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The role of an imprinted microRNA in mouse developmentAllen, Sarah Elizabeth January 2013 (has links)
No description available.
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Light activated RNA interferenceShah, Samit, Friedman, Simon H. January 2007 (has links)
Thesis (Ph. D.)--School of Pharmacy and Dept. of Chemistry. University of Missouri--Kansas City, 2007. / "A dissertation in pharmaceutical science and chemistry." Advisor: Simon H. Friedman. Typescript. Vita. Description based on contents viewed July 16, 2008; title from "catalog record" of the print edition. Includes bibliographical references (leaves 206-220). Online version of the print edition.
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Comparison and improvement of siRNA design toolsMui, Yuen-chi. January 2004 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2005. / Title proper from title frame. Also available in printed format.
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The effect of the siRNA-mediated downregulation of the non-integrin laminin receptor on cancer cell viabilityMoodley, Kiashanee 08 August 2013 (has links)
A dissertation submitted to the Faculty of Science, University of the Witwatersrand,
Johannesburg, in fulfilment of the requirements for the degree of Master of Science.
Johannesburg, 2013 / Cancer is a hypernym used to describe a group of diseases characterised by the
uninhibited growth and spread of abnormal cells in the body. An estimated 7.6 million
annual deaths are attributed to the disease while 12.7 million new cases are reported
every year. The severity of this disease demonstrates the urgent requirement of novel
anti-cancer therapeutic agents. The non-integrin laminin receptor, here designated the
37 kDa/67 kDa laminin receptor (LRP/LR), is a multifunctional protein located on the
surface, in the cytoplasm, in the perinuclear compartment and in the nucleus of cells.
While this receptor is imperative for normal cellular functioning, it has also been
implicated in many diseases – it serves as a cell surface receptor for numerous
viruses, infectious prion proteins as well as certain respiratory tract pathogens.
Additionally, LRP/LR has been found to have some involvement in zoonotic diseases
and those involving neurodegeneration, such as Alzheimer’s disease. Most
importantly for this study, LRP/LR has been implicated in cancer progression, where
it was found to be overexpressed on the surface of various cancer cell lines, this
overexpression correlating to increased metastasis. The aim of this study was to
investigate the effect of the siRNA-mediated downregulation of LRP expression on
the viability of tumorigenic lung and cervical cancer cells (A549 and HeLa,
respectively). The cell surface LRP/LR and total LRP levels were investigated using
flow cytometry and western blotting, respectively, in A549 and HeLa cells, the results
revealing high percentages of both cell lines expressing LRP/LR on their surface.
Additionally, A549 and HeLa cells express similar total levels LRP. The
transfectability of these cells was confirmed and siRNA-LAMR1 was shown to
significantly downregulate LRP expression (80% and 60% in A549 and HeLa cells,
respectively). MTT assays revealed that the significant 13% and 18% reduction in
cellular viability in A549 and HeLa cells, respectively, was as a consequence of LRP
downregulation. This reduction in cellular viability was found to be a consequence of
induced apoptosis (identified by the visualisation of the loss in nuclear integrity, as
well as the significantly increased activity of the apoptosis-associated protein caspase-
3) and inhibited cellular proliferation in the aforementioned cells. These findings
suggest that siRNA targeting LRP mRNA may act as a potential alternative
therapeutic tool for the teatment of cancer.
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Synthetic RNA interference against influenza A virusLee, Hung-chiu., 李洪釗. January 2005 (has links)
published_or_final_version / abstract / Microbiology / Master / Master of Philosophy
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Filtering of false positive microRNA candidates by a clustering-based approachLeung, Wing-sze, 梁穎思 January 2009 (has links)
published_or_final_version / Computer Science / Master / Master of Philosophy
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The role of MicroRNA in 20(R)-ginsenoside-Rg3-induced anti-angiogenesisKeung, Man Hong 01 January 2010 (has links)
No description available.
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Role of microRNAs in ginsenoside-Rg1-induced angiogenesisChan, Lai Sheung 01 January 2009 (has links)
No description available.
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Epigenetic dysregulation of microRNA-9 (miRNA-9) in hepatocellular carcinoma (HCC)Tam, Hoy-kam, Aegean., 譚凱琴. January 2011 (has links)
published_or_final_version / Pathology / Master / Master of Philosophy
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A comparative study of circulating microRNAs in nasopharyngeal carcinoma patientsMan, On-ying., 萬安瑩. January 2012 (has links)
Nasopharyngeal carcinoma (NPC) is squamous cell carcinoma derived from the
epithelial layer of nasopharynx. The incidence is high in Southern China and
South-east Asia. In Hong Kong, the prevalent of NPC subtype is undifferentiated
NPC and is in close association with Epstein-Barr virus (EBV). MicroRNAs
(miRNAs) are small non-coding RNAs. They play vital roles in regulating gene
expression at post-transcriptional level. EBV also expresses viral miRNAs but the
function remains unclear. In NPC diagnosis and monitoring, circulating EBV
DNA level has been commonly used. However, in some cases, EBV DNA is
below the detection threshold in the plasma of NPC patients making it impossible
to be used in continuous monitoring of the patients. This study aimed to evaluate
whether miRNAs (both NPC-derived and EBV-derived miRNAs) could be used
as candidate circulating markers for disease monitoring.
Candidate gene approach was used to select suitable circulating miRNA markers
for NPC patients. Four candidate miRNAs including miR-21, miR-1301, miRBART7
and miR-BART22 were examined. The expression levels were first
validated in paired NPC tissues and normal counterparts. Furthermore, circulating
miRNA levels were evaluated in the plasma of NPC and normal individuals. To
examine the changes of miRNA in response to radiotherapy, changes of
circulating miRNA were monitored in 13 NPC patients before and after
radiotherapy. In addition, functional assay in cell proliferation was performed to
validate the potential role of the candidate miRNA in the pathogenesis of
undifferentiated NPC.
Of the 4 candidate miRNAs, miR-BART7 was consistently over-expressed in
both tumor tissues and plasma samples of NPC. In addition, circulating miRBART7
was also detected in NPC patients in case of the plasma EBV DNA levels
below the detection threshold. In response to radiotherapy, 10 of 13 (76.92%)
patients had decreasing circulating miR-BART7 in the plasma samples collected
at 3 month after radiotherapy. Furthermore, introducing miR-BART7 mimics into
the undifferentiated NPC cell line HONE1 and normal nasopharyngeal-derived
epithelial cell cultures NP69 and NP460 resulted in significant increases in cell
proliferation rates of all the 3 cell lines.
To summarize, miR-BART7 expression was significantly higher in NPC patients
as a potential oncogenic miRNA. Evaluating the miR-BART7 levels is a possible
screening approach in NPC diagnosis and post-treatment monitoring. The
oncogenic role of miR-BART7 in the development of undifferentiated NPC
deserves further investigation. / published_or_final_version / Surgery / Master / Master of Philosophy
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