Orientadora: Profa. Dra. Natalia Pirani Ghilardi-Lopes / Dissertação (mestrado) - Universidade Federal do ABC, Programa de Pós-Graduação em Evolução e Diversidade, São Bernardo do Campo, 2018. / Apesar da importância do monitoramento das unidades de conservação (UCs) marinhas, problemas logísticos e orçamentários dificultam sua realização e, consequentemente, a gestão efetiva dessas áreas. Por isso, é necessária a busca por alternativas de baixo custo que possam auxiliar nessas questões. A abordagem por biótopos associada à ciência cidadã mostra-se adequada para o monitoramento de comunidades marinhas bentônicas por possibilitar extensa amostragem em curto período de tempo e com baixo custo, além de integrar cidadãos ativamente em produções de conhecimento. Essa pode ser uma ferramenta interessante para o monitoramento de UCs marinhas; porém, é importante que a elaboração do protocolo de monitoramento e sua validação sejam realizadas considerando-se as recomendações da literatura. Nesse sentido, este trabalho se propôs a (1) avaliar o status dos protocolos de ciência cidadã para bentos marinho e costeiro no mundo e (2) a propor, aplicar e validar o uso da ciência cidadã associada à abordagem por biótopos como uma ferramenta para o monitoramento de comunidades bentônicas em UCs marinhas. Para isso, realizamos o levantamento e comparação da estrutura de protocolos de ciência cidadã para bentos costeiro e marinho, incluindo o protocolo proposto no presente estudo. Apesar da maioria dos projetos encontrados apresentarem várias das características recomendadas pela literatura, seus principais interesses são a coleta de dados e os resultados oriundos destes, ficando a educação dos CCs, sua inclusão no processo de desenvolvimento do protocolo e sua avaliação em segundo plano. Sugerimos que os protocolos considerem a educação científica e ambiental dos CCs como um objetivo a ser atingido, e nosso protocolo leva tais ideias em consideração. Para embasar a elaboração do nosso protocolo, realizamos um levantamento prévio dos biótopos de um trecho do infralitoral consolidado da Ilha das Palmas (Estação Ecológica Tupinambás, Ubatuba/SP). Nesse levantamento, foram identificados 20 biótopos e três animais vágeis associados em apenas um mergulho. Também foram detectados organismos invasores e a dominância de tapetes na área de estudo. Isso é um demonstrativo de que a caracterização de comunidades marinhas bentônicas utilizando o método visual por biótopos pode agilizar a identificação de possíveis impactos negativos e a realização de planos de prevenção ou mitigação dos mesmos. Cientistas cidadãos (CCs) voluntários foram capacitados no uso do protocolo, que foi dividido em duas etapas: (1) a coleta de dados, que consistiu de registros fotográficos padronizados do costão rochoso; e (2) o processamento das fotografias, que consistiu da identificação (ID) dos biótopos nas fotos em um curso presencial. As fotos obtidas durante a Etapa 1 estão disponíveis na plataforma Zooniverse, através do projeto Monitore Tupinambás (https://www.zooniverse.org/projects/larissakawabe/monitore-tupinambas), no qual qualquer voluntário pode contribuir com as análises, seguindo o tutorial disponível. A avaliação educacional da pesquisa, realizada através de questionários pré e pós-atividade, mostrou principalmente que houve mudanças de percepção dos CCs em relação ao ambiente estudado. Para a validação dos dados da Etapa 2, comparamos as IDs dos biótopos realizadas pelas pesquisadoras (grupo controle) e CCs (grupo experimental), analisando a precisão e acurácia das respostas. Apesar das limitações inerentes do método utilizado (ID por foto) e das necessidades de modificação do protocolo identificadas por CCs e pesquisadoras; os resultados de acurácia e precisão foram comparáveis aos de outros trabalhos similares, demonstrando que esse protocolo, o primeiro a ser desenvolvido com foco em bentos marinho no Brasil, é uma ferramenta promissora para o monitoramento participativo e de longo prazo das UCs marinhas do país, auxiliando na gestão dessas áreas e na educação ambiental e científica dos cidadãos participantes. / Despite the importance of monitoring the marine protected areas (MPAs), logistical and budgetary problems make it difficult to carry out and, consequently, affect the effective management of these areas. Therefore, it is necessary to search for low-cost alternatives that could help with these issues. The biotope approach associated with citizen science could be interesting for the monitoring of marine benthic communities because it allows extensive sampling in a relatively short period of time and at low cost. At the same time, citizens are integrated as active contributors in the knowledge production. This can be an interesting tool for the MPA monitoring; however, it is important that the elaboration of the monitoring protocol and its validation consider the literature recommendations. In this sense, this work aimed to (1) evaluate the status of citizen science protocols for marine and coastal benthos in the world and (2) to propose, apply and validate the use of citizen science associated with the biotope approach as a tool for monitoring benthic communities in MPAs. For this, we conducted a survey and comparison of the structure of citizen science protocols for coastal and marine benthos, including the protocol proposed in the present study. Although most of the projects found present several of the characteristics recommended by the literature, their main interests are the data collection and their results, being the education of citizen scientists, their inclusion in the protocol development process and their evaluation in the background. We suggest that the protocols consider the scientific and environmental education of citizen scientists as a goal to be achieved, and our protocol takes such ideas into account. To support the elaboration of our protocol, we carried out a preliminary biotopes survey of a consolidated subtidal section of the Ilha das Palmas (Tupinambás Ecological Station, Ubatuba/SP). In this survey, 20 biotopes and three associated animals were identified in only one dive. Invasive organisms and carpet dominance were also detected in the study area. This is a demonstration that the benthic marine communities characterization using the biotope visual method can speed up the identification of possible negative impacts and the implementation of prevention or mitigation plans. Volunteer citizen scientists were trained in the use of the protocol, which was divided into two steps: (1) data collection, which consisted of standardized photographic records of the rocky reef; and (2) the processing of the photographs, which consisted of identification (ID) of the biotopes in the photos in a classroom course. The photos obtained during Stage 1 are available on the Zooniverse platform through the Monitore Tupinambás project (https://www.zooniverse.org/projects/larissakawabe/monitore-tupinambas), in which any volunteer can contribute to the analyzes, following the tutorial available. The educational evaluation of the research, performed through pre and post-activity questionnaires, showed mainly that there were changes in perception of citizen scientists in relation to the studied environment. For the data validation of Stage 2, we compared the biotope IDs performed by the researchers (control group) and citizen scientists (experimental group), analyzing their accuracy and precision. Despite the inherent limitations of the method used (ID per photo) and the identification by citizen scientists and researchers of protocol modification needs; the results of accuracy and precision were comparable to those of other similar studies, demonstrating that this protocol, the first developed with a focus on marine benthos in Brazil, is a promising tool for the participatory and long-term monitoring of the country's MPA, assisting in the management of these areas and in the environmental and scientific education of the citizens scientists.

The Sulaiy Formation, which is the oldest unit in the Lower Cretaceous succession, is conformably overlain by the Yamama Formation and it is a challenge to identify the precise age of the two formations using foraminifera and other microfossil assemblages. In the eastern side of Saudi Arabia, the Sulaiy Formation and the base of Yamama Formation are poorly studied. The main objectives of this study is to enhance the understanding of the Sulaiy Formation sequence stratigraphical correlation, regional lateral variations and palaeoenvironmental investigation. Lithological and semi-quantitative micropalaeontological analysis of 1277 thin sections taken from core samples from nine cored wells providing a geographically representative distribution from the Saudi Arabian Gulf. These cores intersected the base of the Yamama Formation and the Sulaiy Formation in the total thickness of cored wells of 843.23 meters (2766.5 feet). On the evidence provided by the foraminifera, the Sulaiy Formation is considered to represent the Berriasian to the lowermost Valanginian. The investigation of the micropalaeontology has provided considerable insights into the biocomponents of Sulaiy and the base of Yamama formations in order to identify their biofacies. These microfossils include rotalid foraminifera, miliolid foraminifera, agglutinated foraminifera, calcareous algae, calcispheres, stromatoporoids, sponge spicules, problematica (e.g. Lithocodium aggregatum), molluscs, corals, echinoderms and ostracods. Systematics of planktic and benthic foraminifera is accomplished using the foraminiferal classification by Loeblich and Tappan (1988) as the main source. The assemblage contains foraminifera that recorded for the first time in the Sulaiy Formation. Other microfossils were identified and recorded to help in the identification of the sedimentary environments. The investigation of the micropalaeontology and the lithofacies analysis have provided evidence the identification of the various lithofacies. About twenty four microfacies were identified on the basis of their bio−component and non-skeletal grains. The lithofacies and the bio−component results have provided the evidence of the sedimentary palaeoenvironmental model namely the Arabian Rimmed Carbonate Platform. This palaeoenvironmental depositional model is characterised by two different platform regimes. They are the Platform Interior and the Platform Exterior each of which have unique sedimentary lithofacies zones that produce different types of lithofacies. Each lithofacies is characterised by special depositional conditions and palaeobathymetry that interact with sea level changes and the accommodation space. The important palaeoenvironments are intertidal, restricted lagoon (subtidal), open marine, deeper open marine, inner shoal, shoal and platform margin. Generating, and testing, a depositional model as a part of formulating a sequence stratigraphical interpretation of a region is a key to understanding its geological development and – ultimately – reservoir potential. The micropalaeontology and sedimentology of the Sulaiy Formation in the subsurface have indicated a succession of clearly defined shallowing−upwards depositional cycles. These typically commence with a deep marine biofacies with wackestones and packstones, capped with a mudstone-wackestone maximum flooding zone and an upper unit of packstone to grainstones containing shallow marine biofacies. The upper part of the Sulaiy Formation is highstand-dominated with common grainstones that host the Lower Ratawi reservoir which is capped by karst that defines the sequence boundary. This karst is identified by its abundant moldic porosity that enhanced the the reservoir quality by increasing its porosities into greater values. Integration of the sedimentology and micropalaeontology has yielded a succession of shoaling−upwards depositional cycles, considered to be 4th order sequences, that are superimposed on a large scale 3rd order system tract shallowing−upwards, highstand-associated sequence of the Sulaiy Formation. The Lower Ratawi Reservoir is located within the latest high-stand portion of a third-order Sulaiy Formation sequence. The reservoir consists of a succession of several sequences, each of which is sub-divided into a lower transgressive systems tract separated from the upper highstand systems tract by a maximum flooding surface (MFS/Z). The last of these depositional cycles terminates in beds of porous and permeable ooid, or ooidal-peloidal, grainstone. The reservoir is sealed by the finer-grained sediments of the Yamama Formation.