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The construction and use of interspecific somatic cell hybrids in reverse genetics an approach to the analysis of the mouse genome with special reference to the mouse chromosome 17 /Höglund, Mattias. January 1992 (has links)
Thesis (doctoral)--Lund University, 1992. / Added t.p. with thesis statement inserted.
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Somatic embryo development and phenotypic variation in an abscisic acid-independent line of Larix x eurolepisHay, Elizabeth Irene 02 August 2018 (has links)
The objectives of this thesis were to trace the developmental pathways of somatic embryos
of an abscisic-acid independent line of Larix x eurolepis. to catalogue the phenotypes of mature
embryos, to determine critical stages of development and to attempt to increase the number of
maturing somatic embryos. The low rate of maturation could not be entirely explained by
differences in phenotypes of early embryos, critical stages of development, or the lack of plant
growth regulators in the medium. In addition, the shape and epidermal type of the mature embryo
did not always determine the type of epicotyl produced, nor did it affect the rooting and mortality
rates. Six types of embryonal structures were evident in the aggregates of line 2086: (1) a smooth
(SEMLS) or (2) rough (REMLS) embryonal mass subtended by a cylindrical, compact, long
suspensor. (3) a rough embryonal mass subtended by a long, loose suspensor (REMLLS). (4) a
rough embryonal mass subtended by a suspensor arising from greater than one quarter of the
surface area of the embryonal mass (REMST). (5) a rough embryonal mass subtended by a short,
compact, cylindrical suspensor (REMSS). and (6) a cluster of meristematic cells which may or may
not have single suspensor cells attached (MC). For isolated embryonal structures of all types, to
continue development into a nodule or a mature embryo was the least common fate, while
proliferation and developmental arrest were more common. In general, the more organized
embryonal structure types (SEMLS and REMLS) had higher rates of maturation compared to the
other 4 types but the most common fate was still developmental arrest (74% SEMLS. 62%
REMLS), followed by proliferation (10% SEMLS. 30% REMLS), and nodule or embryo
development (16% SEMLS. 9% REMLS). REMLLS and REMST embryonal structures became
developmentally arrested or proliferated (43-47%) while the rate of nodules/mature embryos
production was 9-11%. Neither individual REMSS nor MC structures produced any nodules or
mature embryos, but REMSS had a lower rate of developmental arrest (81%) and a higher rate of
proliferation (19%) than MC (89% and 11% respectively). Embryos at more advanced stages of
development were less likely to die, become developmentally arrested or become nodules, but more
likely to become mature embryos than embryos at less advanced stages of development. A critical
stage of development appeared to be the focal zone stage at the formation of a complete polyphenol
band around the basal end of the embryonal mass. At this stage, the majority of immature embryos
became mature embryos (61%) while only 3% of the embryos died. 10% became developmentally
arrested, and 20% became nodules. The majority of mature somatic embryos were normally
proportioned with a smooth epidermis (43%) rather than vitrified (12%). normal with a rough
epidermis (12%) or misshapen (smooth or rough. 33%). The shape of the mature embryo was
associated with the type of epidermis, with mature somatic embryos with normal proportions more
likely to have smooth epidermis (78%) than a rough epidermis (22%) while mature embryos with
abnormal proportions were as likely to have a smooth epidermis as a rough epidermis. The shape
of the mature embryo was associated with the shape of the epicotyl produced. Normal-smooth,
mature embryos were more likely to produce normal-smooth epicotyls (73%) than twin epicotyls
(21%), vitrified epicotyls (2%) or misshapen epicotyls (5%) compared to vitrified mature embryos
(42% normal-smooth epicotyls, 34% twin epicotyls, 23% vitrified epicotyls, 1% misshapen
epicotyls) or misshapen mature embryos (22% normal-smooth epicotyls, 47% twin epicotyls, 7%
vitrified epicotyls, 24% misshapen smooth/rough embryos). The number of mature embryos
which germinated or died was not associated with either the epidermal quality or the shape of the
mature embryo. Few SEMLS or REMLS embryonal structures responded to auxin and cytokinin
treatments. There appeared to be a trend towards less developmental arrest and proliferation and
more nodules/mature embryos produced on media with no auxin compared to media with 2,4-D
and a trend towards more developmental arrest and fewer nodules/mature embryos on media
without BA compared to media with BA. Only nodules on media without plant growth regulators
produced roots or cotyledons. There was no effect of embryonal structure type (SEMLS or
REMLS), or sucrose concentration (58 μM or 174 μM) on the maturation of immature embryos,
but on media without ABA, fewer immature embryos proliferated or became developmently
arrested and more embryos became nodules or mature embryos than on medium with 6-24 μM
ABA. / Graduate
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Somatic Embryogenesis of Magnolia spp. and CultivarsPlotke, Kathryn January 2018 (has links)
This study focused on induction of somatic embryogenesis of Magnolia spp. and cultivars utilizing leaf and seed (immature and mature) tissues with attempted micropropagation experiments. In a preliminary experiment, direct embryo regeneration was successful in a single leaf tissue of M. ‘Yellow Bird’. After various micropropagation experiments, microshoot proliferation rates decreased. As a result of minimal leaf material, mature seeds were utilized but had contamination issues. Subsequent experiments utilized immature seeds. M. ‘Leonard Messel’ and M. stellata had significantly greater embryo regeneration rates and M. ‘Rosea’, M. stellata, and M. kobus had greater callus induction rates. Woody Plant Medium had significantly greater rates of embryo regeneration as compared to Yellow Poplar medium. Further experimental measures including various collection times of immature seeds are necessary for an efficient regeneration protocol to support potential research utilizing floral-inducing genes to induce rapid breeding cycles for selection of magnolias with diverse floral characteristics.
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Psychopharmakologische Behandlung stationärer Patienten mit somatoformen Störungen - Veränderungen über die letzten zwei Jahrzehnte / Pharmacotherapy of hospitalized patients with somatic symptom disorder - treatment changes within the last two decadesHuber, Julia Martha 06 March 2018 (has links)
No description available.
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Mind, body, and choice: A review of alexithymia and the somatic-marker hypothesisSnellman, Henrik January 2022 (has links)
This paper examined the claims postulated by the somatic marker hypothesis and compares those claims to the current evidence surrounding the neural basis of alexithymia. The results were then compared to see if they contradict or have a distinct localisation in the brain separate from those behavioural brain regions hypothesized by the somatic marker hypothesis. It was concluded that the somatic marker hypothesis and the neural basis for alexithymia share certain regions of interest, primarily the amygdala and insula, but also potentially the anterior cingulate cortex.
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Relationships between milking machines, speed of milking and somatic cell count level in dairy cowsMoore, Robert Kevin. January 1980 (has links)
Note:
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Neuropsychological Correlates of Risk-Taking Behavior in an Undergraduate PopulationTsanadis, John January 2005 (has links)
No description available.
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Micropropagation of date palm (Phoenix dactylifera L.) and papaya (Carica papaya L.)McCubbin, Michelle Jacqueline. 19 December 2013 (has links)
Date palms (Phoenix dactylifera L.) and papayas (carica papaya L.) are two
commercially important plantation crops. Their economic potential in South Africa
and worldwide is increasing. However, due to disease, pests and socio-economic
reasons, planting material is in short supply. Micropropagation provides a method for
rapidly propagating selected superior cultivars for commercial and environmental interests.
A satisfactory process for the regeneration of elite cultivars should result in
individuals phenotypically and genetically identical to the explant from which they
were derived. However, due to somaclonal variation generated during in vitro
culture, the true-to-typeness is questionable. For this reason a southern African
survey for off-types on date palms produced using somatic embryogenesis was
conducted. Plant growth variations such as leaf variegation, seedless fruit, broad
leaves, compact growth habit and parthenocarpic fruit were recorded and possible explanations for each phenomenon given.
Factors influencing the date palm initiation process such as decontaminating agents,
plant growth regulators, explant type and nurse cultures were investigated. A double
decontamination process with 2.6% and 1.3% sodium hypochlorite was most
effective at reducing contamination. Alternative plant growth regulators, TIBA and
NAA were ineffective as a substitute to 2,4-D for somatic embryogenesis. The size of
the explant and "nurse cultures" played an important role in explant growth and
initiating callogenesis. A "nurse culture" reduced the time in culture significantly. The
problem areas in the three commercial tissue culture techniques used for date palms were outlined.
In the second part of the study, factors influencing initiation, multiplication and
rooting of papaya were determined. Presoaking with antibiotic, Rifampicin, and
various fungicides had a positive effect on decontaminating papaya explants, while
Bronocide™ had little effect. Various methods and materials were used to optimize
papaya multiplication and rooting in vitro. The growth and multiplication of papaya
was optimal at 50 g l ¯¹ sucrose. Gelling agent, Gelrite, increased multiplication rates significantly but had a negative effect on overall growth causing plants to become
vitrified. The addition of activated charcoal reduced vitrification but also reduced
multiplication rate. Activated charcoal greatly improved overall growth of papaya and
reduced leaf senescence. No vitrification was observed in multiplying papaya cultures
where agar and Gelrite combinations were used, but multiplication rate was reduced
compared to cultures grown on Gelrite alone. callus removal from the bases of
papaya at subculturing reduced multiplication rate and influenced elongation, growth and leaf senescence.
Lower concentrations magar and Gelrite improved rooting percentages, but did not
provide good support. Damaged roots and lower rooting percentages were observed
on plantlets treated with IBA for four weeks compared to those exposed for only two
days. A one hour pulse with a higher concentration (5 mg l ¯¹)
of IBA greatly
improved rooting percentage and further eliminated a second subculture onto an
IBA-free medium after two days. Good, strong roots with root hairs were produced
on vermiculite medium containing equal volumes of DS salts and vitamins. Modified lids with cotton-wool plugs also reduced leaf abscission.
In vitro grafting using stericrepe proved impractical, while grafting in vitro unrooted
papaya plants onto ex vitro seedlings was more successful, using wedge and slant
grafts. Grafts sealed with pegs and Parafilm™ were less effective. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 2000.
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Emotional Awareness and Psychophysiological Markers of Performance on the Iowa Gambling TaskInman, Cory 07 February 2007 (has links)
The present study examines the relationship of emotional awareness to anticipatory psychophysiological markers and performance on the Iowa Gambling Task (IGT). The IGT is a computerized card game that simulates real-life decisions through uncertainty of reward or punishment. The participant’s goal is to make advantageous card choices. Anticipatory somatic markers of physiological arousal, like electrodermal activity and heart rate, have been proposed to bias decisions in the IGT. The central hypothesis is that a participant’s emotional awareness is related to their ability to make advantageous decisions through biasing psychophysiological responses. The Toronto Alexithymia Scale was used to assess each participant’s emotional awareness. Less emotional awareness was associated with enhanced performance on the IGT. However, anticipatory physiological arousal (electrodermal activity and heart rate) and emotional awareness yielded no significant relationships. Findings suggest a need for further research on cognitive models, such as the expectancy valence model, in relation to decision-making.
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Induction Of Embryogenic Tissue And Development Of Somatic Embryos In Pinus Brutia Ten.Yildirim, Tolga 01 July 2005 (has links) (PDF)
Conifer species are subjected to major time constraints in tree improvement because of their long regeneration cycle and large sizes. However, integration of developing biotechnologies could significantly reduce this time limitation in tree breeding programs. In this regard, somatic embryogenesis (SE) offers a great potential in commercially important Turkish red pine (Pinus brutia TEN.) for rapid production of larger number of clones as well as capture of greater genetic gains. In this study, seven collections were done to sample precotyledonar zygotic embryos for induction of embryogenic tissue (ET) from 15 clones located in Antalya. Afterwards, abscisic acid, carbohydrates, polyethylene glycol (PEG), and gellan gum were tested to obtain mature somatic embryos in maturation experiments. Analyses of variance showed a significant variation among collection dates (43.1% of total variance) and clones studied (18.8% of total variance) for induction of ETs. Overall initiation frequency of ET in this study was 11.6% with clonal range of 4.7 & / #8211 / 24.1%. Of those tested maturation treatments, 80& / #956 / M ABA, sucrose and maltose at 3 and 6%, 3.75% PEG combined with 1% gellan gum were found to be suitable for maturation of somatic embryos in Turkish red pine. Sixty nine somatic embryos were obtained from Clone 22, which was one of tested clones. Induction frequencies could be further improved by using different basal media and/or manipulating media components, such as plant growth regulators. For proper maturation of somatic embryos, embryogenic lines need to be screened to find suitable lines, which are developmentally responsive to ABA treatment.
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