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The radiotracer methodology was combined with the Hoagland solution culture of growing soybean in a greenhouse to investigate the absorptions of phosphorus (P), calcium (Ca), and sodium (Na) as a function of salinity. Salt stress was varied by using zero to 120 mM NaCl. The research was initiated because of a need to increase soybean production in the saline soils of the semi-arid regions of the world. Although P absorption increased with time at each concentration of NaCl, increasing its concentrations ([NaCl]) to 120 mM reduced P uptake considerably. The addition of inorganic P (Pi) to the salt medium improved P absorption significantly (P < 0.0001) in stem, petiole, and roots. Polynomial regressions showed the relationship between 22Na activity and [NaCl] for leaves and petiole to be cubic (R2 = 1) while in the stem a quadratic relationship prevailed. A maximum of P and Na absorption was observed at 40 mM NaCl. The relationship between 32P activity and increasing [NaCl] was linear for the roots (a positive slope) and the stem (a negative slope). 45Ca and 32P dual labelling part of the experiments failed to produce results because an unexpectedly high degree of tissue quenching which prevented from obtaining the minimum counting requirements for separation. Shoot fresh and dry weights decreased linearly with increasing [NaCl] as did the root fresh and dry weights. Leaf chlorophyll content during the last week of the final harvest showed a linear relationship with time. Chlorophyll increased with time linearly when the growth medium contained zero and 40 mM NaCl; whereas a negative slope was obtained for 80 and 120 mM NaCl. It seems that P fertilization of the soil could ameliorate the salt effect. 22 Na uptake results indicated that there is a mechanism for exclusion of Na from soybean plant parts.

Over 10% of the world’s land is salt affected. Salt accumulation is a major soil constraint for agricultural sustainability in arable or newly cultivated soils. As a result of salinity, soil chemical, physical and biological properties deteriorate, plant uptake of water and nutrients, particularly P, decreases and plant growth declines. Application of plant residues can enhance the activity of soil microorganisms, the availability of nutrients, including P and the plant uptake of P and growth. Such a practice can also be economically viable as it can reduce the use of P from inorganic sources, maintaining the world’s reserve of P rocks and reducing the price of fertilizers and the environmental pollution often associated with the excessive application of inorganic N and P fertilizers. Little is known about how P, with N in proper form, added from inorganic and/or residue sources can affect wheat growth in the salt affected soils with no confounding pH or sodium adsorption ratio (SAR). Increasing microbial activity, N and P availability and wheat uptake of P by application of N and P from organic and inorganic sources may improve wheat growth and hence productivity under saline conditions. The overall aim of this study was to determine ways for enhancing the activity of microorganisms and increasing the availability of N and P, the uptake of nutrients, particularly P and the growth of wheat by management of fertilization from inorganic and organic sources in saline soils. This study therefore was conducted with the following aims: 1) to investigate the relationship between salinity and P availability; 2) to assess wheat response to combined application of N and P fertilizers under saline conditions; 3) to evaluate the effect of plant residue addition on N and P availability and microbial activity in salt affected soils; 4) to determine microbial response to addition of inorganic N rate and form, and how this will affect N and P availability in a saline soil, and 5) to determine the effect of P added from inorganic fertilizer and plant residue, compared to inorganic P fertilization, on microbial biomass and wheat nutrient composition and growth in a saline soil. In saline soils, P availability can be affected by the salt type and concentration and soil texture. Three experiments were conducted to study the relationship between P availability, soil texture and salinity. The results of the first experiment in which soil was shaken with different concentrations of NaCl or CaCl2 or Na2SO4, indicated that P solubility decreased with increasing concentration of Ca2+, but was not affected by Na+ salts. In the second experiment, P availability (after 24h shaking) decreased with increasing salt concentration up to EC1:5 3.1 dS m-1, increased with increasing P addition (0, 100, 200, 400, 600, 1200, 2500 and 5000 µg P g-1 soil), and was generally higher in sandy soil than in sandy loam soil. In the third experiment (15 days incubation), it was found that P availability significantly decreased one day after P addition which was followed by a further decrease to day 5, but then remained unchanged until day 15. It can be concluded that P availability is reduced in presence of clay, and decreases with increasing concentration of salts, particularly Ca2+, and that the availability of P stabilizes in sandy and sandy loam soils within 2 weeks after addition of P from inorganic source. Increasing N or P fertilization enhanced wheat growth in salt affected soils. Therefore combined application of N and P may enhance wheat growth in saline-non sodic soils with neutral pH. Three glasshouse experiments were carried out with the aim to determine the salinity range to be used in the subsequent experiments and to test the hypothesis that combined addition of N and P fertilizers can enhance wheat growth in a sandy loam soil with low SAR and neutral pH. The first two experiments were conducted in a sandy loam salinized to EC1:5 of 0.18, 1.36, 2.00 and 2.67 dS m-1 using NaCl and CaCl2. The wheat varieties Janz and Krichauff died in all soils to which salt was added showing that these EC levels were too high. The third experiment was conducted with Krichauff in the sandy loam soil with EC1:5 0.19, 0.32, 0.49, 0.67 and 0.86 dS m-1, equivalent to ECe 2.2, 4.4, 6.7, 9.2 and 11.8 dS m-1, respectively, and with 0, 30 and 60 mg P kg-1 soil and 50, 100 and 200 mg N kg-1 soil. Salinity reduced plant dry matter at all N and P application rates. Increasing N application rates decreased growth at low and high salinity, whereas increasing P addition improved growth at all salinity levels. The highest shoot and root dry weights were obtained with 50 mg N and 60 mg P kg-1 soil. Nitrogen and P fertilization did not increase wheat growth in soil with greater than EC1:5 0.67 dS m-1, equivalent to ECe 9.2 dS m-1. Plants are known to respond differently to N form. A glasshouse experiment was carried out to assess the effect of N form (NH4 +, NO3 - or NH4NO3) added at 50, 100 and 200 mg kg-1 soil, in addition to the control (no N), on nutrient composition and growth of Krichauff in a sandy loam soil with EC1:5 0.21, 0.48 and 0.86 dS m-1, equivalent to ECe 2.8, 6.6 and 11.8 dS m-1. Increasing soil salinity decreased shoot and root dry weights and shoot macro- and micronutrient concentrations with all forms of N. At every N addition rate and with increasing N addition from N50 to N200, compared to NH4 +, the salinity of soil solution was far higher with NO3 - and lowest with NH4NO3. Shoot and root dry weights were highest with addition of 50 mg NO3-N or 100 mg NH4-N or as NH4NO3 at all salinity treatments. Concentrations of shoot P, Fe, Mn and Zn concentrations were greater with NH4 + and NH4NO3 compared to NO3 -, but concentrations of shoot K and Ca were higher with NO3 - than with NH4 + nutrition at all salinity treatments. At a given N rate, shoot and root dry weights were greatest with NH4NO3 in the saline sandy loam soil with up to EC1:5 0.67 dS m-1. Two experiments were conducted to evaluate the effect of plant residue addition on microbial activity and biomass, and N and P availability in salt affected soils. Although the same amounts of Na+ and Ca2+ salts, EC1:5 differed between tested soils due to differences between soils in clay content and water holding capacity. The first experiment aimed to assess the salinity range for microbial activity over 2 weeks in saline soils with different texture amended with glucose/nitrate (C/N ratio 16:1). The EC1:5 were 0.2, 1.26, 1.83, 2.28 and 2.99 dS m-1 in the silty loam, 0.16, 1.10, 1.98, 2.33 and 3.18 dS m-1 in the sand and 0.19, 0.82, 1.75, 2.03 and 2.79 dS m-1 in the sandy loam. Soil respiration significantly decreased with increasing salinity in the glucose/nitrate amended soils, but was not completely inhibited even at highest salinity treatment. Cumulative CO2-C increased over 2 weeks and was highest in the silty loam soil and decreased in the following order: silty loam soil < sandy loam soil < sandy soil. The second experiment was conducted to determine the effect of three different plant residues added at 2% (w/w) on microbial biomass and N and P availability over time (70 days) in saline sandy and sandy loam soils with low SAR and neutral pH. The EC1:5 was 0.21, 1.08, 1.90, 2.63 and 2.89 dS m-1 in the sand and 0.19, 0.87, 1.63, 2.32 and 2.49 dS m-1 in the sandy loam. Microbial biomass C, N and P decreased with increasing soil salinity and were highest on day 10. With residue addition, microbial biomass C and P were significantly higher in the sandy than in the sandy loam soil, whereas no significant differences were found between soils for microbial biomass P at all salinity treatments. Under all salinity treatments, compared to other residues, highest biomass N was found in canola-amended sandy loam and in lupin-amended sandy soils. With increasing soil salinity, highest microbial P was found in the sandy soil amended with lupin residue. Nitrogen availability was generally higher in the sandy soil than in the sandy loam soil, whereas the opposite was found for P availability. Compared to canola and lucerne, N and P availability were highest in lupin amended sandy and sandy loam soil. Two experiments were conducted to assess whether N addition (rate and form) can affect the microbial activity in presence of residues in a saline sandy loam soil. The first experiment aimed to evaluate the effect of N rate (0, 25, 50 and 100 mg N kg-1 soil) added as NO3 - on soil respiration over 2 weeks under non-saline conditions in presence of 2% lupin residues. The second was to determine the effect of N added at 50 mg N kg-1 soil as NH4 + or NO3 - and lupin residue added at 2 and 4% (w/w) on microbial activity and biomass and N and P availability over 45 days in a sandy loam soil with EC1:5 0.21, 0.51 and 0.85 dS m-1, equivalent to ECe 2.8, 7.0 and 11.7 dS m-1. Soil respiration and cumulative respiration were not significantly affected by N application rate over 2-week-incubation under non-saline conditions. Microbial biomass and N and P availability decreased with increasing salinity and were highest at 4% lupin residue. Soil respiration rate and cumulative CO2-C and microbial biomass C, N and P were greater with addition of 50 mg N kg-1 soil as NO3-N compared to NH4-N at every addition rate of lupin residues under saline conditions. Soil microbial biomass C, N and P were highest on day 15 and decreased over time, whereas N and P availability were lowest on day 15 and increased over time. Since addition of inorganic N and P fertilizers improved the growth of wheat (cv Krichauff) in the saline sandy loam soil at SAR 1 and neutral pH, two glasshouse experiments were conducted to determine the effects of plant residue addition on the nutrition of wheat. The first experiment was conducted under non-saline condition to determine the effect of lupin residue rate (2% and 4% w/w) on wheat growth. The second experiment was conducted under saline conditions to determine the effect of P added as lupin residue (2%) and/or KH2PO4 (0, 20 and 40 mg P kg-1 soil) with and without 50 mg N kg-1 soil added as (NH4)2.SO4 on microbial biomass, N and P availability, plant growth and nutrient composition in the saline sandy loam soil. The EC1:5 were 0.23, 0.35 and 0.51 dS m-1, equivalent to ECe 3.1, 4.8 and 7.0 dS m-1, respectively. In the first experiment under non-saline conditions, shoot dry weight was lower with addition of 4% than with 2% lupin residue with and without inorganic N. In the second experiment under saline conditions, microbial biomass C and N increased with increasing application of inorganic P, but was not as much as in presence of lupin residues. In presence of lupin residue, wheat growth increased with increasing addition of inorganic P under saline conditions. Compared to the soil with P from inorganic fertilizer and residues, inorganic P increased shoot and root dry weights and shoot P, K, Mn and Zn concentrations, but not N concentration. Addition of 50 mg inorganic N in presence of lupin residues significantly increased N and P availability and microbial biomass, but had no significant effect on wheat growth in a saline sandy loam soil. The results showed that optimal application of N and P organic and inorganic fertilizers can improve N and P availability, microbial activity and wheat growth in salt affected soils. Highest wheat dry weight was achieved by application of 60 mg P kg-1 soil in a sandy loam soil with EC1:5 0.67 dS m-1, equivalent to ECe 9.2 dS m-1. Wheat growth was also improved with application of N-NH4 + or as NH4NO3 at 100 mg N kg-1 soil. These N and P fertilization rates can potentially enhance wheat growth in the sandy loam soil with up to EC1:5 0.67 dS m-1, but with SAR 1 at neutral pH. Plant residues increased microbial activity and N and P availability in the saline soils. In the soils used here, with residue addition wheat growth was P limited due to competition with microorganisms for available P. Therefore application of residues with inorganic P is necessary to satisfy wheat requirements of N and P in the saline sandy loam soil. In the saline sandy loam soil at SAR 1 and neutral pH, application of 2% lupin residues and 40 mg KH2PO4-P kg-1 soil achieved highest microbial biomass, nutrient availability and wheat growth. However, wheat growth with these rates is not as high as with inorganic P at similar rate due to micronutrient deficiency in the saline soil with lupin residues. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1331419 / Thesis (Ph.D.) -- University of Adelaide, School of Earth and Environmental Sciences, 2008

Thesis (M.S. - Soils, Water and Engineering)--University of Arizona. / Includes bibliographical references.

Thesis (Ph. D. - Soils, Water, and Engineering)--University of Arizona. / Includes bibliographical references.

Thesis (M.S. - Agricultural Chemistry & Soils)--University of Arizona. / Bibliography: leaves 53-55.

Thesis (M.S.)--Montana State University--Bozeman, 2004. / Typescript. Chairperson, Graduate Committee: James W. Bauder. Includes bibliographical references (leaves 67-73).