Submitted by (edna.saturno@ufrpe.br) on 2016-11-07T15:17:13Z No. of bitstreams: 1 Thiago Augusto Pereira Moraes.pdf: 1403494 bytes, checksum: 178fa823d17d452549f791d6c6584857 (MD5) / Made available in DSpace on 2016-11-07T15:17:13Z (GMT). No. of bitstreams: 1 Thiago Augusto Pereira Moraes.pdf: 1403494 bytes, checksum: 178fa823d17d452549f791d6c6584857 (MD5) Previous issue date: 2007-02-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / It was objectified to use increasing doses of pentoxifylline on the critical period of the testicular development of Wistar rats, aiming to keep high levels of AMPc in the Sertoli cells and to increase the intrinsic income of spermatogenesis and sperm production in adult rats. There were used 37 neonate Wistar rats, which were submitted to many treatments during first 21 days of life, according to experimental groups: control (n=10), 1mg/kg (n=10), 5mg/kg (n=9) and 10mg/kg (n=8) of pentoxifylline. The animal weight was obtained daily, in this period, on the control and treated groups. On the second experiment, it was used 39 adult Wistar rats, which were submitted to many treatments, between 90 and 150 days of life. in accordance with the experimental group: control (n=9), 15mg/kg (n=10), 30mg/kg (n=10) and 60mg/kg (n=10) of pentoxifylline. The animal weight was carried through daily, in this period, on the control and treated groups. At the 90 days (Exp. 1) and 150 days (Exp. 2) of the experimental period, the rats of each group were submitted to anesthesia and intracardiac perfusion. Later, the testis, epididimes and seminal vesicle were removed and weighted. The testis were fragmented (2mm) and put in perfusion solution. For studies with light microscope, the fragments were processed routinely for inclusion in plastic resin with glycol methacrylate. Histologic cuts (4 μm) were stained in blue of toluidine/borate of sodium (1%) and analyzed. On the Experiment 1, the testis weight of animals treated with 5mg/kg was higher (13%) than those observed in the animals treated with biggest dose. The seminal vesicle of the animals treated with 5mg/kg presented increase of 17% and 26% in weight in relation to the 1mg/kg and control groups, respectively. The net weight of testis had significant reduction in the group treated with 10 mg/kg when compared to the group treated with 5mg/kg. The seminiferous tubule and epithelium volumes increased in the group of 5mg/kg when compared to the control and 10mg/kg groups. The number of Sertoli cell per transversal section had significant reduction in the groups treated with 5mg/kg and 10mg/kg when compared to control and 1mg/kg groups. Spermatides rounded by transversal section had numerical reduction in the group treated with 10mg/kg when compared with animals treated with 1mg/kg. The index of Sertoli cell (ICS) significantly increased in the animals treated with 5mg/kg in comparison to the control group. On the animals that received increased doses of pentoxifylline between 90 and 150 days of life, the higer doses of pentoxifylline had decrease of testicular parenchyma on the lume of seminiferous tubules and increased the proper tunic theses tubules. On the intertubular compartment, the highest doses of this PDE’s inhibitor increased the conjutive tissue volume and decreased the lymphatic space. The volumetry of Leydig cells increased on the treated groups with the doses of 30 and 60 mg/kg de pentoxifyllina when compared with animals treated with lower doses. The number of spermatogonia A increased on the 30 and 60 mg/kg when compared to control group. In accordance with the results, the use of the pentoxifylline during the critical period of the neonatal testis development in Wistar rats was not capable to induce increase in the population of Sertoli cells, as well the pentoxifylline during 60 days in adult Wistar rats was not capable to increase the intrinsic income of spermatogenesis and sperm production. / Objetivou-se estudar a influência da pentoxifilina no desenvolvimento testicular durante o período neonatal e no processo espermatogênico de ratos Wistar adultos. Foram utilizados 37 ratos neonatos Wistar, os quais foram submetidos aos diversos tratamentos, durante os primeiros 21 dias de vida, de acordo com o grupo experimental: controle (n=10), 1mg/kg (n=10), 5mg/kg (n=9) e 10mg/kg (n=8) de pentoxifilina. Para o segundo experimento, foram utilizados 39 ratos Wistar adultos, os quais foram submetidos aos diversos tratamentos, entre 90 e 150 dias de vida, de acordo com o grupo experimental: controle (n=9), 15mg/kg (n=10), 30mg/kg (n=10) e 60mg/kg (n=10) de pentoxifilina. A pesagem dos animais foi realizada diariamente, nos grupos controle e tratados de cada experimento. Para a realização das análises histológicas e histométricas, os ratos de cada experimento foram submetidos à heparinização e anestesia e, posteriormente, submetidos à perfusão intracardíaca com solução fisiológica de NaCl a 0,9%, acrescida de heparina sódica e nitroprussiato. Após a lavagem do sistema vascular, os animais foram perfundidos com solução fixadora de glutaraldeído a 4%, em tampão fosfato de sódio, pH 7,2 e 0,01M. Posteriormente, os testículos, epidídimos e vesícula seminal foram removidos e pesados. Os testículos foram seccionados em fragmentos de 2mm e refixados na mesma solução de perfusão. Para os estudos ao microscópio de luz, os fragmentos foram processados rotineiramente para inclusão em resina plástica à base de glicol metacrilato. Cortes histológicos de 4 μm de espessura foram corados em azul de toluidina/borato de sódio a 1% e analisados. Em relação ao primeiro experimento, o peso testicular dos animais tratados com 5mg/kg foi maior 13,3% do que o observado nos animais tratados com a maior dose. O número de células de Sertoli por secção transversal sofreu redução significativa nos grupos tratados com 5mg/kg e 10mg/kg em relação aos grupos controle e tratado com 1mg/kg. O índice de célula de Sertoli (ICS) aumentou significativamente nos animais tratados com 5mg/kg em comparação ao grupo controle. Em se tratando do segundo experimento, as maiores doses de pentoxifilina promoveram redução no volume do parênquima testicular alocado no lume dos túbulos seminíferos e aumentaram a túnica própria destes túbulos. A volumetria das células de Leydig aumentou nos grupos tratados com as doses de 30 e 60mg/kg de pentoxifilina quando comparada com os animais tratados com a menor dose. Conclui-se que a utilização da pentoxifilina durante o período crítico do desenvolvimento testicular neonatal em ratos Wistar não foi capaz de induzir aumento na população das células de Sertoli, assim como a utilização da pentoxifilina durante 60 dias em ratos Wistar adultos não foi capaz de aumentar o rendimento intrínseco da espermatogênese e produção espermática.