Call number: LD2668 .T4 1978 K85 / Master of Science

The lipid composition of swine is characteristic of the species and is also a reflection of their dietary history. Other investigators have established that the physical properties and composition of swine depot fat may be altered by variation of the dietary lipid. Dietary regimens employing vegetable oil-bearing materials and their effect on meat quality has received the attention of animal nutritionists for a considerable period. Less is known about the nutritive value of the more highly unsaturated lipids, such as fish oils, and their effect on the lipid composition of swine tissues. Even less is known about the fate of oxidized fish oil lipids in swine rations. This research had for its purpose the investigation of the fate in swine of the dietary marine lipid, menhaden oil, and its effect on composition of depot lipids. The state of autoxidation of the oil and the effect of certain antioxidants on the fatty acid composition of swine was determined. Growth studies on swine were made using menhaden oil of various oxidation states as the dietary lipid. The effect of antioxidants, ∝-tocopherol acetate and ethoxyquin, was also investigated. At the conclusion of the diet trials, tissue samples, representing the five dietary regimens, were removed for analysis. The fatty tissues examined were outer back fat, inner back fat, and kidney fat. Samples of liver tissue for lipid analysis were also taken. The lipids from the respective tissues were extracted and interesterified with methanol to yield the methyl esters of the fatty acids. Hydrogenation of the unsaturated methyl esters for chain length confirmation was carried out. Qualitative and quantitative gas-liquid chromatographic analysis of the unhydrogenated and hydrogenated methyl esters of the fatty acids were performed on diethylene glycol succinate column. The results of these investigations showed that a particular dietary fatty acid can be selectively deposited in animal tissues. Long chain polyunsaturated fatty acids of menhaden oil, such as 20:5, 22:5, 22:6 were sparsely deposited in adipose tissues. The depot fat composition showed a mixture of characteristic menhaden oil fatty acids with the typical fat synthesized by swine. Fatty acid composition of tissue from swine fed oxidized menhaden oil with and without antioxidants showed very similar fatty acid composition as those fed fresh menhaden oil. The results of this investigation supported the beneficial effect of vitamin E (∝-tocopherol acetate) and ethoxyquin as in vivo antioxidants. / Graduation date: 1964

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A dissertation submitted to the Faculty of Medicine, University of the Witwatersrand, Johannesburg in fulfilment of the Degree of Master of Science in the branch of Medicine / The guinea pig clears circulating tissue ferritin in a manner different from other mammals. Previous work in man and in rats has shown that injected tissue ferritin is removed from the plasma predominantly by the liver; however, in the guinea pig it is cleared predominantly by red cell precursors and furthermore, the ferritin iron is utilised directly for haem synthesis. During pregnancy, the foeto-placental complex also has a high requirement for iron. / IT2018

Digitized by Kansas Correctional Industries

Digitized by Kansas Correctional Industries

Typescript. / Digitized by Kansas Correctional Industries

Digitized by Kansas Correctional Industries

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Thesis (M.S.)--Ohio State University. 1984. / Includes bibliographical references (leaves 90-95). Available online via OhioLINK's ETD Center