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An evaluation of the use of tritiated thymidine in chromosome studiesKrause, Margarida Oliveira. January 1960 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1960. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Thymidine nucleotide metabolism in Neurospora crassa and other organisms : problems of specific isotype labelling of DNA in fungiGrivell, Anthony Robert January 1970 (has links)
viii, 181 leaves : ill., appendix / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.1972) from the Dept. of Agricultural Biochemistry, University of Adelaide
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Thymidine nucleotide metabolism in Neurospora crassa and other organisms : problems of specific isotype labelling of DNA in fungi.Grivell, Anthony Robert. January 1970 (has links) (PDF)
Thesis (Ph.D. 1972) from the Dept. of Agricultural Biochemistry, University of Adelaide.
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Radiosynthesis of various pyrimidine derivatives and determining their uptake into cellsTaleli, Lebusetsa January 2009 (has links)
Thesis (MTech (Chemistry))--Cape Peninsula University of Technology, 2009 / N3-substituted pyrimidine nucleoside derivatives containing either an iodovinyl moiety or an
allyl group, i.e. [121]-N3-(3-iodoprop-2-en- l -yl)thymidine, (1~-711 and e21]-N3-(prop-2-enl-
yl)-5-iodo-Z'-deoxyuridine, (1~_10, were synthesised and preliminarily evaluated by
determining their uptake into CHO-Kl cells. Compound e~-711 was designed to be a
delivery vector of 121: into the DNA of the cells, while (1~_10 was designed to serve as a
control. Compound (1231]-711 was also intended to have a higher metabolic radiochemical
stability than 5-iodo-Z'-deoxyuridine ([123I]_IUdR) for the therapeutic use in cancer. The
synthesis of the N3-substituted intermediate precursors 411, 5 and 9 was achieved by Nalkylation
of suitably' protected thymidine and 5-iodo-Z'-deoxywidine analogues,
respectively. The intmediate precursors for radiolabelling, 411 and 11, were obtained by
incorporating a trialkylstannyl group at the respective labelling positions prior to
radioidination. (1~1-711 was recovered from (1~_6G after acid-hydrolysis of the protecting
groups and 10 was obtained from direct oxidative iodination of11. The radiochemical yields
ranged from 73% to 91% at the end ofthe synthesis and radiochemical purities were in excess
of99"10 after HPLC purification.
The cell-uptakes ofthe radiotracers were carried out and assessed by a direct comparison with
the gold standard e23I]-IUdR, which is known to bephosphorylated and incorporated into the
DNA of cells during the cells S-phase. The cell-uptake results of (1~_711 and (1~-10 were
roughly 4% and 3% relative to [123I]-IUdR, respectively. The poor cell-uptake of (1~_10
suggested thatthe uptake into the cells is not influenced by the position of the iodine atom in
the molecule, but most probably by the availability of the N3-position in its non-substituted
form. As a result of its poor incorporation into cells, it was concluded that the synthesised
radiotracer (1231]_711 would be a poor candidate for use in the eradication ofmalignant cells.
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Structural and functional dissection of the vaccinia virus thymidine kinase enzymeBlack, Margaret E. 30 April 1991 (has links)
Thymidine kinase is a key enzyme in the nucleotide salvage pathway,
catalyzing the production of dTMP from thymidine and ATP. In order to
identify the structural features of the TK protein and/or primary amino
acid sequences which contribute to the catalytic and regulatory activities of
this enzyme, an in vitro transcription and translation system has been used
in concert with protein engineering techniques for the production and
phenotypic characterization of mutant and wild-type TK enzymes. Because
of discrepancies in the literature regarding the quaternary structure of the
VVTK, the native molecular weight and quaternary structure was
determined to be an 80kDa homotetrameric enzyme by glycerol gradient
fractionation, gel filtration and glutaraldehyde cross-linking analyses.
Computer-assisted alignment of the predicted amino acid sequences
derived from cellular and poxvirus TK genes identified seven highly-conserved
domains distributed throughout the VVTK polypeptide
(domains I-VII). Domain I (amino acid residues 11-18 ) exhibits a high
degree of similarity to both ATP and GTP binding site consensus sequences,
although the VVTK utilizes only ATP as a phosphate donor. Site directed
mutagenesis and ATP-agarose affinity chromatography techniques were
employed to confirm that this region was responsible for ATP binding and
to determine which amino acids were essential for efficient binding.
The TK gene (tdk) from E. coli was isolated and sequenced to serve as
a prokaryotic enzyme with which to compare VVTK. The alignment
revealed only 23% shared identity with VVTK and, interestingly, the
identical and similar residues were clustered into three of the seven
domains identified previously (domains I, III and VII).
Preliminary evidence supports domain III (residues 78-90) as a
putative magnesium binding site and that a highly conserved cysteine
residue (cysteine 170) within domain VII (residues 168-171) may be a
component of the catalytic site. Secondary structure alignment between
Herpes Simplex Virus (HSV) TK and monkeypox TK (a close relative of
VVTK) revealed that the putative nucleoside binding site of HSVTK aligns
with residues within domain IV. Replacement of a VVTK residue (Q114)
with the corresponding residue of HSVTK (an aspartic acid) greatly alters
the substrate specificity and dTTP sensitivity of VVTK. / Graduation date: 1991
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Synthesis and gene silencing activity of RNA duplexes containing 3'-deoxy-3'-thiothymidineIsaac, Siara Ruth, January 1900 (has links)
Written for the Dept. of Chemistry. Title from title page of PDF (viewed 2009/06/25). Includes bibliographical references.
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Structural analysis of thymidylate synthase in nematodes: ascaris suum & caenorhabditis elegansTian, Li, 田莉 January 1998 (has links)
published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
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Structural analysis of thymidylate synthase in nematodes : ascaris suum & caenorhabditis elegans /Tian, Li, January 1998 (has links)
Thesis (Ph. D.)--University of Hong Kong, 1998. / Includes bibliographical references (leaves 215-242).
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Analyses of the thymidylate synthase promoter and an RNA helicase required for mRNA exportKapadia, Fehmida, January 2005 (has links)
Thesis (Ph. D.)--Ohio State University, 2005. / Title from first page of PDF file. Document formatted into pages; contains xvii, 179 p.; also includes graphics. Includes bibliographical references (p. 155-179). Available online via OhioLINK's ETD Center
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A mechanistic study of the preferential photo-oxidation of the 5-CH3 substituent of thymine and thymidine mediated by NH4(VO(O2)2(5-NO2phen))Wang, Jin 01 January 2009 (has links)
No description available.
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