by Koo Ching Irene. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 105-118). / Abstract also in Chinese. / Acknowledgments --- p.i / Abstract --- p.ii / Content --- p.iv / List of tables --- p.viii / List of figures --- p.x / Chapter Chapter 1: --- Introduction --- p.1 / Chapter A. --- Classification of salmonellae --- p.1 / Chapter B. --- Salmonellae enterica ser Enteritidis --- p.4 / Chapter C. --- Global increase in the prevalence of S. Enteritidis --- p.6 / Chapter D. --- Susceptibility of S. Enteritidis to antimicrobial agents --- p.13 / Chapter E. --- Methods for the epidemiological typing of S. Enteritidis --- p.17 / Chapter 1. --- Phenotypic methods --- p.17 / Chapter (1) --- Biotyping --- p.17 / Chapter (2) --- Antibiotic resistance pattern --- p.18 / Chapter (3) --- Phage typing --- p.18 / Chapter (4) --- Characterization of plasmids --- p.19 / Chapter a. --- Resistance plasmids --- p.20 / Chapter b. --- Transferability of plasmids --- p.20 / Chapter c. --- Incompatibility --- p.21 / Chapter 2. --- Molecular methods --- p.21 / Chapter (1) --- Plasmid analysis --- p.21 / Chapter a. --- Plasmid profile --- p.22 / Chapter b. --- Plasmid fingerprinting --- p.22 / Chapter (2) --- Chromosomal DNA fingerprinting --- p.24 / Chapter a. --- Restriction fragment length polymorphism (RFLP) of chromosomal DNA --- p.25 / Chapter b. --- Pulsed-field gel electrophoresis (PFGE) --- p.25 / Chapter c. --- Hybridization with specific gene probes --- p.26 / Chapter d. --- Ribotyping --- p.27 / Chapter e. --- Insertion sequence IS200 fingerprinting --- p.29 / Chapter (3) --- Polymerase chain reaction (PCR) --- p.30 / Chapter 3. --- Other --- p.32 / Chapter (1) --- Lipopoly saccharide (LPS) analysis --- p.32 / Chapter (2) --- "Whole cell protein profile analysis, fatty acid profile analysis, multilocus enzyme electrophoresis and Fourier-transform injfrared spectroscopy" --- p.33 / Chapter F. --- Epidemiology of S. Enteritidis in different parts of the world --- p.34 / Chapter G. --- Objectives --- p.35 / Chapter Chapter 2: --- Materials and Methods --- p.36 / Materials --- p.36 / Methods --- p.38 / Chapter A. --- Identification --- p.38 / Chapter 1. --- Biochemical tests --- p.38 / Chapter 2. --- Serotyping --- p.38 / Chapter B. --- Antimicrobial susceptibility testing --- p.39 / Chapter C. --- Characterization of β-lactamases --- p.39 / Chapter 1. --- Extraction of β-lactamases --- p.39 / Chapter 2. --- Determination of isoelectric points (pIs) --- p.41 / Chapter D. --- Characterization ofplasmids --- p.42 / Chapter 1. --- Genetic studies --- p.42 / Chapter (1) --- Transferability of resistance plasmids --- p.42 / Chapter (2) --- Mobilization of resistances --- p.43 / Chapter 2. --- Molecular studies --- p.44 / Chapter (1) --- Plasmid profile analysis --- p.44 / Chapter a. --- Plasmid extraction --- p.44 / Chapter b. --- Agarose gel electrophoresis --- p.45 / Chapter (2) --- Plasmid DNA fingerprinting --- p.45 / Chapter a. --- Preparation of pure plasmid DNA --- p.46 / Chapter b. --- Preparation of individual plasmid from strains harbouring more than one plasmid --- p.46 / Chapter c. --- Restriction endonuclease digestion of plasmid DNA --- p.47 / Chapter E. --- Total DNA fingerprinting --- p.47 / Chapter 1. --- Total DNA preparation --- p.48 / Chapter 2. --- Restriction endonuclease digestion of total DNA --- p.48 / Chapter 3. --- Ribotyping --- p.49 / Chapter (1) --- Restriction enzyme digestion of total DNA --- p.49 / Chapter (2) --- Transfer of DNA fragments to solid support --- p.49 / Chapter (3) --- Reverse transcription of rRNA into cDNA and labelling of cDNA --- p.50 / Chapter (4) --- Hybridization --- p.50 / Chapter (5) --- Detection of hybridized fragments --- p.51 / Chapter 4. --- AP-PCR (Arbitrary primed-PCR) --- p.51 / Chapter F. --- Experimental design --- p.52 / Chapter Chapter 3: --- Results --- p.54 / Chapter A. --- Prevalence of S. Enteritidis in the Prince of Wales Hospital --- p.54 / Chapter B. --- Antimicrobial susceptibilities --- p.58 / Chapter C. --- β-lactamases produced by β-lactam-resistant S. Enteritidis --- p.66 / Chapter D. --- Plasmid profile analysis --- p.66 / Chapter E. --- Characterization of resistance plasmids --- p.69 / Chapter F. --- Plasmid fingerprinting --- p.72 / Chapter G. --- Total DNA fingerprinting --- p.76 / Chapter H. --- Ribotyping --- p.82 / Chapter I. --- AP-PCR --- p.85 / Chapter J. --- "Correlation of plasmid analysis, total DNA fingerprinting, ribotyping and AP-PCR results" --- p.88 / Chapter Chapter 4: --- Discussion --- p.91 / Chapter A. --- Prevalence of S. Enteritidis --- p.91 / Chapter B. --- Susceptibilities of S. Enteritidis to antimicrobial agents --- p.92 / Chapter C. --- Evaluation of methods for epidemiological typing of S. Enteritidis --- p.94 / Chapter D. --- Molecular epidemiology of S. Enteritidis in Hong Kong --- p.99 / Chapter E. --- Areas for future research --- p.102 / References --- p.105 / Appendix --- p.119