Este trabalho teve como objetivo estudar o comportamento espaço-temporal das variáveis limnológicas, no Ribeirão Bonito (SP) a fim de avaliar a descontinuidade ocasionada pelas fontes poluidoras e o processo de autodepuração. Para tanto, foram determinadas variáveis físico-químicas, biológicas, climatológicas e hidrológicas, no eixo longitudinal do rio e utilizado como ferramenta para diferentes cenários de simulações das concentrações de oxigênio dissolvido, o modelo QUAL2E. Foi possível observar, que muitas das variáveis limnológicas estudadas no Ribeirão Bonito sofrem \"descontinuidades\" devido à entrada de esgoto. As concentrações de oxigênio dissolvido têm um gradiente positivo da nascente até a estação 4, sofrendo uma descontinuidade após a estação 6 onde detecta-se o primeiro lançamento de esgoto. O comportamento dessa variável após a entrada de esgoto foi distinto nos dois períodos estudados. As análises de \"Cluster\" e PCA (Análise dos Componentes Principais), realizadas com as médias das variáveis limnológicas, distinguiram diferentes grupos no perfil longitudinal do ribeirão Bonito. Essa distinção pode ser atribuída às já citadas entradas de efluentes e também em decorrência da presença do tributário do Curtume. Pode-se constatar que mesmo com o auxílio natural do processo de autodepuração, muitas das variáveis estudadas não se enquadram aos padrões do corpo receptor conforme previsto pela resolução número 20/86 do CONAMA, sugerindo assim, neste trabalho, um grau de tratamento dos efluentes para que estes padrões sejam respeitados. / The objective of the present paper is to study the spatial-temporal behaviour of the limnological variables, in Bonito stream (SP), in order to evaluate the discontinuity occasioned by the sources of pollution and the self-cleaning process. To this effect, a survey was made to determine the physical-chemical, biological, climatological and hydrological variables in the longitudinal axis of the river and which utilized the QUAL2E model as a tool for simulating the concentrations of dissolved oxygen under different scenarios. It was possible to observe that many of the limnological variables studied in the Bonito rivulet suffer \"discontinuities\" due the entry of sewage. The concentrations of dissolved oxygen show a positive gradient from the source of the watercourse until station 4, and suffer a discontinuity after station 6, where the first discharge of sewage begins. The behaviour of this variable after the entry of sewage differed during the two periods studied. Furthermore, the \"Cluster\" and PCA (Principal Component Analyses) carried out upon the averages of the limnological variables distinguished different groups in the longitudinal profile of the Bonito rivulet. This distinction can be attributed to the above-mentioned entries of effluents and also resulting from the presence of the Curtume tributary. It can be verified that even with the natural assistance of the self-cleaning process, many of the variables studied fail to meet the standards of the receiving body, as stipulated by the CONAMA resolution number 20/86, in view of which, the present study suggests a degree of effluent treatment in order for these standards to be respected.

Sewage misconnections currently pose severe threats to water quality especially in urban areas in the UK. These misconnections lead to the discharge of untreated wastewater into receiving watercourses via surface water outfalls. Regular discharges from this source could lead to these watercourses failing to meet standards set by the Water Framework Directives. Despite the impacts resulting from this urban diffuse pollutant source, research into this area is limited. The study area is the River Lee, which is known to experience issues with water quality especially within the heavily urbanized lower Lee section. Misconnections are one of the major contributors to the poor water quality status of the River. In this study, the Lee was investigated using several parameters. The approach involved the monitoring of coliform bacteria to detect outfalls where misconnections were likely, then biofilm samples below selected outfalls were used for both clean and polluted sites to look for community types. Results showed severe pollution within some sections of the River particularly within the lower Lee. Pymmes Brook was used as a case study, both coliform bacteria and biofilm communities below outfalls were further investigated for clean and polluted sites. Results from the Pymmes Brook study also showed that this watercourse was experiencing severe issues with water quality, with elevated levels of coliform bacteria identified below polluted outfalls. Biofilm community data obtained during the pilot and experimental phases of study were analyzed using a range of multivariate techniques. Results of the analysis showed consistent x patterns in community structure within sites with similar water quality, with indicator species identified. Communities below polluted outfalls were composed mainly of species tolerant of organic pollution thus indicating a response of communities to misconnection discharges. Currently, the identification of misconnections are carried out through trackbacks, a process which is expensive and time consuming. During this study an inexpensive and rapid approach is proposed for assessing misconnections within a catchment. Using this method, misconnection hotspots are mapped out using coliform bacteria and biofilm communities. Using catchment data and predicted coliform bacteria from enumerated counts, misconnections within these hotspots are assessed. Having tested this approach on Pymmes Brook, a tributary of the River Lee, the results obtained were promising. Pending further verification, this system provides an economical and rapid tool for the assessment of misconnections within a catchment.

Este trabalho teve como objetivo estudar o comportamento espaço-temporal das variáveis limnológicas, no Ribeirão Bonito (SP) a fim de avaliar a descontinuidade ocasionada pelas fontes poluidoras e o processo de autodepuração. Para tanto, foram determinadas variáveis físico-químicas, biológicas, climatológicas e hidrológicas, no eixo longitudinal do rio e utilizado como ferramenta para diferentes cenários de simulações das concentrações de oxigênio dissolvido, o modelo QUAL2E. Foi possível observar, que muitas das variáveis limnológicas estudadas no Ribeirão Bonito sofrem \"descontinuidades\" devido à entrada de esgoto. As concentrações de oxigênio dissolvido têm um gradiente positivo da nascente até a estação 4, sofrendo uma descontinuidade após a estação 6 onde detecta-se o primeiro lançamento de esgoto. O comportamento dessa variável após a entrada de esgoto foi distinto nos dois períodos estudados. As análises de \"Cluster\" e PCA (Análise dos Componentes Principais), realizadas com as médias das variáveis limnológicas, distinguiram diferentes grupos no perfil longitudinal do ribeirão Bonito. Essa distinção pode ser atribuída às já citadas entradas de efluentes e também em decorrência da presença do tributário do Curtume. Pode-se constatar que mesmo com o auxílio natural do processo de autodepuração, muitas das variáveis estudadas não se enquadram aos padrões do corpo receptor conforme previsto pela resolução número 20/86 do CONAMA, sugerindo assim, neste trabalho, um grau de tratamento dos efluentes para que estes padrões sejam respeitados. / The objective of the present paper is to study the spatial-temporal behaviour of the limnological variables, in Bonito stream (SP), in order to evaluate the discontinuity occasioned by the sources of pollution and the self-cleaning process. To this effect, a survey was made to determine the physical-chemical, biological, climatological and hydrological variables in the longitudinal axis of the river and which utilized the QUAL2E model as a tool for simulating the concentrations of dissolved oxygen under different scenarios. It was possible to observe that many of the limnological variables studied in the Bonito rivulet suffer \"discontinuities\" due the entry of sewage. The concentrations of dissolved oxygen show a positive gradient from the source of the watercourse until station 4, and suffer a discontinuity after station 6, where the first discharge of sewage begins. The behaviour of this variable after the entry of sewage differed during the two periods studied. Furthermore, the \"Cluster\" and PCA (Principal Component Analyses) carried out upon the averages of the limnological variables distinguished different groups in the longitudinal profile of the Bonito rivulet. This distinction can be attributed to the above-mentioned entries of effluents and also resulting from the presence of the Curtume tributary. It can be verified that even with the natural assistance of the self-cleaning process, many of the variables studied fail to meet the standards of the receiving body, as stipulated by the CONAMA resolution number 20/86, in view of which, the present study suggests a degree of effluent treatment in order for these standards to be respected.