The experiment was conducted at the University of Arizona Campus Agricultural Center to evaluate the effect of soil moisture stress on photosynthesis, transpiration, diffusive resistance, temperature differential, leaf temperature, and specific leaf weight of seven sorghum Sorghum bicolor (L.) Moench cytoplasms represented by nine lines. As soil moisture stress increased, diffusive resistance and leaf temperature increased whereas photosynthesis and transpiration decreased. Temperature differential was highest under high soil moisture stress and lowest under medium soil moisture stress. Specific leaf weight was highest under medium soil moisture stress. Three lines, AKS37, AKS38, and A2Tx398, representing two different germplasms under high soil moisture stress exhibited high photosynthesis and transpiration rates, high specific leaf weights, and low diffusive resistance. Differences in photosynthesis rates under non-soil moisture stress between A1 and A2 cytoplasmic sterility systems were significant.

No description available.

Master of Science / Department of Agronomy / Ramasamy Perumal / Tesfaye Tesso / Grain mold (GM) is an important biotic constraint limiting yield and market value of sorghum grains. It results in kernel discoloration and deterioration. Such kernels have reduced seed viability, low food and feed quality. Breeding for grain mold resistance is challenging because of the complex nature of host-pathogen-environment interactions. This complex task could be made simpler by utilizing molecular markers. Utilization of marker resources may help to find genomic regions associated with grain mold resistance. In this study, three sets of field and laboratory based experiments were performed which will help in finding potential grain mold pathogens responsible for kernel deterioration in the studied environment and search for genotypes with better kernel quality and grain mold resistance. In the first part of the study, in vitro screening of 44 grain mold resistant sorghum genotypes developed and released by Texas A & M AgriLife Research. This study was aimed at identifying sources resistance to grain mold infection through laboratory screening. The result revealed that genotypes Tx3371, Tx3373, Tx3374, Tx3376, Tx3407, Tx3400, and Tx3402 were have high level of resistance and were identified as potential sources of grain mold resistance as each showed minimal fungal infection and higher grain quality traits. The second experiment was performed to optimize surface sterilization protocol for the extraction of fungal pathogens from the kernel surface (pericarp) and to study the effect of bleach percentage and time period on pathogen extraction. Seven treatments using sterilized double distilled water (0 % bleach (v/v)) and different bleach (NaOCl) concentrations (2.5, 5, 7.5, 10, 12.5 and 15 %) were used with a time interval of 2.5, 5, 7.5 and 10 min. Optimized surface sterilization in the range of 7.5 to 15 % bleach (v/v) for 7.5 to 10 min resulted least contamination and fungal genera isolation from the surface of the kernel. The third study was aimed at characterizing genotypes (sorghum association panel) for grain mold pathogen F. thapsinum and by using genome wide association (GWA) tool in order to find genomic regions associated with grain mold resistance. We studied the effect of different agronomic and panicle architecture traits on grain mold incidence and severity. Effects of grain mold on kernel quality traits were also studied. We reported two loci associated with grain mold resistance. Based on first year field screening results, 46 genotypes having grain mold ratings 1-5 (1 = < 1% panicle kernel molded; 5 = > 50% panicle kernel molded) were selected for a detailed study aimed at understanding grain mold x fungal pathogen interactions to physical and chemical kernel traits. Seed germination test, vigor index, and tetrazolium viability test were performed to study effect of grain mold infection on kernel viability and vigor. Alternaria, Fusarium thapsinum, F. verticillioides and F. proliferatum were the main fungal genera isolated from bisected kernels. Based on two year screening, SC623, SC67, SC621, SC947 and SC1494 were most resistant based on both PGMR and TGMR rating while SC370, SC833, SC1484, and SC1077 showed the most susceptible reaction and this was consistent for individual location analysis. SC309, SC213, SC833, SC971 and SC1047 are genotypes having identified loci for grain mold resistance.

Call number: LD2668 .T4 1986 L42 / Master of Science / Genetics Interdepartmental Program

LD2668 .T4 1950 C37 / Master of Science

Typescript. / Digitized by Kansas Correctional Industries

Typescript. / Digitized by Kansas Correctional Industries

Digitized by Kansas Correctional Industries

Typescript, etc. / Digitized by Kansas Correctional Industries

Typescript (photocopy). / Digitized by Kansas Correctional Industries