This research develops a procedure to determine the applied stresses in rails using the acoustoelastic effect of ultrasonic waves. Acoustoelasticity is defined as the stress dependency of ultrasonic wave speed or wave polarization. Analytical models are developed that predict the acoustoelastic effect for longitudinal waves, shear waves, Lamb waves, and Rayleigh waves. Using a programming tool, a numerical simulation of the models is generated to obtain the stress dependent curves of wave velocity and polarization of the various ultrasonic waves propagating in rail steel. A comparison of the sensitivity of the acoustoelastic effect is made to determine the feasibility of ultrasonic waves for further study. Rayleigh waves are found to be most sensitive to stress change. Rayleigh waves are generated using ultrasonic transducer and detected using a laser Doppler vibrometer (LDV). The LDV measures the in-plane and out-of-plane velocities. Polarization is defined as the ratio of in-plane and out-of-plane displacements. Initially, polarization is determined for the specimen in unstressed condition. Thereafter, the rail specimen is stressed in a compression testing machine, the experiment repeated, and the polarization determined. Thus, Rayleigh wave polarization is obtained as a function of applied stress. Finally, the change in polarization obtained experimentally is compared with the analytical model.

This research develops a procedure to determine the applied stresses in rails using the acoustoelastic effect of ultrasonic waves. Acoustoelasticity is defined as the stress dependency of ultrasonic wave speed or wave polarization. Analytical models are developed that predict the acoustoelastic effect for longitudinal waves, shear waves, Lamb waves, and Rayleigh waves. Using a programming tool, a numerical simulation of the models is generated to obtain the stress dependent curves of wave velocity and polarization of the various ultrasonic waves propagating in rail steel. A comparison of the sensitivity of the acoustoelastic effect is made to determine the feasibility of ultrasonic waves for further study. Rayleigh waves are found to be most sensitive to stress change. Rayleigh waves are generated using ultrasonic transducer and detected using a laser Doppler vibrometer (LDV). The LDV measures the in-plane and out-of-plane velocities. Polarization is defined as the ratio of in-plane and out-of-plane displacements. Initially, polarization is determined for the specimen in unstressed condition. Thereafter, the rail specimen is stressed in a compression testing machine, the experiment repeated, and the polarization determined. Thus, Rayleigh wave polarization is obtained as a function of applied stress. Finally, the change in polarization obtained experimentally is compared with the analytical model.

This research develops a procedure to determine the applied stresses in rails using the acoustoelastic effect of ultrasonic waves. Acoustoelasticity is defined as the stress dependency of ultrasonic wave speed or wave polarization. Analytical models are developed that predict the acoustoelastic effect for longitudinal waves, shear waves, Lamb waves, and Rayleigh waves. Using a programming tool, a numerical simulation of the models is generated to obtain the stress dependent curves of wave velocity and polarization of the various ultrasonic waves propagating in rail steel. A comparison of the sensitivity of the acoustoelastic effect is made to determine the feasibility of ultrasonic waves for further study. Rayleigh waves are found to be most sensitive to stress change. Rayleigh waves are generated using ultrasonic transducer and detected using a laser Doppler vibrometer (LDV). The LDV measures the in-plane and out-of-plane velocities. Polarization is defined as the ratio of in-plane and out-of-plane displacements. Initially, polarization is determined for the specimen in unstressed condition. Thereafter, the rail specimen is stressed in a compression testing machine, the experiment repeated, and the polarization determined. Thus, Rayleigh wave polarization is obtained as a function of applied stress. Finally, the change in polarization obtained experimentally is compared with the analytical model.

This research develops a procedure to determine the applied stresses in rails using the acoustoelastic effect of ultrasonic waves. Acoustoelasticity is defined as the stress dependency of ultrasonic wave speed or wave polarization. Analytical models are developed that predict the acoustoelastic effect for longitudinal waves, shear waves, Lamb waves, and Rayleigh waves. Using a programming tool, a numerical simulation of the models is generated to obtain the stress dependent curves of wave velocity and polarization of the various ultrasonic waves propagating in rail steel. A comparison of the sensitivity of the acoustoelastic effect is made to determine the feasibility of ultrasonic waves for further study. Rayleigh waves are found to be most sensitive to stress change. Rayleigh waves are generated using ultrasonic transducer and detected using a laser Doppler vibrometer (LDV). The LDV measures the in-plane and out-of-plane velocities. Polarization is defined as the ratio of in-plane and out-of-plane displacements. Initially, polarization is determined for the specimen in unstressed condition. Thereafter, the rail specimen is stressed in a compression testing machine, the experiment repeated, and the polarization determined. Thus, Rayleigh wave polarization is obtained as a function of applied stress. Finally, the change in polarization obtained experimentally is compared with the analytical model.

Implementation of the ultrasonic second harmonic generation has typically been restricted to simple setups such as through-transmission or Rayleigh surface waves. Recent research has evaluated the second harmonic generation in P- and SV- waves reflected from a stress-free surface to enable the single-sided interrogation of a specimen. This research considers the second harmonic generation in an aluminum specimen, which is analytically evaluated using an approach based on the perturbation method. Here, the model is chosen to mimic an experimental setup where a longitudinal wave is generated at an oblique angle and the reflected wave is detected using a set of wedge transducers. Due to mode conversion at the interface of the wedge and the specimen, it is necessary to evaluate longitudinal and shear waves, determining all second harmonic waves generated in the bulk and at the stressfree boundary. The theoretically developed model is then implemented in a commercial finite element code, COMSOL, using increasing fundamental wave amplitudes for different values of third order elastic constants. The results of this computational model verify the analytical approach and the proposed measurement setup, taking into account assumptions and approximations of the solution procedure. Furthermore, the computational model is used to draw important conclusions relevant to the experimental setup, including the need to avoid evolving surface waves and interactions with diffracted waves. These numerical results are used to develop a recommendation for the measurement position and incident angle. Finally, the nonlinearity of two different aluminum specimens is measured with the suggested measurement setup and the results confirm the feasibility of the single-sided determination of the acoustic nonlinearity using reflected bulk waves.

The studies described in this dissertation were performed with the goal of understandingthe function of antioxidant compounds delivered in vivo to rodents and the implication of theresults towards oxidative stress (OS)-related neurodegenerative disorders with particularemphasis on Alzheimer's disease (AD). OS has been implicated in AD and is characterized byextensive oxidative damage to protein, lipids and DNA. A major thrust of this dissertation workwas to gain insight into antioxidant properties of compounds used in the following studies andtheir efficacy as potential therapeutics for treatment of OS-related disorders.D609, a glutathione (GSH) mimetic is known to trap OH. Radicals, scavenge H2O2 andreduce the A?? (1-42)-induced OS and cytotoxicity in neurons. The present dissertation studyshowed in vivo protective effect of D609 in synaptosomes and mitochondria isolated fromgerbils against OS mediated by Fe2+/H2O2, AAPH, and A?? (1-42). Upon intraperitonial (i.p.)injection of gerbils, D609 showed protection of subsequently isolated brain moieties against OS.In vivo administration of D609 also modulates brain GSH levels and increases the activity of keyGSH-related enzymes, thereby likely provides a protection against OS.Adriamycin (ADR), a quinone-containing chemotherapeutic, is known to produce ROS inheart. Patients under treatment with ADR often show persistent changes in cognitive function(effect called as chemobrain by patients). Upon i.p. injection, ADR causes OS, increasesexpression of multidrug resistant protein-1 (MRP-1) in brain and alters GSH levels and itsrelated enzyme activities. ??-Glutamyl cysteinyl ethyl ester (GCEE) is known to increase GSHlevels in brain, in vivo. Research reported in this dissertation shows that in vivo GCEE reversesthe ADR-mediated OS in mice brain.N-acetylcysteine (NAC), a GSH precursor provides the limiting substrate cysteine inGSH synthesis. Previously, our laboratory showed increased GSH levels post i.p. injection ofNAC and reduces OS in synaptosomes treated with acrolein. The present study showed thatNAC given in drinking water to APP/PS-1 mice, a model of AD can significantly reduce OS.These results provide a potential therapeutic intervention by antioxidants that can modulateGSH in OS-mediated neurodegenerative disorders.

Corrosion and fatigue have been dominant degradation mechanisms in offshore structures, with the combination of the two, known as corrosion fatigue, having amplified effects in structures in the harsh marine environments. Newer types of structure are now being developed for use in highly dynamic, harsh marine environments, particularly for renewable energy applications. However, they have significantly different structural details and design requirements compared to oil and gas structures, due to the magnitude and frequency of operational and environmental loadings acting on the support structures. Therefore, the extent of corrosion assisted fatigue crack growth in these structures needs to be better understood. In this research, fatigue crack growth in S355J2+N steel used for offshore wind monopile fabrications was investigated in air and free corrosion conditions. Tests were conducted on parent, HAZ and weld materials at cyclic load frequencies similar to what is experienced by offshore wind monopile support structures. The seawater used for testing was prepared according to ASTM D1141 specifications and was circulated past the specimens through a purpose designed and built corrosion rig at a rate of 3 l/min, at a temperature of 8-100C and at a pH of 7.78-8.1. A new crack propagation method accompanied by constant amplitude loading was used. Crack growth rates in parent, HAZ and weld materials were significantly accelerated under free corrosion conditions, at all the stress ratios used compared to in air environment. However, in free corrosion conditions, crack growth rates in the parent, HAZ and weld materials were similar, particularly at a lower stress ratio. The results are explained with respect to the interaction of the loading condition, environment and the rate of material removal by corrosion in the weldments. A new model was developed to account for mean stress effects on crack growth rates in air and in seawater, and was found to correlate well with experimental data as well as with the other mean stress models tested.

Thesis (Ph. D.)--University of Texas at Austin, 2008. / Vita. Includes bibliographical references.

Oxidative stress is imbalance between oxidant and antioxidant levels in living systems. Human cells are protected from reactive oxygen species by endogenous enzymatic antioxidants. Most of these compounds require particular redox metals in their structures as cofactors to allow them to scavenge the free radicals such as Cu, Zn-SOD, Mn-SOD and catalase (Fe). The aim of this study was to quantify these metals in human cells to evaluate their effectiveness as novel biomarkers for measuring oxidative stress. The metals (Zn, Cu, Fe, Mn) were measured in vitro in skeletal muscle cells (C2C12) which were incubated under hypoxia/hyperoxia conditions generated by varying oxygen level from 1%-60% for 24 and 48 hours. Two methods were used to perform the analysis. ICP-MS was applied to liquid samples to quantify Zn, Cu, Fe and Mn in cell populations. And LA-ICP-MS was employed to solid samples to measure their intensity in individual cells. The data acquired from both techniques are positively correlated confirming the reliability of the two approaches. All elements of interest were successfully measured except Mn which was not detected in single cells using LA-ICP-MS due to the limit of detection. Interestingly, the results showed that their concentration increased dramatically in cells grown at 25%-60% O2, the most significant increase was in Cu at 60%O2. None showed any increase at 5%-15% O2 indicating normoxia states. At 1%O2, all elements except Fe showed a significant increase and the most remarkable growth was in Mn. More interestingly, increasing incubation to 48 hours for liquid samples had differing effects on the elements. Zn and Cu concentrations were unaffected by increasing incubation time except at 60%O2 where they showed further growth. In contrast, Mn concentration grew sharply over oxygen levels of 30%-50% with no further effect at 1%, while Fe concentration decreased at 1%O2 and grew steadily over oxygen levels of 5%-60%. It can be concluded that all four elements were significantly affected by stress conditions applied to cells, but at different rates. Importantly, a novel analytical method was introduced in this current study since there have been no previous reported investigations measuring changes in concentration of redox-active elements in human cells subjected to different controlled oxidative stress conditions in vitro.

Aims: Oxidative stress and inflammation contribute to atherogenesis. Rac1 GTPase regulates pro-oxidant NADPH oxidase activity, reactive oxygen species (ROS) formation, actin cytoskeleton organization and monocyte adhesion. We investigated the vascular effects of pharmacological inhibition of Rac1 GTPase in mice. Methods and Results: We treated wild-type and apolipoprotein E-deficient (ApoE−/−) mice with Clostridium sordellii lethal toxin (LT), a Rac1 inhibitor, and assessed vascular oxidative stress, expression and activity of involved proteins, endothelial function, macrophage infiltration, and atherosclerosis development. LT-treated wild-type mice displayed decreased vascular NADPH oxidase activity and ROS production. Therapeutic LT doses had no impact on behavior, food intake, body weight, heart rate, blood pressure, vascular and myocardial function, differential blood count, and vascular permeability. ApoE−/− mice were fed a cholesterol-rich diet and were treated with LT or vehicle. LT treatment led to decreased aortic Rac1 GTPase activity, NADPH oxidase activity and ROS production, but had no impact on expression and membrane translocation of NADPH oxidase subunits and RhoA GTPase activity. LT-treated mice showed improved aortic endothelium-dependent vasodilation, attenuated atherosclerotic lesion formation and reduced macrophage infiltration of atherosclerotic plaques. Concomitant treatment of cholesterol-fed ApoE−/− mice with LT, the specific synthetic Rac1 inhibitor NSC 23766 or simvastatin comparably reduced aortic Rac1 activity, NADPH oxidase activity, oxidative stress, endothelial dysfunction, atherosclerosis development, and macrophage infiltration. Conclusions: These findings identify an important role of the small GTPase Rac1 in atherogenesis and provide a potential target for anti-atherosclerotic therapy.