The major functions of glutathione S-transferase (Glutathione S-transferase; GST) include the maintenance of intracellular redox state of substances, and detoxification of cells of endogenous and exogenous toxic substances, and therefore GST plays an important physiological role. The main purpose of this study is to find the effects of SjGST and morin on human breast cancer cell on growth and migration , and the possible mechanisms. Therefore we first carried out cell proliferation assays in vitro . Results showed that at low concentrations of SjGST could enhance the proliferation of breast cancer cell line MDA-MB435s better then high concentrations.And the cell migration assays results showen, when 36 and 48 hours that the low concentration SjGST can enhance the breast cancer cell line MDA-MB435s migration. Morin have antioxidant,anti-free radicals, and inhibitor of cancer cells growth. Therefore, we aimed at revealing the effect of more, GST and the mixture of both on growth and detoxination of MDA-MB435s. However, the results of detoxification assays shown,even add SjGST first or last or both mixed ,the concentration 5¡B10¡B50¡B100¡B200¡B500nM of SjGST still not protect the breast cancer cells (MDA-MB-435s cells line) to break away from morin .

Glutathione S-transferase (GST) is an essential detoxification enzyme in eukaryotic cells, by catalysing the conjugation of electrophilic substrates to glutathione (GSH) and detoxification both in external and internal cellular enviornments. Previous studies of our team suggested that SjGST had profound on the growth of human breast cancer cells in vitro. In order to analyse the effect of SjGST on the proliferation and migration of human breast cancer cell line, MDA-MB 435s , we initially constructed plasmid containing gst fragment in pAcGFP-N1 and transfected into MDA-MB 435s cells. Assays for proliferation and migration of the transfected cells showed that no difference between transfected and non-transfected cells was found. Analysis of the transfected gene found a frameshift mutation occurred in the plasmid, and thus no expression was detedted. Thus, we carried out assays for proliferation and migration of the cancer cells using recombiation SjGST instead. Results from assays for proliferation and migration showed that SjGST enhance both proliferation and migration of MDA-MB 435s. Furthermore, the recombinant protein was a strong inhibitor to cell death, probably by detoxification pathway. But the mechanism of action of detoxification of shikonin by the recombinant protein, however remains unknow.

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