Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-25T23:29:05Z No. of bitstreams: 1 AndersonRodriguesDeOliveira_DISSERT.pdf: 2673961 bytes, checksum: 94e48d2aac9ebb6eb6d30bb575363364 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-08-04T22:39:37Z (GMT) No. of bitstreams: 1 AndersonRodriguesDeOliveira_DISSERT.pdf: 2673961 bytes, checksum: 94e48d2aac9ebb6eb6d30bb575363364 (MD5) / Made available in DSpace on 2016-08-04T22:39:37Z (GMT). No. of bitstreams: 1 AndersonRodriguesDeOliveira_DISSERT.pdf: 2673961 bytes, checksum: 94e48d2aac9ebb6eb6d30bb575363364 (MD5) Previous issue date: 2016-03-18 / Diabetes Mellitus (DM) ? um grupo de doen?as caracterizada por anormalidades metab?licas e complica??es cr?nicas. A hiperglicemia caracter?stica dessa doen?a produz glicosila??o de prote?nas e consequente ac?mulo de produtos finais da glicosila??o em v?rios tecidos humanos, entre eles, o tend?o. O exerc?cio aer?bio (EA) e a terapia laser de baixa intensidade (TLBI) t?m sido utilizados no manejo de tendinopatias em indiv?duos com ou sem DM. Objetivo: O objetivo desse estudo ? observar o efeito da TLBI e o EA, associados, no processo de reparo tecidual do tend?o de Aquiles (TA) de ratos diab?ticos. M?todos: Foram utilizados 91 animais divididos nos seguintes grupos: grupo controle (GC), grupo controle lesionado (GCL), grupo diab?tico (GD), grupo diab?tido laser (GDL), grupo diab?tico treinado (GDT) e grupo diab?tico treinado laser (GDTL). De acordo com o grupo, os ratos foram submetidos interven??o do EA, TLBI ou associa??o dos dois recursos. Foram analisadas as caracter?sticas morfol?gicas, biomec?nicas e moleculares. Resultados: O peso final dos animais diab?ticos foi estatisticamente menor quando comparado com os animiais controles (p <0,01). A glicemia dos animais diab?ticos foi estatisticamente maior quando comparado aos grupos controles (p<0,01). Os testes biomec?nicos indicaram melhoras significativas nos animais dos grupos GC e GDTL quando comparados aos grupos diab?ticos nas vari?veis de carga m?xima, distens?o, energia absorvida, stress, ?rea de sec??o transversa, m?dulo el?stico e densidade de energia (p<0,05). A an?lise por meio de biologia molecular indicou que a associa??o do exerc?cio aer?bio e Laser promoveu aumento da express?o g?nica de col?geno I e modulou a express?o das MMP2 e MMP9. N?o foi observada melhora significativa nas vari?veis morfol?gicas estudadas. Conclus?o: a TLBI associada ao exerc?cio aer?bio promove melhora nas propriedades mec?nicas e no controle da express?o g?nica do col?geno I e da MMP2 e MMP9 de ratos diab?ticos. Palavras-chave Diabetes Mellitus. Fototerapia. Exerc?cio. Tend?o de Aquiles. Cicatriza??o / Diabetes Mellitus (DM ) is a complex disease that requires continuous medical care for the reduction of risk factors in addition to glycemic control. The typical hyperglycemia of this disease produces glycosylation of proteins and so the consequence is the accumulation of glycosylation final products in various human tissues, among them, the tendon. The aerobic exercise (AE) and the low level laser therapy (LLLT) have been used to treat tendinopathies in individuals with or without DM. Objective: The aim of this study was to watch the effect of the LLLT and the AE, in association, in partial tenotomy of the tissue repair of the Achilles tendon (AT) of diabetic rats. Methods: 91 animals were utilized and divided in to the following groups: control group (GC), injured control group (GCL), diabetic group (GD), diabetic group LLLT (GD ? TLBI), diabetic group trained (GD - EX) and diabetic group trained laser (GD-EX+TLBI). The animals were submitted to intervention with AE, using a protocol with a progressive increase of time (12 to 60 min) and speed of (4 to 9 m/min), and the LLLT (660 nm laser, 10mW, 4 J/cm?, single point for 16 seconds, three times for week). It was analyzed morphological, biomechanical and molecular characteristics. For data showing normal distribution was used one-way ANOVA test and post hoc Tukey and data without normal distribution was used Mann Whitney test and post hoc Dunn's. It was accepted p <0.05 for statistical significance Results: The biomechanical tests indicated major improvement in the GC and GD-EX+TLBI groups when compared with the diabetic groups in the following variables: maximum load, strain, absorbed energy, stress, cross section area, elastic modulus and energy density (p<0.05). The analysis through molecular biology indicated that the association of aerobic exercise and LLLT generated an increase of the collagen I gene expression and modulated the expression of the MMP2 and MMP9 (p<0.05). No observed any major improvement in the morphological variable studied. Conclusion: the LLLT associated with aerobic exercise promotes and increase of the mechanical properties, in the control of collagen I gene expression and of the MMP2 and MMP9 of the diabetic rats.