Kiel, Univ., Diss., 2002. / Computerdatei im Fernzugriff.

Bochum, Universiẗat, Diss., 2001.

Thesis (Ph. D.)--Ohio State University, 1947. / Includes vita. Includes bibliographical references (leaves 97-99). Available online via OhioLINK's ETD Center.

Diss. (sammanfattning) Uppsala : Uppsala universitet, 2005. / Härtill 4 uppsatser.

Thesis (M.S.)--Ohio State University, 1931. / Includes bibliographical references (leaves 44-46). Available online via OhioLINK's ETD Center.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1930. / Typescript. Includes abstract. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references ([3] leaves at end).

Heidelberg, University, Diss., 2003. / Dateien im PDF-Format.

Normal and acid aluminum sulphate solutions containing about 6.0 gr/1 of aluminum and up to 50 gr/1 SO₄⁼ were hydrolyzed until equilibrium was reached in the temperature region 125-250°C. Under the equilibrium conditions the only stable solid phase observed in equilibrium with a liquid phase of various compositions was basic aluminum sulphate with nominal formula 3A1₂0₃..4S0₃.9H₂O. A small portion of the ternary diagrams for the system A1₂O₃-SO₃-H₂O at 225°C and 250°C was constructed. A mixture of aluminum sulphate and other metal sulphates, K₂S0₄, Na₂S0₄, Li₂SO₄, FeSO₄ and CuSO₄ i.e. was hydrolyzed at 225°C in order to find the effect of these salts on hydrolysis. The overall hydrolysis reaction was found to occur according to the chemical equation: 6A1⁺⁺⁺+ 4HS0₄⁻ + 14H₂O ⃗ 3Al₂0₃.4S0₃.9H₂0 + 14H⁺ The equilibrium constants at 125, 150, 175, 200, 225 and 250°C were determined. Finally a mechanism for the hydrolysis of aluminum sulphate was proposed. / Applied Science, Faculty of / Materials Engineering, Department of / Graduate

Syndecan-1 is a cell surface proteoglycan which participates in cell adhesion, differentiation, motility, morphogenesis and intracellular signaling. The two glycosaminoglycans heparan sulfate and chondroitin sulfate are covalently attached to the ectodomain of syndecan-1 via a tetra saccharide linkage sequence. However, the ectodomain can be modified having only one or neither of the glycosaminglycans attached. The glycosaminoglycans are capable of binding ligands such as fibroblast growth factors (FGFs) and support activation of receptors. The ectodomain is proteolytically cleaved from the cell surface by metalloproteinases in a process known as shedding. Shedding turns the ectodomain into a soluble effector which can stimulate other cells in the surroundings by delivering growth factors and also translocate into cells through endocytosis. In this study the aim was to find out if a modified ectodomain, which only contains chondroitin sulfate, could support intracellular signaling in the absence of heparan sulfate. The aim was also to find out whether a modified ectodomain could translocate into the cell. The methods used were cell culturing, isolation and purification of syndecan-1 ectodomain, cell signaling and immunohistochemistry. It was found that modified shed syndecan-1 ectodomain was able to support intracellular signaling almost to the same degree as wild type syndecan-1 ectodomain. This may suggest that heparan sulfate does not have to be present on the ectodomain to support intracellular signaling, although the signal is slightly higher when present. When trying to detect translocation of the ectodomain the results were too uncertain and further research is required.

Regeneration is, in simple terms, ‘to re-grow’ damaged or lost parts of the body (e.g. cells, tissues and organs) and is a natural phenomenon occurring throughout the life of an organism. The regenerative capacity varies in the animal kingdom. Invertebrates have high regenerative capacity in contrast to higher vertebrates. This raises several fundamental questions related to the regeneration potential, evolutionary selection and its cellular and molecular mechanisms. An in-depth knowledge in regeneration is warranted to answer the fundamental questions that are still a challenge in regenerative medicine.  Glycosaminoglycans (GAGs) are known to be involved in various physiological processes. Of several GAG types galactosaminoglycans are the focus of this thesis. Galactosaminoglycans such as chondroitin sulfate/dermatan sulfate (CS/DS) are anionic linear polysaccharides covalently linked to core proteins so called proteoglycans (PGs), and form an integral part of both cell surface and extracellular matrix components. Although CS/DS have been associated with different cellular processes from development to homeostasis, not many studies have been carried out to understand their role in regeneration. In this thesis, we aim to study galactosaminoglycans, their structure, and interaction with growth factors of biological importance in the process of regeneration using simple invertebrate model organisms - brittlestars. We have identified CS/DS as the major GAG present in brittlestars. Molecular characterization of these chains indicated a much higher level of sulfation in Amphiura filiformis than so far found in GAGs from invertebrates or vertebrates. This brittlestar CS/DS promotes FGF2 mediated cell signaling similar to heparin. Further, we studied the functional role of these CS/DS chains and their biosynthetic machinery during arm regeneration in A. filiformis. Regeneration is followed by an increase in GAG sulfation from blastema stage to the fully functional arm. Suppressing sulfation on the other hand by sodium chlorate treatment drastically affected the proliferation process and thereby regeneration. Thus our findings suggest a potential biological role of CS/DS in brittlestar limb regeneration that may have relevance to regenerative medicine in future.