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The analysis of signalling pathways in sporadic colorectal carcinoma using tissue microarraysMckenzie, Gavin, Medical Sciences, Faculty of Medicine, UNSW January 2008 (has links)
Colorectal carcinoma arises through sequential genetic changes whereby an adenoma develops from normal colonic epithelium and then becomes a carcinoma. Critical to this process is two forms of mutually exclusive genomic instability ?? chromosomal instability (CIN) and microsatellite instability (MSI). The colorectal tumours that develop from each of these pathways have distinct pathological and molecular differences. Most MSI+ colorectal carcinomas are associated with the CpG island methylator phenotype (CIMP) - an epigenetic phenomenon where a specific and consistent group of genes are silenced through promoter methylation. However, over half of fall CIMP+ colorectal tumours are microsatellite stable (MSS). It is well known that the WNT/β-catenin signalling pathway is instrumental in the initiation and development of CIN type tumours but it is less clear whether this pathway has any significant involvement in MSI+ or methylated tumours. The role of the PI3K1AKT signalling pathway in the development of solid human tumours has only recently been established and the affects of abnormal PI3K/AKT signalling in sporadic colorectal carcinomas is yet to be fully elucidated. The objective of this thesis was to investigate the involvement of the WNT/β-catenin and PI3K/AKT signalling pathways in the CIN, MSI+ and methylated subgroups of sporadic colorectal carcinoma. To achieve this, the expression patterns of β-catenin, p-AKT and PTEN were identified by immunohistochemistry on sections from tissue microarrays consisting of cores from a large group of sporadic colorectal carcinomas. Each of these proteins is an integral part of the constitutive activation of WNT/β-catenin or PI3K/AKT signalling and their expression patterns were correlated with the clinical, pathological and molecular characteristics of the different subgroups of colorectal carcinoma. Increased nuclear β-catenin expression, an indicator of activated WNT signalling, is associated with MSS and the pathological features of CIN type tumours and inversely associated with the pathological and molecular features of MSI+ and CIMP+ tumours. In all forms of sporadic colorectal carcinoma, nuclear β-catenin expression was not an indicator of overall survival. PTEN was not associated with any particular subgroup of sporadic colorectal carcinoma, but decreased cytoplasmic expression was indicative of overall worse outcome, especially in MSS or CIN type tumours. While the identification of nuclear β-catenin in sporadic colorectal carcinomas is not a satisfactory prognostic marker, the immunohistochemical detection of absent PTEN expression may prove useful in identifying poor outcome in individuals with sporadic MSS colorectal carcinoma.
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De novo morphogenesis on tomato thin cell layers and variation for genetic recombination among plantlets regenerated from tissue culture /Compton, Michael E., January 1990 (has links)
Thesis (Ph. D.)--Virginia Polytechnic Institute and State University, 1990. / Vita. Abstract. Includes bibliographical references. Also available via the Internet.
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Stimulation of bone marrow stromal cells in the development of tissue engineered ligaments /Moreau, Jodie E. January 2005 (has links)
Thesis (Ph.D.)--Tufts University, 2005. / Adviser: Gregory H. Altman. Submitted to the Dept. of Biology--Biotechnology. Includes bibliographical references (leaves 183-192). Access restricted to members of the Tufts University community. Also available via the World Wide Web;
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Silk polymer templates in biomineralization /Li, Chunmei. January 2005 (has links)
Thesis (Ph.D.)--Tufts University, 2005. / Advisers: David L. Kaplan; Gregory D. Botsaris. Submitted to the Dept. of Chemical and Biological Engineering. Includes bibliographical references. Access restricted to members of the Tufts University community. Also available via the World Wide Web;
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Neuartige Vliesstrukturen für den Einsatz in der Zell- und Gewebezüchtung /Wiesemann, Ute. January 2006 (has links)
Zugl.: Aachen, Techn. Hochsch., Diss., 2006.
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Applications of microdialysis in studies of the adrenergic regulation of lipolysis in human skeletal muscle and adipose tissue /Enoksson, Staffan, January 1900 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 5 uppsatser.
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Microdialysis sampling from skeletal muscle and adipose tissue with special reference to the effects of insulin on tissue blood flow and glucose metabolism /Rosdahl, Hans, January 1900 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 9 uppsatser.
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Intracranial and intraocular grafting of fetal ventral mesencephalic and striatal tissues : neuronal survival and nerve growth characteristics /Björklund, Lars, January 1900 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 7 uppsatser.
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Human spinal cord transplantation : experimental and clinical application /Åkesson, Elisabet, January 1900 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 6 uppsatser.
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An evaluation of elastic and oxytalan staining fibres in the temporomandibular joint and knee joint of the rabbit.Duthy, Lester Milton. January 1972 (has links) (PDF)
Thesis (M.D.S. 1973) from the Department of Dental Health, University of Adelaide.
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