Background: Resistance training has proven to increase athletic performance, traditionally barbell training and Olympic Lifting have been used for this purpose. Sling training has recently been developed as a complement or substitution to traditional resistance training. Research has shown an increase in sport specific athletic performance and core stability with sling training. TRX Suspension Trainer is a newly developed sling training tool and to date no independent research has been done with the TRX. Purpose: To examine and compare muscle activation using TRX and the Olympic Lifting movement Hang Clean. Methods: 32 senior high school male soccer players participated in the study. Surface electromyographic (sEMG) data were collected on mm.erector spinae (back), m.gluteus maximus (glutes), m.vastus lateralis (quadriceps), m.semitendinosus (hamstrings) and m.gastrocnemius caput laterale (calf). Surface EMG data was collected when the subjects performed five different exercises, Hang Clean, TRX Squat Jump, TRX Front Squat and TRX Power Pull. In addition a Squat Jump was used as reference. Results: A similar muscle activation was found between Hang Clean (674 µV), TRX Squat Jump (684 µV) and TRX Front Squat (691 µV). TRX Power Pull showed the highest activation for mm.erector spinae and m.gluteus maximus but the lowest when comparing total muscle activation for all measured muscles. Conclusion: The similar amount of muscular activation for Hang Clean, TRX Squat Jump and TRX Front Squat indicates that the TRX Suspension Trainer can be used as a complement, for experienced athletes, or a substitution, for novice athletes, to traditional strength training. Coaches and athletic trainers should acknowledge the need and the importance of resistance training for athletic performance.