by Pau Hon-Man. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 146-179). / Abstracts in English and Chinese. / ACKNOWLEDGEMENT --- p.i / ABBREVIATIONS --- p.iii / ABSTRACT (English) --- p.v / ABSTRACT (Chinese) --- p.ix / FLOW CHART --- p.xi / INDEX OF FIGURES --- p.xxi / INDEX OF TABLES --- p.xxiv / PUBLICATION LIST --- p.xxv / Chapter CHAPTER 1 --- INTRODUCTION --- p.1 / Chapter 1.1 --- Basic Structure of Tendons --- p.3 / Chapter 1.2 --- Anatomy of Patellar Tendon --- p.9 / Chapter 1.3 --- Cellular Characteristics of Fibroblasts --- p.11 / Chapter 1.4 --- Healing Process in Tendons --- p.13 / Chapter 1.5 --- Chronic Tendon Disorder --- p.19 / Chapter 1.6 --- Aims and Objectives --- p.25 / Chapter 1.7 --- Study Plan --- p.26 / Chapter CHAPRER 2 --- MATERIALS AND METHODOLOGY --- p.35 / Chapter 2.1 --- Collection of human Tissues Samples --- p.38 / Chapter 2.2 --- Tissue Culture --- p.40 / Chapter 2.3 --- Preparation of Conditioned Medium --- p.46 / Chapter 2.4 --- Proliferation Response of Tendon Fibroblasts --- p.48 / Chapter 2.5 --- Invasion Assay --- p.53 / Chapter 2.6 --- Chick Chorioallantonic Membrane Model --- p.56 / Chapter 2.7 --- SDS-PAGE --- p.58 / Chapter 2.8 --- Enzyme Linked Immunosorbent Assay --- p.63 / Chapter 2.9 --- Immunocytochemical Staining --- p.68 / Chapter 2.10 --- Statistical Analysis --- p.69 / Chapter CHAPTER 3 --- RESULTS --- p.74 / Chapter 3.1 --- Patient's Information --- p.76 / Chapter 3.2 --- Primary Explant Cell Culture --- p.77 / Chapter 3.3 --- Proliferation Response of Tendon Fibroblasts --- p.79 / Chapter 3.4 --- Invasion Assay --- p.82 / Chapter 3.5 --- Chick Chorioallantonic Membrane Model --- p.83 / Chapter 3.6 --- SDS-PAGE --- p.85 / Chapter 3.7 --- Enzyme Linked Immunosorbent Assay --- p.86 / Chapter 3.8 --- Immunocytochemical Staining --- p.88 / Chapter CHAPTER 4 --- DISCUSSION --- p.116 / Chapter 4.1 --- Higher Proliferation Capacity of Tendinosis Fibroblasts --- p.118 / Chapter 4.2 --- Tendinosis Fibroblasts Secrete Angiogenesis Factors to Enhance Angiogenesis --- p.123 / Chapter 4.3 --- Tendinosis Fibroblasts Secrete More Proteolytic Enzyme --- p.126 / Chapter 4.4 --- Proinflammatory Characteristics in Tendinosis Fibroblasts --- p.129 / Chapter 4.5 --- Comparison of in vitro and Histological Study --- p.132 / Chapter 4.6 --- Tendinosis Fibroblasts Seems to be Transformed --- p.134 / Chapter 4.7 --- Limitation of Study --- p.136 / Chapter CHAPTER 5 --- FUTURE RESEARCH DIRECTION --- p.140 / Chapter 5.1 --- Further Exploration of the Factors Secreted in Tendinosis Fibroblasts --- p.141 / Chapter 5.2 --- Proteoglycan Synthesis in Tendinosis Fibroblasts --- p.142 / Chapter 5.3 --- Tendinosis Animal Model and Clinical Study --- p.143 / Chapter CHAPTER 6 --- CONCLUSION --- p.144 / BIBLIOGRAPHY --- p.146 / APPENDIX --- p.180

by Wang Wen. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 113-124). / Abstracts in English and Chinese. / ABSTRACT --- p.i / FLOWCHART --- p.vi / ACKNOWLEDGEMENT --- p.x / ABBREVIATIONS --- p.xi / INDEX FOR FIGURES --- p.xii / INDEX FOR TABLES --- p.xv / TABLE OF CONTENTS --- p.xvi / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 1.1 --- PATELLAR TENDINOSIS --- p.1 / Chapter 1.1.1 --- Introduction --- p.1 / Chapter 1.1.2 --- Epidemiology of Patellar Tendinosis --- p.3 / Chapter 1.1.3 --- Etiology of Patellar Tendinosis --- p.3 / Chapter 1.1.4 --- Manifestations of Patellar Tendinosis --- p.4 / Chapter 1.1.5 --- Imaging Examination on Patellar Tendinosis --- p.4 / Chapter 1.1.6 --- Clinical Diagnosis of Patellar Tendinosis --- p.6 / Chapter 1.1.7 --- Management of Patellar Tendinosis … --- p.6 / Chapter 1.2 --- ANATOMY AND HISTOLOGY OF PATELLAR TCNDON --- p.7 / Chapter 1.3 --- STRUCTURE AND METABOLISM OF TENDON --- p.9 / Chapter 1.3.1 --- Tenocytes --- p.9 / Chapter 1.3.2 --- Extra-cellular Matrix --- p.11 / Chapter 1.3.2.1 --- Collagen --- p.11 / Chapter 1.3.2.2 --- Proteoglycans --- p.12 / Chapter 1.4 --- ROLES OF GROWTH FACTORS TENDON HEALING AND REPAIR --- p.14 / Chapter 1.4.1 --- Platelet-Derived Growth Factor --- p.14 / Chapter 1.4.2 --- Transforming Growth Factor-beta --- p.15 / Chapter 1.5 --- HISTOPATHOLOGY OF PATELLAR TENDINOSIS --- p.16 / Chapter 1.6 --- STUDY PLAN --- p.17 / Chapter 1.6.1 --- Characterization on Hypercellularity --- p.18 / Chapter 1.6.2 --- Characterization on Disorganization and Loosening of Collagen --- p.18 / Chapter 1.6.3 --- Characterization on Inflammatory Trace --- p.20 / Chapter 1.6.4 --- Characterization on Growth Factors in Tendinosis --- p.21 / Chapter 1.7 --- OBJECTIVES --- p.22 / Chapter 2. --- MATERIALS AND METHODS --- p.27 / Chapter 2.1 --- HUMAN TISSUES --- p.27 / Chapter 2.1.1 --- Patellar Tendinosis Tissues --- p.27 / Chapter 2.1.1.1 --- Diagnosis of patellar tendinosis --- p.27 / Chapter 2.1.1.2 --- Recruitment of patients --- p.27 / Chapter 2.1.4 --- Healthy Patellar Tendon tissues --- p.28 / Chapter 2.2 --- TISSUES COLLECTION AND PREPARATION --- p.28 / Chapter 2.3 --- HISTOLOGICAL STUDY ON HUMAN SPECIMENS --- p.28 / Chapter 2.3.1 --- Haematoxyline and Eosin Staining --- p.29 / Chapter 2.3.2 --- Safranin O Staining --- p.29 / Chapter 2.3.2.1 --- Reagents preparation --- p.29 / Chapter 2.3.2.2 --- Experimental procedure --- p.30 / Chapter 2.3.5 --- Polarization Microscopy --- p.30 / Chapter 2.4 --- IMMUNOHISTOCHEMICAL STAINING --- p.30 / Chapter 2.4.1 --- Reagents Preparation --- p.31 / Chapter 2.4.2 --- Experimental Procedure --- p.33 / Chapter 2.5 --- IMAGE ANALYSIS --- p.35 / Chapter 2.5.1 --- Equipment --- p.35 / Chapter 2.5.2 --- Procedures --- p.35 / Chapter 2.6 --- IN SITU ZYMOGRAPHY --- p.37 / Chapter 2.6.1 --- Reagents Preparation --- p.37 / Chapter 2.6.2 --- Experimental Procedure --- p.38 / Chapter 2.7 --- STATISTIC ANALYSIS.… --- p.39 / Chapter 3. --- RESULTS --- p.42 / Chapter 3.1 --- HUMAN SAMPLES --- p.42 / Chapter 3.1.1 --- Patellar tendinosis patients --- p.42 / Chapter 3.1.2 --- Healthy control group --- p.43 / Chapter 3.2 --- HISTOLOGICAL STUDY ON HUMAN SPECIMENS --- p.43 / Chapter 3.2.1 --- Gross Morphology --- p.43 / Chapter 3.2.2 --- Haematoxyline and Eosin Staining --- p.44 / Chapter 3.2.3 --- Safranin O Staining --- p.44 / Chapter 3.2.4 --- Polarization Microscopy --- p.44 / Chapter 3.3 --- IMAGE ANALYSIS --- p.45 / Chapter 3.3.1 --- Immunohistochemistry of PCNA --- p.45 / Chapter 3.3.2 --- Immunohistochemistry of hsp47 --- p.46 / Chapter 3.3.3 --- Immunohistochemistry of Procollogen Type I --- p.47 / Chapter 3.3.4 --- Immunohistochemistry of MMP1 --- p.47 / Chapter 3.3.5 --- Immunohistochemistry of TIMP1 --- p.48 / Chapter 3.3.6 --- Immunohistochemistry of COX-2 --- p.49 / Chapter 3.3.7 --- Immunohistochemistry of TGFP --- p.49 / Chapter 3.3.8 --- Immunohistochemistry of PDGFbb --- p.50 / Chapter 3.3.9 --- Immunohistochemistry of PDGFRβ --- p.51 / Chapter 3.3.10 --- Summary of Image Analysis of Immunohistochemical staining --- p.51 / Chapter 3.4 --- IN SITU ZYMOGRAPHY --- p.52 / Chapter 4. --- DISCUSSION --- p.93 / Chapter 4.1 --- DIAGNOSIS OF PATELLAR TENDINOSIS --- p.93 / Chapter 4.2 --- HYPERCELLULARITY IN PATELLAR TENDINOSIS --- p.95 / Chapter 4.3 --- COLLAGEN DISTURBANCE IN PATELLAR --- p.97 / Chapter 4.4 --- INFLAMMATORY RESPONSE IN PATELLAR TENDINOSIS --- p.100 / Chapter 4.5 --- THE EXPRESSION OF GROWTH FACTORS IN PATELLAR TENDINOSIS --- p.102 / Chapter 4.6 --- PROPOSED PATHOGENESIS FOR PATELLAR TENDINOSIS --- p.105 / Chapter 4.7 --- LIMITATION OF THIS STUDY --- p.108 / Chapter 4.8 --- FUTURE STUDY --- p.109 / Chapter 5. --- CONCLUSION --- p.111 / BIBLIOGRAPHY --- p.113