This thesis is focused on the evaluation of traditional chinese medicine (TCM) assortment of plants in the experimental garden ZF MENDELU. 51 species were evaluated by their aesthetic qualities. 43 species were used for harvesting and processing of drugs. These were described botanically and new information of the content of substances, the use and dosage of drugs were collected. The practical part of theses was scoped to the measurement of chemical substances content in plants. Total amount of flavonoids (TFC), phenols (TPC) and total antioxidant capacity (TAC) by FRAP and DPPH method has been investigated. For 13 plants was determined yield of essential oil. For species used in fresh state, concentration of vitamin C has also been determined. Some annual and perrenial species has been added to the assortment of cultivated plants. The list of plant species TCM, which are perspective for cultivation in the Czech Republic, has been also created.