Thesis (M. Med. Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 25-26).

Thesis (M.Med.Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 25-26). Also available in print.

Thesis (M. Med. Sc.)--University of Hong Kong, 2008. / Includes bibliographical references (leaf 90-93)

Thesis (M.D.)--University of Hong Kong, 1998. / Includes bibliographical references.

Thesis (M. Phil.)--University of Hong Kong, 1998. / Includes bibliographical references (leaves 152-162).

Thesis (M.D.)--University of Hong Kong, 1998. / Includes bibliographical references. Also available in print.

No description available.

by Senok, Abiola Catherine. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 206-236). / ACKNOWLEDGEMENT --- p.ii / LIST OF ABSTRACTS AND PRESENTATIONS --- p.iv / ABSTRACT --- p.viii / INTRODUCTION AND OBJECTIVES --- p.1 / Chapter SECTION ONE : --- LITERATURE REVIEW --- p.8 / Chapter CHAPTER 1 : --- THE MALARIA PARASITE --- p.8 / Chapter CHAPTER 2 : --- RED CELL PHYSIOLOGY --- p.19 / Chapter CHAPTER 3 : --- THE THALASSAEMIAS --- p.27 / Chapter CHAPTER 4 : --- PROTECTION AGAINST MALARIA IN THALASSAEMIA: THE EVIDENCE --- p.32 / Chapter CHAPTER 5 : --- POSSIBLE MECHANISMS INVOLVED IN THE PROTECTION AGAINST MALARIA IN THALASSAEMIC RBC --- p.40 / Chapter SECTION TWO : --- RED CELL FRACTIONATION --- p.48 / Chapter CHAPTER 6 : --- COMPARISON OF FRACTIONATION TECHNIQUES --- p.48 / Chapter CHAPTER 7 : --- PERCOLL SEPARATION OF THALASSAEMIC RBC --- p.63 / Chapter SECTION THREE : --- INHIBITION OF P. FALCIPARUM ACTIVITY IN THALASSAEMIC RBC --- p.79 / Chapter CHAPTER 8 : --- IN VITRO CULTURE OF P. FALCIPARUM --- p.79 / Chapter CHAPTER 9 : --- PARASITE GROWTH IN THALASSAEMIC RBC --- p.92 / Chapter SECTION FOUR : --- OXIDATIVE STRESS AS A PROTECTIVE MECHANISM --- p.127 / Chapter CHAPTER 10 : --- ASSESMENT OF SENSITIVITY TO OXIDATIVE STRESS --- p.127 / Chapter CHAPTER 11 : --- EFFECT OF OXIDANT MODULATING AGENTS ON PARASITE ACTIVITY --- p.143 / Chapter SECTION FIVE : --- ULTRASTRUCTURE OF MALARIA PARASITES IN THALASSAEMIC RBC --- p.180 / Chapter CHAPTER12 --- p.180 / Chapter SECTION SIX : --- FUTURE WORK AND CONCLUSION --- p.202 / Chapter CHAPTER13 --- p.202 / REFERENCES --- p.205

Thesis (M. Med. Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 62-65).

α-thalassemia is an inherited globin gene disorder commonly found among the Chinese population. It is composed of both non-deletional and deletional α-globin gene mutations. Classical α-thalassemia presents with red cell microcytosis but silent cases with a normal mean corpuscular volume (MCV) are also seen. Routine laboratory testing methods for large-scale detection of silent α-thalassemia mutations are onerous and time-consuming. Furthermore, methods such as denaturing high performance liquid chromatography (HPLC) or denaturing gradient gel electrophoresis (DGGE) for scanning of point mutations are costly and they require post-PCR separation. High resolution melting (HRM) analysis is an economical, sensitive, and fast method for large scale point mutation scanning. Contamination is significantly reduced with HRM because the process is performed in a closed-tube environment and does not require post-PCR manipulation. We used HRM and multiplex gap-PCR analysis to determine the prevalence of silent α-thalassemia carriers in Hong Kong. Of the 223 hematologically normal blood samples scanned by Roche LightCycler 480®, HRM did not show any sample with a non-deletional α-globin gene mutation of clinical significance. α-multiplex gap-PCR analysis revealed 36 samples (16.1%) with single α-globin gene deletions. The detection of single α-globin gene deletions in samples with a MCV greater than 80 fL indicates that the previously reported prevalence of α-thalassemia mutations in our Chinese population based on MCV screening is under-estimated. The data also suggest that non-deletional α-thalassemia mutations presenting with a normal MCV are very rare, and they most likely present with microcytosis. The fact that most silent α-thalassemia mutations are due to large deletions supports the use of traditional molecular techniques such as gap-PCR for their detection. HRM can be used as an adjunct tool for large-scale population screening of non-deletional mutations. This study provides more accurate data on the prevalence of silent α-thalassemia carriers in the Hong Kong Chinese population. The information will facilitate genetic counseling and risk assessment in families carrying α-thalassemia mutations. / published_or_final_version / Pathology / Master / Master of Medical Sciences