Call number: LD2668 .T4 1948 K7 / Master of Science

Sweetpotato, Ipomoea batatas (L.) Lamarck was introduced into Lesotho in 1992 in the hope that it would help alleviate poverty levels. Efforts are being made to learn the potential constraints to optimal production of this crop. Insect pests especially the sweetpotato weevils, Cylas species are a major production constraint worldwide. Hence the main objective of the study was to predict if these pests have potential to establish themselves in Lesotho. Two Cylas species, C. formicarius and C. puncticollis are present in South Africa and the latter is found in the northern Free State and the Eastern Cape both of which border the central and southern lowlands of Lesotho respectively. Cylas puncticollis was chosen as the subject of this study because of its potential spread into Lesotho. Thermal requirements (lower development threshold and degree-days) of this pest were calculated in the laboratory by studying the effects of temperature on its development and survival at six constant temperatures (16°C, 19°C, 24°C, 26°C, 31°C and 36°C). The photoperiod was maintained at 12L:12D for all temperatures, but RH was not controlled. Thermal requirements (r and k) of this pest species were estimated for all the immature stages and for the total life-cycle using the linear regression method. The estimated lower temperature threshold (r) of the total development of the pest lies between 8°C and 12°C and the thermal constant (k) between 360°D and 380°D. The thermal needs of this pest obtained from the laboratory work were used to predict the potential for its establishment in Lesotho as well as determining the possible areas of distribution if it invades Lesotho. Actual soil temperatures to which the pest would be exposed to in Lesotho were recorded for a year. Both the calculated thermal needs of the pest and the field-recorded temperatures were used in the degree-day model to predict potential establishment of this pest. The second approach, climate matching in Geographical Information System (GIS) used the bio-climatic profile of C. puncticollis calculated from the known areas of its distribution in both South Africa and Swaziland. The bio-climatic profiles of the two countries were matched to the climatic conditions of Lesotho to predict the potential for its establishment. The two approaches, linear degree-day model and climate matching approach revealed that Cylas puncticollis is a potential pest in Lesotho. The former predicted the occurrence of this pest throughout the whole country with a maximum of eight generations per year being possible in the lowlands. Fewer generations (two to three) were predicted for the highlands and foothills agro-ecological zones, which are colder than the lowlands. The climate matching approach also confirmed the prediction although according to this method a patchy distribution of the pest was predicted. A survey was then carried out in Lesotho, first to determine if Cylas species were already present in Lesotho, secondly to identify any other pests of sweetpotato and lastly to determine other possible production constraints other than insect pests. The survey was conducted in the form of questionnaire and field sampling. Cylas species were neither documented by the farmers who were interviewed nor by the field sampling. Numerous common pests of sweetpotato were recorded during the sampling survey. These included the following leaf-feeding pests: Bedellia somnulentella Zeller, Acraea acerata Hewitson, Agrius convolvuli Linnaeus and locusts and grasshoppers. The root pests that were recorded were mole-rats, Blosyrus sp. and millipedes (Narceus sp.). According to the sampling carried out in Lesotho there were no insect pests that could be rated as major pests as yet. Sweetpotato farmers did not consider insect pests as an important production constraint for optimal yield of the crop. The major constraint was found to be lack of planting material, which contributed towards a slow adoption of the crop throughout the country. / Dissertation (MSc (Entomology))--University of Pretoria, 2007. / Zoology and Entomology / unrestricted

No description available.

Field experiments were conducted during the 1989 dry season (July to December) at the Manggoapi Farm of the Faculty of Agriculture, Cenderawasih University in Manokwari, Irian Jaya, Indonesia. The objectives of the experiments were to determine the effects of four sweet potato cropping systems on the population density of sweet potato weevils (SPW) and on the diversity of other insects within these agroecosystems. / Fewer SPW were found in intercropped sweet potato + corn (2 weevils per kg infected tubers), sweet potato + soybean (21 weevils), sweet potato + corn + soybean (8 weevils) than in monoculture sweet potato (37 weevils); percentage of damaged tubers followed the same trend, ranging from 2.6% to 14.0% in intercropped sweet potato, to 21.9% in the sweet potato monoculture. However, the higher number of SPW and damaged tubers in the monoculture did not reduce yield below that in the intercropped plots. / Insect and spider populations were more diverse in the intercropped sweet potato systems than in monoculture. Number of arthropods increased throughout the growing season. Intercropping may reduce the population density of other insect pests associated with sweet potato and may increase the population density of natural enemies.

Please read the abstract in the section 00front of this document / Dissertation (Magister Istitutiones Agrariae)--University of Pretoria, 2006. / Plant Production and Soil Science / unrestricted

No description available.

Thesis. (M. A. Agriculture (Agricultural Economics)) -- University of Limpopo, 2022 / Food security, poverty and hunger issues, as well as methods of addressing remain a concern for many South Africans. Smallholder farmers' agricultural production is seen as the key to simultaneously alleviating poverty and ensuring food security, especially in rural areas. The sweet potato crop is commonly produced by smallholder farmers in rural areas as a staple in many South African households with the potential to reduce hunger and poverty. Nevertheless, just like other crops, the sweet potato is impaired by external factors such as extreme weather conditions, insects, pests and diseases, thus threatening food security. The most destructive pest to sweet potatoes acknowledged in the literature is the sweet potato weevil (SPW), which can cause between 5-100% in areas where it is not controlled. While there are many SPW control measures Entomopathogenic Nematodes (EPNs) are emerging as one of the Integrated Pest Management (IPM) bio-control techniques that have shown promise in controlling SPW infestations in South Africa and globally. This study conducts a socio-economic analysis of smallholder sweet potato production and analyses the acceptability of EPNs as bio-control measures against the SPW in the Gauteng, Limpopo and North West Provinces of South Africa. This was done through an assessment of farmers‘ knowledge, attitudes, perception and practices (KAPP analysis), exploration of the acceptability of EPNs by farmers, determination of and factors influencing profitability and technical efficiency. Primary data was collected from 119 respondents who were selected through non-probability sampling techniques; purposive, census, and snowball. The analytical tools used to analyse the data were descriptive statistics, Gross Margin Analysis, Multiple linear regression model, Data Envelopment Analysis (DEA) and the Tobit regression model. From the results, an average knowledge score of 2.30 based on a 3–point Likert scale revealed that sweet potato farmers are knowledgeable of the SPW, the impacts and the control measures. Despite this level of knowledge, the farmers were impartial about the attitudes and perceptions regarding the SPW and the control measures. This was based on the findings of a 5-point Likert scale, which yielded average scores of 2.53 and 2.74, respectively. The study also revealed that the majority of the farmers prefer the use of indigenous and physical practices to control SPW. With regards to acceptance of the EPNs bio-control innovation towards control of the SPW, a mean Composite Index of Acceptancy (CIA) of 0.77 revealed the willingness of farmers to accept the EPNs as a bio-control measure. A Gross margin of R9 552.37 indicates that sweet potato farming is generally profitable, and this is influenced by socio-economic factors such as marital status, employment status, sweet potato output per cycle and access to machinery. On the other hand, while sweet potato farming was found to be profitable, the DEA score of 0.09 reveals that these farmers are technically inefficient. Their technical inefficiency is influenced by sweet potato output per cycle, gross margins, farm size, and access to credit, employment status, and chemical use. Based on these findings, the study recommends farmers‘ support through capacity development initiatives for the sweet potato farmers with regards to general economics of sweet potato production and marketing to maximise and sustain their revenue generation, as well as their general efficiency. In addition, increased training and awareness of the EPNs and their benefits as bio-control measures towards SPW infestation will work towards changing farmers‘ mindset with regard to SPW control measures. / Department of Social Innovation (DSI) and United States Agency for International Development (USAID)

Alternaria leaf petiole and stem blight is an important disease of sweetpotato (Ipomoea batatas (L.) Lam.) causing yield losses in both landraces and improved cultivars. The most important species causing economic yield loss in Uganda are Alternaria bataticola and A. alternate with A. bataticola the most aggressive and widely distributed. The study was conducted to: i) establish farmer-preferred sweet potato attributes, production constraints and Alternaria leaf petiole and stem blight awareness; ii) evaluate Ugandan sweet potato germplasm for Alternaria leaf petiole and stem blight resistance; iii) determine the mode of inheritance of resistance to Alternaria leaf petiole and stem blight and storage root yield components of sweet potato through estimation of the general combining ability (GCA) of the parents and the specific combining ability (SCA) of the parents for each cross; and iv) determine the adaptability and farmer acceptability of selected F1 genotypes across environments. The participatory rural appraisal was conducted to establish farmer preferences and production constraints revealed that farmer preferred sweet-potato traits were high yield, sweetness (taste), early maturity, high dry mass, resistance to pests and diseases, and in-field root storability after maturity. A majority of the farmers considered Alternaria leaf petiole and stem blight a serious production constraint causing yield loss of over 50%. The main control measures against the disease were roguing of infected plants, spraying with fungicides, use of healthy planting materials and planting resistant genotypes. Thirty sweet potato land races and improved cultivars were evaluated for Alternaria blight severity; yield, dry mass, harvest index, sweetpotato weevil (Cylas spp.) damage and sweetpotato virus disease at two sites (Namulonge and Kachwekano) over three seasons (2010B, 2011A, 2011B) under Alternaria inoculum and fungicide spray treatments. Landrace Shock was more resistant to Alternaria blight than Tanzania, the resistant check. Genotypes NASPOT 1, NASPOT 7, New Kawogo and Dimbuka were the most susceptible. Thirty two F1 families were generated from 16 parents in two sets in a North Carolina II mating scheme. The families were evaluated at two sites using a 5 x 7 row-column design with two replications. There were significant (P<0.05) differences among the families in Alternaria blight severity. Both GCA and SCA mean squares (MS) for Alternaria blight were highly significant (P<0.001) but the predominance of GCA sum of squares (SS) for Alternaria blight at 67.4% of the treatment SS versus 32.6% for SCA SS indicated that additive effects were more important than the non-additive effects in controlling this trait. For the yield components, the GCA MS were significant (P<0.05) and accounted for more than 60% of the treatment SS except for percentage dry mass composition where SCA SS accounted for 53.0% of the treatment SS implying that non-additive genetic effects were slightly more important than additive for this trait. Some parents that had desirable high, negative GCA effects for Alternaria blight produced families with undesirable positive SCA effects and the reverse was also true. This implied that the best parents should not be chosen based on GCA effects alone but also on SCA effects of their best crosses. The promising F1 genotypes selected from previously evaluated crosses together with one Alternaria blight resistant check (Tanzania) and one susceptible check (NASPOT 1) were evaluated at three sites (Namulonge, Kachwekano and Serere) using a randomised complete block design with three replications. Scientists and farmers evaluated the agronomic performance and also quality traits of the genotypes before and at harvest. Genotypes G14, G16, G24, G29, G49, G59 and G69 were the most stable across the sites for low Alternaria blight severity and can, therefore, be recommended for further evaluation under both low and high disease pressure areas. Genotypes G67, G13, G14, G24, G29 and G53 were the most high yielding and stable across the sites and were therefore the most widely adapted. In the participatory selection, before harvest and at harvest, Spearman’s rank correlation of the scientists and farmers’ mean ranking of the genotypes at each site was positive and significant. This indicated that the scientists in the study were capable of selecting for farmer preferred traits. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.

Thesis (M. Sc. (Plant Protection)) -- University of Limpopo, 2021 / Worldwide, root-knot (Meloidogyne species) nematodes are considered to be the most important and damaging genus in crop husbandry. The existence of a wide host range, over 2000 plants, and several biological races, makes the management of this nematode genus difficult with nematode-resistant crop Hybrid Sorghum Sudan grass (Sorghum bicolor × Sorghum Sundanese) has been classified as being resistant to certain Meloidogyne species and races, with a wide range of uses in crop rotation intended to manage nematode population densities. However, due to the ability of nematodes to enter chemiobiosis when gradually exposed to chemicals, this hybrid might not be effective in managing nematode population densities for the subsequent highly susceptible sweet potato (Ipomoea batatas L.) cultivars. The objective of the study was to determine whether hybrid Sorghum-Sudan grass would suppress M. javanica (Trial 1), M. incognita race 2 (Trial 2) and M. incognita race 4 (Trial 3) population densities, allowing a nematode susceptible sweet potato cv. ′Beauregard′ as successor crop to be cultivated without suffering nematode damage. The hybrid Sorghum-Sudan grass study was conducted under greenhouse conditions, with seven inoculation levels, namely, 0; 5; 25; 125; 625; 3 125 and 15 625 eggs and second-stage juveniles (J2) of each nematode species or race, arranged in randomised complete block design, with six replications and validated in time. Plant growth, foliar nutrient elements and nematodes were collected at 56 days after inoculation and prepared for analysis using standard methods. The reproductive factor (RF) at all levels was zero, whereas nematode inoculation at all levels did not have any effect on plant growth of the hybrid Sorghum-Sudan grass. However, the nematode levels affected the accumulation of nutrient elements and the quality of forage. After cultivating the susceptible sweet potato cultivar in pots xxx previously with hybrid Sorghum-Sudan grass at increasing levels of M. javanica alone, that is in Trial 1, similar results were observed with respect to RF and lack of nematode damage to plant growth. Consequently, the hybrid was suitable for use in crop rotation with sweet potato for the purpose of managing nematode population densities of thermophilic Meloidogyne species and/or races. / National Research Foundation of South Africa (NRF) and the Agricultural Research Council (ARC)

Sweet potato (Ipomoea batatas Lam.) is ranked as the seventh most important food crop in the world and its large biomass and nutrient production give it a unique role in famine relief. However, multiple virus infection is the main disease limiting factor in sweet potato production worldwide. The main objective of this research project was to develop a transgenic sweet potato cultivar with broad virus resistance in South Africa (SA). A review of current literature assembled background information pertaining to the origin, distribution and importance of the sweet potato crop; viruses and complexes infecting sweet potato; and the strategies used in sweet potato virus detection and control. A survey to determine the occurrence and distribution of viruses infecting sweet potato (Ipomoea batatas Lam.) was conducted in major sweet potato-growing areas in KwaZulu-Natal (KZN). A total of 84 symptomatic vine samples were collected and graft inoculated onto universal indicator plants, Ipomoea setosa Ker. and Ipomoea nil Lam. Six weeks post inoculation, typical sweet potato virus-like symptoms of chlorotic flecking, severe leaf deformation, stunting, chlorotic mosaic, and distinct interveinal chlorotic patterns were observed on indicator plants. Under the transmission electron microscope (TEM), negatively stained preparations of crude leaf sap and ultra-thin sections from symptomatic grafted I.setosa plants revealed the presence of elongated flexuous particles and pinwheel type inclusions bodies‟ that are characteristic to the cytopathology of Potyviruses. Symptomatic leaf samples from graft-inoculated I. setosa and I. nil were assayed for Sweet potato feathery mottle virus (SPFMV), Sweet potato mild mottle virus (SPMMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato chlorotic fleck virus (SPCFV), Sweet potato virus G (SPVG), Sweet potato mild speckling virus (SPMSV), Sweet potato caulimo-like virus (SPCaLV), Sweet potato latent virus (SPLV), Cucumber mosaic virus (CMV), and Sweet potato C-6 virus (C-6) using the nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). The majority of leaf samples (52%) tested positive for virus disease and showed the occurrence of SPFMV, SPMMV, SPCSV, SPCFV, SPVG, SPMSV, and SPCaLV. Of these 7 viruses, the most frequently detected were SPFMV (39%), SPVG (30%), followed by SPCSV (13%) and SPMMV (12%). SPCaLV and SPCFV at 10% and SPMSV at 7% were found exclusively in samples collected from one area. SPFMV, SPVG, SPCSV, and SPMMV were identified as the most prevalent viruses infecting sweet potato in KZN. The genetic variability of the three major viruses infecting sweet potato (Ipomoea batatas Lam.) in KZN was determined in this study. A total of 16 virus isolates originating from three different locations (Umbumbulu, Umfume and Umphambanyomi River) in KZN were analyzed. These comprised of 10 isolates of Sweet potato feathery mottle virus (SPFMV), five isolates of Sweet potato virus G (SPVG) and one isolate of Sweet potato chlorotic stunt virus (SPCSV). The phylogenetic relationships of the SPFMV, SPVG and SPCSV isolates from KZN relative to isolates occurring in SA and different parts of the world were assessed. The division of SPFMV into four genetic groups (strains) according to the phylogenetic analysis of coat protein encoding sequences revealed mixed infections of the O (ordinary) and C (common) strains in sweet potato crops from KZN. All SPFMV isolates showed close lineage with isolates from South America, East Asia and Africa. The SPVG isolates showed high relatedness to each other and close lineage with other isolates, especially those from China and Egypt. Analysis of the partial sequence of the Heat shock protein 70 homologue (Hsp70h) gene indicated that the SPCSV isolate from KZN belongs to the West African (WA) strain group of SPCSV and showed close relatedness to an isolate from Argentina. The knowledge of specific viral diversity is essential in developing effective control measures against sweet potato viruses in KZN. Multiple virus infections of Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Sweet potato virus G (SPVG) and Sweet potato mild mottle virus (SPMMV) cause a devastating synergistic disease complex of sweet potato (Ipomoea batatas Lam.) in KZN. In order to address the problem of the multiplicity and synergism of sweet potato viruses in KZN, this study aimed to develop transgenic sweet potato cv. Blesbok with broad virus resistance. An efficient and reproducible plant regeneration protocol for sweet potato (Ipomoea batatas Lam.) cultivar Blesbok was also developed in this study. The effect of different hormone combinations and type of explants on shoot regeneration was evaluated in order to optimize the regeneration protocol. Coat protein (CP) gene segments of SPFMV, SPCSV, SPVG and SPMMV were fused to a silencer DNA, the middle half of the nucleocapsid (N) gene of Tomato spotted wilt virus (TSWV) and used as a chimeric transgene in a sense orientation to induce gene silencing in the transgenic sweet potato. Transformation of apical tips of sweet potato cv. Blesbok was achieved by using Agrobacterium tumefaciens strain LBA4404 harboring a modified binary vector pGA482G carrying the plant expressible neomycin phosphotransferase ll gene (nptll), the bacterial gentamycin-(3)-N-acetyl-transferase gene and the expression cassette. A total of 24 putative transgenic plants were produced from the transformed apical tips via de novo organogenesis and regeneration into plants under 50mg/L kanamycin and 200 mg/L carbenicillin selection. Polymerase chain reaction (PCR) and Southern blot analyses showed that six of the 24 putative transgenic plants were transgenic with two insertion loci and that all plants were derived from the same transgenic event. The six transgenic sweet potato plants were challenged by graft inoculation with SPFMV, SPCSV, SPVG and SPMMV- infected Ipomoea setosa Ker. Although virus presence was detected using NCM-ELISA, all transgenic plants displayed delayed and milder symptoms, of chlorosis and mottle of lower leaves when compared to the untransformed control plants. These results warrant further investigation under field conditions. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2013.