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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

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A Rapid Method for the Purification of RNA Polymerase Holoenzyme From Escherichia Coli

Mehrpouyan, Majid, Champney, W. Scott 01 January 1990 (has links)
A method is described for the rapid purification of RNA polymerase holoenzyme from small amounts of Escherichia coli cells. Chromatography of a crude extract on a single-stranded DNA agarose column followed by gell filtration chromatography gave 95% pure holoenzyme. The enzyme kinetic characteristics on T7 DNA identical to those of RNA polymerase purified by other more laborious procedures.
affinity chromatography
RNA polymerase holoenzyme
RNA polymerase purification
T7 transcription assay
Biomedical Sciences

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