Links between nitrification rates and betaproteobacterial ammonia oxidising bacteria (AOB) community structure in estuarine sediments were determined in relation to changes in salinity and substrate concentrations associated with these environments.  Sediment was collected from the upper, middle and lower reaches of the estuary and incubated with water amended with either a range of salinities from marine to freshwater, or a range of ammonia concentrations.  Ammonia consumption, nitrate and nitrite production were measured at regular intervals as an indicator of nitrification rates and 16S rRNA gene-targeted analysis of betaproteobacterial AOB community structure was carried out by denaturing gradient gel electrophoresis of amplified genes from original sediment, at the beginning of nitrate production, and after a period of incubation.  Salinity and ammonia concentrations were shown to influence both nitrification rates and betaproteobacterial AOB community structure in estuarine sediments, in particular increased ammonia concentrations lead to increased nitrification regardless of the origin of the sediment.  A shift in the dominant betaproteobacterial AOB community structure was observed in microcosms with both salinity and ammonia treatments, but particular treatments did not lead to the selection of a common community structure.  Members of the <i>Nitrosomonas </i>cluster 5 were selected for in most sediments and treatments, regardless of salinity and ammonia treatments, while others, such as members of the <i>Nitrosospira</i>-lineage cluster 1 group, were restricted to low ammonia microcosms.  This study indicates that community members are capable of functioning at a wide range of estuarine salinity and ammonia conditions but that these are eventually replaced by community members better suited to these conditions.

Dissertation (Ph.D.)--Auburn University, 2006. / Abstract. Vita. Includes bibliographic references.

Thesis (M. Sc.)--University of Canterbury, 2008. / Typescript (photocopy). "October 2008." Includes bibliographical references (leaves 132-138). Also available via the World Wide Web.

Thesis (Ph.D.)--Ohio University, November, 2008. / Release of full electronic text on OhioLINK has been delayed until November 30, 2010. Includes bibliographical references (leaves 265-284)

Thesis (Ph.D.)--Ohio University, November, 2008. / Title from PDF t.p. Release of full electronic text on OhioLINK has been delayed until November 30, 2010. Includes bibliographical references (leaves 265-284)

Thesis (M.S.)--Brigham Young University. Dept. of Microbiology and Molecular Biology, 2007. / Includes bibliographical references (p. 33-37).

Thesis (M.S.)--Montana State University--Bozeman, 2005. / Typescript. Chairperson, Graduate Committee: James W. Bauder. Includes bibliographical references (leaves 75-84).

Salinisation has been identified as a major cause of the progressive deterioration in the public water system in South Africa. To deal with this problem Waste Stabilisation Ponding systems have been used by the Leather Processing Industry as zero-dischaJ;ge wastewater evaporation disposal processes in water-limited inland regions of the country. While effective in the evaporation disposal function these systems are plagued by the generation of serious odour nuisance creating intractable environmental problems relating to adjacent residential communities. High loading to ponds of organic compounds, sulphides and ammonia results in strongly reducing anaerobic conditions prevailing in early parts of pond cascades. These are characterised by bright red colours due to the predominance of purple photosynthetic bacteria. Sporadic micro algal blooms of Spirulina sp. and Dunaliella sp. had been previously noted to occur on the latter ponds in these cascades, and were associated with their conversion to facultative function, with aerobic surface layers, and a marked reduction in odour release. This research programme undertook an investigation of the microbial ecology of a tannery waste stabilisation ponding system to describe factors which give rise to these blooms, and to determine whether microalgal growth may be manipulated to achieve a reliable oxygengenerating capping of the anaerobic ponds. The predominance of near pure cultures of Spin/lina platensis was demonstrated for the blooms and factors restricting its growth in the system were described. These include the interaction of ammonia and sulphide toxic effects and laboratory studies were undertaken to show how effluent loading may be regulated to enable effective growth of the cyanobacterium. At appropriate dilutions of tannery effluent an enhancement of growth was noted, compared to growth in defined mineral medium. An investigation of this phenomenon provided preliminary evidence for organic uptake by the pond micro algae and a possible contribution to heterotrophic nutrition. The manipulation of Spirulina sp. growth in a High Rate Algal Pond raceway was undertaken in outdoor pilot plant studies and the effect of microalgal capping of the anaerobic ponds in the cascade was demonstrated by activating a recycle loop from a blooming facultative pond. Heavy metal contaminants were effectively eliminated by an optimisation of the primary anaerobic pond function and precipitation as metal sulphides. Biomass was harvested and dried, during which a range of methods were evaluated. Toxicological studies were undertaken on the dried biomass using Artemia and chick assays, and feed studies showed its useful application in rations for the abalone Haliotlls midae and rainbow trout Onchorhynchlls mykiss. Based on positive independent assessment of research outcomes, a decision was made by the tanning company operating the Waste Stabilisation Ponding system, to proceed to the construction of a full-scale 2 500 m2 High Rate Algal Pond raceway. This would be used for controlled Spirlilina biomass production to effect a practical capping of the anaerobic ponds in the system, and to evaluate its commercial potential in the feed market. The Advanced Integrated Wastewater Ponding System described by Oswald (1991) provided the conceptual basis for the Algal Biotechnology process development undertaken. The studies of the microbial ecology and the biotechnological potential of this system have shown that a Spirulina-based High Rate Algal Ponding process can be engineered in such a way that saline tannery effluents may be treated to effect a significant reduction in overall pollution load, that biomass may be recovered as a value added product of the treatment process and that the operational performance of Waste Stabilisation Ponding systems, and hence their immediate environment, may be improved by the use of the High Rate Algal Pond as a retrofitted upgrading unit operation.

Thesis (M. Sc.)--University of Hong Kong, 1993.

Thesis (Ph.D.)--Murdoch University, 2008. / Thesis submitted to the Division of Science and Engineering. Includes bibliographical references (leaves 123-154).