Indiana University-Purdue University Indianapolis (IUPUI) / Despite growing recognition as a significant natural resource, methods for accurately estimating urban tree canopy cover extent and change over time are not well-established. This study evaluates new methods and data sources for mapping urban tree canopy cover, assessing the potential for increased accuracy by integrating high-resolution satellite imagery and 3D imagery derived from LIDAR and stereoscopic sensors. The results of urban tree canopy classifications derived from imagery, 3D data, and vegetation index data are compared across multiple urban land use types in the City of Indianapolis, Indiana. Results indicate that incorporation of 3D data and vegetation index data with high resolution satellite imagery does not significantly improve overall classification accuracy. Overall classification accuracies range from 88.34% to 89.66%, with resulting overall Kappa statistics ranging from 75.08% to 78.03%, respectively. Statistically significant differences in accuracy occurred only when high resolution satellite imagery was not included in the classification treatment and only the vegetation index data or 3D data were evaluated. Overall classification accuracy for these treatment methods were 78.33% for both treatments, with resulting overall Kappa statistics of 51.36% and 52.59%.