Thesis (M. Sc. (Microbiology)) -- University of limpopo, 2016 / Human activities are known to be the major contributors to contamination of natural water sources. This becomes a serious health risk when the communities rely on the same water sources for their household water needs. The current study investigated the bio-physicochemical quality of surface and ground waters found in a mining area in the Tubatse locality, for their suitability for drinking and other household use. The bacteriological analyses of surface waters showed dominance by genera of the phylum Proteobacteria, followed by Actinobacteria and Firmicutes. The isolates included the genera Aeromonas, Pseudomonas, Cronobacter, Acinetobacter, Enterobacter, Pantoea, Serratia, Bordetella, Kocuria and Streptococcus. This dominance pattern is proportionately similar to the pattern reported on human skin and of gut biota. Enterobacter spp. were the predominant species in the surface waters, followed by Bordetella spp. With regard to ground water, one sample was laden with coliforms whereas the other sample was free of coliforms. Physical quality parameters such as turbidity, colour and (total suspended solids) TSS of the surface and ground water samples were compliant with the set standards for drinking water according to South African water quality guidelines (2005). The concentrations of Zn, [SCN-], Cr, Co, Fe, Ni, Cu, H2O2, Cl2 were determined in the surface and ground waters in dry and wet seasons as well. A non-significant decrease in the levels of Cl2 and [SCN-] and an increase in TDS and Cr were observed in the wet season. All metal and chemical levels in surface and ground water, except Cr, were lower than permitted concentration for drinking water. However the concentrations of Zn, Fe and Co exceeded the normal expected concentrations of < 3.5 μg/L, 0.5 mg/L, < 0.01 mg/L and < 5 μg/L respectively. The sediments and soil samples were digested with aqua regia for Cu, Cr, Fe, Co and Zn analyses. An increase in the levels of Zn, Cr and Co in the wet season was observed. The concentrations of the heavy metals such as Co, Cu and Cr were higher in sediments at the sampling points closer to the mining sites. Cytotoxicity assay was performed with different concentrations, as detected in the water and sediment samples, of Zn, [SCN-], Cr, Co, and Fe on C2C12 (mammalian) cells. An increase in viable cells was observed after treatment with Cr (0.2, 0.45 and 0.9 mg/L), Zn and Fe (1.0 and 2 mg/L), Co (2, 5 and 10 mg/L). Only cells treated with SCN- (3.3, 5.4 and 7.2 mg/L) exhibited a significant decrease in viability. These results demonstrate that the water in the Tubatse municipality is not suitable for drinking and other household purposes without prior treatment which will remove contaminating microorganisms and chemicals and heavy metals.