This thesis presents an evaluation of the effectiveness of an agroforestry extension program to smallholder farmers on Leyte Island, the Philippines. The imperative for reforestation is well recognised in the Philippines and was the impetus for this program which provided farmers with assistance to establish and silviculturally manage timber trees on their land. Because the cost-effectiveness of agroforestry extension is increased if farmers develop self-efficacy without extensive training, the extension program was offered in two regimes to test the necessity for extended assistance. In the extended assistance regime, farmers were offered on-site assistance to collect seed, grow seedlings, prepare sites and establish trees, whereas in the limited assistance regime, farmers were only offered assistance to collect seed and grow seedlings. Descriptive statistics were collected of farmers’ acceptance of technology and the manner in which technology was adapted to suit their personal circumstances. Translated conversations between farmers and extension staff also provided a rich source of data which provided insights into farmers’ motivation. Extension activities were reviewed at a mid-program workshop, a final on-site inspection and an end-of-program workshop. Farmers responded positively to the extended assistance program which helped them to grow and out-plant seedlings. The limited assistance program was relatively unsuccessful. Overall, the extension program was successful in shifting the initiative for further planting from extension staff to participating farmers. However, farmers showed little interest in applying silvicultural thinning or pruning to existing plantations of trees because extension advice was not congruent with their existing mental models of these procedures. Systems modelling of socio-economic variables which had been found to affect program outcomes was used to predict critical success factors. A key constraint to program recruitment was found to be farmers’ perception of harvest security, even when their needs for technology and planting materials are met. Modelling also cast doubt on the usefulness of written extension materials and emphasised the necessity for extended face-to-face technical assistance. Although conducted in Leyte, the findings of this research provide guidance for issues which affect the adoption of agroforestry both in the Philippines and in other countries. The research found that it was possible to recruit and motivate farmers without providing material incentives. If farmers experienced unexpected problems, providing extended face-to-face contact and assistance was critical if catastrophic losses of participating farmers were to be avoided. The failure of attempts to introduce advanced-age silviculture also indicated a need to elicit farmers’ mental models as a precursor or parallel enquiry to extension activities. In a situation where little was initially known about farmers’ understanding of agroforestry technology or the variables which affect their acceptance or rejection of extension assistance, the results of this research have shown that it is possible to build the capacity of farmers to establish timber trees. This result is in contrast to the acknowledged failure of the logging concession system in the Philippines and the difficulties faced by some industrial plantations and community-based programs. This investigation has shown that an opportunity exists to lift the level of tree planting in Leyte, provided that system variables which are either critical success factors or impediments are addressed.

A família Meliaceae compreende cerca de 51 gêneros e 550 espécies distribuídas principalmente na região Neotropical. Incluídas nesta família, estão espécies de elevado interesse comercial para a produção de madeiras nobres. Há carência de informações quanto à biologia floral dessas espécies, que aliada a problemas silviculturas dificulta a elaboração de programas efetivos de melhoramento genético das espécies de interesse econômico da família. O presente trabalho visa contribuir para o entendimento do desenvolvimento floral na família Meliaceae, com destaque para sete espécies de interesse econômico e/ou ecológico: Cedrela fissilis L., Cedrela odorata L., Swietenia macrophylla R. A. King, Trichilia claussenii C. DC., Guarea guidonea (L.) Sleumer, Toona ciliata M. J. Roem e Melia azedarach L. Para os estudos morfo-anatômicos utilizaram-se técnicas de microscopia óptica e microscopia eletrônica de varredura. Analisou-se igualmente o padrão de expressão de genes do modelo ABC, relacionados ao desenvolvimento floral, via hibridização in situ. Os estudos morfo-anatômicos permitiram caracterizar o desenvolvimento dos primórdios dos órgãos florais e o estabelecimento de estágios arbitrários de desenvolvimento, auxiliando na caracterização da expressão gênica. Estes resultados permitiram ainda a identificação de flores funcionalmente femininas ou masculinas em S. macrophylla C. fissilis, C. odorata e T. ciliata, as quais apresentam dimorfismo sexual. O padrão de expressão dos homólogos dos genes: APETALA1 (AP1), APETALA3 (AP3) e AGAMOUS (AG) foram diferentes daqueles previstos pelo modelo ABC. A expressão dos homólogos de AP1 foi verificada em todo o meristema floral nos estágios iniciais de desenvolvimento, em todas as espécies estudadas. O sinal de expressão concentrou-se nos primórdios das sépalas e pétalas em estágios mais avançados do desenvolvimento, porém um fraco sinal de hibridização de AP1 foi verificado em todos os verticilos. A expressão dos homólogos de AP3 foi verificada nas regiões previstas pelo modelo ABC, correspondentes ao segundo e terceiro verticilos. Porém um fraco sinal de AP3 foi também observado no quarto verticilo, o que não é previsto pelo modelo teórico. A expressão dos homólogos de AG foi restrita à região central do meristema floral, correspondente ao terceiro e quarto verticilos, em todos os estágios do desenvolvimento floral de todas as espécies estudadas. Entretanto, sinais adicionais de hibridização dos homólogos de AG foram visualizados na região abaxial dos primórdios de sépalas e pétalas em C. fissilis, C. odorata e T. ciliata. Os estudos da expressão dos genes do modelo ABC em Meliaceae revelaram padrões de expressão que não concordam com o modelo teórico vigente do controle molecular da determinação da identidade dos órgãos florais._________________________________________________________________________________________ ABSTRACT: The Meliaceae family comprises approximately 51 genus and 550 species mainly distributed in the Neotropical region. In this family there are many species of commercial interest for the production of noble wood. The lack of information on the floral biology associated with silvicultural problems limits the elaboration of effective breeding programs with species of economic interest. The present work aims to contribute with the understanding of floral development in Meliaceae, focusing in seven species of economical or ecological interest: Cedrela fissilis L., Cedrela odorata L., Swietenia macrophylla R. A. King, Trichilia claussenii C. DC., Guarea guidonea (L.) Sleumer, Toona ciliata M. J. Roem e Melia azedarach L. Morpho-anatomical analyses were done by light and scannning electron microscopy. The expression pattern of the ABC model genes, which are related to floral development, was analyzed by in situ hybridization. The characterization of the development of floral organ primordia and the establishment of arbitrary stages of floral development was done and was important for the characterization of gene expression. These results allowed for the identification of functionally female and male flowers in Swietenia macrophylla, Cedrela fissilis, Cedrela odorata and Toona ciliata, characterizing sexual dimorphism. The patterns of gene expression of the homologous of APETALA 1, APETALA 3 and AGAMOUS were different from those predicted by the ABC model. AP1 expression was observed in the entire floral meristem in the initial stages of development, in all seven species. The expression signal was more concentrated in sepal and petal primordia in further stages of development, although a weak hybridization signal of AP1 was verified in all four whorls. The expression of AP3 homologs was observed in the second and third whorls, as predicted by the ABC model. However, a weak signal of AP3 was also observed in the forth whorl, which was not predicted by the ABC model. AG homolog expression was restricted to the central region of the floral meristem, corresponding to the third and forth whorls, in all stages of development of all seven species. However, additional hybridization signal of AG homologs were seen in the abaxial region of sepal and petal primordia in Cedrela fissilis, Cedrela odorata and Toona ciliata. The analyses of the ABC model genes in Meliaceae revealed that the expression patterns do not agree with the theoretical currently accepted model that determines the molecular control of the identity of the floral organs.