The purpose of this study was to evaluate the effects of same-day strength training on velocity and accuracy of a tennis serve among five female Division I intercollegiate athletes at the second and sixth week of a strength training program. Velocity and accuracy of 20 tennis serves were measured approximately four hours after a morning split-body (lower legs and trunk) strength training session termed the lift-day (LD) and were compared to measures taken on days that no lifting took place, termed the non-lift day (NLD). For each test day, velocity was multiplied by accuracy to provide an overall serve performance score for each NLD and LD. An ANOVA revealed that there was no significant difference in any of the measured variables between the NLD and the LD at any time period throughout the study. A mean serve performance score difference between NLD (77.56) and LD (78.05) of 0.49 was not statistically different [p = 0.84]. The results of this study suggest that female collegiate tennis players may strength-train with no significant effect on same-day serve performance following adequate recovery.