Thesis (M.Ag.Sc.)--University of Adelaide, Dept. of Entomology, 1966. / Typescript. Includes bibliographical references.

Includes bibliographical references

Typescript Includes bibliographical references

Typescript Includes bibliographical references

Thesis (M.Ag.Sc.)--University of Adelaide, Dept. of Plant Pathology, 1965. / Typescript. Includes bibliographical references.

Thesis (M. Ag. Sc.)--University of Adelaide, Dept. of Entomology, 1968. / Includes bibliographical references.

Deladenus proximus (Neotylenchidae) is a nematode associated with pine trees and woodwasps, Sirex nigricornis (Hymenoptera). Previous to this study, little was known about the geographic distribution and variability of D. proximus. Herein I present information about their life cycle, pathogenicity, and variability. The life cycle is similar to that of other species of Deladenus in that it includes mycetophagous and entomopathogenic stages. Fertilized female nematodes penetrate siricid larvae and grow in the body cavity releasing thousands of larvae. These larvae invade the gonads, mycangia (sacs containing symbiotic fungus) and eggs upon metamorphosis of the host. Females oviposit infected eggs and spores of fungus (Amylostereum chailletii) into stressed trees, where nematodes mature and feed on the fungus, completing the life cycle. From 2009 to 2012 a total of 1,574 woodwasps were collected from Arkansas, Illinois, Louisiana, and South Carolina. Woodwasps were dissected and live nematodes were reared on cultures of A. chailletii and examined upon maturation. Reared nematodes were compared against type specimens of D. ipini and published descriptions of D. proximus. My study indicates prevalence varied across localities but every infected female wasp was sterilized, as indicated by presence of nematodes in the eggs. In addition I compared diagnostic characteristics of adult nematodes from each locality and found no significant difference in their size and structures. The nuclear loci 18S, 5.8S and ITS1 and 2 and the mitochondrial locus cox1 were amplified from each nematode. Nuclear DNA was invariable from all 4 locations and had 99% identity to the invasive species Deladenus siricidicola. Analysis of mitochondrial DNA showed more variability so these data were used to evaluate genetic structure across localities. Analysis of the cox1 data revealed 19 haplotypes and the absence of any geographic clusters or subpopulations. The lack of geographic structure may be due to the fact that each female wasp is infected with only one adult female nematode and therefore larvae within a wasp are siblings. With a generation time of 2 weeks these nematodes can have 20 generations without immigration or emigration, suggesting these nematodes are inbred. The pattern of transmission of this nematode and pathogenicity is similar to that of Deladenus siricidicola, which is used as a biocontrol against the invasive species Sirex noctilio. Experimental infections of Deladenus proximus in Sirex noctilio are recommended to test their viability as a biocontrol agent.

The Eurasian wood wasp, Sirex noctilio, is considered a secondary pest in its native range; however, it has caused significant economic damage when introduced to pine plantations in the Southern Hemisphere. Sirex noctilio was recently introduced to the northeastern U.S., which has raised concerns about its potential impact on Southeastern pine plantations. This research was conducted to understand how silvicultural management affects populations of a native wood wasp, Sirex nigricornis, a wood wasp with similar ecosystem functions as S. noctilio. Sirex nigricornis abundance was higher in un-managed pine plantations than in managed plantations, mixed, and old growth forests. Additionally, geospatial models were built displaying S. noctilio hazard for the Southeastern U.S. based on oviposition host preference assays and historical outbreak information. Sirex noctilio hazard models will inform land managers about areas of greatest concern under various scenarios and should be used to decrease susceptibility of pine forests to this pest.

The woodwasp Sirex noctilio is one of the most serious invasive pests of Pinus plantations in the southern hemisphere. Extensive control programs have been developed to manage this pest, of which biological control has been a major component. This thesis examined the factors that could influence the control of S. noctilio in South Africa. A critical comparison of S. noctilio infestations and control efforts throughout the southern hemisphere revealed that control has not been uniformly effective, and local adaptation of control strategies is likely required as S. noctilio moves to new areas. The parasitic nematode Deladenus siricidicola is considered the primary biological control agent of S. noctilio. This nematode also feeds on the fungal symbiont of S. noctilio, Amylostereum areolatum. Possible factors influencing the success of this nematode in the summer rainfall areas of South Africa were examined. Data from a field trial revealed that moisture content of the wood influences inoculation success and this is influenced by the time of inoculation and the section of the tree inoculated. Laboratory-based assays revealed that incompatibility between the strain of A. areolatum and D. siricidicola was unlikely to be the cause of low inoculation success with the nematode, but that artificial inoculations could be affected by competition of A. areolatum with sapstain fungi. The parasitic wasp Ibalia leucospoides is another biological agent for S. noctilio. Mitochondrial and nuclear DNA sequence data reflected the extensive introduction of I. leucospoides into the southern hemisphere, followed by genetic bottlenecks that fixed only a few haplotypes in the introduced populations. Promoting awareness of S. noctilio in the forestry community has also been an important component of the control strategy. Data from a survey questionnaire showed that the awareness campaign had been generally successful, but the lack of basic knowledge to identify S. noctilio and its symptoms and the poor reach of the awareness media to some sectors of the forestry community, was of concern. This thesis has contributed towards understanding the factors that influence the control of S. noctilio in South Africa, with relevance to other regions where S. noctilio has been introduced. / Thesis (PhD)--University of Pretoria, 2010. / Zoology and Entomology / Unrestricted