophisticated infection
apparatus and possessing the smallest eukaryotic nuclear genomes. The microsporidian
Encephalitozoon cuniculi has a genome size of 2.9 Mb, which is smaller than many
bacterial genomes. Genome reduction and compaction in size, content, and form has been
interpreted as an adaptation to parasitism. One of the effects of genome size reduction
concerns intron evolution — E. cuniculi has retained only a few extremely short
spliceosomal introns.
This thesis examines the splicing of introns in the spore stage. The introns were retained
in spores, suggesting life-stage specific splicing and splicing inhibition. How the short
introns are recognized was also examined. Unique splicing signal motifs were predicted,
and were used to find additional introns. The intron density was doubled for this species,
and I also obtained data that counter current views about intron evolution in compacted
genomes with low intron densities. I also predict that E. cuniculi introns are recognized
in a unique way by the spliceosome.
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Photonic-Crystal-Fibers-Based Interferometers by Misaligned SplicingHsiao, Li-Tai 28 July 2010 (has links)
We propose a PCF-based interferometer by the misaligned splicing method. The PCF-based interferometers are composed of a photonic crystal fiber (PCF) and single-mode fibers (SMFs) which are spliced with lateral offsets. As the wave propagates at the first splicing point, the lateral offset will excite the cladding mode and the core mode simultaneously. As the two modes reach the other splicing point, they are recombined and coupled into another SMF. Thus, we can observe the interference pattern resulted from the phase difference between the two modes. In addition, as the length of PCF is increased, the average splicing of the interference fringes become smaller in the same measured range.
We demonstrate the applications of the PCF-based interferometer as temperature, bending, and the surrounding refractive index sensors. The temperature sensitivity for the 2-cm and 4-cm PCF-based-interferometer is 3.9.~4.3pm/¢XC and 3.5~4.3pm/¢XC, respectively. As we increase the surrounding refractive index, the curves move toward longer wavelengths. Besides, the measured bending sensitivity of the PCF-based interferometer is 3.8~4.2nm/m-1.
We also fabricated the liquid-filled-PCF-based interferometers by using the vacuum filling method. The measured bending sensitivity of the liquid-filled-PCF-based interferometer is 8.5nm/m-1 which is higher than that of the PCF-based interferometer. The measured surrounding refractive index sensitivity is insensitive. Thus, this liquid-filled-PCF-based interferometer can also be utilized as a sensor.
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Catalytic mechanisms of group II intron self-splicing : parallels with the spliceosome /Gordon, Peter M. January 2001 (has links)
Thesis (Ph. D.)--University of Chicago, Dept. of Biochemistry and Molecular Biology, August 2001. / Includes bibliographical references. Also available on the Internet.
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Nuclear genes that promote splicing of the chloroplast group-I 23S rRNA intron and an organelle intron database, FUGOIDLi, Fei. January 2002 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2002. / Vita. Includes bibliographical references. Available also from UMI Company.
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Proteins required for chloroplast group II intron splicing : CRS2 and its associated factors /Ostheimer, Gerard Joseph, January 2003 (has links)
Thesis (Ph. D.)--University of Oregon, 2003. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 111-124). Also available for download via the World Wide Web; free to University of Oregon users.
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Computational analysis of the U2 snRNA-intron duplexXu, Darui. Greenbaum, Nancy L. January 2003 (has links)
Thesis (M.S.)--Florida State University, 2003. / Dr. Nancy L. Greenbaum, Florida State University, College of Arts and Sciences, Dept. of Chemistry and Biochemistry. A Title and description from dissertation home page (viewed Mar. 12, 2004). Includes bibliographical references.
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Structural studies of a group I intron splicing factor and a continuous three-dimensional DNA latticePaukstelis, Paul John 28 August 2008 (has links)
Not available / text
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Nuclear genes that promote splicing of the chloroplast group-I 23S rRNA intron and an organelle intron database, FUGOIDLi, Fei 16 May 2011 (has links)
Not available / text
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19 |
Intron retention and recognition in the microsporidian encephalitozoon cuniculiLee, Renny 11 1900 (has links)
Microsporidia are unicellular fungi that are intracellular parasites of animals, including
humans. They are both complex and simple, armed with a sophisticated infection
apparatus and possessing the smallest eukaryotic nuclear genomes. The microsporidian
Encephalitozoon cuniculi has a genome size of 2.9 Mb, which is smaller than many
bacterial genomes. Genome reduction and compaction in size, content, and form has been
interpreted as an adaptation to parasitism. One of the effects of genome size reduction
concerns intron evolution — E. cuniculi has retained only a few extremely short
spliceosomal introns.
This thesis examines the splicing of introns in the spore stage. The introns were retained
in spores, suggesting life-stage specific splicing and splicing inhibition. How the short
introns are recognized was also examined. Unique splicing signal motifs were predicted,
and were used to find additional introns. The intron density was doubled for this species,
and I also obtained data that counter current views about intron evolution in compacted
genomes with low intron densities. I also predict that E. cuniculi introns are recognized
in a unique way by the spliceosome.
|
20 |
Genetic studies of self-splicing RNAs in bacteriophage T4Deyoung, Katherine Leigh 12 1900 (has links)
No description available.
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