The following were examined by taking advantage of TRDP using two-phase separation mixed solution: protein separation by the TRDC with the ternary mixed solution of water–acetonitrile–ethyl acetate, optical isomers separation by the TRDC with the ternary solution containing cyclodextrin as a chiral recognition molecule, development of the TRDC with a water–acetonitrile mixed solution containing sodium chloride as a two-phase separation mixed solution instead of the ternary solution, and introduction of the principle of the TRDC to a commercial HPLC system. Furthermore, the peroxioxalate CL detection was for the first time examined by using the ternary mixed solution. The investigation of the TRDP and TRDC, as well as the ternary mixed solution of water–acetonitrile–ethyl acetate, are interesting in view of not only analytical chemistry but also chemical engineering, and physical chemistry. / 博士(工学) / Doctor of Philosophy in Engineering / 同志社大学 / Doshisha University