Th.--Pharm.--Montpellier 1, 1978.

The aim of this research was to investigate the phytochemistry of two species Sanicula europaea and Teucrium davaeanum which are traditionally used in treatment of wounds. Four compounds were isolated from the 80% methanolic extract of S. europaea; bis-(2-ethylhexyl) phthalate (1), palmitic acid (2), rosmarinic acid (3), saniculoside N (4). Compounds 1 and 2 were isolated for the first time from this species. The structure elucidation of the isolated compounds was on the basis of 1D, 2D NMR spectroscopy and mass spectrometry measurements. Two compounds were isolated from the crude glycosides extract of T.davaeanum; 6 is a phenylethanoid glycoside and 8 is an iridoid glycoside, from the data available these may be new compounds for which the names davaeanuside A and davaeanuside B are proposed respectively." The total polyphenol content of S. europaea L, T. davaeanum leaves-flowers and T. davaeanum stem were found to be 5.0, 1.20 and 0.65 mg per 100 mg dried plant material respectively. A study of the antioxidant activity of the 50 % ethanol extracts of S. europaea and T. davaeanum showed that on a mg/mg basis S. europaea and T. davaeanum have approximately 5%, 8 % antioxidant capacity of Trolox respectively. A study of the cytotoxic activity of davaeanuside A (6), iridoid glycoside (7), davaeanuside B (8) and saponin compound (10) isolated from the crude glycosides extract of T. davaeanum revealed that saponin compound (10) inhibited the growth of Hela cells by 50 % at 50 μg/ml, P< 0.001, but the other compounds did not show activities against the tested cell lines at 100 μg/ml. The results of this work provide some basis for the traditional use of these species in the treatment of wounds. / Ministry of high education in Libya

The aim of this research was to investigate the phytochemistry of two species Sanicula europaea and Teucrium davaeanum which are traditionally used in treatment of wounds. Four compounds were isolated from the 80% methanolic extract of S. europaea; bis-(2-ethylhexyl) phthalate (1), palmitic acid (2), rosmarinic acid (3), saniculoside N (4). Compounds 1 and 2 were isolated for the first time from this species. The structure elucidation of the isolated compounds was on the basis of 1D, 2D NMR spectroscopy and mass spectrometry measurements. Two compounds were isolated from the crude glycosides extract of T.davaeanum; 6 is a phenylethanoid glycoside and 8 is an iridoid glycoside, from the data available these may be new compounds for which the names davaeanuside A and davaeanuside B are proposed respectively." The total polyphenol content of S. europaea L, T. davaeanum leaves-flowers and T. davaeanum stem were found to be 5.0, 1.20 and 0.65 mg per 100 mg dried plant material respectively. A study of the antioxidant activity of the 50 % ethanol extracts of S. europaea and T. davaeanum showed that on a mg/mg basis S. europaea and T. davaeanum have approximately 5%, 8 % antioxidant capacity of Trolox respectively. A study of the cytotoxic activity of davaeanuside A (6), iridoid glycoside (7), davaeanuside B (8) and saponin compound (10) isolated from the crude glycosides extract of T. davaeanum revealed that saponin compound (10) inhibited the growth of Hela cells by 50 % at 50 μg/ml, P< 0.001, but the other compounds did not show activities against the tested cell lines at 100 μg/ml. The results of this work provide some basis for the traditional use of these species in the treatment of wounds.

La richesse de la flore de Corse et de l'ouest algérien n‟est plus à démontrer. Le bassin méditerranéen est l'un des " points chauds " ou hotspot dans lesquels se trouve concentrée la biodiversité. A l'instar des autres zones rouges régionales, ces deux régions sont caractérisées par une richesse spécifique, un taux d‟endémisme élevé mais aussi par des menaces anthropiques fortes et en augmentation rapide. Les huiles essentielles sont des produits à forte valeur ajoutée utilisées dans des domaines aussi divers que la parfumerie, les cosmétiques, l‟agro-alimentaire ou encore l'aromathérapie et la pharmacie. Elles se présentent sous forme de mélanges complexes de plusieurs dizaines voire de plusieurs centaines de composés en général terpéniques présents dans des proportions variables. La production et la caractérisation des huiles essentielles, le contrôle de leur qualité tout autant que la mise en évidence d'une éventuelle spécificité nécessite la mise en oeuvre des méthodes de préparation et d'analyses les plus modernes. Ce travail de thèse repose sur l‟étude des huiles essentielles et des fractions volatiles de 9 espèces et sous-espèces du genre Teucrium, poussant en Corse et à l‟ouest algérien.Notre objectif est de contribuer à l‟amélioration des connaissances des ressources naturelles issues de la biomasse végétale en fournissant des informations scientifiques objectives. Ce travail de thèse a été l‟occasion de développer un travail méthodologique et appliqué totalement complémentaire. Ainsi trois grands volets ont été développés : Le principal volet est chimique, il concerne la caractérisation des compositions chimiques des huiles essentielles, les deux autres volets sont totalement complémentaires : (i) un volet génétique dont le but est d‟établir les séquences génétiques des plantes étudiées et examiner l‟impact du patrimoine génétique sur la production de métabolites secondaires (ii) un volet microbiologique basé sur la mise en évidence d‟activités antimicrobiennes des huiles essentielles étudiées.