Anthony C.T. Liang. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 73-76). / Abstract --- p.1 / Chapter Chapter 1 --- General Introduction / Chapter 1.1 --- Introduction to antibiotics --- p.2 / Chapter 1.2 --- Use of antibiotics in antimicrobial chemotherapy --- p.3 / Chapter 1.3 --- Drug resistance in bacteria --- p.4 / Chapter 1.4 --- Genetic of infections drug resistance --- p.6 / Chapter 1.5 --- Clinical importance of drug resistance --- p.8 / Chapter 1.6 --- Resistance studies on a clinically- isolated Shigella Species --- p.9 / Chapter Chapter 2 --- Materials and Methods / Chapter 2.1 --- Culture media for bacteria growth --- p.10 / Chapter 2.2 --- Large scale plasmid preparation by CsCl density gradient centrifugation --- p.12 / Chapter 2.3 --- Minipreps of plasmid DNA by the alkaline lysis method --- p.14 / Chapter 2.4 --- Elution of DNA using the Geneclean Kit --- p.16 / Chapter 2.5 --- Transformation of plasmid DNA into E.coli DH5α --- p.17 / Chapter 2.6 --- Antibiotic sensitivity test and screening of resistance colonies --- p.19 / Chapter 2.7 --- Agarose electrophoresis of DNA --- p.20 / Chapter 2.8 --- Restriction and ligation --- p.21 / Chapter 2.9 --- Protocol for studying substrate profiles of aminoglycoside-modifying enzyme (AME) --- p.23 / Chapter 2.10 --- DNA sequencing using the T7 sequencing Kit from Pharmacia --- p.26 / Chapter Chapter 3 --- Antibiotic resistance studies on multiple resistant Shigella spp / Chapter 3.1 --- Introduction --- p.34 / Chapter 3.2 --- Conjugation and transformation experiment --- p.35 / Chapter 3.3 --- Extraction of plasmid DNA from Shigella 2731and transconjugant 14R525(2731) --- p.37 / Chapter 3.4 --- Discussion --- p.39 / Chapter Chapter 4 --- Cloning and characterization of beta-lactamase gene of Shigella2731 / Chapter 4.1 --- Introduction --- p.40 / Chapter 4.2 --- Cloning of the beta-lactam gene in Shigella2731 --- p.42 / Chapter 4.3 --- "Resistance pattern of El, E2, and S1" --- p.43 / Chapter 4.4 --- "Plasmid DNA extraction of El, E2, and S1" --- p.44 / Chapter 4.5 --- Restriction mapping of the plasmid pSFlOO --- p.47 / Chapter 4.6 --- Discussion --- p.51 / Chapter Chapter 5 --- Cloning and characterization of the aminoglycoside resistance gene / Chapter 5.1 --- Introduction --- p.53 / Chapter 5.2 --- Cloning of the aminoglycoside resistance genes --- p.56 / Chapter 5.3 --- Substrate profile studies on aminoglycoside- modifying enzyme (AME) activity on transformant G and S --- p.57 / Chapter 5.4 --- Subcloning of plamsid DNA from transformant S --- p.60 / Chapter 5.5 --- "DNA sequencing of fragments A, B, and C" --- p.65 / Chapter 5.6 --- Discussion --- p.68 / Chapter Chapter 6 --- Conclusion --- p.79 / Chapter Chapter 7 --- Reference --- p.73