The aim of the study was to assess the effects of orally applied praziquantel on the treatment efficacy against most abundant tapeworms (Atractolytocestus huronensis Anthony, 1958 and Khawia sinensis Hsü, 1935) of common carp (Cyprinus carpio Linnaeus). Naturally infected fish (one-year-old; K1+) were divided into 3 trial groups containing 17 specimens and administered with praziquantel mixed in heat-treated amyloid vehicle (in doses of 50 mg.kg-1 body weight). In 3 groups of control, the fish were administered amyloid vehicle only. Fish were examined by using of gut dissection 2, 4 and 6 days after administration. At 48 hours post-treatment, significantly lower prevalence of both parasites was found. The invasion of A.huronensis was fully eliminated in all fish after 96 hours whereas some specimens of K.sinensis still persisted in the gut of few fish. The full elimination of both parasites was observed 6 days after administration. Our results showed that elimination of K. sinensis takes longer than elimination of A.huronensis. The dose of 50 mg kg-1 of orally applied praziquantel is effectual to control A.huronensis and K sinensis infections in common carp successfully. The effect of oral applied praziquantel on the hematology and biochemical parameters blood of common carp (Cyprinus carpio L.), infected by the tapeworms Atractolytocestus huronensis and Khawia sinensis was the object of analyse. It was the partial goal of the work. The infected fishes were divided to the six groups. The praziquantel was applied stirred in amyloid vehicle in dose 30 mg/kg of quick materiality for two groups and for two to other groups in dose 50mg/kg of quick materiality. Only clean amyloid vehiculum was applied to two control groups. Blood taking and resulting patoanatomic investigation was effected 24 and 96 hours after application of praziquantel. Results of hematology investigation of blood obtained 24 hours after the application point on significant fall (p< 0,01) of the number of erythrocytes, hemoglobin and hematocrit in both groups E1 and E3, which achieved the original surface after 96 hours. The significant upsurge (p<0,05) of the surface serum hepatic enzyme ALT was recorded after 96 hours.

The use of intestinal parasites as indicators of pollution has increased in Europe due to the fact that parasites accumulate more of the available metals than their hosts. Eighty Largemouth yellowfish, Labeobarbus kimberleyensis (Gilchrist and Thompson, 1913) were collected between April 2005 and February 2006 with gill nets at RAU Island (26° 52, 249’ S, 28 10, 249’ E) in the Vaal Dam. The fish were killed, weighed and their lengths determined. Muscle, liver and spinal cord tissues were collected from each fish and the intestines removed and opened to expose Bothriocephalus acheilognathi. The position of attachment of B. acheilognathi in the host’s intestine was recorded. Thereafter tapeworms were collected in glass bottles and frozen. Water and sediment samples were collected. Clossed vessel microwave digestion was used to digest liver, muscle and tapeworms with Suprapur Nitric acid, and spinal cord tissue with Suprapur Nitric acid and Suprapur Hydrochloric acid. Thereafter metal concentrations of twenty three elements (Lithium, Beryllium, Titanium, Vanadium, Chromium, Manganese, Iron, Cobalt, Nickel, Copper, Zinc, Arsenic, Selenium, Molybdenum, Cadmium, Tin, Antimony, Tellurium, Barium, Mercury, Thallium, Lead and Uranium) were determined specifically for each tissue type with an ICP-MS. Certified dogfish liver (Dolt-3) was used as a quality control standard. Water quality data was provided by Rand Water and included. Statistical data were analyzed with ANOVA and post-hoc tests and parasite infection statistics were calculated. Seasonality and intensity of tapeworms were compared to the intestine and total lengths of the fish. All the tapeworms were identified as the Asian Tapeworm B. acheilognathi Yamaguti, 1934. Bothriocephalus acheilognathi attached between 10% and 20% from the anterior end of host intestine. A 100% prevalence of Asian tapeworm was recorded in all surveys and the highest mean intensity of 231.1 was recorded in autumn and the lowest mean intensity of 73.7 was recorded in summer. The fecundity of largemouth yellowfish was not influenced by the high infection rate of B. acheilognathi as young fish fry was collected during the summer survey and the fish condition was good. No correlation was observed between parasite intensity and total length of each fish. This indicates that intensity is not dependant on host size. Parasite numbers are higher and differ from a previous study. Water quality of Vaal Dam changed in summer, due to transferance of water from the Grootdraai Dam. Algal counts and secchi disk readings increased. This indicates that zooplanktonic intermediate hosts increased due to an increase in food availability and increased the chance of infection. Significant differences were recorded in one sample T-test comparisons, while ANOVA indicated that significant differences between tissue groups and seasons occurred. Bioaccumulation of metals occurred in tapeworms in Selenium, Mercury, and Lead during autumn, Copper, Zinc, Selenium, Cadmium, Antimony, Thallium and Mercury during winter, Lithium, Zinc, Selenium, Cadmium and Antimony during spring and Zinc during summer. The highest mean value was recorded in sediment, followed by water, tapeworms and then the host tissue. The general trend was sediment > water > tapeworms > liver > spinal cord > muscle. Die gebruik van intestinale parasiete as indikatore vir besoedeling het in Europa verhoog omdat die parasiete meer van die beskikbare metale as hul gashere akkumuleer. Tagtig Grootbekgeelvisse Labeobarbus kimberleyensis (Gilchrist en Thompson, 1913) was tussen April 2005 en Februarie 2006 met kieunette te RAU eiland (26° 52, 249’ S, 28 10, 249’ E) in die Vaaldam versamel. Die visse is doodgemaak, geweeg en hul lengtes bepaal. Spier, lewer en werwelkolomweefsel was versamel van elke vis en die intestinums verwyder en oopgemaak om Bothriocephalus acheilognathi bloot te lê. Die posisie van aanhegting van B. acheilognathi in die gasheer se intestinum is aangeteken. Daarna is die lintwurms in glasbottels versamel en gevries. Water en sediment was ook versamel. Geslote-flesmikrogolfvertering was gebruik om lewer, spier en lintwurms met Suprapur® Swaelsuur en werwelkolom met Suprapur® Swaelsuur en Suprapur® Soutsuur te verteer. Daarna is die metaalkonsentrasies van drie-en twintig elemente (Litium, Berillium, Titanium, Vanadium, Chroom, Mangaan, Yster, Kobalt, Nikkel, Kopper, Sink, Arseen, Selenium, Molibdenum, Cadmium, Tin, Antimoon, Tellurium, Barium, Kwik, Tallium, Lood and Uranium) spesifiek vir elke weefseltipe bepaal met ‘n IGP-MS. Gesertifiseerde haailewer (Dolt-3) was gebruik as ‘n standaard ter wille van kwaliteitskontrole. Waterkwaliteitsdata is deur Rand Water verskaf en word ingesluit. Statistiese data was geanaliseer met ANOVA en nagraadse toetse en parasiet infeksie statistieke was bereken. Seisonaliteit en intensiteit van die lintwurms was vergelyk met die totale lengtes van visse en intestinumlengte van die visse. Al die lintwurms was as Asiese lintwurms B. acheilognathi Yamaguti, 1934 geïdentifiseer. Bothriocephalus acheilognathi was tussen 10% en 20% vanaf die anteriorgedeelte van die gasheerintestinum geheg. ‘n 100% persentasiebesmetting was opgeteken in alle opnames met Asiese lintwurms en die hoogste gemiddelde besmettingsintensiteit van 231.1 was opgeteken in die herfs en laagste gemiddelde besmettingsintensiteit van 73.7 waargeneem in die somer. Die broeisukses van die Grootbekgeelvisse was nie beïnvloed deur die hoë infeksies met B. acheilognathi nie, soos blyk uit die feit dat jong vissies versamel is tydens die someropname en die viskondisie was ook goed. Daar was geen korrelasie waargeneem wanneer die parasietintensiteite en totale lengtes van die visse vergelyk is nie. Dit dui dat intensiteit nie afhanklik is van gasheergrootte nie. Die parasietgetalle is hoër en verskil van ‘n vorige studie. Die waterkwaliteit van die Vaaldam het verander gedurende somer a.g.v. die toevoer van water vanaf die Grootdraaidam. Die algtellings en die secchi-skyf lesings het dus verhoog. Dit dui dus aan dat die getal soöplanktoniese tussengashere verhoog het agv die verhoogde voedselbeskikbaarheid en ‘n hoër kans van infeksie vir visse. Betekenisvolle verskille was waargeneem wanneer enkel monster T-toets vergelykings gemaak is, terwyl ANOVA betekenisvolle verskille tussen groepe en seisoene gewys het. Bioakkumulasie van metale in lintwurms het in selenium, kwik en lood gedurende herfs, en in koper, sink, selenium, cadmium, antimoon, tallium en kwik in winter, en in litium, sink, selenium, cadmium en antimoon in lente en in sink gedurende somer voorgekom. Die hoogste gemiddelde waarde was waargeneem in sediment, gevolg deur water, lintwurms en gasheerweefsel. Die algehele akkumulasie verwantskap is sediment > water > lintwurms > lewer > werwelkolom > spier. / Prof. Annemarie Avenant-Oldewage

No description available.

The order Diphyllobothriidea is a unique group of tapeworms in terms of the wide spectrum of definitive hosts involving all groups of tetrapods including man. They are almost globally distributed in both freshwater and marine ecosystems, but there are also terrestrial species. It is very difficult to identify species of diphyllobothriid cestodes to the species level. Morphology of the eggs might represent a new method in the differential diagnosis of these parasites. Besides the size and shape of the eggs, the structure of egg surface might play a significant role in the determination of different taxa. For the analysis of these characteristics Scanning Electron Microscopy (SEM) was used. Experimental infections in hamsters and man were done.

A taxonomic revision of the bothriocephalid tapeworms from the lizardfishes of the genus Saurida, based on the morphology and biometry, was carried out. A new material from four species of lizardfishes from Indonesia was examined and compared with literature and available type material. Results indicate only 2 species valid (Oncodiscus sauridae Yamaguti, 1934; Penetrocephalus ganapattii (Rao, 1954) Rao 1960).

Morphological-taxonomical study of gryporhynchid cestodes from Africa is presented. New material from Kenya, Senegal and Sudan was studied and we belong to 17 species in 8 orders - Amirthalingamia macracantha (Joyeux et Baer, 1935) Bray, 1974; Anomotaenia riccii (Fuhrmann et Baer, 1943) Baer et Bona, 1960; Cyclustera magna (Baer, 1959) Bona, 1975; Cyclustera sp.; Dendrouterina macrosphincter (Fuhrmann, 1909) Baer et Bona, 1960; Neogryporhynchus sp.; Paradilepis lloydi (Southwell, 1926) Spasski, 1954; Paradilepis maleki (Khalil, 1961); Paradilepis urceina Bona, 1975; Paradilepis urceus (Wedl, 1855) Joyeux et Baer, 1950; Parvitaenia macrocoleo Bona, 1975; Parvitaenia macropeos (Wedl, 1855) Baer et Bona, 1960; Parvitaenia megascolecina Bona, 1975; Parvitaenia purpurea Johri, 1959; Parvitaenia samfyia Mettrick, 1967; Valipora ardeolae (Singh, 1952) Baer et Bona, 1960 and Valipora mutabilis (Linton, 1927). Known larval stages and adults from Africa are presented in review of species.