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Det var en gång... : Halta-Cajsa berättade, en analys av småländska sägner / Once Upon a Time... : Halta-Cajsa told, an Analysis of Legends from the Region of SmålandSvensson, Patrik January 2012 (has links)
This essay is intended to illustrate the norms and values which can be deduced from the notifier Catarina Andersdotter and recorded by Gunnar-Olof Hyltén-Cavallius. Reflects the legends of the peasant culture and life during the later part of 1700´s and early 1800´s. In the background material, I have assumed the following conditions of life; faith, love, work and children. Since Hyltén-Cavallius is our filter between us and the notifier, I have examined his life and incorporated events that may be of interest of the essay. Catarina Andersdotter´s life is described in the essay to show the factors and living conditions in her life that influenced her choice of stories. The environment of the commons over time period is described for the purpose of the variables of the legends. Legends have been qualitatively and quantitatively investigated. The essay shows that most of the legends deal with mytical world and its creatures as well as the interaction with humans. People are described mainly in situation dealing with everyday task of the country. The essay shows the connection between folklore and Christian world view. To obtain deeper and more adequate pictures of the values and norms among the commons during the time period a wide range of sources need to be used.
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Eine Instant-Messenger-Infrastruktur für die TU ChemnitzPetersen, Karsten 27 April 2004 (has links) (PDF)
Workshop "Netz- und Service-Infrastrukturen"
Der Vortrag beleuchtete den Gedanken einer Instant-Messenger Infrastruktur für die Uni und den sich daraus ergebenden Möglichkeiten, Problemen sowie offenen Fragen.
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Demonstration of specific physical interaction between CHOP mRNA and intracellular proteinsChan, Yin-tung, Crystal., 陳燕彤. January 2011 (has links)
The ability of a cell to respond precisely to environmental stress depends on the expression of a large number of genes in a finely coordinated manner. One of such genes is CHOP that encodes the CCAAT/Enhancer-Binding Protein Homologous Protein. CHOP is usually expressed to mediate apoptosis under the condition of excessive stress. The expression of CHOP therefore has to be stringently regulated as its expression will determine the fate of a cell under stress. The expression of many genes is regulated at the posttranscriptional level through the metabolism of their mRNA, such as maturation, transport, storage, and degradation of mRNA. Many metabolic processes of mRNA are known to be mediated by RNA-binding proteins that specifically interact with the mRNA. RNA-binding proteins that interact with the CHOP mRNA have until present not been identified. The aim of this study is to investigate what proteins may bind specifically to CHOP mRNA. The study will enable further understanding regarding how the expression of CHOP is regulated in cellular stress response.
Proteins extracted from HeLa cells were incubated with a 335bp [3H]-labelled CHOP RNA probe that spans over a part of the coding region and the 3’UTR of CHOP mRNA. Sucrose density gradient ultracentrifugation revealed that after incubation with proteins extracted from HeLa cells, the sedimentation rate of the [3H]-CHOP RNA probe was significantly higher than that of the free [3H]-RNA probe. The formation of heavy molecular complexes involving the [3H]-CHOP RNA probe was therefore suggested. However, no increase in sedimentation rate of the [3H]-CHOP RNA probe was observed in the presence of an excess of unlabelled CHOP RNA probe. Similar observations were made when the experiments were performed using proteins isolated from cells treated with As2O3.
Two putative sequence elements, the Adenylate-Uridylate-Rich Element (ARE) and the Putative Regulatory Element (PRE) located respectively in the 3’UTR and coding region of the CHOP mRNA were then examined for their involvement in RNA-protein interaction. The deletion of ARE and/or PRE, from the [3H]-CHOP RNA probe had little effect on the binding of the RNA probe to the HeLa cell proteins. Consistently, unlabelled CHOP RNA probes with the same deletions were only slightly weaker in competing with the intact [3H]-CHOP RNA probe to bind to HeLa cell proteins. Human Antigen R (HuR) was identified by Western blot analysis to be present in the proteins that were obtained by pull-down assays using biotinylated CHOP RNA as a probe. The deletion of ARE and/or PRE resulted in a slight reduction of HuR obtained by pull down assays.
This study provides the first evidence that physical binding interaction occurs between intracellular RNA-binding proteins and CHOP mRNA. More importantly, one such protein is HuR. Data suggest that HuR binding to the CHOP mRNA is mediated by sequences in the CHOP mRNA other than ARE and PRE. / published_or_final_version / Biochemistry / Master / Master of Philosophy
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Characterization of the roles of yeast nuclear exosome cofactor TRAMP complex in pre-mRNA splicingKong, Ka-yiu, 江家耀 January 2013 (has links)
In budding yeast, the Trf4/5p-Air1/2p-Mtr4p polyadenylation (TRAMP) complex recognizes unwanted RNA transcripts in the nucleus and then targets them to the nuclear exosome for rapid degradation, constituting an important pathway of nuclear RNA quality control. Each pre-mRNA splicing event unavoidably generates a RNA side-product that should be recognized by TRAMP and then removed by the nuclear exosome to prevent the potentially harmful sequestration of splicing factors and/or ribonucleotides. While successful pre-mRNA splicing inevitably produces a spliced-out intron, errors in pre-mRNA splicing lead to the emergence of either an abnormal splicing intermediate, or a splicing-incompetent pre-mRNA that cannot be properly spliced. However, it remains unclear how and when these RNA side-products of pre-mRNA splicing are recognized by TRAMP. In this study, chromatin immunoprecipitation (ChIP) was applied to demonstrate that both TRAMP and the nuclear exosome component Rrp6p are cotranscriptionally recruited to nascent RNA transcripts, particularly to intronic sequences, indicating that splicing side-products are recognized by TRAMP and committed to subsequent nuclear-exosome-mediated degradation in a cotranscriptional manner. Deletion of TRF4, of both AIR1 and AIR2, or of RRP6, resulted in accumulation of unspliced pre-mRNAs. Surprisingly, while such pre-mRNAs accumulated in rrp6 cells owing to defects in pre-mRNA degradation, the same phenotype in trf4 and air1air2 cells involved splicing defects, demonstrating that only TRAMP, but not the nuclear exosome, contributes to optimal pre-mRNA splicing. Consistent with a direct stimulatory role for TRAMP in pre-mRNA splicing, negative genetic interactions and physical interactions between Trf4p and several splicing factors were observed, and that Trf4p was further shown to be required for optimal recruitment of the splicing factor Msl5p. The direct facilitation of pre-mRNA splicing by TRAMP may act as a fail-safe mechanism to ensure the cotranscriptional recruitment of TRAMP to nascent intron-containing transcripts before or during pre-mRNA splicing, such that the subsequently generated spliced-out introns, abnormal splicing intermediates, or splicing-incompetent pre-mRNAs can be recognized immediately by TRAMP, and then targeted to the nuclear exosome for prompt degradation before their potentially harmful accumulation. Since most TRAMP and nuclear exosome components found in budding yeast also contain functional human homologs, this work provides important insights into how splicing side-products are rapidly degraded by the nuclear RNA quality control system in human cells, which have a much higher frequency of introns within their genome, and certainly require a much more efficient pathway for the removal of an increased amount of splicing side-products due to the greater number of splicing events. / published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
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Analysis of the molecular mechanisms underlying oskar mRNA localisation and axis formation during Drosophila oogenesisZimyanin, Vitaly Leonidovich January 2005 (has links)
No description available.
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Recognition of mRNA localization signals in Drosophila developmentDienstbier, Martin January 2010 (has links)
No description available.
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Protein-DNA recognition : in vitro evolution and characterization of DNA-binding proteins /Nilsson, Mikael, January 2004 (has links)
Diss. (sammanfattning) Uppsala : Univ., 2004. / Härtill 3 uppsatser.
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Chicken histone H5 mRNA and its genes /Scott, Andrew Charles. January 1975 (has links) (PDF)
Thesis (Ph.D.) -- University of Adelaide, Dept. of Biochemistry, 1976? / Typescript (photocopy).
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Chicken globin mRNA and its precursor /Crawford, Robert John. January 1977 (has links) (PDF)
Thesis (Ph.D.) -- University of Adelaide, Dept. of Biochemistry, 1977. / Typescript (photocopy).
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The use of [beta]-actin mRNA degradation to estimate bloodstain ageNestor, Kristin Nicole. January 2006 (has links)
Thesis (M.S.)--West Virginia University, 2006. / Title from document title page. Document formatted into pages; contains vi, 56 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 55-56).
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