by Sik Lok Lam. / The "2" in the title is subscript. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 184-197). / ABSTRACT --- p.iii / ACKNOWLEDGEMENTS --- p.v / Chapter CHAPTER ONE: --- LITERATURE SURVEY OF SEQUENCE-SPECIFIC LOCAL STRUCTURAL VARIATIONS IN DEOXYRIBONUCLEIC ACID MOLECULES --- p.1 / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- General Review of DNA --- p.1 / Chapter 1.2.1 --- "Nomenclature, Symbols and Atomic Numbering Scheme of DNA" --- p.2 / Chapter 1.2.2 --- Conformations of DNAs --- p.6 / Chapter 1.2.3 --- Helix-to-Random-Coil Transition --- p.9 / Chapter 1.3 --- Sequence-Specific Local Structural Studies --- p.11 / Chapter 1.4 --- Purpose of This Work --- p.14 / Chapter CHAPTER TWO: --- DETERMINATION OF STRUCTURES OF SOLUTION DNA MOLECULES --- p.17 / Chapter 2.1 --- Introduction --- p.17 / Chapter 2.2 --- Optimization of Conditions --- p.17 / Chapter 2.3 --- Resonance Assignments --- p.19 / Chapter 2.4 --- Extraction of Structural Constraints --- p.22 / Chapter 2.4.1 --- Interproton Distances --- p.23 / Chapter 2.4.2 --- Endocyclic Sugar Torsion Angles --- p.25 / Chapter 2.4.3 --- Phosphate Backbone Torsion Angles --- p.29 / Chapter 2.4.4 --- Hydrogen Bonds --- p.31 / Chapter 2.5 --- Structural Refinement --- p.31 / Chapter CHAPTER THREE: --- SIGNIFICANCE OF DIFFERENT TYPES OF STRUCTURAL CONSTRAINTS IN STRUCTURAL REFINEMENT PROCESS --- p.35 / Chapter 3.1 --- Introduction --- p.35 / Chapter 3.2 --- Experimental --- p.36 / Chapter 3.2.1 --- DNA Model Building --- p.36 / Chapter 3.2.2 --- Generation of Structural Constraints --- p.37 / Chapter 3.2.3 --- Structural Refinement --- p.40 / Chapter 3.3 --- Results and Discussion --- p.41 / Chapter 3.3.1 --- Endocyclic Sugar Torsion Angle Constraints --- p.45 / Chapter 3.3.2 --- Phosphate Backbone Torsion Angle Constraints --- p.49 / Chapter 3.3.3 --- Hydrogen Bond Constraints --- p.50 / Chapter 3.4 --- Summary --- p.50 / Chapter CHAPTER FOUR: --- EFFECTS OF DIFFERENT VARIABLES IN THE RESTRAINED MOLECULAR DYNAMICS PROCESS --- p.52 / Chapter 4.1 --- Introduction --- p.52 / Chapter 4.2 --- Experimental --- p.53 / Chapter 4.3 --- Results and Discussion --- p.55 / Chapter 4.3.1 --- Variables in the Temperature Profile --- p.58 / Chapter 4.3.2 --- Variables in the Force Constant Profile --- p.62 / Chapter 4.4 --- Summary --- p.65 / Chapter CHAPTER FIVE: --- THE J-COUPLING RESTRAINED MOLECULAR MECHANICS PROTOCOL - AN EFFICIENT AND RELIABLE ALTERNATIVE IN DERIVING ENDOCYCLIC SUGAR TORSION ANGLE CONSTRAINTS --- p.66 / Chapter 5.1 --- Introduction --- p.66 / Chapter 5.2 --- Methodology --- p.71 / Chapter 5.2.1 --- "Establishment of the Correlation of 3J1'2, withvi" --- p.71 / Chapter 5.2.2 --- Sample Preparation --- p.73 / Chapter 5.2.3 --- NMR Analysis --- p.73 / Chapter 5.2.4 --- Theoretical Testing of the Protocol --- p.74 / Chapter 5.2.5 --- Experimental Testing of the Protocol --- p.75 / Chapter 5.3 --- Results and Discussion --- p.76 / Chapter 5.3.1 --- Selection of the Appropriate JrMM-derived Torsion Angles --- p.85 / Chapter 5.3.2 --- Theoretical Testing of the Protocol --- p.88 / Chapter 5.3.3 --- Experimental Testing of the Protocol --- p.93 / Chapter 5.4 --- Summary --- p.98 / Chapter CHAPTER SIX: --- HETERONUCLEAR SINGLE QUANTUM COHERENCE DERIVED BACKBONE TORSION ANGLE CONSTRAINTS --- p.99 / Chapter 6.1 --- Introduction --- p.99 / Chapter 6.2 --- Experimental --- p.102 / Chapter 6.3 --- Results and Discussion --- p.103 / Chapter 6.3.1 --- Determination of the Backbone Torsion Angles β and E --- p.103 / Chapter 6.3.2 --- Error Estimation on 3JC4'p- and 3JH3'p-derived E --- p.109 / Chapter 6.4 --- Summary --- p.110 / Chapter CHAPTER SEVEN: --- SOLUTION STRUCTURES OF d(CGXX，CG)2 AND d(CAXX´ةTG)2 --- p.111 / Chapter 7.1 --- Introduction --- p.111 / Chapter 7.2 --- Experimental --- p.111 / Chapter 7.2.1 --- Sample Preparation --- p.112 / Chapter 7.2.2 --- Resonance Assignment --- p.112 / Chapter 7.2.3 --- Melting Profile Study --- p.112 / Chapter 7.2.4 --- Extraction of Structural Constraints --- p.113 / Chapter 7.2.5 --- Structural Refinement --- p.115 / Chapter 7.2.6 --- Structural Parameter Analysis --- p.116 / Chapter 7.3 --- Results and Discussion --- p.116 / Chapter 7.3.1 --- Melting Profile Study --- p.117 / Chapter 7.3.2 --- Structural Constraints --- p.120 / Chapter 7.3.3 --- Structural Refinement --- p.129 / Chapter 7.3.4 --- Structural Features --- p.135 / Chapter CHAPTER EIGHT: --- SEQUENCE-SPECIFIC LOCAL STRUCTURAL STUDY --- p.156 / Chapter 8.1 --- Introduction --- p.156 / Chapter 8.2 --- Predictions from the Calladine's Rules --- p.156 / Chapter 8.3 --- Predictions from Olson's Base-Pair Morphology Dependent Clash Function --- p.160 / Chapter 8.4 --- Re-formulation of Calladine's Idea and its Relationship to Sequence-Specific Local Structural Function ΣLS --- p.163 / Chapter 8.4.1 --- Sequence-Specific Base-Pair Geometry Analysis --- p.164 / Chapter 8.4.2 --- Sequence-Specific Base-Pair Step Geometry Analysis --- p.166 / Chapter 8.4.3 --- Sequence-Specific Local Structural Function ΣLS --- p.167 / Chapter 8.5 --- Summary --- p.173 / Chapter CHAPTER NINE: --- CONCLUSIONS AND FURTHER WORK --- p.174 / APPENDIX I The Base Proton Regions of the lH NMR Spectra of the Hexamers --- p.177 / APPENDIX II 2D NOESY Spectra (Tm = 200 ms) of the Hexamers --- p.178 / "APPENDIX III The H1'-H27H2"" Regions of the DQF-COSY Spectra of the Hexamers" --- p.180 / APPENDIX IV The C4'-H4' Regions of the HSQC Spectra of the Hexamers --- p.182 / REFERENCES --- p.184

Diss. (sammanfattning) Uppsala : Uppsala universitet, 2005. / Härtill 6 uppsatser.

by Tsui Kwok-wing. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 188-216). / ACKNOWLEDGEMENTS --- p.ii / ABSTRACT --- p.iii / TABLE OF CONTENTS --- p.v / ABBREVIATIONS --- p.ix / Chapter CHAPTER 1 --- INTRODUCTION / Chapter 1.1 --- General introduction --- p.1 / Chapter 1.2 --- Human genome project --- p.5 / Chapter 1.3 --- Organization of human genome --- p.7 / Chapter 1.4 --- Adult human heart cDNA library --- p.9 / Chapter 1.5 --- Gene expression in adult human heart --- p.10 / Chapter 1.6 --- Polymerase chain reaction --- p.12 / Chapter 1.7 --- Purification of PCR products --- p.15 / Chapter 1.8 --- Automated DNA sequencing --- p.17 / Chapter 1.9 --- Sequence analysis by electronic mail server --- p.21 / Chapter 1.10 --- Effects of agar and agarose on Vent´ёØ and Taq DNA polymerases --- p.23 / Chapter 1.11 --- Transcription factors and zinc finger proteins --- p.25 / Chapter 1.12 --- LIM domain --- p.28 / Chapter 1.13 --- Cysteine-rich intestinal protein --- p.30 / Chapter CHAPTER 2 --- MATERIALS AND METHODS / Chapter 2.1 --- Plating out the adult human heart cDNA library --- p.32 / Chapter 2.2 --- Amplification by polymerase chain reaction --- p.33 / Chapter 2.3 --- Purification of the PCR products by Millipore filters --- p.35 / Chapter 2.4 --- Elimination of the purification of the PCR products before sequencing --- p.36 / Chapter 2.5 --- Cycle sequencing --- p.37 / Chapter 2.6 --- Unicycle sequencing --- p.38 / Chapter 2.7 --- Sequencing by T7 polymerase --- p.39 / Chapter 2.8 --- Gel electrophoresis in the automated A.L.F. sequencer --- p.41 / Chapter 2.9 --- Sequence analysis by commercially available softwares --- p.42 / Chapter 2.10 --- Sequence analysis through electronic mail server --- p.44 / Chapter 2.11 --- Database for storing the result of each clone --- p.46 / Chapter 2.12 --- Effects of agar and agarose on Vent´ёØ and Taq DNA polymerase --- p.47 / Chapter 2.13 --- Mini-preparation of plasmid DNA --- p.50 / Chapter 2.14 --- Large scale preparation of plasmid DNA --- p.51 / Chapter 2.15 --- Cloning the human cysteine rich heart protein (hCRHP) into the pAED4 vector --- p.53 / Chapter 2.16 --- Expression of hCRHP in E coli --- p.56 / Chapter 2.17 --- Northern hybridization --- p.58 / Chapter 2.18 --- Partial protein sequencing of hCRHP --- p.59 / Chapter CHAPTER 3 --- RESULTS / Chapter 3.1 --- The sequencing results of adult human heart cDNA clones --- p.60 / Chapter 3.2 --- Accuracy of sequencing results --- p.63 / Chapter 3.3 --- Catalogues of genes expressed in the adult human heart --- p.65 / Chapter 3.4 --- Effects of agar and agarose on Vent´ёØ and Taq DNA polymerases --- p.94 / Chapter 3.5 --- Elimination of the purification of the PCR products before sequencing --- p.102 / Chapter 3.6 --- Sequence analysis of hCRHP --- p.104 / Chapter 3.7 --- Northern hybridization of hCRHP --- p.109 / Chapter 3.8 --- Expression of hCRHP in E. coli --- p.112 / Chapter CHAPTER 4 --- DISCUSSION / Chapter 4.1 --- Random sequencing of adult human heart cDNA clones --- p.118 / Chapter 4.2 --- Catalogues of genes expressed in the adult human heart --- p.130 / Chapter 4.3 --- Gene expression in the adult human heart --- p.137 / Chapter 4.4 --- Importance of nonhuman matches --- p.170 / Chapter 4.5 --- Effects of agar and agarose on Vent´ёØ and Taq DNA polymerases --- p.177 / Chapter 4.6 --- Elimination of the purification of the PCR products before sequencing --- p.180 / Chapter 4.7 --- The possible role of CRIP and hCRHP --- p.184 / Chapter 4.8 --- Future prospect --- p.186 / REFERENCE --- p.188

by Agnes, Lo Shuk Yee. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves [27-33] (2nd gp.)). / Chapter PART 1: --- Human Heart cDNA Library Sequencing / Chapter A) --- Introduction of human heart cDNA library sequencing / Chapter A.1 --- Human genome project / Chapter A.2 --- The aim of human genome project / Chapter A.3 --- Automatic sequencing / Chapter A.4 --- Cycle sequencing reaction / Chapter A.5 --- Human heart cDNA library sequencing project / Chapter B) --- Methods and materials / Chapter (I) --- Preparation of plating bacterial-Y1090 / Chapter (II) --- Plating the bacteriophage with blue-white visual selection / Chapter (III) --- Amplification of bacteriophage cDNA clones by PCR / Chapter (IV) --- Purification and quantitation of PCR products / Chapter (V) --- Cycle DNA sequencing of PCR products / Chapter (VI) --- Casting the sequencing gel / Chapter (VII) --- Sequencing by Pharmacia LKB A.L.F. DNA Sequencer / Chapter (VIII) --- Editing and saving the DNA sequence / Chapter (IX) --- Sending the DNA sequence to Genbank by E-mail / Chapter (X) --- Usage of the Genbank database / Chapter C) --- Results / Chapter D) --- Discussions / Chapter D.1 --- Application of human genomic project / Chapter D.2 --- Interpretation of the sequencing results / Chapter D.3 --- Quality of cDNA libraries and representation of mRNA population / Chapter D.4 --- "Gene expression profile in three different organs-heart, brain and liver" / Chapter D.5 --- Population study of the cDNA library / Chapter D.6 --- Isolation of a large number of novel genes by substraction cDNA library / Chapter D.7 --- Screening method to find out the complete coding sequence of interesting genes / Chapter D.8 --- Technical problems encountered and managed / Chapter PART 2: --- Identification of human cytosolic malate dehydrogenase by large scale human heart cDNA library sequencing / Chapter CHAPTER 1: --- Introduction of malate dehydrogenase / Chapter 1.1 --- Malate dehydrogenase--Kreb's cycle enzyme / Chapter 1.2 --- Two stereospecific forms of dehydrogenase / Chapter 1.3 --- NAD-binding domain / Chapter 1.4 --- The active site / Chapter 1.5 --- Comparison of surface properties between cMDH and mMDH / Chapter 1.6 --- N-terminal region and mitochondrial import / Chapter 1.7 --- Subunit-subunit interactions / Chapter 1.8 --- Physiological importance of malate dehydrogenase / Chapter 1.9 --- Secondary structure-total 11 β-strands and 9 α-helixes / Chapter 1.10 --- Objectives of the thesis / Chapter CHAPTER 2: --- Cloning and sequence analysis of human cytosolic malate dehydrogenase (hcMDH) / Chapter 2.1 --- Cloning of human cytosolic malate dehydrogenase (hcMDH) / Chapter 2.1.1 --- Methods and materials / Chapter 2.1.1.1 --- Cloning full length of hcMDH into expression vector pAED4 / Chapter 2.1.1.2 --- Preparation of competent cell-JM109 for transformation / Chapter 2.1.1.3 --- Minipreparation of plasmid DNA / Chapter 2.1.1.4 --- Midi-preparation of bacteriophage λDNA by QIAGEN´ёØ / Chapter 2.1.1.5 --- Titration of bacteriophage λ of human adult heart cDNA library / Chapter 2.1.1.6 --- Preparation of soft-agarose lysates / Chapter 2.1.1.7 --- Elution of DNA from agarose gel by GENECLEAN´ёØ / Chapter 2.1.2 --- Results / Chapter 2.1.3 --- Discussions / Chapter 2.2 --- Sequence analysis of human cytosolic malate dehydrogenase (hcMDH) / Chapter 2.2.1 --- Methods and materials: Autoread sequencing / Chapter (I) --- Annealing of primer to double-stranded template / Chapter (II) --- Sequencing / Chapter 2.2.2 --- Results and discussions / Chapter 2.3 --- Amino acids and protein structure analysis of cMDH / Chapter CHAPTER 3 : --- "Protein expression, partial purification and folding experiments of human cytosolic malate dehydrogenase (hcMDH)" / Chapter 3.1 --- Protein expression of hcMDH in E. coli / Chapter 3.1.1 --- Methods and materials / Chapter 3.1.1.1 --- Protein expression induced by IPTG / Chapter 3.1.1.2 --- Isoelectric focusing (IEF)-two dimensional gel electrophoresis / Chapter (I) --- First dimensional electrofocusing / Chapter (II) --- The second dimension SDS-PAGE electrophoresis / Chapter (III) --- Sample preparation / Chapter 3.1.2 --- Results / Chapter 3.1.3 --- Discussions / Chapter 3.1.3.1 --- The properties of expressed protein of hcMDH / Chapter 3.1.3.2 --- T7 expression system / Chapter 3.1.3.3 --- Strong φ 10 promoter / Chapter 3.1.3.4 --- E.coli BL21 host cell / Chapter 3.2 --- Partial purification and folding experiments of hcMDH / Chapter 3.2.1 --- Methods and materials / Chapter 3.2.1.1 --- Partial purification of hcMDH expressed protein / Chapter (I) --- Preparation of supernatant from E.coli crude extract / Chapter (II) --- Ion-exchange column chromatography / Chapter (III) --- Affinity chromatography / Chapter (IV) --- Gel filtration on a Sepharose CL-6B column / Chapter 3.2.1.2 --- Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) / Chapter 3.2.1.3 --- Staining the protein gel by the Coomassie Blue R-250 method / Chapter 3.2.1.4 --- Staining the protein gel by the Silver staining Method / Chapter 3.2.1.5 --- Quantitation of protein by the Bradford Method / Chapter 3.2.1.6 --- Native gel electrophoresis / Chapter 3.2.1.7 --- Malate dehydrogenase MDH enzyme staining method / Chapter 3.2.1.8 --- Malate dehydrogenase MDH enzyme assay / Chapter 3.2.1.9 --- Fast protein liquid chromatography (FPLC) / Chapter 3.2.1.10 --- Protein folding experiment / Chapter 3.2.1.11 --- Eukaryotic expression of hcMDH / Chapter 3.2.2 --- Results / Chapter 3.2.2.1 --- Partial purification by chromatography / Chapter 3.2.2.2 --- Native gel / Chapter 3.2.2.3 --- FPLC / Chapter 3.2.2.4 --- To aid folding of protein by adding NADH / Chapter 3.2.2.5 --- Eukaryotic expression / Chapter 3.2.3 --- Discussions / Chapter 3.2.3.1 --- Purification of malate dehydrogenase MDH / Chapter 3.2.3.2 --- "Methods for visualizing dehydrogenase enzymes, e.g. malate dehydrogenase" / Chapter 3.2.3.3 --- The presence of unfold hcMDH protein in bacteria / Chapter 3.2.3.4 --- Folding of protein by heat shock protein GroE / Chapter 3.2.3.5 --- Eukaryotic expression / Chapter CHAPTER 4: --- Master screening of single base change by PCR-SSCP (Single Strand Conformational Polymorphism) / Chapter 4.1 --- Theory of SSCP / Chapter 4.2 --- Methods and materials / Chapter 4.3 --- Results / Chapter 4.4 --- Discussions / Chapter 4.4.1 --- The procedure of SSCP / Chapter 4.4.2 --- An alternative quick detection method for polymorphism of hcMDH at position 565--by automatic sequencing / Chapter 4.4.3 --- Other detection methods-- RNA-PCR and ddF / Chapter 4.4.4 --- Parameters affecting sensitivity of SSCP / Chapter 4.4.5 --- Application of SSCP / Chapter CHAPTER 5: --- Southern hybridization and In situ hybridization / Chapter 5.1 --- Southern blot analysis of human cytosolic malate dehydrogenase (hcMDH) / Chapter 5.1.1 --- Methods and materials / Chapter (I) --- Transfer genomic DNA to Nylon membrane / Chapter (II) --- Synthesis of radiolabelling cDNA probe / Chapter (III) --- Pre-hybridization and hybridization reaction / Chapter 5.1.2 --- Results / Chapter 5.1.3 --- Discussions / Chapter 5.2 --- In situ hybridization / Chapter 5.2.1 --- Methods and materials / Chapter (I) --- Preparation of Dig labelling probe by random primed labelling / Chapter (II) --- Estimating the yield of Dig-labelled nucleic acids / Chapter (III) --- Denaturation and hybridization of the hcMDH probe with animal tissues / Chapter (IV) --- Color development of the tissue / Chapter 5.2.2 --- Results / Chapter 5.2.3 --- Discussions / Chapter 5.2.3.1 --- Cellular distribution of hcMDH / Chapter 5.2.3.2 --- The principle of in situ hybridization / Chapter 5.2.3.3 --- Specimen preparation / Chapter 5.2.3.4 --- Hybridization conditions / Chapter 5.2.3.5 --- "Ontogeny of MDH in rabbit fetal brain, heart and lung" / Appendixes: / "Appendix I: 531 random cDNA clones from clone no. J950 to K951 in human heart cDNA library sequencing project. The name of clones, accession number, the length of the partial sequence and percentage of match are listed" / Appendix II: The new accession no. of Novel clones in Genbank / "Appendix III: The enzymatic reaction, molecular weigth, specific activity and Michaelis constants of different sources of malate dehydrogenase" / Appendix IV: The full sequence of nucleic acids and amino acids of human cytosolic malate dehydrogenase hcMDH. Accession no. of hcMDH is U20352 in Genbank / Appendix V: Nucleotide sequences of the mouse cMDH gene / Appendix VI: Nucleotide sequences of the mouse mMDH gene / Appendix VII: Structural organization of the mouse cytosolic malate dehydrogenase and its comparison with that of the mouse mitochondrial malate dehydrogenase gene

by Sharon, Chui-Wah Luk. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (p. 128-146). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web.

by Nathan, Yiu-hung Yam. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 139-157). / Acknowledgements --- p.i / Table of Contents --- p.ii / Abstract --- p.viii / List of Abbreviations --- p.x / List of Tables --- p.xii / List of Figures --- p.xiii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- General introduction --- p.1 / Chapter 1.2 --- Aims of the present study --- p.2 / Chapter 1.3 --- Sequencing of an adult human heart cDNA library --- p.3 / Chapter 1.4 --- Rat/mouse CRIP --- p.5 / Chapter 1.5 --- LIM proteins --- p.13 / Chapter 1.6 --- Zinc-binding proteins --- p.17 / Chapter 1.7 --- Bacterial expression system using the pAED4 vector --- p.24 / Chapter Chapter 2 --- Identification and sequence analysis ofhCRHP --- p.26 / Chapter 2.1 --- Introduction --- p.26 / Chapter 2.2 --- Materials and methods --- p.29 / Chapter 2.2.1 --- Bacterial strains and vectors --- p.29 / Chapter 2.2.2 --- "Mediums, buffers and solutions" --- p.31 / Chapter 2.2.3 --- Bacteriophage clones preparation --- p.34 / Chapter 2.2.4 --- Amplification of clones by PCR --- p.35 / Chapter 2.2.5 --- Cycle sequencing of PCR products --- p.36 / Chapter 2.2.6 --- DNA sequences analysis --- p.38 / Chapter 2.3 --- Results --- p.39 / Chapter 2.3.1 --- Sequence analysis of hCRHP --- p.39 / Chapter 2.3.2 --- Comparison of hCRHP with CRIP --- p.52 / Chapter 2.3.3 --- Comparison of hCRHP with some LIM proteins --- p.56 / Chapter 2.4 --- Discussions --- p.61 / Chapter Chapter 3 --- Study of hCRHP at the nucleic acid level --- p.65 / Chapter 3.1 --- Introduction --- p.65 / Chapter 3.2 --- Materials and methods --- p.66 / Chapter 3.2.1 --- Animals --- p.66 / Chapter 3.2.2 --- "Mediums, buffers, enzymes and solutions" --- p.66 / Chapter 3.2.3 --- Preparation of total RNA --- p.70 / Chapter 3.2.3.1 --- Preparation of RNA by the CsCl method --- p.70 / Chapter 3.2.3.2 --- Preparation of RNA by the AGPC method --- p.71 / Chapter 3.2.4 --- Northern hybridization of hCRHP --- p.72 / Chapter 3.2.4.1 --- Formaldehyde agarose gel electrophoresis --- p.72 / Chapter 3.2.4.2 --- Preparation of radioactive probe --- p.73 / Chapter 3.2.4.3 --- RNA transfer and Northern hybridization --- p.74 / Chapter 3.2.5 --- Preparation of human genomic DNA --- p.77 / Chapter 3.2.6 --- Southern hybridization of hCRHP --- p.78 / Chapter 3.2.6.1 --- Restriction cutting and agarose gel electrophoresis of genomic DNA --- p.78 / Chapter 3.2.6.2 --- DNA transfer and Southern hybridization --- p.79 / Chapter 3.3 --- Results --- p.80 / Chapter 3.3.1 --- Southern hybridization of hCRHP --- p.80 / Chapter 3.3.2 --- Identification of hCRHP in neonatal human heart --- p.83 / Chapter 3.3.3 --- Tissue distribution of CRIP mRNA in rat tissues --- p.85 / Chapter 3.3.4 --- Time course of CRIP expression in rat heart --- p.85 / Chapter 3.4 --- Discussions --- p.87 / Chapter Chapter 4 --- Subcloning and expression of hCRHP --- p.89 / Chapter 4.1 --- Introduction --- p.89 / Chapter 4.2 --- Materials and methods --- p.90 / Chapter 4.2.1 --- Bacterial strains and vectors --- p.90 / Chapter 4.2.2 --- "Mediums, buffers, enzymes and solutions" --- p.92 / Chapter 4.2.3 --- Subcloning of hCRHP into pAED4 --- p.98 / Chapter 4.2.3.1 --- Primers design and PCR --- p.98 / Chapter 4.2.3.2 --- Purification of PCR products by Geneclean II´ёØ (BIO 101 Inc) --- p.99 / Chapter 4.2.3.3 --- Restriction digestion of purified PCR product and pAED4 --- p.100 / Chapter 4.2.3.4 --- Ligation and transformation of hCRHP --- p.101 / Chapter 4.2.3.5 --- Amplification and purification of pAED4-hCRHP --- p.103 / Chapter 4.2.4 --- Expression of hCRHP --- p.105 / Chapter 4.2.4.1 --- Induction of hCRHP expression --- p.105 / Chapter 4.2.4.2 --- SDS-PAGE and protein detection --- p.106 / Chapter 4.3 --- Results --- p.108 / Chapter 4.3.1 --- Subcloning of hCRHP into pAED4 --- p.108 / Chapter 4.3.2 --- Induction and optimization of hCRHP expression --- p.110 / Chapter 4.4 --- Discussions --- p.117 / Chapter Chapter 5 --- Partial purification and isoelectric focusing of hCRHP --- p.120 / Chapter 5.1 --- Introduction --- p.120 / Chapter 5.2 --- Materials and methods --- p.121 / Chapter 5.2.1 --- "Mediums, buffers and mediums" --- p.121 / Chapter 5.2.2 --- Purification of hCRHP by ammonium sulphate precipitation --- p.121 / Chapter 5.2.3 --- Purification of hCRHP by hydrochloric acid extraction --- p.122 / Chapter 5.2.4 --- Purification of hCRHP by ultrafiltration --- p.123 / Chapter 5.2.5 --- Isoelectric focusing of hCRHP --- p.127 / Chapter 5.3 --- Results --- p.128 / Chapter 5.3.1 --- Partial purification of hCRHP by ammonium sulphate precipitation --- p.128 / Chapter 5.3.2 --- Partial purification of hCRHP by hydrochloric acid extraction --- p.128 / Chapter 5.3.3 --- Partial purification of hCRHP by ultrafiltration --- p.131 / Chapter 5.3.4 --- Isoelectric focusing of hCRHP --- p.133 / Chapter 5.4 --- Discussions --- p.133 / Chapter Chapter 6 --- Discussions --- p.136 / Chapter 6.1 --- The possible role(s) of hCRHP/CRIP --- p.136 / Chapter 6.2 --- Future prospects --- p.137 / References --- p.139 / Appendix 1 --- p.158

by Lam Wai Yip. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 184-195). / Contents --- p.I - IV / Abstract --- p.V / Abbreviations --- p.VI / List of Tables and Figures --- p.VII - XV / Chapter Chapter One: --- Introduction / Part I / Chapter 1.1 --- Human genome project --- p.1 / Chapter 1.2 --- Progress of human genome project --- p.2 / Chapter 1.3 --- Human heart cDNA sequencing --- p.3 / Chapter 1.4 --- Significance of the human heart cDNA library project --- p.5 / Chapter 1.5 --- Homology search tools for cDNA sequences alignment --- p.5 / Part II / Chapter 1.6 --- Investigation of a human heart cDNA clone A076 --- p.7 / Chapter 1.7 --- General introduction of Heat Shock Proteins (HSPs) --- p.7 / Chapter 1.7.1 --- Definition of HSP --- p.8 / Chapter 1.7.2 --- Discovery of HSP --- p.10 / Chapter 1.7.3 --- Transcriptional regulation of heat shock genes --- p.11 / Chapter 1.7.4 --- Nomenclature of HSPs --- p.13 / Chapter 1.7.5 --- HSP110 --- p.13 / Chapter 1.7.6 --- HSP90 --- p.14 / Chapter 1.7.7 --- HSP70 --- p.15 / Chapter 1.7.8 --- HSP60 --- p.17 / Chapter 1.7.9 --- Ubiquitin - HSP8 --- p.19 / Chapter 1.7.10 --- HSP27 --- p.20 / Chapter 1.8 --- The theme of this thesis --- p.28 / Chapter Chapter Two: --- Method and Materials / Chapter 2.1 --- The human heart cDNA library --- p.29 / Chapter 2.2 --- Plating out the cDNA library --- p.29 / Chapter 2.3 --- DNA amplification --- p.31 / Chapter 2.4 --- DNA sequencing reaction - Cycle sequencing reaction --- p.32 / Chapter 2.5 --- Operation of the A.L.F. DNA sequencer --- p.33 / Chapter 2.5.1 --- Preparation of the gel cassette --- p.33 / Chapter 2.5.2 --- Preparation of the acrylamide gel --- p.34 / Chapter 2.5.3 --- Fitting the gel cassette into the electrophoresis unit --- p.35 / Chapter 2.5.4 --- Settings of electrophoresis --- p.36 / Chapter 2.6 --- Comparison of DNA sequences to databases --- p.37 / Chapter 2.7 --- Programming for sending cDNA sequences to NCBI --- p.38 / Chapter 2.8 --- Storage of sequence data --- p.39 / Chapter 2.9 --- Synthesis and purification of primers --- p.40 / Chapter 2.10 --- Connection of cDNA clones using Polymerase Chain Reaction (PCR) --- p.41 / Chapter 2.11 --- Purification of DNA fragment from agarose gels by GENECLEAN´ёØ --- p.42 / Chapter 2.12 --- "Preparation of competent Escherichia coli for transformation (Hanahan, 1986)" --- p.43 / Chapter 2.13 --- Transformation of Plasmid into Competent Escherichia coli --- p.44 / Chapter 2.14 --- "Small scale preparation of plasmid DNA (Sambrook et al.,1989" --- p.45 / Chapter 2.15 --- Large scale plasmid preparation by QIAGEN´ёØ --- p.46 / Chapter 2.16 --- DNA sequencing reaction - Unicycle sequencing reaction --- p.48 / Chapter 2.17 --- Synthesis of Radiolabeled DNA probe --- p.49 / Chapter 2.18 --- "Isolation of genomic DNA from human blood cells (Thomas A. Ciulla, 1988)" --- p.51 / Chapter 2.19 --- Southern blotting --- p.52 / Chapter 2.20 --- Prehybridization and hybridization procedure for Southern blot analysis --- p.54 / Chapter 2.21 --- "AGPC-RNA extraction method (Chomczynski and Sacchi 1987, modifed)" --- p.56 / Chapter 2.22 --- Electrophoresis of RNA through gels containing formaldehyde --- p.58 / Chapter 2.23 --- First-Strand cDNA synthesis --- p.59 / Chapter 2.24 --- Use of T7 RNA polymerase to direct expression of the cloned hsp27b gene (A076&B490) --- p.60 / Chapter 2.25 --- "Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (Laemmli, 1970)" --- p.61 / Chapter 2.26 --- Staining of the Gel by the Commassie Blue Method --- p.63 / Chapter Chapter Three: --- Results / Part I / Chapter 3.1 --- Sample results of Sequencing a few clones --- p.64 / Chapter 3.2 --- A Catalogue of 497 cDNA clones obtained from human heart cDNA sequencing --- p.71 / Chapter 3.3 --- Submission of novel sequences to genbank --- p.81 / Chapter 3.4 --- A Catalogues of genes that are expressed in the adult human heart --- p.83 / Chapter 3.5 --- The use of the programmes to assist the sending and receiving of sequence data E-mail message --- p.90 / Chapter 3.5.1 --- The use of the SENDMAIL.EXE programme --- p.91 / Chapter 3.5.2 --- "The use of the EDITBLN.EXE, ALLFILE.EXE and DATABASE.EXE" --- p.95 / Part II / Chapter 3.6 --- DNA sequence profiles of cDNA clones A076 and B490 --- p.105 / Chapter 3.7 --- Ligation of cDNA clones using Polymerase Chain Reaction (PCR) --- p.112 / Chapter 3.8 --- Cloning of the PCR product A076&B490 into the pAED4 expression vector --- p.117 / Chapter 3.9 --- Unicycle sequencing of the subcloned insert A076&B490 --- p.121 / Chapter 3.10 --- Southern hybridization of hsp27b (A076&B490) --- p.125 / Chapter 3.11 --- Results of RT-PCR and PCR --- p.127 / Chapter 3.12 --- Expression pAED4-A076&B490 in E.coli --- p.133 / Chapter Chapter Four: --- Discussion / Part I / Chapter 4.1 --- EST characterization --- p.138 / Chapter 4.2 --- Further investigation --- p.140 / Chapter 4.3 --- Disadvantage of randomly picked cDNA sequencing --- p.141 / Chapter 4.4 --- Problem of GenBank database searching --- p.141 / Part II / Chapter 4.5 --- The DNA sequence of A076 and B490 --- p.143 / Chapter 4.6 --- Ligation of HSP27B by using PCR --- p.144 / Chapter 4.7 --- Analysis of the DNA and protein sequence ofhsp27b (A076&B490) --- p.145 / Chapter 4.8 --- Southern hybridization of human hsp27b --- p.153 / Chapter 4.9 --- "RT-PCR and PCR of first strand cDNA with primers A076-ATG, A076-mid and oligo dT" --- p.153 / Chapter 4.10 --- Expression of human hsp27b --- p.154 / Chapter 4.11 --- The possible roles of human hsp27b --- p.156 / Chapter 4.12 --- Further analysis --- p.160 / Appendix I --- p.161-182 / Appendix II --- p.183 / References --- p.184-195

Ng Dean Yew, Dennis. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 85-98). / Abstract --- p.i / Acknowledgments --- p.iii / Table of contents --- p.iv / List of figures --- p.vii / List of tables --- p.ix / List of abbreviation --- p.x / Chapter Chapter 1 --- Introduction / Chapter 1.1. --- History --- p.1 / Chapter 1.2. --- Classification and structure of Epstein-Barr Virus --- p.2 / Chapter 1.3. --- Genomic organization of EBV --- p.3 / Chapter 1.4. --- Replication cycle of EBV --- p.5 / Chapter 1.5. --- EBV latent and lytic cycle proteins --- p.6 / Chapter 1.6. --- Clinical diseases associated with EBV Infection --- p.11 / Chapter 1.7. --- Association of EBV and NPC --- p.13 / Chapter 1.8. --- EBV serological markers in the diagnosis of NPC --- p.13 / Chapter 1.9. --- Sources of EBV-specific DNase --- p.15 / Chapter 1.10. --- Characteristics of Epstein-Barr virus alkaline DNase --- p.15 / Chapter 1.11. --- Aim of the project --- p.18 / Chapter Chapter 2 --- Materials & Methods / Chapter 2.1. --- Molecular cloning --- p.19 / Chapter 2.1.1. --- Cell culture --- p.19 / Chapter 2.1.2. --- mRNA purification --- p.19 / Chapter 2.1.3. --- First strand cDNA synthesis --- p.21 / Chapter 2.1.4. --- Polymerase chain reaction (PCR) of cDNA --- p.21 / Chapter 2.1.5. --- Purification of PCR product after gel electrophoresis --- p.22 / Chapter 2.1.6. --- Ligation of PCR amplified DNase gene into pUC18 Sma/BAP vector --- p.23 / Chapter 2.1.7. --- Transformation by electroporation --- p.24 / Chapter 2.1.7.1. --- Cell preparation --- p.24 / Chapter 2.1.7.2. --- Electroporation procedure --- p.25 / Chapter 2.2. --- Extraction ofplasmid DNA --- p.28 / Chapter 2.2.1. --- Boiling preparation --- p.28 / Chapter 2.2.2. --- Plasmid digestion --- p.29 / Chapter 2.3. --- Large-scale purification ofplasmid --- p.29 / Chapter 2.4. --- Small-scale purification ofplasmid --- p.32 / Chapter 2.5. --- DNA sequencing --- p.33 / Chapter 2.5.1. --- Annealing of primer to template DNA --- p.33 / Chapter 2.5.2. --- Labelling reaction --- p.34 / Chapter 2.5.3. --- Sequencing termination reaction --- p.35 / Chapter 2.5.4. --- Prepartion of sequencing gel --- p.36 / Chapter 2.5.5. --- Autoradiography of sequencing gel --- p.38 / Chapter 2.6. --- Epitope mapping --- p.39 / Chapter 2.6.1. --- Processing of EBV- specific DNase peptides --- p.39 / Chapter Chapter 3 --- Results / Chapter 3.1. --- Molecular cloning --- p.41 / Chapter 3.1.1. --- Cell culture --- p.41 / Chapter 3.1.2. --- mRNA purification --- p.42 / Chapter 3.1.3. --- PCR amplification --- p.42 / Chapter 3. 1.4 --- DNA purification of PCR product --- p.42 / Chapter 3.1.5. --- Molecular cloning of PCR amplified DNase gene into pUC18 SmaI/BAP vector --- p.44 / Chapter 3.1.6. --- Transformation by electroporation --- p.46 / Chapter 3.1.7. --- Extraction of plasmid DNA --- p.48 / Chapter 3.1.7.1. --- Boiling preparation --- p.48 / Chapter 3.1.8. --- Plasmid digestion --- p.51 / Chapter 3.2. --- DNA sequencing --- p.51 / Chapter 3.2.1. --- Comparison of B95-8 EBV-speicific DNase gene with gene sequence of EBV in GeneBank --- p.50 / Chapter 3.2.2. --- Comparison of 5' end of Raji & B95-8 EBV derived EBV-specific DNase gene --- p.57 / Chapter 3.2.3. --- Comparison of the 3'end of the Raji and B95-8 denved EBV-specific DNase gene --- p.63 / Chapter 3.2.4. --- Amino acid sequence homology between B95-8 & Raji EBV-specific DNase --- p.64 / Chapter 3.2.5. --- Amino acid sequence comparison between the 3' end of the B95-8 EBV DNase protein with that of the Raji EBV DNase protein --- p.62 / Chapter 3.3. --- Epitope mapping --- p.67 / Chapter 3.3.1. --- Amino acid key --- p.67 / Chapter 3.3.2. --- Amino acid sequence of peptides --- p.73 / Chapter 3.3.2. --- O.D. readings at 492nm of five histologically proven NPC sera --- p.74 / Chapter Chapter 4 --- Discussions / Chapter 4.1. --- Overall strategy --- p.75 / Chapter 4 2 --- Significance of EBV-specific DNase as marker for NPC --- p.76 / Chapter 4.3. --- Characterization of EBV-specific DNase --- p.76 / Chapter 4.4. --- Molecular cloning of PCR amplified gene into PUC18 SmaI/BAP vector --- p.77 / Chapter 4.4.1. --- Cell culture --- p.77 / Chapter 4.4.2. --- PCR amplification --- p.73 / Chapter 4.4.3. --- "Blunting,kinasing and ligation of EBV-specific DNase cDNA into pUC18 vector" --- p.78 / Chapter 4.4 .4 --- .Transformation by electroporation --- p.80 / Chapter 4.4.5. --- Restriction enzyme digestion of pUC18/EBV-DNase plasmid … --- p.81 / Chapter 4.5. --- DNA sequencing --- p.81 / Chapter 4.6. --- Epitope mapping --- p.83 / Reference --- p.85

by Kar-Keung Ching. / "May 2000." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (p. 154-173). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

by Patrick, Tik-wan Law. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 144-155). / Acknowledgements --- p.i / Contents --- p.ii / Abstract --- p.vi / Abbreviations --- p.viii / List of figures --- p.ix / List of tables --- p.xiv / Chapter Chapter One: --- Introduction --- p.1-17 / Chapter 1.1 --- General introduction --- p.1 / Chapter 1.2 --- The Human genome project --- p.2 / Chapter 1.3 --- The EST approach --- p.3 / Chapter 1.4 --- Significance of the EST approach --- p.3 / Chapter 1.5 --- Human heart cDNA sequencing --- p.5 / Chapter 1.6 --- Significance of the human adult heart EST project --- p.7 / Chapter 1.7 --- Ribosomal proteins --- p.8 / Chapter 1.7.1 --- The ribosomal constituents --- p.8 / Chapter 1.7.2 --- Eukaiyotic ribosomal proteins --- p.10 / Chapter 1.8 --- Mammalian ribosomal proteins --- p.11 / Chapter 1.8.1 --- Evolution of mammalian ribosomal proteins --- p.11 / Chapter 1.8.2 --- Significance of mammalian ribosomal proteins --- p.12 / Chapter 1.9 --- Possible functional roles of ribosomal protein --- p.14 / Chapter 1.10 --- Nomenclature of ribosomal proteins --- p.16 / Chapter 1.11 --- The theme of the thesis --- p.17 / Chapter Chapter Two: --- Materials and Methods --- p.18-49 / Chapter 2.1 --- Cycle sequencing --- p.18 / Chapter 2.1.1 --- Plating out the cDNA library --- p.18 / Chapter 2.1.2 --- Amplification of the cDNA clones by PCR --- p.19 / Chapter 2.1.3 --- Purification and quantitation of the PCR product --- p.20 / Chapter 2.1.4 --- Cycle DNA sequencing --- p.20 / Chapter 2.2 --- Cloning of hrpL29 in pUC 18 cloning vector --- p.21 / Chapter 2.2.1 --- Amplification of the phage by plate lysate --- p.21 / Chapter 2.2.2 --- Amplification of the insert by PCR --- p.22 / Chapter 2.3 --- Screening for hrpL29 transformant --- p.22 / Chapter 2.3.1 --- Mini-preparation of plasmid DNA (Sambrook et al，1989) --- p.22 / Chapter 2.3.2 --- Large scale preparation of plasmid DNA --- p.24 / Chapter 2.4 --- Primer design for cloning of an intron of hrpL29 --- p.26 / Chapter 2.5 --- Isolation of the intron of hrpL29 by PCR --- p.26 / Chapter 2.6 --- Restricted endonuclease digestion --- p.27 / Chapter 2.7 --- Purification of DNA from the agarose gel --- p.27 / Chapter 2.8 --- Dephosphorylation of linearized plasmid DNA --- p.29 / Chapter 2.9 --- DNA ligation --- p.29 / Chapter 2.10 --- "Preparation of competent bacterial cells for transformation (Hanahan,1985)" --- p.30 / Chapter 2.11 --- Plasmid DNA Transformation --- p.31 / Chapter 2.12 --- Unicycle DNA sequencing by T7 polymerase (Pharmacia) --- p.32 / Chapter 2.13 --- Synthesis of radiolabelled DNA probe --- p.33 / Chapter 2.14 --- "Oligonucleotide synthesis, deprotection and purification" --- p.34 / Chapter 2.14.1 --- Oligonucleotide synthesis --- p.34 / Chapter 2.14.2 --- Deprotection and purification of oligonucleotides --- p.35 / Chapter 2.15 --- Southern analysis --- p.36 / Chapter 2.15.1 --- "Isolation of genomic DNA from leukocytes (Ciulla et al,1988)" --- p.36 / Chapter 2.15.2 --- Restricted digestion and fractionation of genomic DNA --- p.37 / Chapter 2.15.3 --- Southern transfer of DNA onto a membrane support --- p.37 / Chapter 2.15.4 --- Prehybridization of the Southern blot --- p.40 / Chapter 2.15.5 --- Hybridization of the Southern blot --- p.40 / Chapter 2.16 --- Northern analysis --- p.41 / Chapter 2.16.1 --- "Isolation of total RNA by using the AGPC-RNA method (Chomczynski and Sacchi,1987, modified)" --- p.41 / Chapter 2.16.2 --- Separation of total RNA by electrophoresis and transfer onto a membrane support --- p.43 / Chapter 2.16.3 --- Prehybridization of the Northern blot --- p.46 / Chapter 2.16.4 --- Hybridization of the Northern blot --- p.47 / Chapter 2.17 --- First strand cDNA synthesis (Pharmacia) --- p.48 / Chapter 2.18 --- PCR of the first strand cDNA --- p.48 / Chapter Chapter Three: --- Results --- p.50-113 / Chapter 3.1 --- Partial sequencing of adult human heart cDNA clones --- p.50 / Chapter 3.2 --- DNA homology searching by using the program BLASTN --- p.52 / Chapter 3.2.1 --- Catalogue of the 502 ESTs of the cardiovascular system --- p.54 / Chapter 3.2.2 --- Classification and frequency of the human adult heart cDNA clones --- p.63 / Chapter 3.3 --- Submission of the cDNA sequences to NCBI --- p.64 / Chapter 3.4 --- Pattern of gene expression in the human adult cardiovascular system --- p.66 / Chapter 3.5 --- "Sequence determination of hrpL29 (Law et. al., 1996)" --- p.72 / Chapter 3.5.1 --- Cycle Taq sequencing of hrpL29 --- p.72 / Chapter 3.5.2 --- Subcloning of the hrpL29 cDNA insert into the pUC18 DNA cloning vector --- p.75 / Chapter 3.5.3 --- Unicycle T7 sequencing of hrpL29 --- p.77 / Chapter 3.6 --- Sequence alignment and comparison of hrpL29 with other known sequences in the databases --- p.79 / Chapter 3.7 --- The primary structure of hrpL29 --- p.83 / Chapter 3.8 --- Results of RT-PCR and PCR --- p.88 / Chapter 3.9 --- Genomic analysis of hrpL29 --- p.92 / Chapter 3.9.1 --- Isolation of the first intron of hrpL29 --- p.92 / Chapter 3.9.2 --- Southern analysis of hrpL29 --- p.97 / Chapter 3.10 --- Northern analysis of hrpL29 --- p.103 / Chapter 3.10.1 --- Tissue distribution of hrpL29 mRNA in rat tissues --- p.103 / Chapter 3.10.2 --- Time course of hRPL29 expression in mouse heart --- p.106 / Chapter 3.10.3 --- Time course of hRPL29 expression in mouse brain --- p.110 / Chapter Chapter Four: --- Discussion --- p.114-139 / Chapter 4.1 --- Characterization of the ESTs --- p.114 / Chapter 4.2 --- Significance of the heart EST project --- p.116 / Chapter 4.3 --- Redundancy of the EST sequencing --- p.118 / Chapter 4.4 --- The importance of frequent database searching --- p.119 / Chapter 4.5 --- The importance of an efficient comparison algorithm --- p.120 / Chapter 4.6 --- Human ribosomal protein L29 (hRPL29) --- p.122 / Chapter 4.7 --- Internal duplication in hRPL29 --- p.124 / Chapter 4.8 --- Primary structure analysis of hRPL29 --- p.126 / Chapter 4.9 --- RT-PCR and PCR of the first strand cDNA with primers using the C095-ATG and dT primer --- p.128 / Chapter 4.10 --- Southern analysis of hrpL29 --- p.128 / Chapter 4.11 --- Northern analysis of hrpL29 --- p.133 / Chapter 4.11.1 --- Tissue distribution of the mRNA species of hrpL29 --- p.133 / Chapter 4.11.2 --- Time course of hRPL29 expression in mouse heart and brain --- p.134 / Chapter 4.12 --- Possible functional role of hRPL29 --- p.135 / Chapter 4.13 --- Further aspects --- p.137 / Appendix --- p.140-143 / References --- p.144-155