Thesis (M.D.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 228-261).

Thesis (M.D.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 228-261) Also available in print.

by Poon Lit Man. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 97-113). / Title page --- p.i / Acknowledgements --- p.ii / Table of contents --- p.iii / List of tables --- p.vii / List of figures --- p.viii / Abbreviations --- p.x / Abstract --- p.xi / Chapter Chapter 1: --- General Introduction --- p.1 / Chapter 1.1 --- Sacral agenesis --- p.2 / Chapter 1.1.1 --- Skeletal anomalies --- p.3 / Chapter 1.1.2 --- Neurological anomalies --- p.4 / Chapter 1.1.3 --- Other anomalies --- p.5 / Chapter 1.1.4 --- Etiology --- p.5 / Chapter 1.1.5 --- Pathogenetic mechanism of SA --- p.7 / Chapter 1.2 --- Retinoids --- p.8 / Chapter 1.2.1 --- RA in embryonic development --- p.9 / Chapter 1.2.2 --- Teratogenic effect of RA in embryonic development --- p.10 / Chapter 1.3 --- Strategy of the thesis --- p.13 / Chapter Chapter 2: --- General Materials and Methods --- p.15 / Chapter 2.1 --- Mouse maintenance and mating method --- p.16 / Chapter 2.2 --- All-trans RA preparation and injection --- p.16 / Chapter 2.3 --- Dissection of embryos --- p.16 / Chapter 2.4 --- Preparation of histological sections --- p.17 / Chapter 2.4.1 --- Dehydration and embedding --- p.17 / Chapter 2.4.2 --- Sectioning --- p.18 / Chapter 2.4.3 --- Haematoxylin and eosin staining --- p.18 / Chapter 2.5 --- Plasmid preparation --- p.19 / Chapter 2.5.1 --- Competent cells preparation --- p.19 / Chapter 2.5.2 --- Bacterial transformation --- p.19 / Chapter 2.5.3 --- Mini-scale preparation of plasmid DNA --- p.20 / Chapter 2.6 --- In situ hybridization --- p.21 / Chapter 2.6.1 --- Sample preparation --- p.21 / Chapter 2.6.2 --- Probe synthesis --- p.21 / Chapter 2.6.3 --- Hybridization --- p.23 / Chapter 2.6.4 --- Post-hybridization wash and antibody labeling --- p.24 / Chapter 2.6.5 --- Post-antibody wash and colour development --- p.25 / Chapter 2.6.6 --- Embryo powder preparation --- p.25 / Chapter Chapter 3: --- Time and Dose Responses to RA --- p.26 / Chapter 3.1 --- Introduction --- p.27 / Chapter 3.1.1 --- Time response --- p.27 / Chapter 3.1.2 --- Dose response --- p.29 / Chapter 3.2 --- Materials and methods --- p.31 / Chapter 3.2.1 --- Dose response --- p.31 / Chapter 3.2.2 --- Time response --- p.31 / Chapter 3.2.3 --- Skeletal preparations and staining --- p.31 / Chapter 3.2.4 --- Early teratogenic responses to RA --- p.32 / Chapter 3.3 --- Results --- p.33 / Chapter 3.3.1 --- Dose response --- p.33 / Chapter 3.3.1.1 --- Tail length --- p.33 / Chapter 3.3.1.2 --- Vertebral pattern --- p.33 / Chapter 3.3.2 --- Time response --- p.36 / Chapter 3.3.2.1 --- Tail length --- p.36 / Chapter 3.3.2.2 --- Vertebral pattern --- p.36 / Chapter 3.3.2.3 --- Other anomalies associated with human SA --- p.40 / Chapter 3.3.3 --- Early teratogenic responses in RA-treated embryos --- p.41 / Chapter 3.4 --- Discussion --- p.44 / Chapter Chapter 4: --- RA-induced Cell Death in Tail Bud --- p.51 / Chapter 4.1. --- Introduction --- p.52 / Chapter 4.1.1 --- Cell death --- p.52 / Chapter 4.1.2 --- Methods in cell death identification --- p.54 / Chapter 4.2 --- Materials and methods --- p.57 / Chapter 4.2.1 --- Histology of tail bud region --- p.57 / Chapter 4.2.2 --- Detection of internucleosomal DNA fragmentation --- p.57 / Chapter 4.2.2.1 --- Sample collection --- p.57 / Chapter 4.2.2.2 --- DNA extraction --- p.58 / Chapter 4.2.2.3 --- Analysis of DNA fragmentation pattern by ethidium bromide staining --- p.59 / Chapter 4.2.2.4 --- Analysis of DNA fragmentation pattern by autoradiography --- p.59 / Chapter 4.2.3 --- TUNEL --- p.61 / Chapter 4.2.3.1 --- Sample collection / Chapter 57 4.2.3.2 --- In situ end-labeling --- p.62 / Chapter 4.2.4 --- In situ hybridization of Wnt-5A --- p.63 / Chapter 4.2.4.1 --- Sample collection --- p.63 / Chapter 4.2.4.2 --- Probe preparation --- p.64 / Chapter 4.3 --- Results --- p.65 / Chapter 4.3.1 --- Histological examination of cell death in tail bud --- p.65 / Chapter 4.3.2 --- Analysis of DNA fragmentation of tail bud cells --- p.66 / Chapter 4.3.3 --- TUNEL --- p.67 / Chapter 4.3.4 --- Wnt-5A gene expression --- p.68 / Chapter 4.4 --- Discussion --- p.71 / Chapter Chapter 5: --- Retinoic Acid Receptors (RARs) --- p.75 / Chapter 5.1 --- Introduction --- p.76 / Chapter 5.1.1 --- RARs and their isoforms --- p.76 / Chapter 5.1.2 --- Expression pattern of RARs during embryogenesis --- p.77 / Chapter 5.1.3 --- RAR mutants and functional redundancy of RARs --- p.78 / Chapter 5.1.4 --- RARs and SA --- p.79 / Chapter 5.2 --- Materials and methods --- p.80 / Chapter 5.2.1 --- In situ hybridization of RAR-α --- p.80 / Chapter 5.2.1.1 --- Sample collection --- p.80 / Chapter 5.2.1.2 --- Probe preparation --- p.80 / Chapter 5.2.2 --- In situ hybridization of RAR-β --- p.81 / Chapter 5.2.2.1 --- Sample collection --- p.81 / Chapter 5.2.2.2 --- Probe preparation --- p.81 / Chapter 5.2.3 --- In situ hybridization of RAR-γ --- p.82 / Chapter 5.2.3.1 --- Sample collection --- p.82 / Chapter 5.2.3.2 --- Probe preparation --- p.82 / Chapter 5.3 --- Results --- p.83 / Chapter 5.3.1 --- RAR-α gene expression --- p.83 / Chapter 5.3.2 --- RAR-β gene expression --- p.84 / Chapter 5.3.3 --- RAR-γ gene expression --- p.86 / Chapter 5.4 --- Discussion --- p.88 / Chapter Chapter 6: --- Conclusion and Future Perspectives --- p.92 / Chapter 6.1 --- Conclusion and future perspectives --- p.93 / References --- p.97 / Appendices --- p.114 / Figures --- p.118

This thesis deals with the project of Roman Catholic church including affiliated operational functions in Brno, Kohoutovice and its connection to complex urbanistic context bridging rural character buildings with housing estate. The thesis is divided into three main chapters. First chapter is the analytical part of a system, where individual elements are evaluated as well as the system as a whole. This part is followed up by systemic conception which addresses structure from the perspective of broader relations and sets its long-term goals. Last part of the thesis is the design of the church itself as a local refinement of the area with focus on its urbanistic relations, architectural and layout resolution and a work with detail.

published_or_final_version / Medicine / Master / Doctor of Medicine

No description available.

All living hominoids are characterized by taillessness and adaptations to orthograde (upright) trunk posture. Accordingly, these features have importance for our understanding of ape origins, evolutionary relationships and positional behaviors. Despite extensive study of the hominoid postcranial skeleton, researchers continue to face difficulty identifying taillessness and orthogrady from fossil material. In part, difficulties persist because although the Miocene fossil record indicates that the evolution of tail loss and orthogrady was decoupled, previous research has focused primarily on how the skeletal anatomy of extinct apes resembles that of living apes, in which these traits appear in conjunction. The remarkable diversity in tail lengths and positional behaviors exhibited by other mammals presents a valuable opportunity to employ the strength of the comparative method for testing functional hypotheses. The goal of this dissertation is to identify anatomical correlates of tail length and positional behaviors from sacral and caudal vertebral morphology among primates and other mammals in three studies. The first study examines the relationship between trabecular structure in the first sacral vertebra and positional behaviors (N= 78 primates). The second study quantifies aspects of internal (N=78 primates) and external (N= 472 mammals) sacral anatomy for correlates of relative tail length. The third study evaluates the functional morphology of caudal vertebrae among nonprehensile-tailed primates and other mammals that vary in relative tail length, offering additional insight into the anatomy associated with tail loss (N=333). The relationship between trabecular structure in the proximal sacrum and positional behaviors among living primates is somewhat unclear. Some trabecular parameters in the distal sacrum appear to have a relationship with tail length. Results support the functional links between previously and newly defined metrics from the external morphology of sacra and caudal vertebrae, and relative tail length, among primates and other mammals. Identified anatomical correlates from the extant primate sample are used to reconstruct the tail lengths of extinct primates. From the sacral data, Proconsul is reconstructed as tailless, Archaeolemur likely possessed a long tail, and Palaeopropithecus, Megaladapis and Epipliopithecus had short tails. From the caudal vertebrae data, Archaeolemur is reconstructed as possessing a long tail and Palaeopropithecus is reconstructed as having a short tail. / text

Thesis (Ph. D.)--University of Missouri--Columbia, 2005. / "May, 2005." Typescript. Vita. Includes bibliographical references (leaves 217-243). Also issued on the Internet.

京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第18106号 / 理博第3984号 / 新制||理||1574(附属図書館) / 30964 / 京都大学大学院理学研究科生物科学専攻 / (主査)教授 中務 真人, 教授 山極 壽一, 教授 疋田 努 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

The period of tumultuous religious reformation during which George Herbert lived demanded of people a strict adherence to the paradigmatic structures that prescribed the ways in which public displays of religious conviction were to be manifested. The freedom, indeed the necessity, to doubt is taken for granted by the modern reader, but for Herbert it was a matter of spiritual life and death. As country parson, he diligently labored to guide his parishioners, administer the sacraments, and exemplify the “right path.” This persona—reinforced by necessarily performative, faith-demonstrating actions—is continually destabilized by the experience of doubt, which leads Herbert to address his own persistent despair at the absence of God through poetry. His masterful use of the structural and thematic patterns of the Psalms in many of the poems of The Temple draws on the rich tradition of lament in contrast to the prescriptive, ideological agendas of the Book of Common Prayer and the Common Lectionary which privilege faith. The poems demonstrate an extensive knowledge of the epistemological foundations and history of both official Church doctrine and of medieval mystical thought and become a tool for exploring the paradoxes of human existence. His philosophical and rhetorical engagement with the Christological and ecclesiastical theology specific to Dionysian mysticism demonstrates the intensity of Herbert’s preoccupation with Divine absence and his near obsessive search for the ideal apophatic presence, that silent, knowing-unknowing that defines oneness with God. Nowhere are Herbert’s existential dilemmas more evident than in Memoriae Matris Sacrum, a sequence of poems written immediately following the death of his beloved mother, which reveals an inner life of the poet that his more controlled poetic voice of The Temple often conceals. These elegiac poems, written in Latin and Greek, show the poet as a “mourning child” and lay bare his most intimate fears about the constancy of his own faith and the uncertain terms of Christian death and resurrection embodied in the sacred ritual of the Eucharist. The poetic closure often ascribed to Herbert’s poems in fact disguises the nature of spiritual and psychological dilemmas which remain for Herbert persistent and unresolved.