No description available.

我們在大豆的耐鹽候選基因中進行了篩選和調查，確定了一個穀胱甘肽-S -轉移酶（Glutathion-S-transferase ）基因（ GmGSTL1 ）具抗鹽特性，其表達量也跟隨鹽處理上調。系統發育分析表明，GmGSTL1 屬於LAMBDA 類，文獻對這類蛋白功能的記載甚少。我們在異源系統，包括煙草BY- 2 細胞和擬南芥，測試其保護細胞/植物對鹽脅迫的功能。結果表示GmGSTL1 轉基因細胞的活性氧積累比對照顯著降低，存活率也有所改善。同樣，轉基因擬南芥在鹽處理壓力下的症狀也得以緩解。為了進一步剖析GmGSTL1 的保護機制，我們在大豆葉片中提取多元酚，並發現兩個候選黃酮（槲皮素，山奈酚）與GmGSTL1 起相互作用。槲皮素的外源性應用同樣可以緩解細胞/植物在鹽脅迫下的症狀，表示槲皮素在功能上與GmGSTL1 相約。 / In a survey of candidate genes located in the salinity tolerance locus of soybean, we identified a putative glutathione-S-transferase (GST) gene (GmGSTL1) which was up-regulated in response to salt treatment. Phylogenetic analyses revealed that this putative GST belongs to the Lambda class, a plant-specific group with unknown functions. We expressed GmGSTL1 in heterologous systems, including tobacco BY-2 cells and Arabidopsis thaliana, to test its ability to protect cell/plant against salinity stress. Compare to the wild type control, we observed a marked reduction of ROS accumulation in transgenic cells under salt treatment, and their survival rate was also improved. Similarly, expression of GmGST1 in transgenic A. thaliana also alleviated stress symptoms under salt treatment. To further address the possible protective mechanisms of GmGSTL1, we identified two candidate flavonoid interactants (quercetin and kaemferol) of the GmGSTL1 protein from soybean leaf extract. Exogenous application of quercetin could reduce salinity-induced ROS accumulation in BY-2 cells and leaf chlorosis in A. thaliana. / Detailed summary in vernacular field only. / Chan, Ching. / Thesis (Ph.D.) Chinese University of Hong Kong, 2014. / Includes bibliographical references (leaves 80-104). / Abstracts also in Chinese.

Salinity problems seriously affect agricultural production by reducing crop yield and arable land. The evaluation of potato genotypes (Solanum spp.) for their salinity (NaCl) tolerance in conventional field trials is time consuming and labour intensive. The results are often confounded by many field and environmental variations. In vitro bioassays can overcome some of these difficulties by providing faster, more convenient and dependable methods for screening and selection of salt tolerant potato genotypes. The objective of this research was to develop in vitro bioassay methods for screening and selection of salt tolerant potato. Under in vitro NaCl stress conditions, seed germination, early seedling growth, and single-node cutting bioassays were used to evaluate salinity tolerance. The selected genotypes were further tested with three in vitro bioassays (single-node cuttings, root tip segments, and microtuberization). The rankings of potato cultivar salinity tolerance were similar in these bioassays. The single-node cutting bioassay was recommended because it was simpler to perform than the root tip segment and microtuberization bioassays and did not exclude certain genotypes as did the microtuberization bioassay. The in vitro bioassay rankings were compared with yield ranking in field lysimeters. In both the in vitro and in vivo saline stress experiments, cvs. Kennebec and Russet Burbank were more salt tolerant than Norland. The tubers and microtubers harvested from previous experiments were tested in the greenhouse to investigate salinity carry-over effect for seed tuber production. There was no apparent residual carry-over effect found. Microtuber yield increase in the presence of low NaCl concentration was induced primarily by specific ion (Na+), and not osmotic effects. This research clearly indicated that in vitro bioassays are relatively simple, rapid, convenient, repeatable, and agree with the field lysimeter results. They can be used to substitute for f

This research was carried out to identify salt tolerant potato genotypes in vitro among 131 tetraploid potato cultivars (Solanum tuberosum), 9 diploid simple hybrid clones (4 clones of S. chacoense $ times$ S. tuberosum, 4 clones of S. phureja/S. stenotomum $ times$ S. tuberosum, and 1 clone of S. tuberosum $ times$ S. tuberosum), 1 primitive cultivated diploid S. phureja/S. stenotomum accession, 12 tetraploid complex hybrids, and 13 diploid S. chacoense accessions. Four levels of NaCl (0, 40, 80, and 120 mM) were used. The cultivars, and the simple and complex hybrids were tested for salt tolerance at the vegetative stage in the nodal cutting bioassay. The thirteen S. chacoense accessions were tested for salt tolerance at the germination and early seedling growth stage, in a seedling bioassay. Eleven of these S. chacoense accessions were further tested at the vegetative stage, in the nodal cutting bioassay. There was a progressive decline in the morphological parameters measured, with increased salt levels, in the nodal cutting bioassay. The parameters were used collectively in ranking the different genotypes, averaged over three NaCl levels (40, 80, and 120 mM). Twenty potato cultivars, two clones of the simple hybrid S. chacoense $ times$ S. tuberosum, and one complex hybrid were all considered salt tolerant at the vegetative stage. Ranking of seven S. chacoense accessions was similar between early seedling growth and later vegetative stage. Two of these accessions were promising as sources of salt tolerance.

Thesis (Ph.D.)--University of Western Australia, 2006.

Barley is a resilient crop that performs better than other cereal plants under abiotic stress conditions, including salinity stress. The understanding of salinity tolerance in crops is a major milestone to increase yield in areas affected by soil salinity. In barley, some components of salinity tolerance have been elucidated, (e.g. HVP10, which is involved in tissue tolerance), yet little research has explored the discovery of other components contributing to salinity tolerance. In this PhD project, a forward genetics approach was used, whereby two barley populations were phenotyped under controlled and field conditions for salinity tolerance. The first population is a diversity panel of two-row European spring barley, and the second population is a nested association mapping barley population with wild donors from the Fertile Crescent. The use of non-destructive high-throughput experiments conducted under controlled conditions provided insight into the understudied shoot ion-independent component of salinity tolerance. In addition, the previously known association HvHKT1;5 was detected under controlled conditions. In parallel, the field experiments increased our understanding of new components of salinity tolerance, such as the maintenance of yield and yield-related traits under saline conditions. This strategy was successful with the identification of a locus on chromosome 2H (140-145 cM), where the allele from one of the wild donors of the nested association mapping population increased yield under saline conditions in the field. When re-evaluating lines homozygous at the 2H locus, ear length, ear number per plant, yield and harvest index were all significantly higher under saline conditions for the lines carrying the wild allele. Furthermore, another interesting locus on chromosome 7H that was responsive to salt treatment and co-localized with HVP1 was identified using the diversity panel population. Loci with known flowering genes were also shown to be involved in salinity tolerance. To conclude, this PhD project shed more light on the genetic mechanisms of salinity tolerance in barley, a knowledge that can benefit breeding programs and can be extended to other crops such as wheat.

No description available.

No description available.

Dryland salinity is a major and expanding threat to agricultural land in Australia. Animal production from forages grown on saline land is perhaps its most promising economic use. Glycophytic forage legumes have been evaluated under saline conditions mainly for agronomic characteristics and, to a lesser extent, for nutritive quality to animals. Plant growth and its nutritive quality are interrelated, but a decline in yield in response to salinity may be associated with effects on the chemical constituents of the plant since soil salinity affects plant metabolism. This research aimed to investigate changes in the components of yield and nutritive value of two legumes species. Lucerne ( Medicago sativa ) and Melilotus ( Melilotus albus ) were exposed to different levels of NaCl in the range of 0 to 110 mM NaCl. The research tested the hypothesis that the components of plant nutritive value are not as sensitive to salinity as shoot biomass production since the adaptive mechanisms of the plant lessen harmful effects of the salts. For both plant species, salinity decreased leaf and stem dry matter production, but increased leaf - to - stem ratio. In addition, salinity resulted in earlier flowering in Melilotus. Mineral composition was the most sensitive component of forage quality. Calculated sodium chloride concentrations were up to 125 g / kg DM in lucerne and 39 g / kg DM in Melilotus when irrigated with 110 mM NaCl. The concentrations of calcium and magnesium decreased in both species and approached the marginal range for animal production. Zinc concentration also decreased while potassium decreased in stems of lucerne only. The digestible organic matter ( DOMD ) in response to salinity varied between species. At the highest salt concentration, the whole shoot ( i.e., leaf and stem ) of lucerne decreased up to 4 percentage units while Melilotus increased by 6 percentage units. In lucerne, DOMD was influenced by a high concentration of soluble ash in leaf and stem and, in Melilotus, by an increase in the organic matter content of leaf and a reduction in lignin concentration in stem, which favoured higher digestibility. These results were supported by a histological study in which an increase in starch in Melilotus leaf, and a lower proportion of xylem in relation to parenchyma in stems, was measured. Crude protein concentration was not compromised and, in relation to Melilotus, coumarin concentration did not increase with salinity. In conclusion, the reduction in DM production of species with similar salt tolerance does not necessarily correspond to an equivalent reduction in nutritive value. This research represents the most detailed study into effects of salinity on glycophytic forage legumes. Results show that while some aspects of forage quality ( e.g., minerals composition and energy ) are strongly influenced by salinity, other aspects ( e.g., protein ) remain relatively unaffected. These findings have implications for development of productive grazing systems on saline agricultural land. / Thesis (Ph.D.)--School of Agriculture, Food and Wine, 2006.

Dryland salinity is a major and expanding threat to agricultural land in Australia. Animal production from forages grown on saline land is perhaps its most promising economic use. Glycophytic forage legumes have been evaluated under saline conditions mainly for agronomic characteristics and, to a lesser extent, for nutritive quality to animals. Plant growth and its nutritive quality are interrelated, but a decline in yield in response to salinity may be associated with effects on the chemical constituents of the plant since soil salinity affects plant metabolism. This research aimed to investigate changes in the components of yield and nutritive value of two legumes species. Lucerne ( Medicago sativa ) and Melilotus ( Melilotus albus ) were exposed to different levels of NaCl in the range of 0 to 110 mM NaCl. The research tested the hypothesis that the components of plant nutritive value are not as sensitive to salinity as shoot biomass production since the adaptive mechanisms of the plant lessen harmful effects of the salts. For both plant species, salinity decreased leaf and stem dry matter production, but increased leaf - to - stem ratio. In addition, salinity resulted in earlier flowering in Melilotus. Mineral composition was the most sensitive component of forage quality. Calculated sodium chloride concentrations were up to 125 g / kg DM in lucerne and 39 g / kg DM in Melilotus when irrigated with 110 mM NaCl. The concentrations of calcium and magnesium decreased in both species and approached the marginal range for animal production. Zinc concentration also decreased while potassium decreased in stems of lucerne only. The digestible organic matter ( DOMD ) in response to salinity varied between species. At the highest salt concentration, the whole shoot ( i.e., leaf and stem ) of lucerne decreased up to 4 percentage units while Melilotus increased by 6 percentage units. In lucerne, DOMD was influenced by a high concentration of soluble ash in leaf and stem and, in Melilotus, by an increase in the organic matter content of leaf and a reduction in lignin concentration in stem, which favoured higher digestibility. These results were supported by a histological study in which an increase in starch in Melilotus leaf, and a lower proportion of xylem in relation to parenchyma in stems, was measured. Crude protein concentration was not compromised and, in relation to Melilotus, coumarin concentration did not increase with salinity. In conclusion, the reduction in DM production of species with similar salt tolerance does not necessarily correspond to an equivalent reduction in nutritive value. This research represents the most detailed study into effects of salinity on glycophytic forage legumes. Results show that while some aspects of forage quality ( e.g., minerals composition and energy ) are strongly influenced by salinity, other aspects ( e.g., protein ) remain relatively unaffected. These findings have implications for development of productive grazing systems on saline agricultural land. / Thesis (Ph.D.)--School of Agriculture, Food and Wine, 2006.