Yes / Background: This study investigated predictors of response to topical diphenylyclopropenone (DPCP) immunotherapy in patients with alopecia areata (AA). Objective: To identify predictors of response, or resistance, to treatment for AA through clinical observations and serum tests. Methods: Eighty four AA patients were treated with DPCP. Serum cytokine levels were measured in 33 AA patients pre- and post-treatment, and in 18 healthy controls, using ELISA assays. Results: Of patients, 56.1% responded to DPCP with satisfactory hair regrowth; the response rate was negatively correlated with hair loss extent. Before DPCP treatment, higher serum IFN-γ and IL-12 cytokine levels were observed in AA patients compared to healthy controls. Non-responders to DPCP had significantly elevated serum IL-4 pre-treatment (3.07 fold higher) and lower IL-12 levels compared with responders. After DPCP treatment, non-responders had persistently high IL-4, increased IL-12, negligible decrease in IFN-γ and decreased IL-10. Post-treatment DPCP responders exhibited significantly decreased IFN-γ and IL-12, and increased IL-4 and IL-10. Development of adverse side-effects was significantly associated with higher pre-treatment serum IgE levels. Limitations: A small number of subjects were evaluated. Conclusions: Potentially, elevated pre-treatment serum levels of IL-4 and IL-12 can be used as unfavorable and favorable predictors of DPCP therapeutic effect, respectively. In addition, pre-treatment elevated serum total IgE may predict increased risk for severe adverse side-effects to DPCP application. Whether serum cytokine expression levels can be used as predictors of response to other forms of treatment is unknown, but it may warrant investigation in the development of personalized treatments for AA. / This work is supported by the National Natural Science Foundation of China (81573066) and Natural Science Foundation of Guangdong Province (2014A030313098) to Xingqi Zhang.

Cancer is one of the leading cause of mortality worldwide. Current anti-cancer therapeutics are approved for specific targets (e.g., ER, RAS, HER2). Despite initial favorable responses, cancer cells still acquire drug resistance and cross-tolerance to other treatments overtime. To address this urgent unmet need, new targets must be identified. Our research focuses on Chaperonin-containing TCP-1 (CCT), a double-ring, cylindrical protein folding complex consisting of eight subunits. Cancer patient databases revealed that higher gene expression of the second subunit, CCT2, correlates with tumor progression and poorer survival probability across various cancers. We hypothesize that cancer cells upregulate CCT2 to meet the demand for increased protein folding activity to produce essential products resulting from genomic alterations. Functionally, the depletion of CCT2 decreased cell viability and proliferation in T47D (luminal A breast cancer) cells and reduced tumor growth, nodule amounts, and CCT2 RNA in S-K-NAS (non-MYCN amplified neuroblastoma) tumors subcutaneously injected in nude mice. Conversely, the exogenous expression of CCT2 in T47D cells caused changes in cytoskeletal morphology (e.g. increased F-actin staining), greater migration, and larger sized spheroids compared to control cells. Through transcriptomic analysis, we found that depletion of CCT2 caused different patterns of transcriptomic changes in T47D compared to S-K-NAS cells, but showed similarities in decreased mRNA expression of other CCT subunits, molecular chaperones (e.g. HSP) and key oncogenic clients in major cancer signaling pathways. Gene ontology (GO) enrichment analysis showed that DNA processing and transcription could be implicated in breast cancer and cytoplasmic processes (e.g. protein localization, clathrin-coat assembly) are affected in neuroblastoma with CCT2 depletion. These results support that therapeutically targeting CCT for inhibition could target multiple oncogenic drivers, such as transcription factors and signaling kinases. As such, CCT inhibition could have a global impact on cancer cells without the need to pre-identify organ-specific genomic changes.

Photocopy of typescript. / Digitized by Kansas Correctional Industries

In this study the role played by metaphor in psychotherapy is investigated. Issues discussed, included the formulation of a definition of metaphor as well as an adequate theory of metaphor. The place metaphor holds in thought and learning; as well as how it has found its expression within some psychotherapeutic traditions are also discussed. The primary aim of the dissertation is to illustrate the versatility of metaphor, enabling it to be a significant and powerful tool in the hand of the psychotherapist. The application of various forms of metaphor is illustrated through case studies which offer a discussion of how the specific type of metaphor had been applied in psychotherapy. In conclusion, some recommendations are made with regard to further research within the fields of psychotherapy and neuropsychology. / Psychology / M.A. (Clinical Psychology)

Thesis (M.Tech.: Homoeopathy)--Durban University of Technology, 2008 / Introduction This dissertation entails a homoeopathic proving of ivory from the male African elephant (Loxodonta africana) 30CH with a subsequent comparison to Lac Loxodonta africana. Objectives The primary objective of this proving was to determine the effects of homoeopathically prepared ivory from the male African elephant (Loxodonta africana) in a 30CH dilution and was achieved by administering the remedy to a group of healthy individuals (provers) who will document all symptoms that arise as a result of taking the remedy. These symptoms will be used to identify the therapeutic indications of homoeopathic ivory. With these specific indications being documented the remedy can then be utilized in the sick individual, that present with similar symptoms, to induce a cure. A further objective of this proving is to report any variation that may exist in the comparison of two remedies, namely Lac Loxodonta africana (milk derived from the African elephant) and the remedy used in this proving, ivory from the male African elephant (Loxodonta africana). Methodology The substance was triturated up until the 3CH and subsequently converted into a liquid potency to be potentised up until the 30CH. Granules were then impregnated with the 30CH liquid potency. Ten impregnated granules were then placed in each individual ii lactose powder sachets. A total of six powders were dispensed to the proving participants. The proving was conducted as a double blind placebo controlled study with a total of twenty-six (26) provers that met the inclusion criteria (Appendix A). The group was made up of both homoeopathic students as well as the general public of varying ages, race and gender. The total group was randomly divided into two groups, twenty (20) of which received the homoeopathic remedy, the remainder (6) received placebo. A full case history of each prover was taken before commencing the proving as well as on completion of the study. Each individual prover kept a journal, starting a week before the proving, which was continued while taking the remedy and ceased when all symptoms had abated. Once all provers had completed the proving, the information received from the provers through the journals from both groups was collated, assessed and analyzed. A comparison was then made between this proving and Lac Loxodonta africana to assess whether any similarities or differences were evident. The comparison was made on symptom similarities and rubric analysis. Results The proving of ivory from the African elephant (Loxodonta africana) revealed a variety of symptoms. A total of 32 systems were affected in the twenty provers who received the remedy. 716 symptoms were recorded, 83 of which were new symptoms. The systems that were predominately affected were the mind, head and extremities. Many symptoms were confirmed to be similar to those identified in the proving of Lac Loxodonta africana, though differences were also acknowledged.

No description available.

Thesis (MSc)--Stellenbosch University, 2015. / ENGLISH ABSTRACT: Chronic inflammation is suggested to contribute to cancer development and therefore a potential target for chemoprevention. In the skin, keratinocytes and macrophages play an integral part in acute and chronic inflammation, with interleukin 1-α (IL-1α) and tumor necrosis factor α (TNF-α) as key cytokines governing this process. Green tea (Camellia sinensis) and the South African herbal teas, rooibos (Aspalathus linearis) and honeybush (Cyclopia spp.) displayed antiinflammatory effects in mouse and human skin. To further investigate the antiinflammatory properties of green tea and the herbal teas, rooibos and honeybush (C. subternata and C. maculata) herbal teas, suitable cell culture models were developed and validated utilising human keratinocytes (HaCaT) and monocyte (THP- 1) derived macrophages. Aqueous extracts of the green tea and unfermented herbal teas were prepared and their chemical composition determined by high performance liquid chromatography (HPLC) and the antioxidant activity characterised utilising different antioxidant assays. Green tea and rooibos exhibited similar antioxidant activities while C. maculata displayed the lowest overall antioxidant activity of all the extracts, despite possessing the highest mangiferin level, the major polyphenol in honeybush. The modulation of cytokine release was studied in (i) an UVB-induced pre-exposure HaCaT model monitoring the accumulation of IL-1α and (ii) a LPS stimulated THP-1 macrophage model monitoring the TNF-α release, utilising both a pre-exposure and co-exposure extract regimens. In the pre-exposure HaCaT inflammatory model the UVB-induced IL-1α was decreased by the green tea extract while a far weaker response was obtained with the rooibos extract. Both the honeybush extracts displayed a significant effect in the reduction of IL-1α with C. subternata exhibiting a slight increased protection at a lower extract concentration. In the pre-exposure THP-1 derived macrophage model, green tea and the herbal tea extracts inhibited TNF-α release in a dose dependent manner in the absence of an overt loss in cell viability and apoptosis at lower extract concentrations, suggesting a typical anti-inflammatory effect. In the co-exposure model, the different extracts also exhibited an anti-inflammatory effect at the lowest concentrations in the absence of apoptosis while at higher extract concentrations the effect was masked by a decrease in cell viability and increased apoptosis. C. maculata exhibit differential effects when considering the inhibition of cytokine production and, depending on the cell model, either exhibited a weaker or stronger effect when compared to C. subternata and rooibos. Phenolic diversity of the different teas is likely to explain the differential effects in the antioxidant assays and cell culture models with respect to the regulation of the production of the inflammatory markers. Proposed mechanism for the anti-inflammatory effects include the modulation of oxidative stress via various pathways and the subsequent down regulation of nuclear factor kappa β (NFκB) and activated protein-1 (AP-1) which are key regulators of cytokine production governing the inflammatory response. / AFRIKAANSE OPSOMMING: Kroniese inflammasie van die vel kan bydra tot die ontwikkeling van kanker en is dus ’n potensiële area om te teiken in die voorkoming van velkanker. Keratinosiete en makrofage speel ’n integrale rol in akute en chroniese inflammasie van die vel en TNF-α en IL-1α is die belangrikste sitokiene wat hierdie proses inisieer. Dit is bekend dat ekstrakte van groen tee (Camellia sinensis) en die Suid-Afrikaanse kruietees, rooibos (Aspalathus linearis) en heuningbos (Cyclopia spp.) ‘n anti-inflammatoriese effek op die vel van muise en mense het. Om die anti-inflammatoriese aktiwitieit van groen tee, rooibos en 2 heuningbos kruietees (C. subternata en C. maculata) verder te ondersoek en te definieer is geskikte selkultuurmodelle ontwikkel en gevalideer deur gebruik te maak van menslike keratinosiete (HaCaT) en monosiet (THP-1) afgeleide makrofage. Water ekstrakte van groen tee en ongefermenteerde kruietees is voorberei en die chemiese samestelling deur hoë druk vloeistof chromatografie (HDLC) bepaal. ‘n Verskeidenheid van antioksidant bepalingstoetse is gebruik om die antioksidant aktiwiteit van die ekstrakte te meet. Groen tee en rooibos het soortgelyke antioksidant aktiwiteite getoon, terwyl C. maculata die swakste algehele aktiwiteit getooon het, ten spyte van die teenwoordigheid van hoёr vlakke van mangiferin, die belangrikste polifenoliese verbinding in heuningbos. Modulasie van sitokiene is verder bestudeer in (i) ’n UVB-geïnduseerde vooraf-blootstelling HaCaT model, waartydens akkumulering van IL-1α gemonitor is en (ii) ‘n lipopolisakkaried (LPS)-gestimuleerde THP-1 makrofaag model, waar die vrystelling van TNF-α gemonitor is. Vir die THP-1 model is beide die voor en gelyktydige blootstelling benaderings vir die ekstrakte met LPS gebruik. In die keratinosiet model, waar die selle aan ekstrakte blootgestel is voor UVB bestraing, is IL-1α beduidend verlaag deur die groen tee ekstrak, terwyl ’n swakker reaksie gesien is met rooibos. Beide heuningbos ekstrakte het ’n beduidende invloed in die vermindering van IL-1α getoon, waar C. subternata ’n effense verhoogde beskerming teen selsterfte by ‘n laer ekstrakkonsentrasie toon. Blootstelling van die makrofage aan al vier ekstrakte voor LPS stimulasie (vooraf-blootstelling), het gelei tot inhibisie van TNF-α vrystelling op ’n dosis afhanklike wyse en die afwesigheid van apoptose en selsterftes by lae ekstrak konsentrasievlakke. Hierdie waarnemings dui op ’n tipiese antiinflammatoriese effek. In die gelyktydige-blootstelling model verlaag al die ekstrakte TNF-α vrystelling teen die laagste ekstrak konsentrasievlakke, in die afwesigheid van apoptose en met geen effek op seldood nie. Hoёr ekstrak konsentrasievlakke het sitotoksisiteit en verhoogde apoptose getoon, dus was die anti-inflammatoriese effek gemaskeer. C. maculata toon ‘n variërende effek met betrekking tot antioksidant aktiwiteit en die bekamping van sitokien produksie, afhangend van die model wat bestudeer is. Die verskeidenheid fenoliese verbindings teenwoordig in die verskillende tee ekstrakte is waarskynlik die rede vir die effekte wat waargeneem is tydens antioksidant toetsing en selkultuurmodelle. Die anti-inflammatoriese meganismes wat deur hierdie studie voorgestel word sluit die modulasie van oksidatiewe stres via verskeie metaboliese paaie in. Modulasie van oksidatiewe stres lei tot af-regulering van kernfaktor-kappaB (NF-κB) en aktiveerderproteïen- 1(AP-1), wat sleutel reguleerders van sitokien produksie tydens inflammatoriese respons is.

Thesis (MA)--University of Stellenbosch, 2002. / ENGLISH ABSTRACT: This study focuses on existing research into the field of animal-assisted therapy (AAT) and attempts to provide a clear answer as to what animal-assisted therapy (AAT) is. In addition, the limitations of current research, as well as future opportunities for research in this field and some practical considerations for applying animal-assisted therapy are explored. The origin of animal-assisted therapy is examined. How the present terminology has developed in that it defines the use of animals in therapy as an adjunct to other therapeutic techniques is discussed in contrast to previous terminology, which created the impression that there was some form of managed process on the part of the animal. The terminology has developed from terms such as pet therapy and pet-facilitated therapy to animal-assisted therapy (AAT) and animal-assisted activities (AAA). The history of animal-assisted therapy is examined in relation to the three therapy categories of milieu therapy, physical rehabilitation and animal-assisted psychotherapy. The most common theoretical frameworks for AAT are also discussed. In general, systems theory tends to be the most favoured theoretical foundation for AAT. The typical target populations of animal-assisted therapy are examined in the light of target relationships. The six target relationships that a practitioner of animal-assisted therapy would need to manage are identified and their merits discussed: therapist-and-patient relationship; therapist-and-animal relationship; the staff-and-patient and staff-and-animal relationship; the staff-and-animal therapist relationship; the animal-and-patient relationship; and the application environment wherein these relationships are lived. The typical research designs for AAT are also discussed within the history of AATand successful research tends toward longitudinal studies wherein patients with similar diagnostic profiles are all exposed to a common form of treatment. The experimental group has some form of AAT in addition to the standard treatment whilst the control group continues with only the standard treatment. Comparisons are made against specific measurements such as degree of sociability and other indices. In general, the current research indicates a need for research characterised by better controls and the application of general research principles to supplement the abundance of anecdotal and case study reports on AAT. In addition, the practical application of AAT is also examined in relation to training and liability, office management and décor, animal well-being, and the necessary precautions to safeguard patients from possible harm. A critique of AAT is provided as well as the difficulties encountered in the practical implementation of animal-assisted therapy. The literature reviewed for this study confirms that animal-assisted therapy shows excellent promise, which increases when complimented by experimental endeavour in terms of properly evaluated AAT programmes. In terms of the future potential of AAT, the possible advantages of the implementation of AAT programmes into schools, prisons and working environments is raised. Related therapeutic adjuncts such as horticultural and natural therapy are also discussed. Fine (2000) was the most up to date and encompassing source for AAT and may be a good tool to guide future practitioners and researchers in the field of AAT. / AFRIKAANSE OPSOMMING: DIERE-ONDERSTEUNDE TERAPIE (DOT) -WAT IS DIT? Hierdie studie fokus op huidige navorsing op die gebied van diere-ondersteunde terapie (DOT) en strewe om lig te gooi op wat presies diere-ondersteunde terapie is. Daarbenewens, word die beperkinge van huidige navorsing sowel as toekomstige geleenthede vir navorsing op hierdie gebied. Praktiese doelwitte vir die toepassing van diere-ondersteunde terapie is ook geidentifiseer. Die oorsprong van diere-ondersteunde terapie word ondersoek. Hoe die huidige terminologie ontwikkel het in sover dit die gebruik van diere aangaan in terapie as adjunk tot ander terapeutiese tegnieke word bespreek, in vergelyking met vorige terminologie wat die indruk geskep het dat daar een of ander bestuurde proses is wat deur die dier uitgevoer word. Die terminologie het ontwikkel van terme soos troeteldierterapie en troeteldier-gefasiliteerde terapie tot diere-ondersteunde terapie (DOT) en diereondersteunde aktiwiteite (DOA). Die geskiedenis van diere-ondersteunde terapie word ondersoek volgens die drie terapiekategoriee van milieuterapie, fisiese rehabilitasie en diere-ondersteunde psigoterapie. Die mees algemene teoretiese raamwerke vir DOT word ook bespreek. Oor die algemeen, is sisteemteorie die sigbaarste teoretiese grondslag vir DOT. Die tipiese teikengroepe vir diere-ondersteunde terapie word ondersoek in die lig van teiken verhoudings. Die ses teikenverhoudings wat 'n praktisyn van diere-ondersteunde terapie sou bestuur word onderskei en hul relatiewe meriete bespreek: die terapeut/pasient-verhouding; terapeut/dier-verhouding; personeel/pasient-verhouding; personeel/diereterapeut- verhouding; dier/pasient- verhouding ; sowel as die toepassings omgewing waarin die verhoudings uitgeleef word. Die tipiese navorsingsontwerpe vir DOT word ook binne die geskiedenis van DOT bespreek. Die mees geloofwaardige navorsing neig tot longitudinale studies waarin pasiente met soorgelyke diagnostiese profiele almal aan 'n gemene vorm van behandeling blootgestel is. Die eksperimentele groep kry dan een of ander vorm van DOT sowel as die standaard behandeling terwyl die kontrole groep slegs die standaard behandeling ontvang. Vergelykings word dan gemaak volgens spesifieke metings soos mate van sosialiteit en ander persoonlike effektiwiteit maatstawwe. Oor die algemeen dui huidige navorsing op 'n behoefte vir navorsing wat deur beter beheer gekenmerk word, en die toepassing van algemene navorsingsbegrippe om as aanvulling te dien tot die oorvloed anekdotiese en gevallestudies wat die DOT literatuur betref. Daarbenewens word die die praktiese toepassing van DOT ondersoek met betrekking tot opleiding en verantwoording, kantoorbeheer en dekor, dierewelsyn sowel as die nodige teenmaatreëls om pasiente teen enige negatiewe gevolge te beskerm. 'n Kritiese ontleding van DOT word ook voorsien en die moontlike struikelblokke wat in die praktiese implementasie van diere-ondersteunde terapie ondervind kan word. Die literatuur wat vir hierdie studie nagegaan is, bevestig dat diere-ondersteunde terapie uitstekende vooruitsigte toon. Sover dit die toekomstige potensiaal van DOT aangaan, word die moontlike voordele van die implementasie van DOT-programme in skole, tronke en werksomgewings genoem. Verwante terapeutiese byvoegings soos tuin- en natuur-terapie word ook bespreek. Fine (2000) blyk om die mees resente en omvattende bron van DOT te wees en mag 'n goeie hulpmiddel wees om toekomstige praktisyns en navorsers op die gebied van DOT van 'n riglyn te voorsien.

published_or_final_version / abstract / Chemistry / Doctoral / Doctor of Philosophy

Therapeutic Assessment (TA) is a semi-structured hybrid of assessment and intervention methods that aims to promote positive change in clients through collaboration. Studies have shown it to be an effective therapeutic intervention, but few studies have focused on adolescents. This comparative study examines the effects of TA, compared with assessment as usual, with an adolescent population. Dependent measures include symptom reduction and components of the assessment experience, specifically self-knowledge, feeling understood by the assessor, positive relationship with the assessor, and negative feelings about the assessment. A repeated measure ANOVA will examine the group effects on symptom reduction, while a MANOVA will be used to determine the effects of TA on the variables of assessment experience. / text