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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

In vivo acute changes in ATP and glucose concentrations in the rat testicles following unilateral torsion: experimental study / Estudo experimental das alteraÃÃes agudas na glicemia e concentraÃÃes testiculares de ATP e glicose induzidas por torÃÃo unilateral, in vivo

SÃrgio Botelho GuimarÃes 04 April 2002 (has links)
CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / Spematic cord torsion is one of the more common surgical urological emergencies in childhood. An experimental study has been conducted to investigate acute changes in testicular concentrations of ATP and glucose following one-hour unilateral 720 testicular torsion. Sixty prepubertal male Wistar rats were distributed into two groups: Group 2(Ischemia) and Group 1(Sham operated). Each group(n=30) was divided into 5 subgroups (n=6). All animals were subjected to right testicular torsion of 720Â. The testicular torsion of animals from Group 1 was immediately corrected. Group 2 animals had their testicular torsion lasting one hour followed by detorsion. Testicles were collected and arterial blood samples taken from abdominal aorta of each animal subgroup at the and of ischemic period or one hour after sham operation and 0.5, 1,6 and 24 hours later. ATP and glucose concentrations were significantly decreased (p<0,05) in ischemia group as compared to sham group at the end of ischemic period. There was no difference in glycemia when comparing ischemic versus sham-operated rats. Glucose concentrations were significantly increase (p<0,05) in ipsilateral testis of sham-operated rats No significant change in ATP and glucose concentrations were found in the left testicle in comparasion to the ones measured in the rigth testicle of the same animal. The decrease in ATP and glucose concentrations may be explained by the reduction in blood flow to the torted testis imposed by torsion. The absence of significant contralateral alterations in the concentrations of ATP and glucose could be related to the limited time of isquemia imposed to the right testis. It is concluded that unilateral one-hour testicular torsion causes a decrease in ipsilateral testicular perfusion resulting in decresead testicular concentrations of glucose and ATP in the ipstelial (right) testicles and no alterations in the concentrations of glucose and ATP in the contraleral testicles. Simple testicle manipulation (Sham Operation) causes a signficant increase in glucose concentrations in the ipsilateral(right)testis. / A torÃÃo do cordÃo espermÃtico à a emergÃncia geniturinÃria mais comum na idade pediÃtrica. Foram estudadas as alteraÃÃes metabÃlicas agudas nas concentraÃÃes testiculares de ATP e glicose apÃs uma hora de isquemia induzida por torÃÃo do cordÃo espermÃtico. Sessenta ratos wistar machos prÃ-pÃberes, foram distribuÃdos em 2 grupos de 30 animais cada e cada grupo em 5 subgrupos de 6 ratos. Os ratos do grupo 1 foram submetidos à torÃÃo seguida por distorÃÃo imediata (cirurgia simulada) do testÃculo direito. Os animais do grupo 2 foram submetidos à torÃÃo do testÃculo direito (720o) durante uma hora. Os animais dos diferentes subgrupos foram sacrificados 30 minutos, 1 hora, 6 horas e 24 horas apÃs a distorÃÃo ou realizaÃÃo da cirurgia simulada para a remoÃÃo dos testÃculos e coleta de amostra de sangue arterial. As alteraÃÃes das concentraÃÃes de glicose e ATP foram determinadas por mÃtodos enzimÃticos. Ocorreu queda significante (p<0,05) da concentraÃÃo de glicose e ATP no tempo 0 h, nos testiculos isquemiados. NÃo ocorreram alteraÃÃes na glicemia nem nas concentraÃÃes de glicose e ATP nos testÃculos contralaterais. Os animais submetidos à cirurgia simulada apresentaram aumento significante (p< 0,05) da concentraÃÃo de glicose no testÃculo ipsilateral quando comparada à concentraÃÃo de glicose no testÃculo esquerdo dos mesmos animais nos tempos 0 h e 24h. A diminuiÃÃo do fluxo sangÃÃneo em decorrÃncia da torÃÃo explica a reduÃÃo das concentraÃÃes de ATP e glicose nos testÃculos isquemiados. A ausÃncia de alteraÃÃes nas concentraÃÃes de ATP e glicose nos testÃculos contralaterais (esquerdos) poderia ser uma decorrÃncia do reduzido tempo de isquemia imposto ao testÃculo ipsilateral (direito). Concluiu-se que a torÃÃo testicular unilateral de 720o, por uma hora, induz uma reduÃÃo significante (p < 0,05) das concentraÃÃes testiculares de ATP e de glicose no testÃculo isquemiado sem alteraÃÃes correspondentes da glicemia. A simples manipulaÃÃo do testÃculo direito (cirurgia simulada) induz um aumento da concentraÃÃo de glicose testicular.

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