Two-component systems (TCSs) are highly conserved across bacteria and are used to rapidly sense and respond to changing environmental conditions. The human pathogen Staphylococcus aureus uses the S. aureus exoprotein expression (sae) TCS to sense host signals and activate transcription of virulence factors essential to pathogenesis. Despite its importance, the mechanism by which the sae sensor kinase SaeS recognizes specific host stimuli is unknown. This thesis describes topology and mutagenesis studies of the sensing domain of SaeS, including basal expression and inducer-dependent phenotypes. Meanwhile, investigation of the sae auxiliary protein SaeP has identified a novel DNA binding function for this surface expressed lipoprotein that may be involved in fine-tuning the activity of the sae system. Overall, these structure-function studies provide insight into the sae signal transduction mechanism and raise some new questions regarding the role the sae system plays in the larger regulatory network S. aureus uses to control expression of its secreted virulence factors.

The local corrosion behavior of duplex stainless steel (DSS)is affected by a wide variety of factors. Localized corrosionof DSS frequently starts at micrometer scale inclusions orprecipitates, which are often segregated in theaustenite-ferrite boundary regions. Moreover, due to thepartitioning of the key alloying elements of ferrite (Cr andMo) and austenite (N and Ni), the local interactions betweenthe phases must also be considered. The aim of this doctoral study was to increase the knowledgeabout the local dissolution behavior of DSS in acidic-chlorideenvironments. The recent developments of new local probingtechniques have opened a new frontier in corrosion science,providing valuable local information not accessible in thepast. The local techniques used include electrochemicalscanning tunneling microscopy (EC-STM), scanning probe forcemicroscopy (SKPFM), magnetic force microscopy (MFM), andscanning Auger electron Spectroscopy (SAES), all withmicrometer or sub-micrometer resolution. With EC-STM, it was possible to monitor local dissolutionprocesses on DSS in situ, and in real time. MFM was capable ofimaging the phase distribution in DSS without the need of thetraditional surface etching, while SKPFM revealed that theVolta potential difference between the two phases wasmeasurable and significant. SAES showed that the compositiongradient at the phase boundaries is narrower than 2µm. Different types of DSSs have been studied, from low-alloyedDSS to superduplex. Higher contents of Cr, Mo and Nstrengthened both phases as well as the phase boundaries,resulting in phases having similar corrosion resistance thatshowed a more uniform dissolution behavior. However, the Voltapotential difference between the phases proved to be of thesame order for all the DSSs studied. Austenite was in generalassociated to regions displaying a more noble Volta potentialthan ferrite, resulting in a higher dissolution rate of theferrite next to the austenite phase. <b>Key words:</b>In situ, local dissolution, electrochemical,STM, SKPFM, MFM, SAES, duplex stainless steel, acidic-chloridesolution.

<p>The local corrosion behavior of duplex stainless steel (DSS)is affected by a wide variety of factors. Localized corrosionof DSS frequently starts at micrometer scale inclusions orprecipitates, which are often segregated in theaustenite-ferrite boundary regions. Moreover, due to thepartitioning of the key alloying elements of ferrite (Cr andMo) and austenite (N and Ni), the local interactions betweenthe phases must also be considered.</p><p>The aim of this doctoral study was to increase the knowledgeabout the local dissolution behavior of DSS in acidic-chlorideenvironments. The recent developments of new local probingtechniques have opened a new frontier in corrosion science,providing valuable local information not accessible in thepast. The local techniques used include electrochemicalscanning tunneling microscopy (EC-STM), scanning probe forcemicroscopy (SKPFM), magnetic force microscopy (MFM), andscanning Auger electron Spectroscopy (SAES), all withmicrometer or sub-micrometer resolution.</p><p>With EC-STM, it was possible to monitor local dissolutionprocesses on DSS in situ, and in real time. MFM was capable ofimaging the phase distribution in DSS without the need of thetraditional surface etching, while SKPFM revealed that theVolta potential difference between the two phases wasmeasurable and significant. SAES showed that the compositiongradient at the phase boundaries is narrower than 2µm.</p><p>Different types of DSSs have been studied, from low-alloyedDSS to superduplex. Higher contents of Cr, Mo and Nstrengthened both phases as well as the phase boundaries,resulting in phases having similar corrosion resistance thatshowed a more uniform dissolution behavior. However, the Voltapotential difference between the phases proved to be of thesame order for all the DSSs studied. Austenite was in generalassociated to regions displaying a more noble Volta potentialthan ferrite, resulting in a higher dissolution rate of theferrite next to the austenite phase.</p><p><b>Key words:</b>In situ, local dissolution, electrochemical,STM, SKPFM, MFM, SAES, duplex stainless steel, acidic-chloridesolution.</p>