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The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Title from PDF of title page (University of Missouri--Columbia, viewed on October 16, 2009). Thesis advisor: Dr. Kristin D. Bilyeu. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1968. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.Sc. (Biochemistry)) -- University of Limpopo, 2011 / Soybean is one of the most cultivated legume plants in developing countries. Nodule senescence is a major limitation in producing high yields of soybean as it coincides with the pod filling stage. Delaying nodule senescence could be a way of increasing the yield of soybean therefore determination of the role of cysteine protease in soybean is of vital importance. In this study, soybean plants were grown under controlled temperature and light conditions. Leaves and root crown nodules were collected at 4, 6, 10, 12 and 16 weeks of age. In a comparative 1-dimensional SDS-PAGE analysis of soybean nodule proteomes as the plant matured, it showed differences in proteins expressed as shown by different banding patterns with less variation between the younger soybean nodule extracts (4, 6 and 10 weeks old) as compared to the older ones (12 and 16 weeks old). As determined by azocasein assay and protease zymography, the protease activity of the nodule extracts generally decreased with an increase in the age of the nodules whereas that of the leaves increased as the plants grew older. Cysteine proteases in the soybean nodule extracts readily cleaved the Z-Arg-Arg-AMC substrate with the highest activity shown in the younger nodules as compared to the older ones. In the leaf extracts, cysteine protease activity increased with age of the leaves. DCG-04, a biotinylated irreversible inhibitor, proved to be an effective label in profiling of activity of cysteine proteases in 1-dimensional and 2-dimensional systems. The labelling was inhibited specifically by cysteine protease inhibitor, E-64. In root nodules, the DCG-04 probing demonstrated that the expression of cysteine proteases is higher in early stages of development of the soybean nodules as compared to the later stages whereas in the leaves, there is higher expression of cysteine proteases in the old leaves (16 weeks). Using 2-dimensional polyacrylamide gel electrophoresis, five cysteine protease isoforms were visualised with the size ranging from approximately 25 to 30 kDa and a pI range of 4-6. In older nodules (12 and 16 weeks old) the higher pI isoforms are down-regulated with the 26 kDa and pI 4.5 protease being the predominant isoform. Affinity precipitation of the cysteine proteases yielded a strong band with the size of about 26 kDa. All assays used show that while in leaves, the expected trend of high expression of cysteine proteases in senescing leaves is observed, in soybean nodules the expression of cysteine proteases decreases with senescence. There is, therefore, no correlation between senescence and cysteine proteases in nodules. The highly expressed cysteine protease in young nodules could play a developmental or regulatory role during the early stages of development.

Doctor of Philosophy / Department of Entomology / Larry L. Buschman / C. Michael Smith / Several studies were conducted to develop soybean management options that could provide protection from the soybean stem borer, Dectes texanus LeConte. Selected soybean genotypes were screened for host plant resistance against D. texanus. Soybean plants were grown in a footprint that could be covered by a field cage. When beetles were flying in the fields they were collected and placed in the field cages to increase the insect feeding pressure on the test plants. A susceptible commercial soybean variety treated with the systemic insecticide fipronil was used as a positive antibiosis check. Both commercial soybean varieties and plant introductions (PIs) obtained from the USDA National Soybean Germplasm Collection in maturity groups (MG) VI to VIII were tested over a four-year period. Since the number of ovipositions per plant could not be controlled, the ratio of oviposition punctures (OP’s) per live larvae (OP/ Lv) was used as a novel index of potential plant antibiosis to D. texanus. Field evaluations identified PI165673 as a genotype with a very high OP/ Lv ratio - similar to that for the fipronil antibiosis control. PI165673 appears to be potential source of resistance to D. texanus. Factorial analysis indicated that soybean maturity group was not significant factor in the expression of resistance. The OP/ Lv ratio appears to be more sensitive means of identifying antibiosis than other more conventional damage indices. The use of field cages demonstrated consistent plant responses from year to year during the multi-year study. Greenhouse-grown soybean plants, including transgenic plants containing the Manduca sexta chitinase gene, were not morphologically appropriate for successful D. texanus oviposition because the greenhouse-grown plants were poorly developed and had not produced enough pith in petioles. Therefore the greenhouse results were inconclusive. Analysis of the vertical distribution of D. texanus oviposition on soybean plants revealed that D. texanus oviposition was most likely to occur on leaf petioles on the upper five nodes of the plant canopy. Histomorphological observations of plant petioles indicated that the proportion of the petiole perimeter occupied by vascular bundles might be related to D. texanus oviposition. Both foliar and seed applications of fipronil suppressed D. texanus larval damage on soybean plants. The efficacy of these treatments was sustained for long periods, even until adult beetles were present in early August. The effects of the fipronil seed-treatment and harvest date on grain yield were both significant while the interaction was not. A physiological yield loss of 8.2% and plant lodging losses of 2.9% were associated with D. texanus infestation.

Thesis (Ph.D)-University of KwaZulu-Natal, Pietermaritzburg, 2008. / Full text also available online. Scroll down for electronic link.

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