UA Open Access Publishing Fund / We examined a secondary contact zone between two species of desert tortoise, Gopherus agassizii and G. morafkai. The taxa were isolated from a common ancestor during the formation of the Colorado River (4–8 mya) and are a classic example of allopatric speciation. However, an anomalous population of G. agassizii comes into secondary contact with G. morafkai east of the Colorado River in the Black Mountains of Arizona and provides an opportunity to examine reinforcement of species’ boundaries under natural conditions. We sampled 234 tortoises representing G. agassizii in California (n = 103), G. morafkai in Arizona (n = 78), and 53 individuals of undetermined assignment in the contact zone including and surrounding the Black Mountains. We genotyped individuals for 25 STR loci and determined maternal lineage using mtDNA sequence data. We performed multilocus genetic clustering analyses and used multiple statistical methods to detect levels of hybridization. We tested hypotheses about habitat use between G. agassizii and G. morafkai in the region where they co-occur using habitat suitability models. Gopherus agassizii and G. morafkai maintain independent taxonomic identities likely due to ecological niche partitioning, and the maintenance of the hybrid zone is best described by a geographical selection gradient model.

<p><italic>Tentative Embrace</italic> is a composition in five movements for flute, clarinet, saxophone quartet, vibraphone, piano, string quartet and tape track. The tape track features spoken text and soundscape recordings gathered in and around the Sonoran desert of the Southwestern United States. The text and the soundscape recordings, along with the composed music, are all my own work. </p> <p>This piece embodies my efforts to interpret and translate the complicated sensation of being a human alone in nature - of wanting to belong, realizing I do belong, but also not quite belonging. The Sonoran desert, the site of inspiration for this work, is an especially revelatory space that heightens these simultaneous sensations of connection and disconnection; it draws attention to the biological points of contact between human beings and their natural surroundings, but it also emphasizes those cultural and material differences that we carry with us into such a space. Through the combination of the music, the text and the soundscapes, I work to convey the ever-shifting boundaries between the self and everything else. </p> <p>For the music, I find formal inspiration in the slow and cyclical pace of the desert itself; musical ideas unfold gradually through ever-varying repetitions. Each movement is devoted to a particular phenomenon experienced in the desert, and while the text and the soundscapes work to articulate the specifics of these phenomena, the music gives form to their structural and sensual suggestion. I move back and forth between specifics and abstractions; as such, some of my translations of this space will be more audible than others, but each of them demonstrate this attempt at forging an artistic point of connection with this environment.</p> / Dissertation

No description available.

One of the important contributions genetic studies have made to conservation is the ability to resolve taxonomy and define relationships among populations. However, this can be complicated when species exhibit hybridization. Hybridization can be an important part of the evolutionary process and a critical component in a species ability to adapt to a changing environment. Most hybrid zones are observed at ecotones between two distinct habitats and this may be important in defining the role of hybrid zones in the evolutionary process. I examined hybridization among the three distinct lineages of desert tortoises in the genus Gopherus. An important aspect of this study system is the presence of areas of overlap between divergent lineages of desert tortoise which allowed me to test hypotheses about which forces influence these taxonomic boundaries. Specifically, I tested hypotheses about the contribution of physical vs. ecological segregation and the relative importance of isolation and gene flow in the formation of these disparate desert tortoise lineages. I used mtDNA sequence data and 25 microsatellite loci to perform Bayesian clustering, clinal analyses and habitat suitability modeling to infer population structure and influence of landscape features at each contact zone. In both instances, I observed ecological niche partitioning and limited hybridization at ecotones. I then used mtDNA and four nDNA loci to perform a multi-locus phylogenetic analysis to estimate the species tree among desert tortoise lineages and tested for ancestral admixture with RNA-seq data using demographic inference employed in the software package ∂a∂i. My results validate taxonomic distinction among all three lineages without evidence of ancestral introgression. These data suggest that despite the presence of contemporary hybridization and incomplete reproductive isolation, divergence among these lineages is consistent with species-level differentiation. By clarifying the evolutionary processes that influence the distribution of desert tortoise lineages, this study will directly inform efforts to preserve the evolutionary potential of these threatened species. Ultimately, understanding the evolutionary history of desert tortoises not only clarifies the forces that have driven speciation in this group, but it also contributes to our knowledge of the biogeographic history of the southwestern deserts and how diversity is maintained within them.

No description available.

I quantified the bat assemblage associated with a Sonoran Desert riparian corridor at a wildland-urban interface using mist netting (2002-2005) to assess differential spatial and temporal resource use. My capture rate was high (17 species and 961 individuals) considering the aridity of the area; however, landscape complexity of this montane region undoubtedly contributes to foraging opportunities. I found that bats were distributed along the canyon when water was plentiful but their activity was concentrated at isolated pools during dry periods. I also found temporal variation in pool-use by the most frequently captured species. I conducted an acoustic study to measure bat-use between deciduous riparian and Sonoran desertscrub communities. I measured activity levels using number of acoustic call files. I found greater bat foraging in desertscrub and used a multidisciplinary approach to determine why bats might use the more arid environment. All capture data and supporting analyses are included in appendices.

Sonoran Desert Drosophila mettleri breeds in soil soaked by the necrotic cacti juices from saguaro (Carnegiea gigantea) and cardon (Pachycereus pringlei). An isolated population on Santa Catalina Island, 300 kilometers NW of the Sonoran Desert limit, was discovered breeding in several Opuntia cacti species. Host shifts are associated with the speciation process in phytophagous insects. I tested for evidence of premating isolation, postmating isolation, and ecological differences among allopatric populations of Drosophila mettleri using a variety of approaches. No sexual isolation was detected. However, Drosophila mettleri from Santa Catalina Island shows significant behavioral and physiological differences compared with Sonoran Desert populations. Furthermore, Drosophila mettleri from Santa Catalina Island was significantly genetically differentiated from all other populations in the study. Finally, I observed sufficiently significant F1 male sterility in crosses involving the Santa Catalina Island population to consider it indicative of early postzygotic isolation.

abstract: This study identifies the flora of the Eagletail Mountain Region, an area covering approximately 100,600 acres, located in west-central Arizona that includes the Eagletail Mountains, Granite Mountains, portions of the Harquahala Valley, and Cemetery Ridge near Clanton Well. The region is located about 129 km (80 mi) west of Phoenix and 24 km (15 mi) south of Interstate 10. Plants were collected over a six-year period, beginning September, 2004 and ending May, 2010, including two wet winters and two wet summers. A total of 702 collections were made covering 292 species that represented 63 families. Additional information on the region included in the thesis are: 1) an analysis of the climate, based on 20 years of rainfall records; 2) a description of the geology and its influence on plant distribution; 3) a prehistory and history identifying archeological sites; 4) an analysis of food plants used by the Native Americans that suggests how they were able to live in the region; 5)a paleo-botanical history based on an evaluation of pack-rat midden collections from mountain ranges around the region; 6) a comparison of the trees, shrubs, and perennials of the Eagletail Mountain Region with those of the Sierra Estrella and Kofa Mountains; and 7) a survey of non-native species. The habitats that the plants occupied based on climate and soils included were: 1) the bottoms and sides of sandy/ gravelly washes, 2) bajada slopes-volcanic soils, 3) bajada slopes-granitic sandy soils, 4) slot canyons/rock outcrops, 5) desert pavement, and 6) open valleys. Each habitat has its own characteristic species composition and distribution. / Dissertation/Thesis / M.S. Biology 2012

Adding a surface rock layer (also called rock armor or rock mulch) to constructed slopes improves erosion resistance but has had mixed effects on revegetation. This study investigated the effects of rock layer depth (no rocks, 10-, 15-, and 20-cm rock layers) and rock size (5-20cm diameter rocks) on vegetation cover. Seeding was applied four times in the first 2 years. After 3 years, plots with a rock layer averaged 7% vegetative cover compared to 85% on plots without a rock layer. There was a nonsignificant trend toward less vegetation with a deeper rock layer.

Coryphantha scheeri var. robustispina (Schott) L. Benson, the Pima pineapple cactus, herein referred to as C. scheeri, was declared an endangered species in 1993 and only occurs in a limited range in Arizona, USA and northern Sonora, Mexico between 2,300'-4,500' asl. Development within the range of C. scheeri threatens individuals, but transplanting to conserve them while allowing for development has been considered to be ineffective for conservation due to low post-transplant survival rates in past studies. The construction of a natural gas pipeline provided the opportunity to conduct a transplant experiment on 82 individual C. scheeri transplanted in July and August 2014. The plants were randomized into one of four transplant methods: bare-root with supplemental water, bare-root without supplemental water, soil-and-plant moved with supplemental water, and soil-and-plant moved without supplemental water. Higher than average precipitation occurred during the 2014 monsoon season including after transplanting. A subset of the transplanted C. scheeri (n=17) were transplanted back onto the pipeline after pipeline construction was completed. Survival rates were monitored through December 2016 and compared to undisturbed C. scheeri near the pipeline ROW and those on other sites. For the plants transplanted once, no significant effect of moving the plants with soil compared to no soil (X2 = 2.9, p = 0.09), no significant effect of adding water at the time of transplant compared to not adding water (X2 = 1.2, p = 0.26), and no significant interaction among treatments (X2 = 0.06, p = 0.81) was observed. For plants transplanted twice, a significant effect of moving the plants with the soil compared to no soil (X2 = 5.0, p = 0.02) was found, while due to the random selection of plants to be transplanted twice there was too little data to adequately test other comparisons. There was no significant difference in mortality between the transplanted once (27% mortality) and the transplanted twice (31% mortality) treatments (p = 0.78), but there was a significant difference between transplanted and non-transplanted plants (2% mortality in non-transplanted plants; p < 0.05). Soil series did not appear correlated with mortality. Plants in good condition (scored 4 or 5 on scale of 0-5) at the time of transplanting had low mortality rates (16%) while plants scored 3 or lower had high mortality rates (60%) but deaths did not occur immediately after transplanting: 5 died after 8 or 9 months, 4 after 13-16 months, and 9 after 23 or 24 months. The majority of the deaths occurred after numerous months of declining in condition but six plants died suddenly. Good condition plants were more likely to flower than those in poor condition. Transplanting appears to conserve some of the C. scheeri population which would have otherwise been lost to development.