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The application of hemagglutination tests to turnip mosaic virus.Raptis, Leda Helen January 1975 (has links)
No description available.
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The application of hemagglutination tests to turnip mosaic virus.Raptis, Leda Helen January 1975 (has links)
No description available.
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Purification and analysis of autoimmune antibody reactive with single stranded DNAUnknown Date (has links)
This study evaluated two methods for the isolation and purification of anti-DNA antibodies. A two-step affinity purification with streptavidin (SA) biotinylated oligodeoxythymidine (dT) M-280 and protein G Dynabeadsª was compared to a two step method using Melon(TM) Gel and cellulose DNA. Although Melon gel allowed for faster antibody purification and a higher recovery rate it gave a product of less purity than the magnetic bead method. Further characterization of the antibodies was done by PhastGel(TM) non-reducing SDS-PAGE and isoelectric focusing in order to analyze purity and confirm the polyclonal nature of anti-DNA antibodies. Agilent 2100, with a higher resolution then SDS-PAGE, revealed possible subclasses of different MW not detected by SDS-PAGE. ELISA showed that all four IgG antibody subclasses were present, while Western blot confirmed the presence of human IgGs. Ultraviolet spectroscopy, Agilent, and fluorescence based assays were used to demonstrate DNA hydrolytic activity of purified anti-DNA antibody. / by Anna M. Kats. / Thesis (M.S.)--Florida Atlantic University, 2008. / Includes bibliography. / Electronic reproduction. Boca Raton, FL : 2008 Mode of access: World Wide Web.
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Monitoring potato leafroll virus movement in differentially aged potato (Solanum tubersom L.) plants with an immunosorbent direct tissue blotting assayWhitworth, Jonathan L. 26 April 1993 (has links)
Potato leafroll virus (PLRV) causes yield and quality losses in
potato. PLRV is identified by plant symptoms and serological tests
such as an enzyme-linked immunosorbent assay (ELISA). A similar
serological test, direct tissue blotting assay (DTBA), was used to
detect and monitor PLRV movement in field-inoculated Russet Burbank
plants and plant tissues from Russet Burbank and Russet Norkotah seed
tubers submitted by growers for winter certification tests.
DTBA was as accurate as ELISA and easier to use for detecting
tuber-perpetuated PLRV in stems and petioles of plants grown from
grower-submitted seed tubers. ELISA detected twice as many PLRV
positives as DTBA in leaflet tests. DTBA detected PLRV in tuber tissue
but results matched ELISA in only 74% or less of the samples. Results
of DTBA tuber tests were sometimes difficult to interpret while stem
and petiole results were distinct and unambiguous.
As inoculations were delayed later in the season and as plants
matured, PLRV infection levels decreased sharply, most often within a
two week period in early July. In same-age plants inoculated 43 days
after planting but 18 days apart, early inoculation produced higher
PLRV levels. Conversely, when same-age plants were inoculated 62 days
after planting but 19 days apart, late inoculation produced higher PLRV
levels. This discrepancy is not fully understood, but larger tuber
size at the later inoculation probably produced a stronger sink for
source-to-sink translocation of nutrients and phloem-limited viruses.
Results of DTBA winter grow-out tests of summer-infected tubers
approximated those of ELISA and visual inspections. Indirect DTBA
testing of tubers utilizing stem and petiole tissues from winter growout
plants detected more PLRV than directly testing tuber tissue 21
days post inoculation in summer. DTBA detected current season
(primary) PLRV less reliably than secondary (tuber-borne) PLRV, similar
to reported ELISA results.
PLRV infection increased tuber numbers but decreased size. Size
reduction was most evident in plants infected early in the season.
Average tuber size in healthy plots was always larger than the average
tuber size in infected plots. Within an infected plant, small tubers
tended to be infected less often than large tubers. / Graduation date: 1993
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