A dissertation submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of requirements for the degree of Master of Science. Johannesburg, 2016. / Stable isotope analyses have been used to infer diets of organisms, define trophic partitioning, and infer geographic origins of species. It has further been applied to forensic ecology to infer the origins of deceased humans and illegally traded animal (elephant ivory and rhinoceros horn) and plant (cycads, coca, and cannabis) material. However, no research has focused on the isotope analysis of avian material in forensic ecology. African grey parrots Psittacus erithacus are one of the most traded species in the world, with the trade often being illegal, and the origins of confiscated or deceased specimens being unknown. The aim of this study was to determine if stable isotopes (δ 13C, δ 15N, and δ 2H) in African grey parrot feathers could be used to determine the wild or captive origins of birds. African grey parrot feathers (primary, body, and tail) differed isotopically so standardising isotope values of African grey parrot feathers to a single feather type was recommended, to maintain consistent sampling and allow for comparisons to be drawn between different feather types. African grey parrot feathers from unknown origins can be identified as wild or captive using δ 13C and δ 2H values, but not δ 15N values. Known wild and captive feathers possibly differ isotopically from one another because of dietary and location differences. Wild African grey parrots inhabit and feed in isotopically depleted C3 forests compared to captive African grey parrots which are usually fed C4 based foods with more positive isotope values. Wild African grey parrot δ 2H isotope values were the most negative in the central region of their native distribution. The ability to differentiate wild from captive African grey parrots, as well as infer basic origins (East from West Africa) may improve the monitoring of the illegal trade as well as help in tracing illegally traded parrots. / LG2017

Thesis (MTech (Nature Conservation))--Cape Peninsula University of Technology, 2017. / Stable isotope analysis (SIA) of whiskers has been used to identify temporal feeding habits, intra-population diet variation, as well as individual dietary specialisation of marine and terrestrial carnivores. However, the potential of the method to disclose such dietary information for large wild felids has been little explored. The accurate interpretation of stable isotope ratios along serially sampled whiskers is hampered by lack of information on species-specific whisker growth rates, whisker growth patterns and whisker-diet trophic discrimination factors (TDFs). Whisker growth rate and growth pattern informs on the time period encapsulated in the analysed segment of a whisker, while whisker-diet TDFs are required to make correct deductions of the prey species consumed by a predator. The aim of this study was to develop and evaluate the technique of using stable carbon and nitrogen isotope analysis of felid whiskers to quantify the diet of wild felids and in particular, to identify diet variation among individuals. To achieve this, lion Panthera leo and leopard Panthera pardus whisker growth rate and growth pattern, and lion whisker-diet TDFs were measured, using captive individuals held at the National Zoological Gardens, Pretoria. The viability and applicability of the technique was then explored on six free-ranging leopards in Phinda Private Game Reserve (hereafter Phinda), northern KwaZulu-Natal (KZN) whose diets have been intensively studied using traditional methods. Whisker growth rates and growth patterns were measured for four lions (three sub-adult females and one adult male) and an adult male leopard over 185 days using giraffe Giraffa camelopardalis meat as an endogenous biomarker to consecutively mark whiskers as they grew. The 13C-depleted, C3-derived giraffe meat with its characteristic isotopic signature could be discerned from the 13C-enriched diet of C4 grain-fed beef and chicken the felids were sustained on. Two whiskers were removed from each felid at the beginning of the experiment, and felids were fed the giraffe meat at four predetermined periods to mark the whiskers replacing the removed ones. The periods with low δ13C values, identified following serial sectioning of the regrown whiskers at 1 mm intervals (and stable isotope analysis of these sections), were then correlated to specific giraffe meat feeding bouts and hence growth periods. Knowledge of the duration between giraffe meat feeding bouts enabled the calculation of whisker growth rate and determination of growth pattern. δ13C and δ15N whisker-diet TDFs were estimated for five lions whose diet remained consistent over multiple years. Whiskers removed from four lions at the beginning of the whisker growth experiment, a whisker removed from a female lion as part of a pilot study a year before the experiment and the diet (chicken and beef) samples collected during the experiment were analysed for their isotopic ratios. These were used to calculate isotopic differences between lion whiskers and diet.

Thesis (M.S. - Hydrology and Water Resources)--University of Arizona. / Includes bibliographical references (leaves 118-121).

Thesis (Ph.D.)--University of Central Florida, 2008. / Adviser: Graham A. J. Worthy. Includes bibliographical references.

Stable isotope analyses are powerful tools for reconstructing ancient ecologies and ecosystems, as they are independent of morphology and directly reflect dietary ecology. The application of stable isotope analyses, however, is not without limitations, as determination of food web dynamics using these methods often relies on poorly tested assumptions. The guiding thread of this thesis is the testing of foundational cornerstones on which these methods rely, in order to validate the suitability of applying these techniques to different mammalian clades, and to more reliably and confidently interpret the isotopic signals preserved in extinct organisms. The first chapter of this thesis tests the validity of an important assumption behind the interpretation of stable carbon isotope analyses for understanding diet in terrestrial mammalian herbivores: if, as assumed for almost two decades, mammalian bioapatite δ¹³C is enriched by 14‰ relative to dietary δ¹³C. By analyzing new isotopic data from a never before assessed herbivorous group spanning a broad range of body masses—sloths (Xenarthra, Mammalia)— and other mammals with experimentally controlled or observationally known diets, I discovered considerable variation in diet–bioapatite δ¹³C enrichment among mammals. Statistical tests (ordinary least squares, quantile, robust regressions, Akaike information criterion model tests) documented independence from phylogeny, and a previously unrecognized strong and significant correlation of δ¹³C enrichment with body mass for all mammalian herbivores. A single-factor body mass model outperformed all other single-factor or more complex combinatorial models evaluated, including for physiological variables (metabolic rate and body temperature proxies), and indicated that body mass alone predicts δ¹³C enrichment. These analyses, spanning more than 5 orders of magnitude of body sizes, yield a size-dependent prediction of isotopic enrichment across Mammalia and for distinct digestive physiologies, permitting reconstruction of foregut versus hindgut fermentation physiologies for fossils and refined mean annual paleoprecipitation estimates based on δ¹³C of mammalian bioapatite. Second, I sought to evaluate the existing paradigm governing identification of closed canopy rainforests in the fossil record using mammalian δ¹³C data: the presence of mammals with dietary δ¹³C <-31‰, which has only been observed in closed canopy rainforests in Equatorial Africa, the only other tropical ecosystem sampled extensively. This chapter provides a characterization of δ¹³Cbioapatite, δ¹³Chair and δ¹⁵Nhair of a modern mammalian community in western Amazonia, in Peru, to test if the isotopic structure of mammals in this Neotropical ecosystem is similar to those in African tropical rainforests. The results indicate that despite their marked geographical and taxonomic differences, median δ¹³Cdiet values from closed canopy rainforests in Amazonia (-27.4‰) and equatorial Africa (-26.9‰) are not significantly different. Amazonian mammals, however, seem to exploit a narrower spectrum of dietary resources than equatorial African mammals, as depicted by the absence of highly negative δ¹³Cdiet values previously proposed as indicative of rainforests (<-31‰). I hypothesize that differential effects of late Pleistocene extinction may be responsible for the ecological disparities among the two rainforests, by significantly reducing evolutionary time and dietary breadth reflected in the modern Amazonian mammalian community. Finally, the third chapter of this dissertation evaluates assumptions behind δ¹⁵N amino acid compound specific analyses in order to test the controversial hypothesis of carnivory and consumption of proteins of animal origin in fossil sloths. This analytical technique relies on three main assumptions. First, that the offset between the δ¹⁵N of glutamic acid (δ¹⁵NGlx) and phenylalanine (d15NPhe) in the organism under study will increase with increasing trophic level. Second, that the offset between δ¹⁵NGlx and δ¹⁵NPhe at the base of the food chain is relatively constant and has a value of -8.4‰ for C3 ecosystems. Third, that the trophic discrimination factor in all ecosystems (the difference in δ¹⁵NGlx relative to δ¹⁵NPhe with increasing trophic level) is 7.6‰. The results of my experiments conducted on extant xenarthrans (sloths and anteaters) with controlled diets document that only the first assumption holds true. Rather than relying on an equation with constants introducing uncertainties and that are not applicable to organisms feeding on a combination of items of different origin (e.g., C3 + C4 plants), δ¹⁵NGlx and δ¹⁵NPhe values by themselves can accurately reconstruct the trophic position of organisms. Indeed, the results on δ¹⁵NGlx and δ¹⁵NPhe herein obtained for five xenarthran species in controlled feeding experiments, combined with mammalian data available from the literature, show strong and significant correlations between these two AAs and with trophic positions. Both the TP equation and the regression analyses of δ¹⁵NGlx and δ¹⁵NPhe suggest that the Pleistocene fossil ground sloths 𝘔𝘺𝘭𝘰𝘥𝘰𝘯 𝘥𝘢𝘳𝘸𝘪𝘯𝘪𝘪 and 𝘕𝘰𝘵𝘩𝘳𝘰𝘵𝘩𝘦𝘳𝘪𝘰𝘱𝘴 𝘴𝘩𝘢𝘴𝘵𝘦𝘯𝘴𝘪𝘴 were not pure herbivores as commonly presumed, but rather that they were both mixed feeders/omnivores, incorporating items of animal origin in their diets.

No description available.

Thesis (Ph.D. (Zoology & Entomology)) - Rhodes University, 2008.

Thesis (M.S.)--Southern Methodist University, 2007. / Includes bibliographical references.

There are broad differences in regional oceanography and primary production around the South African coast, which we might expect to give rise to major differences in trophic pathways. δ⁻¹³C and δ⁻¹⁵N isotopic ratios of suspended particulate matter (SPM), mussels, various intertidal consumers and common macroalgae along the South African coastline were explored using stable isotope analysis to investigate biogeographic and temporal variability of isotopic signatures of marine intertidal consumers and their food sources around the coast of South Africa, with a focus on evaluating the dependence of intertidal mussels on phytoplankton and macroalgal-derived organic carbon. Isotopic equilibration rates of four mussel tissues were determined through laboratory feeding experiments, which established that adductor tissue had the slowest isotopic turnover rate, and was subsequently used as an indication of overall mussel diet. Biogeographic, temporal and nearshore/offshore trends of isotopic ratios of SPM were investigated along 10km transects perpendicular to the coast and SPM exhibited overall trends of carbon depletion when moving from west to east along the coastline and from nearshore to offshore water, in both cases suggesting a shift from macrophyte detritus to a phytoplankton signature. δ⁻¹³C signatures of SPM also revealed temporal and biogeographic variation that had strong ties to local oceanography, being closely correlated to regional hydrographic features and tidal influences. Mixing models indicated filter feeders demonstrated over 50% dependence on nearshore SPM for organic carbon and it was possible to categorize them into geographic groups based on their carbon and nitrogen signatures, suggesting biogeographic shifts in resources. Biogeographic shifts in diet were also seen in some grazers. Difficulties in relating macroalgae to mussel diet led to investigations into the isotopic changes associated with macroalgal decomposition. Variation in photosynthetic fractionation, leaching and microbial mineralization are believed to have resulted from species-specific patterns of degradation. Although the strong links between carbon signatures and local oceanography indicate that stable isotope analysis is a powerful tool for the study of water mixing and coastal hydrography in relation to food-web analyses, substantial variation in fractionation of primary consumers, along with different periods of time integration between consumers and their food sources must be considered in future studies, to resolve trophic links in marine food webs successfully.

Two pocket sandy beaches, Eastern Cape, South Africa, were investigated, to determine whether these beaches were subsidised by an adjacent mangrove estuary, by using stable light isotope (δ 13C and δ 15N) analyses. The trophodynamics and macrofaunal food webs of these two beaches, situated between the Mgazi and Mgazana estuaries (in a warm-temperate/subtropical transition zone,), were described. Two to 2.5 trophic levels were identified for the macrobenthic community, with suspension feeders and omnivorous scavengers as the primary consumers, and carnivores as the secondary consumers. Mangrove material and terrigenous inputs were not driving the sandy beach food webs. Instead, marine allochthonous inputs (carrion, macroalgae), possibly phytoplankton, sediment organic matter, and resident macroinfauna were the dominant food sources. Cattle dung could have been the only important terrigenous food source utilised by the beach benthos. The macroinfauna displayed generalist/omnivorous feeding strategies, but within the limits of predominantly marine food sources. There was evidence that carnivores actively preyed on resident beach fauna. Omnivory and intraguild feeding might also be important biological processes in these communities. Seasonal and spatial variability in stable isotope composition of the fauna was observed, but few patterns were evident. There was a general trend of more enriched δ 15N and δ 13C composition of animal tissues in summer as opposed to winter. This was accompanied by a general decrease in C:N ratios in summer. It was hypothesised that these isotopic and biochemical changes were in response to increased food availability during summer. Although mangrove material appeared not to play an important role in the nutrition of these sandy beach communities, it was suggested that a high retention time of particles in the bay could enhance bacterial decay of particulate mangrove material, which could then act as fine, bacterial-enriched particulate food to the macrobenthos. This remains to be tested.