Thesis (MScConsEcol (Conservation Ecology and Entomology))--Stellenbosch University, 2008. / The fynbos eco-region in the Western Cape lowlands has been extensively transformed by anthropogenic activities such as agriculture and urban development, as well as invasive alien plants. Elandsberg Private Nature Reserve and Farms lies on Swartland Alluvium Fynbos and has been largely fragmented by agricultural crop production, whilst Riverlands Nature Reserve together with the Pella Research Site, which lie on Atlantis Sand Fynbos, are under severe threat from the invasive woody alien Port Jackson Willow (Acacia saligna) which forms dense stands in the lands adjacent the nature reserve. Habitat transformation has resulted in subsequent loss of habitat and a reduction in habitat value for small mammal communities by creating a mosaic of optimal and sub-optimal habitats. The success of A. saligna is, in part, attributed to its production of copious amounts of small hard-shelled seeds and well established soil seed banks. In the first part of this study we investigated the effect of habitat transformation on resident small mammal communities by comparing small mammal species richness, diversity and abundance between natural and transformed habitats as well as between the two vegetation types. We also investigated the population demographics of the striped mouse (Rhabdomys pumilio) in the different habitats. Our study shows that whilst fragments of natural habitats, especially at Riverlands and Pella, may be playing an important role in harbouring rare and possibly endangered species, transformed habitats are also important as secondary habitats providing both cover as well as seasonal food resources. Reproduction and body condition in R. pumilio were dependent on seasonal changes such as availability of water and food resources in the habitats. The second part of this study was carried out to investigate the effect of alien plant invasion on the resident small mammal communities at Riverlands Nature Reserve and to determine the role of small mammals in the removal and predation of A. saligna seeds. Rodent species richness, diversity and abundance were very low in the A. saligna stands compared to the adjacent fynbos vegetation. The interaction of rodents with seeds (removal or predation) was dependent upon seed size and seed abundance in the habitats. Whilst rodents may play an important role in predator limitation in the acacias, it is also possible that they may be playing a significant role in dispersing the seeds of acacias into the fynbos. More work is required to fully understand the role small mammals play in seed predation, dispersal and limitation in alien invaded fynbos systems.

Hybrids are common, being found in about 25% of all plant species, but the isolating barriers which preserve species integrity are poorly studied. I investigated this question in the large New Zealand genus Celmisia Cass. (Asteraceae), which hybridises readily in cultivation, but wild hybrids are relatively rare. My study quantitatively tests four potential reproductive isolating barriers in 12 sympatric species of Celmisia found in the Craigieburn Range, inland Canterbury, New Zealand. I examined two potential prezygotic reproductive isolating barriers (flowering phenology and pollinator specialisation), and two potential postzygotic barriers (pre-dispersal seed predation and hybrid seed germination). I used null models to test whether Celmisia species had temporally segregated flowering times, and found that some Celmisia are temporally segregated and thus less likely to form hybrids. I used experimental pair-wise flowering arrays to observe insect visitation to six different Celmisia species pairs. While I found no difference in the overall pollinator community, several insect families showed preferences for some Celmisia species. Furthermore, I found that subtle floral character differences were driving these insect preferences. In particular, I found scape height to be positively associated with insect visitation with taller Celmisia being favoured over shorter species. Insect preferences did not translate into strong floral constancy, therefore indicating that Celmisia flower visitors are likely to be a weak barrier to hybridisation. I reared a range of insect seed predators from field-collected capitula of the hybrid C. x pseudolyallii and both parent species (C. lyallii and C. spectabilis). There was no overall difference in the number of seed-predators per capitulum between hybrid and parent Celmisia taxa. I collected and sowed seeds from three Celmisia hybrids and their parent species in order to test whether hybrids were less fertile than their parent species. I found no evidence to suggest that the seeds of hybrids had lower germination success than those of their parents. Overall I found evidence for only weak prezygotic reproductive isolation and no evidence for postzygotic isolation in the four barriers I examined in Celmisia.

A predação de sementes, juntamente com a dispersão, é um dos principais fatores determinantes no recrutamento de novos indivíduos. Espécies de plantas que oscilam na produção de frutos e sementes podem influenciar os predadores e dispersores, alterando a resposta destes em anos de baixa ou alta oferta de recursos. A araucária (Araucaria angustifolia) é o principal elemento da Floresta Ombrófila Mista e as suas sementes, os pinhões, além de amplamente consumidas pela fauna, são uma importante fonte de renda para muitas famílias. Dentre os animais, os roedores são os principais predadores dessas sementes. O objetivo de nosso estudo foi avaliar a relação entre a abundância dos roedores, a disponibilidade de frutos zoocóricos e de sementes da araucária. Avaliamos também se a disponibilidade de sementes influencia nas taxas de remoção e dispersão da araucária. Realizamos o estudo em oito áreas (quatro nativas e quatro de plantação com araucária) na Floresta Nacional de São Francisco de Paula, nordeste do Rio Grande do Sul. Amostramos os roedores durante cinco noites consecutivas em cada estação do ano (abr/outono, jul/inverno, out/primavera e jan/verão). Para avaliar a produção, acompanhamos mensalmente, entre jan/06 a jan/07, todos os indivíduos frutificando ao longo de duas transecções em cada área. Avaliamos também a predação e dispersão de sementes da araucária entre os meses de mai/06 a ago/06. Nossos resultados demonstraram que as sementes da araucária são um importante recurso nas áreas, principalmente no inverno, período em que houve uma queda acentuada na disponibilidade de outros frutos no solo. A abundância dos roedores variou entre as estações do ano, sendo maior no inverno e menor no verão. A remoção de sementes variou entre as áreas nativas e plantadas e entre os meses. Encontramos uma menor remoção nas áreas plantadas, locais de maior disponibilidade de pinhões no solo. A sobrevivência das sementes também foi maior nessas áreas. Nossos resultados indicaram um padrão de predação variando de acordo com a densidade, com uma correlação negativa entre a produção de sementes e a remoção. Nos locais de maior abundância de roedores e maior disponibilidade de sementes encontramos uma menor taxa de remoção, sugerindo uma possível saciação dos predadores. Essa estratégia pode ter conseqüências para o recrutamento e conservação da araucária, bem como para a dinâmica de predação e dispersão, caso não ocorra um manejo dos impactos associados a essa formação, como por exemplo, a coleta das sementes para comercialização e a presença de gado nas áreas. / Seed predation, as well as dispersion, is one of the determinant factors involved in the recruitments of new plant individuals. Plant species that oscillate in their fruit and seed production may influence their seed predators and dispersers, changing their responses in years with high or low resource offer. Araucaria angustifolia is the main element of the Araucaria Forest. Its seeds are largely consumed by the fauna as well as by people, being an important income source for many families. Among the animals that feed upon this seed, the rodents are the main predators. The aim of this study was to evaluate the relation between rodent abundance and fruit availability as well as Araucaria angustifolia seed availability. We also evaluated if the seed production influences the rate of predation and dispersion of araucaria seeds. We conducted the study in the National Forest of São Francisco de Paula, in Rio Grande do Sul state, southern Brazil. In this area, eight forested study sites were selected, four of them were covered by native vegetation (Mixed Ombrophilous Forest) and four were planted with Araucaria. We sampled the rodents at each site during five consecutive nights in each season (autumn, winter, spring, summer). To evaluate the amount of fruit production, we recorded all fruiting individuals of two transects of each area once a month between Jan/06 and Jan/07. We also evaluated the araucaria seed predation and dispersion between May/06 and Aug/06. Our results showed that Araucaria seeds are an important resource in the area, mainly in winter, on which availability of other fruits on the ground was lower. The rodent abundance changed between seasons, being higher during the winter and summer. Seed removal changed between native and planted sites as well as along months. We found low removal rates in planted areas, where overall seed availability on the ground was higher. Seed survival rate was also higher in these areas. Our results indicated a pattern of seed predation changing according to seed density, with a negative correlation between seed production and removal. In areas with higher rodent abundance, we detected lower removal rates, which suggests predator satiation. Such strategy might have consequences for recruitment and conservation of A. angustifolia, as well as for the predation and dispersion dynamic, in case of inadequate management of this species. Additional impacts on seed numbers, such as seed harvest for commercialization and the presence of cattle should be considered in management projects.

A predação de sementes, juntamente com a dispersão, é um dos principais fatores determinantes no recrutamento de novos indivíduos. Espécies de plantas que oscilam na produção de frutos e sementes podem influenciar os predadores e dispersores, alterando a resposta destes em anos de baixa ou alta oferta de recursos. A araucária (Araucaria angustifolia) é o principal elemento da Floresta Ombrófila Mista e as suas sementes, os pinhões, além de amplamente consumidas pela fauna, são uma importante fonte de renda para muitas famílias. Dentre os animais, os roedores são os principais predadores dessas sementes. O objetivo de nosso estudo foi avaliar a relação entre a abundância dos roedores, a disponibilidade de frutos zoocóricos e de sementes da araucária. Avaliamos também se a disponibilidade de sementes influencia nas taxas de remoção e dispersão da araucária. Realizamos o estudo em oito áreas (quatro nativas e quatro de plantação com araucária) na Floresta Nacional de São Francisco de Paula, nordeste do Rio Grande do Sul. Amostramos os roedores durante cinco noites consecutivas em cada estação do ano (abr/outono, jul/inverno, out/primavera e jan/verão). Para avaliar a produção, acompanhamos mensalmente, entre jan/06 a jan/07, todos os indivíduos frutificando ao longo de duas transecções em cada área. Avaliamos também a predação e dispersão de sementes da araucária entre os meses de mai/06 a ago/06. Nossos resultados demonstraram que as sementes da araucária são um importante recurso nas áreas, principalmente no inverno, período em que houve uma queda acentuada na disponibilidade de outros frutos no solo. A abundância dos roedores variou entre as estações do ano, sendo maior no inverno e menor no verão. A remoção de sementes variou entre as áreas nativas e plantadas e entre os meses. Encontramos uma menor remoção nas áreas plantadas, locais de maior disponibilidade de pinhões no solo. A sobrevivência das sementes também foi maior nessas áreas. Nossos resultados indicaram um padrão de predação variando de acordo com a densidade, com uma correlação negativa entre a produção de sementes e a remoção. Nos locais de maior abundância de roedores e maior disponibilidade de sementes encontramos uma menor taxa de remoção, sugerindo uma possível saciação dos predadores. Essa estratégia pode ter conseqüências para o recrutamento e conservação da araucária, bem como para a dinâmica de predação e dispersão, caso não ocorra um manejo dos impactos associados a essa formação, como por exemplo, a coleta das sementes para comercialização e a presença de gado nas áreas. / Seed predation, as well as dispersion, is one of the determinant factors involved in the recruitments of new plant individuals. Plant species that oscillate in their fruit and seed production may influence their seed predators and dispersers, changing their responses in years with high or low resource offer. Araucaria angustifolia is the main element of the Araucaria Forest. Its seeds are largely consumed by the fauna as well as by people, being an important income source for many families. Among the animals that feed upon this seed, the rodents are the main predators. The aim of this study was to evaluate the relation between rodent abundance and fruit availability as well as Araucaria angustifolia seed availability. We also evaluated if the seed production influences the rate of predation and dispersion of araucaria seeds. We conducted the study in the National Forest of São Francisco de Paula, in Rio Grande do Sul state, southern Brazil. In this area, eight forested study sites were selected, four of them were covered by native vegetation (Mixed Ombrophilous Forest) and four were planted with Araucaria. We sampled the rodents at each site during five consecutive nights in each season (autumn, winter, spring, summer). To evaluate the amount of fruit production, we recorded all fruiting individuals of two transects of each area once a month between Jan/06 and Jan/07. We also evaluated the araucaria seed predation and dispersion between May/06 and Aug/06. Our results showed that Araucaria seeds are an important resource in the area, mainly in winter, on which availability of other fruits on the ground was lower. The rodent abundance changed between seasons, being higher during the winter and summer. Seed removal changed between native and planted sites as well as along months. We found low removal rates in planted areas, where overall seed availability on the ground was higher. Seed survival rate was also higher in these areas. Our results indicated a pattern of seed predation changing according to seed density, with a negative correlation between seed production and removal. In areas with higher rodent abundance, we detected lower removal rates, which suggests predator satiation. Such strategy might have consequences for recruitment and conservation of A. angustifolia, as well as for the predation and dispersion dynamic, in case of inadequate management of this species. Additional impacts on seed numbers, such as seed harvest for commercialization and the presence of cattle should be considered in management projects.

Submitted by Maria de Lourdes Mariano (lmariano@ufscar.br) on 2017-01-12T17:18:37Z No. of bitstreams: 1 SILVA_Vanessa Mariano_2015.pdf: 1028554 bytes, checksum: b983689081dffe8a634db905adc7c152 (MD5) / Approved for entry into archive by Maria de Lourdes Mariano (lmariano@ufscar.br) on 2017-01-12T17:19:59Z (GMT) No. of bitstreams: 1 SILVA_Vanessa Mariano_2015.pdf: 1028554 bytes, checksum: b983689081dffe8a634db905adc7c152 (MD5) / Approved for entry into archive by Maria de Lourdes Mariano (lmariano@ufscar.br) on 2017-01-12T17:20:07Z (GMT) No. of bitstreams: 1 SILVA_Vanessa Mariano_2015.pdf: 1028554 bytes, checksum: b983689081dffe8a634db905adc7c152 (MD5) / Made available in DSpace on 2017-01-12T17:20:15Z (GMT). No. of bitstreams: 1 SILVA_Vanessa Mariano_2015.pdf: 1028554 bytes, checksum: b983689081dffe8a634db905adc7c152 (MD5) Previous issue date: 2015-12-18 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Plant recruitment can be constrained by seed and establishment limitation and these processes can affect community species composition and diversity. In Neotropical savannas the relative importance of these processes for plant regeneration are unclear because of the scarcity of studies. The aim of this study was to evaluate the importance of seed and establishment limitation to plant recruitment on cerrado savanna in Itirapina, southeast Brazil (22°12´S, 47°52´W). We spread 49 seed traps in a 0.64-ha plot and monitored seed rain monthly for a year, and also conducted seed addition experiments. Seed production presented a peak in rainy season, with zoochoric and anemochoric species producing seeds mainly in rainy and dry season respectively. Our study indicates a strong influence of seed limitation on recruitment, with most seed rain species presenting seed limitation values higher than 65%. Only 23% of woody species in the plot had at least one seed trapped. Seed and source limitation was negative related to adult abundance, indicating that the increase of seed sources enhances the number of suitable sites reached by seeds. There was no relation between seed, source and dispersal limitation and plant life form, dispersal syndrome and seed mass. Seed addition was unable to increase seedling regeneration in the four species tested, which presented high values of establishment limitation. Our study shows that Neotropical savanna plants recruitment is restricted by seed and establishment limitation and highlights the role of seed production and dispersal to colonization of new sites. / O recrutamento de plantas pode ser restringido pelas limitações de sementes e no estabelecimento, os quais afetam a composição e diversidade de uma comunidade. Em savanas neotropicais a importância relativa desses processos ainda não é clara em decorrência da falta de estudos. O objetivo desse estudo foi avaliar a importância das limitações de sementes e no estabelecimento para o recrutamento de uma comunidade de cerrado no sudeste do Brasil. Foram posicionados 49 coletores de sementes em uma parcela de 0,64 ha e a chuva de sementes foi monitorada mensalmente durante um ano. Além disso, foram realizados experimentos de adição de sementes. A produção de sementes apresentou um pico durante a estação chuvosa, sendo que as espécies zoocóricas produziram principalmente durante a estação chuvosa e as anomocóricas, durante a estação seca. Houve uma influência significativa da limitação de sementes no recrutamento, sendo que a maioria das espécies presentes na chuva de sementes apresentou valores dessa limitação maiores que 65%. Apenas 23% das espécies arbustivo-arbóreas presentes na parcela tiveram, pelo menos, uma semente coletada. As limitações de sementes e na fonte apresentaram uma relação negativa com o número de adultos, indicando que o aumento de fontes de sementes eleva o número de locais atingidos por sementes. Não houve relação entre limitações de sementes, na fonte e na dispersão com forma de vida, síndrome de dispersão e peso da semente. A adição de sementes não aumentou a regeneração de plântulas das quatro espécies testadas, as quais apresentaram valores elevados de limitação no estabelecimento. O presente estudo indica que o recrutamento de plantas de savanas neotropicais é restringido pelas limitações de sementes e no estabelecimento e ressalta a importância da produção e dispersão de sementes para a colonização de novos locais.

A predação de sementes, juntamente com a dispersão, é um dos principais fatores determinantes no recrutamento de novos indivíduos. Espécies de plantas que oscilam na produção de frutos e sementes podem influenciar os predadores e dispersores, alterando a resposta destes em anos de baixa ou alta oferta de recursos. A araucária (Araucaria angustifolia) é o principal elemento da Floresta Ombrófila Mista e as suas sementes, os pinhões, além de amplamente consumidas pela fauna, são uma importante fonte de renda para muitas famílias. Dentre os animais, os roedores são os principais predadores dessas sementes. O objetivo de nosso estudo foi avaliar a relação entre a abundância dos roedores, a disponibilidade de frutos zoocóricos e de sementes da araucária. Avaliamos também se a disponibilidade de sementes influencia nas taxas de remoção e dispersão da araucária. Realizamos o estudo em oito áreas (quatro nativas e quatro de plantação com araucária) na Floresta Nacional de São Francisco de Paula, nordeste do Rio Grande do Sul. Amostramos os roedores durante cinco noites consecutivas em cada estação do ano (abr/outono, jul/inverno, out/primavera e jan/verão). Para avaliar a produção, acompanhamos mensalmente, entre jan/06 a jan/07, todos os indivíduos frutificando ao longo de duas transecções em cada área. Avaliamos também a predação e dispersão de sementes da araucária entre os meses de mai/06 a ago/06. Nossos resultados demonstraram que as sementes da araucária são um importante recurso nas áreas, principalmente no inverno, período em que houve uma queda acentuada na disponibilidade de outros frutos no solo. A abundância dos roedores variou entre as estações do ano, sendo maior no inverno e menor no verão. A remoção de sementes variou entre as áreas nativas e plantadas e entre os meses. Encontramos uma menor remoção nas áreas plantadas, locais de maior disponibilidade de pinhões no solo. A sobrevivência das sementes também foi maior nessas áreas. Nossos resultados indicaram um padrão de predação variando de acordo com a densidade, com uma correlação negativa entre a produção de sementes e a remoção. Nos locais de maior abundância de roedores e maior disponibilidade de sementes encontramos uma menor taxa de remoção, sugerindo uma possível saciação dos predadores. Essa estratégia pode ter conseqüências para o recrutamento e conservação da araucária, bem como para a dinâmica de predação e dispersão, caso não ocorra um manejo dos impactos associados a essa formação, como por exemplo, a coleta das sementes para comercialização e a presença de gado nas áreas. / Seed predation, as well as dispersion, is one of the determinant factors involved in the recruitments of new plant individuals. Plant species that oscillate in their fruit and seed production may influence their seed predators and dispersers, changing their responses in years with high or low resource offer. Araucaria angustifolia is the main element of the Araucaria Forest. Its seeds are largely consumed by the fauna as well as by people, being an important income source for many families. Among the animals that feed upon this seed, the rodents are the main predators. The aim of this study was to evaluate the relation between rodent abundance and fruit availability as well as Araucaria angustifolia seed availability. We also evaluated if the seed production influences the rate of predation and dispersion of araucaria seeds. We conducted the study in the National Forest of São Francisco de Paula, in Rio Grande do Sul state, southern Brazil. In this area, eight forested study sites were selected, four of them were covered by native vegetation (Mixed Ombrophilous Forest) and four were planted with Araucaria. We sampled the rodents at each site during five consecutive nights in each season (autumn, winter, spring, summer). To evaluate the amount of fruit production, we recorded all fruiting individuals of two transects of each area once a month between Jan/06 and Jan/07. We also evaluated the araucaria seed predation and dispersion between May/06 and Aug/06. Our results showed that Araucaria seeds are an important resource in the area, mainly in winter, on which availability of other fruits on the ground was lower. The rodent abundance changed between seasons, being higher during the winter and summer. Seed removal changed between native and planted sites as well as along months. We found low removal rates in planted areas, where overall seed availability on the ground was higher. Seed survival rate was also higher in these areas. Our results indicated a pattern of seed predation changing according to seed density, with a negative correlation between seed production and removal. In areas with higher rodent abundance, we detected lower removal rates, which suggests predator satiation. Such strategy might have consequences for recruitment and conservation of A. angustifolia, as well as for the predation and dispersion dynamic, in case of inadequate management of this species. Additional impacts on seed numbers, such as seed harvest for commercialization and the presence of cattle should be considered in management projects.

Orientador: João Vasconcellos Neto / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-18T12:02:14Z (GMT). No. of bitstreams: 1 Cortinoz_JanainaRosa_M.pdf: 4578182 bytes, checksum: 437846becfcb9944124c33d6a5acf722 (MD5) Previous issue date: 2011 / Resumo: Plantas com sementes grandes, como palmeiras, são mais afetadas em áreas defaunadas. A redução de dispersores de sementes resulta em maior acumulo de sementes sob a planta-mãe, onde a predação e mais intensa. Roedores e besouros são os principais predadores de sementes de palmeiras e podem interagir entre si, competindo por sementes, ou com roedores predando larvas de besouros ao consumirem sementes previamente infestadas. Ainda, besouros podem ter suas larvas atacadas por vespas parasitóides. Assim, o objetivo deste trabalho foi investigar os efeitos da dispersão e predação de sementes da palmeira Syagrus romanzoffiana por vertebrados sobre a predação de sementes por besouros e o ataque de parasitóides em locais que diferem entre si quanto a presença de vertebrados dispersores e/ou predadores de sementes. As coletas foram realizadas na Serra do Japi em Jundiaí, Reserva Municipal Mata de Santa Genebra, Bosque dos Jequitibás e no campus da Universidade Estadual de Campinas, em Campinas. A predação de sementes por vertebrados e besouros foi comparada entre as áreas. Dados a respeito da fenologia reprodutiva da palmeira e taxas mensais de remoção de frutos por vertebrados e predação de sementes pré e pós-dispersão por besouros foram coletados para avaliar como as interações ocorrem ao longo do período de frutificação da planta. A predação de sementes por besouros foi negativamente relacionada a predação por vertebrados, sendo maior em áreas onde vertebrados predadores de sementes estão ausentes ou em baixas densidades, como Unicamp e Mata de Santa Genebra. A predação por bruquídeos foi menor na presença de esquilos na Serra do Japi e cutias no Bosque dos Jequitibás, mas a predação por curculionídeos foi menor apenas na Serra do Japi. O que pode indicar que o consumo de sementes por vertebrados deve reduzir a quantidade de sementes disponíveis para besouros bruquídeos, e/ou que ha predação intraguilda de larvas de bruquídeos por esquilos e cutias, mas que cutias, provavelmente rejeitam sementes atacadas por curculionídeos. A produção de flores e frutos imaturos foi semelhante entre as áreas, mas o encontro de frutos maduros foi menor na Serra do Japi e com o pico de produção deslocado em relação as outras áreas, possivelmente devido a alta predação de sementes por esquilos nesse local. A predação de sementes pré-dispersão pelo besouro Revena rubiginosa (Curculionidae) ocorreu em todos os meses e o período de oviposição dos besouros não apresentou relação com a disponibilidade de frutos imaturos. Besouros das famílias Bruchidae e Scolytidae predaram as sementes pós-dispersão, que foi maior onde a remoção de sementes por vertebrados foi menor e não esteve relacionada a disponibilidade de frutos maduros e a predação pré-dispersão. Larvas de R. rubiginosa e de bruquideos foram atacadas por vespas parasitoides da família Braconidae na Unicamp e Mata de Santa Genebra. O período de oviposição das vespas não apresentou relação com as taxas de predação dos besouros, e sim com o clima. Dessa forma, como não foram encontrados indícios que sementes sejam recursos limitantes para besouros, a predação de sementes por esses insetos parece ser limitada pela remoção e predação de sementes por vertebrados. Na ausência de vertebrados dispersores e/ou predadores de sementes que controlem a predação por besouros o ataque de parasitóides as larvas e maior / Abstract: Plants with large seeds such as palm trees are more affected in defaunated areas. The absence of seed dispersers results in a higher accumulation of seeds beneath the parent plant, where seed predation is more intense. Rodents and beetles are major predators of palm seed and can interact with each other, competing for seeds. Rodents can also prey on beetles' larvae when consume seed previously infested, and beetles may have their larvae attacked by parasitoid wasps. Thus, the aim of this work was investigate the effects of seed dispersal and predation of the palm Syagrus romanzoffiana by vertebrates on seed predation by beetles and the parasitoid attack in areas that differ with regard to the presence of vertebrate seed dispersers and/or predators. Data was collected at four sites. One of them at Jundiai, at Serra do Japi, and other three at Campinas, SP: Mata de Santa Genebra Municipal Reserve, Bosque dos Jequitibas and Campinas State University's Campus. Seed predation by vertebrates and beetles was compared between areas. Data regarding to the reproductive phenology and monthly rates of pre- and post-dispersal seed predation by beetles were also collected to assess how the interaction occurs throughout of the fruiting season of the plant. Seed predation by beetles was negatively related to seed predation by vertebrates, being higher in areas where vertebrate seed predators are absent or present in low densities, as at Unicamp and Mata de Santa Genebra. Seed predation by bruchids beetles was lower in the presence of the squirrels at Serra do Japi and agoutis at Bosque dos Jequitibas, but seed predation by weevils was lower only at Serra do Japi. These findings may indicate that the consumption of S. romanzoffiana seeds by vertebrates should reduce the amount of seeds available for bruchids beetles, and/or there are intraguild predation on larvae of bruchids by squirrels and agoutis, but agoutis probably reject seeds infested by weevils larvae. The flower and unripe fruit production was similar in all areas, but ripe fruit production was smaller at Serra do Japi, with a displaced peak when compared to the other sites, probably due to the high seed predation by squirrel in this area. Pre-dispersal seed predation by the beetle Revena rubiginosa (Curculionidae) occurred in all months and the period of beetles' oviposition was not related to the unripe fruits availability. Bruchids and scolytids beetles prey on post dispersed seeds, which was greater where the seed removal by vertebrate was lower. Also the post dispersal seed predation was not related to the ripe fruits availability neither to the pre dispersal seed predation. Larvae of R. rubiginosa and bruchids were attacked by braconids wasps at Unicamp and Mata de Santa Genebra. The oviposition by parasitoids was not related to the rates of seed predation by beetles, but was related to the climate. Thus, in the lack of evidence that seeds are a restrict resource, the seed predation by these insects seems to be limited by seed removal and predation by vertebrates. In the absence of vertebrate seed dispersers and/or predators, which control the seed predation by beetles, the attack by parasitoids wasps is higher / Mestrado / Ecologia / Mestre em Ecologia

The Effects of Tropical Forest Management on Biodiversity and Ecosystem Functioning Eleanor M. Slade 1. Between 35 % and 50 % of all closed-canopy tropical forest has been lost, and the rate of deforestation continues to increase throughout the tropics. Despite a wealth of literature on the effects of tropical forest disturbance on the diversity and composition of a variety of taxa, there is still no clear consensus on the value of disturbed forests for biodiversity. 2. If forest management practises are to be sustainable in the long-term they should maintain both biodiversity and ecosystem functioning (the interactions and processes of the ecosystem), as well as a timber harvest. However, few studies have investigated the extent to which ecosystem functioning is reduced in logged forests. The effects of different logging intensities on a variety of taxa, and the ecosystem processes with which they are associated, were assessed in the Danum Valley Conservation Area in Sabah (Malaysian Borneo). 3. Even under high logging intensities, the forests of Sabah appear to have been managed in a way that maintains timber yields in the short-term. However, other aspects of forest structure had been affected, which could have important consequences ecologically, and for the long-term sustainability of timber harvests. 4. Combining field studies with manipulative experiments allows assessment of the impacts of species changes associated with habitat modification on measures of ecosystem functioning. Dung beetle (Coleoptera: Scarabaeidae: Scarabaeinae) functional group richness and composition were manipulated in a series of field experiments. Certain functional groups and species were found to have a greater impact on ecosystem functioning than others; nevertheless a full complement of species was needed to maintain full ecosystem functioning. 5. Dung beetles appeared to be relatively robust to changes in forest structure associated with selective logging, but species richness was reduced with high-intensity logging. There was a corresponding decrease in ecosystem functioning (dung and seed removal) with a decrease in species richness, and a decrease in the biomass of large nocturnal tunnellers, suggesting that although some species are dominant, rare species are also needed to preserve full ecosystem functioning. 6. A complex interaction between birds and ants resulted in reduced herbivory of seedlings of the important timber tree, Parashorea malaanonan, in some instances. However, this interaction was not affected by either selective or high intensity logging. Seedfall of P. malaanonan, was reduced in logged forest compared to primary forest. Despite insect seed predation being higher in primary forest, there was still successful recruitment during a non-mast year. Parasitism of insect-predated seeds was found to be inversely density dependent, and was higher in logged forest where seed predation was lower. 7. The results of this thesis suggest that the forests of Sabah appear to be being logged under a management system that is compatible with sustainable timber management, but not necessarily sustainable forest management. Low intensity selective logging seems to preserve much of the original forest structure, biodiversity and ecosystem functioning compared to logging at higher intensities. However, ecosystem processes were variable in their response to logging, suggesting that management decisions should be based on the consideration of multiple taxa and processes.

<p>Plant-animal interactions are affected by both abundance and distribution of interacting species and the community context in which they occur. However, the relative importance of these factors is poorly known. I examined the effects of predator host range, environmental factors, host plant populations, plant traits and fruit abortion on the intensity of pre-dispersal seed predation in 46 host populations of the perennial herb <i>Lathyrus vernus</i>. I recorded damage by beetle pre-dispersal seed predators, mainly <i>Apion opeticum </i>and <i>Bruchus atomarius</i> with different host ranges on <i>L. vernus</i> as well as on two additional host plants. Local seed predator population size was mainly influenced by plant population size, current seed production and beetle population size in the previous year, but was not strongly affected by connectivity. The monophagous seed predator was less abundant and had lower densities than the oligophagous. Both predator species had a strong ability to track fluctuations in seed production; intensity of predation increased with relative increases in seed production. Oligophagous predation on<i> L. vernus i</i>ncreased with the abundance of alternative hosts, but presence of<i> L. vernus</i> did not affect predation on alternative hosts. Abundances and trait preferences differed among three co-occurring seed predators, but were also associated with the abundance of the other species. Overall, seed predation influenced selection on flower number. I found clear indications of seed predator offence but no obvious plant defence. The pattern of fruit abortion was associated with reduced plant fitness since the seed predator had an advanced ability to locate fruits with high probability of retention. Taken together, different factors influencing abundance of the seed predator species, different preferences, and context dependent trait selection are likely to result in complex spatio-temporal variation in overall seed losses and trait selection in the common host plant.</p>

<p>I combined comparative and experimental studies in the field and in the greenhouse to examine factors influencing reproductive success in two insect-pollinated herbs, the tristylous, selfincompatible perennial <i>Lythrum salicaria</i> and the self-compatible, biennial <i>Pedicularis palustris</i>. More specifically, I explored (i) the effects of plant population characteristics on the intensity and outcome of interactions with pollinators and seed predators, (ii) whether flower morphology affects duration of stigma receptivity, and (iii) whether damage-induced reduction in floral display reduces pollinator visitation and increases pollen limitation.</p><p>As predicted, the rate of pollinator visitation tended to increase and the number of flowers probed per plant tended to decrease with increasing population size in <i>L. salicaria</i>, but these relationships only approached statistical significance. By taking advantage of the pollen size polymorphism that is typical of many heterostylous plants, I could show that the number of compatible pollen grains received increased with population size, and that this was associated with a reduction in pollen limitation and increased seed output per flower. The deposition of high numbers of incompatible conspecific and heterospecific pollen grains did not appear to reduce seed set.</p><p>In <i>P. palustris</i>, fruit set and seed predation varied markedly among populations and years, but this variation could only partly be explained by variation in population size. Fruit set was positively related to population size, and seed predation was negatively related to population size, in one of three years. Similarly, the level of pollen limitation, which was quantified in two years, varied among populations, but was not related to population size, density or isolation.</p><p>In <i>L. salicaria</i>, both the duration of stigma receptivity and the effect of prior self-pollination on seed output varied among style morphs. These differences may contribute to morph-specific differences in pollen limitation and seed production documented in the field.</p><p>The results of a field experiment demonstrated that damage to the shoot apex may markedly reduce fruit production in <i>L. salicaria</i>, and suggested that this is mainly because damage reduces flower number. I found no evidence that a reduced floral display increased the severity of pollen limitation.</p><p>Taken together, the results show that interactions with both pollinators and herbivores may markedly affect reproductive output in the two plant species, and that the intensity of both mutualistic and antagonistic interactions vary considerably in space and time. Moreover, they are consistent with the hypothesis that pollination success should depend less on population size in selfcompatible than in self-incompatible plants.</p>