Yu Chi Hung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 124-139). / Abstracts in English and Chinese. / Acknowledgement --- p.i / Table of Contents --- p.ii / Abbreviations --- p.iv / Abstract --- p.v / 論文摘要 --- p.vi / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- General introduction / Chapter 1.2 --- HBV and its potential oncogenic properties / Chapter 1.3 --- Aim of the present study / Chapter 1.4 --- Expressed sequence tag (EST) analysis: an approach to reveal gene expression pattern in a specific tissue / Chapter 1.5 --- cDNA subtraction / Chapter Chapter 2 --- Materials and Methods --- p.17 / Chapter 2.1 --- Plating out the adult human normal liver cDNA library / Chapter 2.2 --- PCR amplification of cloned human normal liver cDNA inserts / Chapter 2.3 --- Cycle sequencing of cloned human normal liver cDNA inserts / Chapter 2.4 --- mRNA preparation from the HCC tissue and its surrounding normal counterpart / Chapter 2.5 --- PCR-Select cDNA subtraction / Chapter 2.6 --- Construction of HCC subtracted cDNA library by T/A cloning method / Chapter 2.7 --- PCR amplification of cloned subtracted cDNA / Chapter 2.8 --- Cycle sequencing of cloned subtracted cDNA / Chapter 2.9 --- Sequence analysis / Chapter 2.10 --- Differential hybridization of HCC subtracted clones / Chapter Chapter 3 --- Results --- p.46 / Chapter 3.1 --- The sequencing results of adult human normal liver cDNA clones / Chapter 3.2 --- Categorization of ESTs sequenced from the adult normal liver / Chapter 3.3 --- Adaptor ligation efficiency analysis / Chapter 3.4 --- Primary and secondary PCR Amplification / Chapter 3.5 --- PCR analysis of subtraction efficiency / Chapter 3.6 --- The sequencing results of subtracted HCC cDNA clones / Chapter 3.7 --- Categorization of ESTs sequenced from the subtracted HCC cDNA library / Chapter 3.8 --- Differential hybridization of subtracted cDNA clones / Chapter Chapter 4 --- Discussions --- p.90 / Chapter 4.1 --- Characterization of the ESTs generated from human normal liver cDNA library / Chapter 4.2 --- EST analysis on subtracted HCC cDNA clones / Chapter 4.3 --- Candidate genes differentially expressed in HCC / Appendix A The coordinates of dot blots (in numerical order according to clone numbers) / Appendix B The coordinates of dot blots (in alphabetical order according to putative identity) / References --- p.124

Stephen Siu-chung Chim. / "June 2000." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (p. 152-166). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

The objectives of this research work are to investigate, design and implement active integrated antennas comprising active devices connected directly to the patch radiators, for various applications in high efficiency RF front-ends, integrated oscillator antennas, design and optimization of harmonic suppression antennas using a genetic algorithm (GA). A computer-aided design approach to obtain a class F operation to optimizing the optimal fundamental load impedance and designing the input matching circuits for an active integrated antenna of the transmitting type is proposed and a case study of a design for 1.6 GHz is used to confirm the design principle. A study of active integrated oscillator antennas with a series feed back using a pseudomorphic high electronmobility transistor (PHEMT) confirms the design procedure in simulation and measurement for the oscillator circuit connected directly to the active antenna. Subsequently, another design of active oscillator antenna using bipolar junction transistor (BJT) improves the phase noise of the oscillation and in addition to achieve amplitude shift keying (ASK) and amplitude modulation (AM) modulation using the proposed design circuit. Moreover, the possibility of using a sensor patch technique to find the power accepted by the antenna at harmonic frequencies is studied. A novel numerical solution, for designing and optimizing active patch antennas for harmonic suppression using GA in collaboration with numerical electromagnetic computation (NEC), is presented. A new FORTRAN program is developed and used for adaptively meshing any planar antenna structure in terms of wire grid surface structures. The program is subsequently implemented in harmonic suppression antenna design and optimization using GA. The simulation and measurement results for several surface structures show a good agreement.